Wavelet filtering of the P300 component in event-related potentials.

This paper presents an application of wavelet filtering to single-trial P300 component analysis. The objective of this study is to introduce a new method for analyzing the P300 component, when performing a given cognitive task, in this case, a two-choice reaction time task. The discrete wavelet transform with Daubechies wavelet is employed to detect the presence of P300 in individual trials. Wavelet filtering is applied to remove noise and unwanted frequency components from discrete wavelet transform (DWT) coefficients based on prior knowledge of event-related potentials (ERPs). The filtering mask is computed from the grand-average of wavelet coefficients over all participants. With this filtering, the P300 component is accurately localized in both time and scale. The findings suggest the procedure to have considerable potential for the analysis of time-series data in the behavioral neurosciences

Wavelet filtering of the P300 component in event-related potentials.

This paper presents an application of wavelet filtering to single-trial P300 component analysis. The objective of this study is to introduce a new method for analyzing the P300 component, when performing a given cognitive task, in this case, a two-choice reaction time task. The discrete wavelet transform with Daubechies wavelet is employed to detect the presence of P300 in individual trials. Wavelet filtering is applied to remove noise and unwanted frequency components from discrete wavelet transform (DWT) coefficients based on prior knowledge of event-related potentials (ERPs). The filtering mask is computed from the grand-average of wavelet coefficients over all participants. With this filtering, the P300 component is accurately localized in both time and scale. The findings suggest the procedure to have considerable potential for the analysis of time-series data in the behavioral neurosciences

The Role of Bacteria in KSHV Infection and KSHV-Induced Cancers

The objective of this article is to review the current status of the bacteria-virus interplay in Kaposi’s sarcoma-associated herpesvirus (KSHV) infection and KSHV-driven cancers. KSHV is the etiological agent of several cancers, including Kaposi’s sarcoma (KS) and primary effusion lymphoma. Due to immunosuppression, patients with KSHV are at an increased risk for bacterial infections. Moreover, among patients coinfected by HIV and KSHV, patients with KS have distinct oral microbiota compared to non-KS patients. Bacterial biomarkers associated with KSHV-driven cancers can provide insights in discerning the mechanisms of KSHV-induced oncogenesis. For example, pathogen-associated molecular patterns and bacterial products of certain bacterial species can regulate the expression of KSHV lytic and latent genes, thereby affecting viral replication and dissemination. In addition, infection with distinct opportunistic bacterial species have been associated with increased cell proliferation and tumorigenesis in KSHV-induced cancers through activation of pro-survival and -mitogenic cell signaling pathways. By elucidating the various mechanisms in which bacteria affect KSHV-associated pathogenesis, we will be able to pinpoint therapeutic targets for KSHV infection and KSHV-related cancers

The Role of Bacteria in KSHV Infection and KSHV-Induced Cancers.

The objective of this article is to review the current status of the bacteria-virus interplay in Kaposi's sarcoma-associated herpesvirus (KSHV) infection and KSHV-driven cancers. KSHV is the etiological agent of several cancers, including Kaposi's sarcoma (KS) and primary effusion lymphoma. Due to immunosuppression, patients with KSHV are at an increased risk for bacterial infections. Moreover, among patients coinfected by HIV and KSHV, patients with KS have distinct oral microbiota compared to non-KS patients. Bacterial biomarkers associated with KSHV-driven cancers can provide insights in discerning the mechanisms of KSHV-induced oncogenesis. For example, pathogen-associated molecular patterns and bacterial products of certain bacterial species can regulate the expression of KSHV lytic and latent genes, thereby affecting viral replication and dissemination. In addition, infection with distinct opportunistic bacterial species have been associated with increased cell proliferation and tumorigenesis in KSHV-induced cancers through activation of pro-survival and -mitogenic cell signaling pathways. By elucidating the various mechanisms in which bacteria affect KSHV-associated pathogenesis, we will be able to pinpoint therapeutic targets for KSHV infection and KSHV-related cancers

A knowledge map for hospital performance concept: Extraction and analysis: A narrative review article

Background: Performance is a multi-dimensional and dynamic concept. During the past 2 decades, considerable studies were performed in developing the hospital performance concept. To know literature key concepts on hospital performance, the knowledge visualization based on co-word analysis and social network analysis has been used. Methods: Documents were identified through �PubMed� searching from1945 to 2014 and 2350 papers entered the study after omitting unrelated articles, the duplicates, and articles without abstract. After pre-processing and preparing articles, the key words were extracted and terms were weighted by TF-IDF weighting schema. Support as an interestingness measure, which considers the co-occurrence of the extracted keywords and "hospital performance" phrase was calculated. Keywords having high support with "hospital performance" are selected. Term-term matrix of these selected keywords is calculated and the graph is extracted. Results: The most high frequency words after �Hospital Performance� were �mortality� and �efficiency�. The major knowledge structure of hospital performance literature during these years shows that the keyword �mortality� had the highest support with hospital performance followed by �quality of care�, �quality improvement�, �discharge�, �length of stay� and �clinical outcome�. The strongest relationship is seen between �electronic medical record� and �readmission rate�. Conclusion: Some dimensions of hospital performance are more important such as �efficiency�, �effectiveness�, �quality� and �safety� and some indicators are more highlighted such as �mortality�, �length of stay�, �readmission rate� and �patient satisfaction�. In the last decade, some concepts became more significant in hospital performance literature such as �mortality�, �quality of care� and �quality improvement�. © 2016, Iranian Journal of Public Health. All rights reserved

Surface engineered polyanhydride-based oral Salmonella subunit nanovaccine for poultry

Sankar Renu,1,2,* Ashley D Markazi,3,* Santosh Dhakal,1,2 Yashavanth S Lakshmanappa,1,2 Suren R Gourapura,1,2 Revathi Shanmugasundaram,3 Sujata Senapati,4 Balaji Narasimhan,4 Ramesh K Selvaraj,5 Gourapura J Renukaradhya1,2 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA; 2Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA; 3Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA; 4Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA; 5Department of Poultry Science, University of Georgia, Athens, GA 30602, USA *These authors contributed equally to this work Purpose: Salmonellosis is a severe economic threat in poultry and a public health concern. Currently available vaccines are ineffective, and thus, developing effective oral Salmonella vaccine is warranted. Especially, a potent oral vaccine such as the mucoadhesive polyanhydride nanoparticle (PNP) protects the vaccine cargo and delivers to intestinal immune sites to elicit robust mucosal immunity and mitigate Salmonella colonization and shedding.Materials and methods: We designed a Salmonella subunit vaccine using PNP containing immunogenic Salmonella outer membrane proteins (OMPs) and flagellar (F) protein-entrapped and surface F-protein-coated PNPs (OMPs-F-PNPs) using a solvent displacement method. Using high-throughput techniques, we characterized the OMPs-F-PNPs physicochemical properties and analyzed its efficacy in layer birds vaccinated orally.Results: The candidate vaccine was resistant in acidic microenvironment and had ideal physicochemical properties for oral delivery in terms of particle size, charge, morphology, biocompatibility, and pH stability. In vitro, in vivo, and ex vivo studies showed that F-protein surface-anchored nanoparticles were better targeted to chicken immune cells in peripheral blood and splenocytes and intestinal Peyer’s patch sites. In layer chickens inoculated orally with OMPs-F-PNPs, substantially higher OMPs-specific IgG response and secretion of Th1 cytokine IFN-γ in the serum, enhanced CD8+/CD4+ cell ratio in spleen, and increased OMPs-specific lymphocyte proliferation were observed. OMPs-F-PNPs vaccination also upregulated the expression of toll-like receptor (TLR)-2 and -4, TGF-β, and IL-4 cytokines’ genes in chicken cecal tonsils (lymphoid tissues). Importantly, OMPs-F-PNPs vaccine cleared Salmonella cecal colonization in 33% of vaccinated birds.Conclusion: This pilot in vivo study demonstrated the targeted delivery of OMPs-F-PNPs to ileum mucosal immune sites of chickens and induced specific immune response to mitigate Salmonella colonization in intestines. Keywords: Salmonella antigens, polyanhydride nanoparticles, oral delivery, ileum, chickens&nbsp