Lead-arsenic soil geochemical study as an exploration guide over the Killik volcanogenic massive sulfide deposit, Northeastern Turkey

WOS: 000230576800003A mountainous terrain, the eastern Pontide tectonic belt, located in northeastern Turkey, contains more than 60 known volcanogenic massive sulfide (VMS) deposits that differ in reserves (0.1-30 million tonnes) and grades. Soil geochemistry is conventionally used in exploration programs to discover concealed VMS deposits in the region. In the present study, Pb and As element pair were used as pathfinder elements to investigate the relationship of their anomalies to a completely delineated ore deposit (Killik VMS deposit) in an orientation survey that served as a natural physical model. Two hundred forty soil samples were analyzed in the present study. The two elements, which represent the opposite ends of the mobility range, revealed high contrast and overlapped each other at the location of the ore deposit due to enhancement of the anomalies by hydromorphic dispersion, which is an indication that soil samples would produce reliable results. The successful delineation of the deposit is remarkable considering the rough topography and the climatic limitations. Previously the extremely moist and temperate climate was thought to cause excessive leaching of the trace element pathfinders from the ore deposits to produce extensive anomalies usually extending away from the mineralization thus, leading to erroneous results and/or extensive anomalous areas. But the present research has shown that the method can be used effectively if the sampling and data evaluation is carefully conducted. (C)\ 2005 Elsevier B.V. All rights reserved

Chemical analysis and biological activities of essential oils from trunk-barks of eight trees

The present study was conducted to evaluate chemical compositions and antimicrobial and antioxidant activities of essential oils from trunk barks of Eucalyptus globulus, Juniperus oxycedrus L, Pinus nigra, Cedrus libani A. Rich, Abies equi-trojani, Cupressus sempervirens, Juglans regia and Alnus glutinosa Mill. The chemical composition of hydro-distilled essential oils of the 8 trunk-bark samples was analyzed by gas chromatography-mass spectrometry. E. globulus, J. oxycedrus, P. nigra, C. libani, A. equi-trojani, C. sempervirens, J. regia and A. glutinosa 56,52,34,56,57,46,13 and 14 components were identified in the essential oils of the plants mentioned above, respectively. Anti- oxidant activities were measured employing free radical, 2,2-diphenyl- 1-picrylhydrazyl (DPPH) scavenging ability of the samples. All essential oils tested showed moderate free radical scavenging activity. The essential oil of C. sempervirens showed the highest scavenging activity (SC5o: 70 ?g mL-1) while that of A. equi-trojani showed the lowest (SC 50: 5480 ?g mL'). The antimicrobial activity was studied by the agar diffu- sion method using 5 bacteria and a yeast-like fungus. The essential oils of the 8 species extended significant activity against C. tropicalis. The essential oil of C. libani was particularly active against the 5 bacteria studied with an minimal inhibitory concentration (MIC) at 250 ?g mL"1 inactive

Evaluation of phenolic compounds in Tilia rubra Subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS

Profile of phenolic compounds of Tilia rubra subsp. caucasica was measured by high performance liquid chromatography coupled with ultraviolet and tandem mass spectroscopy. Three different extraction methods (methonolic, selective extraction, and acidic hydrolysis) were used to evaluate phenolic composition and antioxidant capacity in three different parts of T. rubra. The antioxidant activities of the species were investigated in terms of total phenolics and flavonoids, and cupric reducing antioxidant capacity and 1,1-diphenyl-2- picrylhydrazyl scavenging assays. Different phenolic compounds related to antioxidant activities of three different parts and three different extraction ways of T. rubra were determined by high performance liquid chromatography- ultraviolet and high performance liquid chromatography-mass spectroscopy. Gallic and protocatechuic acid were the main phenolic compounds in the all extracts and parts of Tilia rubra subsp. caucasica by high performance liquid chromatography-ultraviolet ranging from 356.20 to 159.83 and 1873.90 to 720.80 ?g phenolic compound/g dry sample, respectively. Epicatechin, luteolin, and rhamnazin were detected by high performance liquid chromatography-mass spectroscopy. © 2014 Copyright Taylor and Francis Group, LLC.Huseyin S¸ahin would like to thank TUB?TAK B?DEB for the financial support given to him. The authors would also like to thank Professor Salih Terziog?lu for identifying the tilia genus and Faculty of Pharmacology, Karadeniz Technical University for helping with the HPLC device

KARAÇALI (Paliurus spina-christi Mill.) BALININ KARAKTERISTIK ÖZELLIKLERI

In this study characteristic properties of Jerusalem thorn, Christ's thorn or Garland thorn honey (Paliurus spina-christi Mill.) were investigated that obtained from different locations of Marmara and Trakya regions of Turkey. Eighteen honey samples were collected from Bursa, Edirne and Kirklareli regions by experienced beekeepers in 2018. Melissopalynological analysis, physicochemical parameters, pH, moisture, color, conductivity and optical rotation values, chemical parameters, proline, sugar components, total phenolic, flavonoid, tannin amounts and phenolic profile analyzes were analyzed of the honey samples Antioxidant and antimicrobial activities were tested as biological activities. According to the results of the study, it was found that the honey has a highly monofloral properties ranged from 69.5% and 96%. Antimicrobial activity values of the honey were compared with standard antibiotics and two different Manuka honeys. It was found that the honeys were showed higher antimicrobial and antifungal activities against 10 different pathogenic than Manuka honeys. As a result, it is thought that Jerusalem thorn honey have high biological active potential as monofloral honey for apitherapy applications. Copyright © 2019 Business Lawyer. All rights reserved.Scopu

Anti-inflammatory activities of some bee products by inhibition of bovine testes hyaluronidase

Background: Apitherapeutic products contain many valuable bioactive compounds which are responsible for biologically active features such as anti-oxidant, anti-viral, anti-microbial and anti-inflammatory properties. Objective: This study investigated in vitro anti-inflammatory potentials in terms of hyaluronidase inhibition of three different bee products, propolis, pollens and royal jellies. Methods: Water soluble extracts of the samples were prepared, and inhibitions of bovine serum hyaluronidase were used. Inhibitions were expressed as percentages of inhibition (IC50;?g/mL or mg/mL) causing 50% inhibition of the enzyme. Results: All the bee products exhibited anti-hyaluronidase activities in the order propolis, pollen, and finally royal jelly. Inhibition was also correlated with the samples’ total phenolic contents. Conclusion: The study results confirm that water-soluble propolis is the best inhibitory agent among the bee products, and that these may be used as anti-inflammatory agents. However, further in vivo studies are now needed to clarify their pharmaceutical potentials as medicines. © 2016 Bentham Science Publishers

Determination of chemical, physical and biological characteristics of some pekmez (Molasses) from Turkey

Antioxidant capacity, chemical and physical properties of 6 certificated and 1 uncertificated pekmez (molasses) samples of Turkish varieties were analyzed. All pekmez samples had similar chemical compositions. Total phenolic contents varied from 138 to 243 mg of gallic acid equivalents/100 g samples. The apricot pekmez had the highest phenolic content (243 ± 16), while the carob pekmez exhibited the highest DPPH (0.08 ± 0.01 mg g-1) radical scavenging activity. DPPH radical scavenging activity was also found to be related to concentrations of the samples. Especially, selenium and other minerals content were found slightly higher in grape pekmez than the others. Present results showed that all the certificated and uncertificated pekmez samples had no any risk in public health and besides their high content of sugar. Each pekmez had antioxidant and high amount of polyphenols and minerals

Characterization and inhibition studies of an α-carbonic anhydrase from the endangered sturgeon species Acipenser gueldenstaedti

An α-carbonic anhydrase (CA, EC 4.2.1.1) was purified and characterized kinetically from erythrocytes of the sturgeon Acipenser gueldenstaedti, an endangered species. The sturgeon enzyme (AgCA) showed kinetic parameters for the CO(2) hydration reaction comparable with those of the human erythrocytes enzyme hCA II, being a highly active enzyme, whereas its esterase activity with 4-nitrophenyl acetate as substrate was lower. Sulphonamide inhibitors (acetazolamide, sulphanilamide) strongly inhibited AgCA, whereas metal ions (Ag(+), Zn(2+), Cu(2+) and Co(2+)) were weak, millimolar inhibitors. Several widely used pesticides (2,4-dichlorophenol, dithiocarbamates, parathion and carbaryl) were also assayed as inhibitors of this enzyme. The dithiocarbamates were low micromolar AgCA inhibitors (IC(50) of 16-18 μM), whereas the other pesticides inhibited the enzyme with IC(50)s in the range of 102-398 μM. The wide use of dithiocarbamate pesticides may be one of the factors enhancing the vulnerability of this sturgeon species to pollutants

Investigation of some biological active properties of Anzer bee-pollen

41st FEBS Congress on Molecular and Systems Biology for a Better Life -- SEP 03-08, 2016 -- Kusadasi, TURKEYWOS: 000383616901875[No abstract available]FEB

Expression stability of six housekeeping genes: a proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR

Constantly expressed genes are used as internal controls in relative quantification studies. Suitable internal controls for such studies have not yet been defined for Pseudomonas aeruginosa. In this study, the genes ampC, fabD, proC, pbp-2, rpoD and rpoS of P. aeruginosa were compared in terms of expression stability by real-time quantitative RT-PCR. A total of 23 strains with diverse resistance phenotypes were studied. Stability of expression among the housekeeping genes was assessed on the basis of correlation coefficients, with the best-correlated pair accepted as being the most stable one. Eventually, proC and rpoD formed the most stable pair (r = 0.958; P < 0.001). Next, in four ciprofloxacin-selected nfxC-like mutants, levels of oprD, oprM and oprN mRNA were compared with those of their wild-type counterparts. The comparison was made after correcting the raw values by the geometric mean of the internal control genes proC and rpoD. The level of oprN mRNA was significantly up-regulated, while the oprD gene was down-regulated (although this difference was statistically insignificant), in the mutants. This expression pattern was consistent with that of the expected expression profile of nfxC-type mutants; this experiment therefore ends further support to the use of proC and rpoD genes simultaneously as internal controls for such studies