Detection of Inositol Polyphosphates by Polyacrylamide Gel Electrophoresis (PAGE) under Apoptotic Conditions in Cultured SW480 Cells

Inositol phosphates are naturally occurring compounds that regulate diverse cellular processes including apoptosis. Apoptosis is a mechanism by which cells undergo natural death to maintain cellular homeostasis. It causes cell death in areas during a state that is harmful to the body. It also regulates cellular development. Previous work has shown that exogenously administered, as well as endogenously manipulated inositol phosphates bring about apoptotic changes. It has been demonstrated that cellular levels of inositol phosphates, particularly higher inositol phosphates such as inositol hexakis-phosphate (IP6) and diphosphoinositol pentakis-phosphate (IP7) levels increase during apoptotic conditions. In this study, we have attempted to separate and identify higher inositol phosphates such as IP6 by polyacrylamide gel electrophoresis (PAGE) and shown that changes in inositol phosphate levels can be detected by this method. Cells were treated with etoposide to induce apoptosis, and apoptotic cells were observed under UV light following ethidium bromide/acridine orange staining. This staining showed that IP3 - IP6 induced apoptosis in SW480 cells with IP6 being the most effective inducing agent. The extracts from apoptotic and control cells were then loaded onto the polyacrylamide gel and run along with standard IP6. Results showed that IP6 could be detected using the PAGE method and that cellular levels of IP6 were increased in SW480 cells, in which apoptosis had been induced by etoposide. Our results demonstrated that this technique could be utilized instead of the laborious radioactive labeling and HPLC separation method to study the changes in cellular levels of inositol phosphates particularly IP6

The Achievable Rate of Interweave Cognitive Radio in the Face of Sensing Errors

Cognitive radio (CR) systems are potentially capable of mitigating the spectrum shortage of contemporary wireless systems. In this paper, we provide a brief overview of CR systems and the important research milestones of their evolution, along with their standardization activities, as a result of their research. This is followed by the detailed analysis of the interweave policy-based CR network (CRN) and by a detailed comparison with the family of underlay-based CRNs. In the interweave-based CRN, sensing of the primary user's (PU) spectrum by the secondary user's (SU) has remained a challenge, because the sensing errors prevent us from fulfilling the significant throughput gains that the concept of CR promises. Since missed detection and false alarm errors in real-time spectrum sensing cannot be avoided, based on a new approach, we quantify the achievable rates of the interweave CR by explicitly incorporating the effect of sensing errors. The link between the PU transmitter and the SU transmitter is assumed to be fast fading. Explicitly, the achievable rate degradation imposed by the sensing errors is analyzed for two spectrum sensing techniques, namely, for energy detection and for magnitude squared coherence-based detection. It is demonstrated that when the channel is sparsely occupied by the PU, the reusing techniques that are capable of simultaneously providing low missed detection and false alarm probabilities cause only a minor degradation to the achievable rates. Furthermore, based on the achievable rates derived for underlay CRNs, we compare the interweave CR and the underlay CR paradigms from the perspective of their resilience against spectrum sensing errors. Interestingly, in many practical regimes, the interweave CR paradigm outperforms the underlay CR paradigm in the presence of sensing errors, especially when the SNR at the SU is below 10 dB and when the SNR at the PU is in the range of 10-40 dB. Furthermore, we also provide rules of thumb that identify regimes, where the interweave CR outperforms the underlay CR

The SAMI Galaxy Survey: the intrinsic shape of kinematically selected galaxies

Using the stellar kinematic maps and ancillary imaging data from the Sydney AAO Multi Integral field (SAMI) Galaxy Survey, the intrinsic shape of kinematically-selected samples of galaxies is inferred. We implement an efficient and optimised algorithm to fit the intrinsic shape of galaxies using an established method to simultaneously invert the distributions of apparent ellipticities and kinematic misalignments. The algorithm output compares favourably with previous studies of the intrinsic shape of galaxies based on imaging alone and our re-analysis of the ATLAS3D data. Our results indicate that most galaxies are oblate axisymmetric. We show empirically that the intrinsic shape of galaxies varies as a function of their rotational support as measured by the "spin" parameter proxy Lambda_Re. In particular, low spin systems have a higher occurrence of triaxiality, while high spin systems are more intrinsically flattened and axisymmetric. The intrinsic shape of galaxies is linked to their formation and merger histories. Galaxies with high spin values have intrinsic shapes consistent with dissipational minor mergers, while the intrinsic shape of low-spin systems is consistent with dissipationless multi-merger assembly histories. This range in assembly histories inferred from intrinsic shapes is broadly consistent with expectations from cosmological simulations.Comment: 15 pages, 11 figures, MNRAS in prin

Distribution of finfish resources along southeast coast of India in relation to certain environmental parameters

This paper embodies the distribution pattern of major finfish resources along the southeast coast of India as observed during the cruises operated by FORV Sagar Sampada. A total of 16 cruises (1985-90) operated along latitude 7° 15'- 15°00' N and longitude 75°50'- 82°3r E hauled a total catch 37.5 tonnes with a catch rate of 537 kg/hr. The catch was constituted mainly by threadfin breams (43%), perches (14%), barracudas (9.72%), carangids (8.56%) and elasmobranchs (4.81%). Seasonally higher catch rates were obtained during July- September period. Depth range of 60 - 80 m had denser population of finfish resources. Water temperature and salinity appeared to influence the distribution of major finfishes more than dissolved oxygen. Groups such as threadfin breams were found preferring cooler waters of Wadge Bank area, while barracudas appear to occupy warmer waters of Gulf of Mannar

Occurrence of ribbonfish in the Indian EEZ

The survey conducted by FORV Sagar Sampada during 1985-91 (90 cruises) revealed the occurrence of ribbonfish in 56 stations out of 904 stations where fishing was conducted. It was reported from 41 stations along the west coast and 14 stations along the east coast. About 14 tonne of ribbonfish was caught in these operations which formed 4.8% of the total fish catch. West coast was more productive and contributed 93.6% of the catch, while the east coast only 6.4%. It abundantly occurred in the southwest, centralwest, northwest and northeast coasts. Depth-wise study revealed that major portion of the catch was obtained from within the 100 m depth zone. The dominant species reported was Trichiurus leptur

The mysterious optical afterglow spectrum of GRB140506A at z=0.889

Context. Gamma-ray burst (GRBs) afterglows probe sightlines to star-forming regions in distant star-forming galaxies. Here we present a study of the peculiar afterglow spectrum of the z = 0.889 Swift GRB 140506A. Aims. Our aim is to understand the origin of the very unusual properties of the absorption along the line-of-sight. Methods. We analyse spectroscopic observations obtained with the X-shooter spectrograph mounted on the ESO/VLT at two epochs 8.8 h and 33 h after the burst as well as imaging from the GROND instrument. We also present imaging and spectroscopy of the host galaxy obtained with the Magellan telescope. Results. The underlying afterglow appears to be a typical afterglow of a long-duration GRB. However, the material along the line-of- sight has imprinted very unusual features on the spectrum. Firstly, there is a very broad and strong flux drop below 8000 AA (4000 AA in the rest frame), which seems to be variable between the two spectroscopic epochs. We can reproduce the flux-drops both as a giant 2175 AA extinction bump and as an effect of multiple scattering on dust grains in a dense environment. Secondly, we detect absorption lines from excited H i and He i. We also detect molecular absorption from CH+ . Conclusions. We interpret the unusual properties of these spectra as reflecting the presence of three distinct regions along the line-of-sight: the excited He i absorption originates from an H ii-region, whereas the Balmer absorption must originate from an associated photodissociation region. The strong metal line and molecular absorption and the dust extinction must originate from a third, cooler region along the line-of-sight. The presence of (at least) three separate regions is reflected in the fact that the different absorption components have different velocities relative to the systemic redshift of the host galaxy.Comment: 8 pages, 4 figures. Accepted for publications in A&

Association Between Residential Greenness and Cardiovascular Disease Risk

Background Exposure to green vegetation has been linked to positive health, but the pathophysiological processes affected by exposure to vegetation remain unclear. To study the relationship between greenness and cardiovascular disease, we examined the association between residential greenness and biomarkers of cardiovascular injury and disease risk in susceptible individuals. Methods and Results In this cross-sectional study of 408 individuals recruited from a preventive cardiology clinic, we measured biomarkers of cardiovascular injury and risk in participant blood and urine. We estimated greenness from satellite-derived normalized difference vegetation index ( NDVI ) in zones with radii of 250 m and 1 km surrounding the participants' residences. We used generalized estimating equations to examine associations between greenness and cardiovascular disease biomarkers. We adjusted for residential clustering, demographic, clinical, and environmental variables. In fully adjusted models, contemporaneous NDVI within 250 m of participant residence was inversely associated with urinary levels of epinephrine (-6.9%; 95% confidence interval, -11.5, -2.0/0.1 NDVI ) and F2-isoprostane (-9.0%; 95% confidence interval, -15.1, -2.5/0.1 NDVI ). We found stronger associations between NDVI and urinary epinephrine in women, those not on β-blockers, and those who had not previously experienced a myocardial infarction. Of the 15 subtypes of circulating angiogenic cells examined, 11 were inversely associated (8.0-15.6% decrease/0.1 NDVI ), whereas 2 were positively associated (37.6-45.8% increase/0.1 NDVI ) with contemporaneous NDVI . Conclusions Independent of age, sex, race, smoking status, neighborhood deprivation, statin use, and roadway exposure, residential greenness is associated with lower levels of sympathetic activation, reduced oxidative stress, and higher angiogenic capacity

Fairness in Algorithmic Decision Making: An Excursion Through the Lens of Causality

As virtually all aspects of our lives are increasingly impacted by algorithmic decision making systems, it is incumbent upon us as a society to ensure such systems do not become instruments of unfair discrimination on the basis of gender, race, ethnicity, religion, etc. We consider the problem of determining whether the decisions made by such systems are discriminatory, through the lens of causal models. We introduce two definitions of group fairness grounded in causality: fair on average causal effect (FACE), and fair on average causal effect on the treated (FACT). We use the Rubin-Neyman potential outcomes framework for the analysis of cause-effect relationships to robustly estimate FACE and FACT. We demonstrate the effectiveness of our proposed approach on synthetic data. Our analyses of two real-world data sets, the Adult income data set from the UCI repository (with gender as the protected attribute), and the NYC Stop and Frisk data set (with race as the protected attribute), show that the evidence of discrimination obtained by FACE and FACT, or lack thereof, is often in agreement with the findings from other studies. We further show that FACT, being somewhat more nuanced compared to FACE, can yield findings of discrimination that differ from those obtained using FACE.Comment: 7 pages, 2 figures, 2 tables.To appear in Proceedings of the International Conference on World Wide Web (WWW), 201

An Introduction to Gas Accretion onto Galaxies

Evidence for gas accretion onto galaxies can be found throughout the universe. In this chapter, I summarize the direct and indirect signatures of this process and discuss the primary sources. The evidence for gas accretion includes the star formation rates and metallicities of galaxies, the evolution of the cold gas content of the universe with time, numerous indirect indicators for individual galaxies, and a few direct detections of inflow. The primary sources of gas accretion are the intergalactic medium, satellite gas and feedback material. There is support for each of these sources from observations and simulations, but the methods with which the fuel ultimately settles in to form stars remain murky.Comment: 14 pages, 5 figures, Invited review to appear in Gas Accretion onto Galaxies, Astrophysics and Space Science Library, eds. A. J. Fox & R. Dav\'e, to be published by Springe