28 research outputs found
In vitro effects of 0 to 120 Grays of irradiation on bone viability and release of growth factors
Potential of radiosensitizing agents in cancer chemo-radiotherapy
Potential of herbs and other plant-based formulations have been
increasingly recognized in prevention and treatment of human diseases
including cancer. There exist enormous prospect for screening and
evaluation of herbal/plant products for developing effective
radiosensitization and radioprotection relevant to nuclear research
program. Investigations in our laboratory have focused on the mechanism
of activity of variety of anticancer and antioxidant agents, namely,
Eugenol, (EU), Ellagic acid (EA), Triphala (TPL), Tocopherol Succinate
(TOS) and Arachidonic acid on normal and cancer cells with view to
design effective protocols in practical radioprotection and cancer
radiotherapy. This paper is mainly focused on studies on cytotoxic
effects on cancer cell lines. Results have shown that these agents
produced radiosensitizing action involving oxidative damage, membrane
alteration and damage to nucleic acid in various human cell lines.
Studies were performed employing fluorescence probes and electron spin
resonance methods and gel electrophoresis protocols. It has been found
that cytotoxic effect was induced by initiating membrane oxidative
damage and by triggering intracellular generation of reactive oxygen
species (ROS) by gamma radiation in combination with phytochemicals
like TPL, EA and TOS in tumor cell line Ehrlich Ascites (EAC), Human
cervical (HeLa) and breast (MCF-7) cells. Membrane damage and ROS
generation was measured by DPH and DCF-FDA fluorescent probes
respectively after exposure to low to moderate doses of gamma
radiation. This talk will present the cytotoxic effects of
phytochemicals in combination with ionizing radiation. It is emphasized
that modulation of membrane peroxidative damage and intra cellular ROS
may help achieve efficient killing of cancer cells which may provide a
new approach to developing effective treatment of cancer
Potential of radiosensitizing agents in cancer chemo-radiotherapy
Potential of herbs and other plant-based formulations have been
increasingly recognized in prevention and treatment of human diseases
including cancer. There exist enormous prospect for screening and
evaluation of herbal/plant products for developing effective
radiosensitization and radioprotection relevant to nuclear research
program. Investigations in our laboratory have focused on the mechanism
of activity of variety of anticancer and antioxidant agents, namely,
Eugenol, (EU), Ellagic acid (EA), Triphala (TPL), Tocopherol Succinate
(TOS) and Arachidonic acid on normal and cancer cells with view to
design effective protocols in practical radioprotection and cancer
radiotherapy. This paper is mainly focused on studies on cytotoxic
effects on cancer cell lines. Results have shown that these agents
produced radiosensitizing action involving oxidative damage, membrane
alteration and damage to nucleic acid in various human cell lines.
Studies were performed employing fluorescence probes and electron spin
resonance methods and gel electrophoresis protocols. It has been found
that cytotoxic effect was induced by initiating membrane oxidative
damage and by triggering intracellular generation of reactive oxygen
species (ROS) by gamma radiation in combination with phytochemicals
like TPL, EA and TOS in tumor cell line Ehrlich Ascites (EAC), Human
cervical (HeLa) and breast (MCF-7) cells. Membrane damage and ROS
generation was measured by DPH and DCF-FDA fluorescent probes
respectively after exposure to low to moderate doses of gamma
radiation. This talk will present the cytotoxic effects of
phytochemicals in combination with ionizing radiation. It is emphasized
that modulation of membrane peroxidative damage and intra cellular ROS
may help achieve efficient killing of cancer cells which may provide a
new approach to developing effective treatment of cancer
The evaluation of radio-sensitivity of mung bean proteins aqueous extract on MCF-7, hela and fibroblast cell line
The Combination of Particle Irradiation With the Hedgehog Inhibitor GANT61 Differently Modulates the Radiosensitivity and Migration of Cancer Cells Compared to X-Ray Irradiation
Enhancement of IUdR Radiosensitization in Cancer Therapy by Low-Energy Transmission X Ray Irradiation
Dose-and time-dependent gene expression alterations in prostate and colon cancer cells after in vitro exposure to carbon ion and X-irradiation
Hadrontherapy is an advanced form of radiotherapy that uses beams of charged particles (such as protons and carbon ions). Compared with conventional radiotherapy, the main advantages of carbon ion therapy are the precise absorbed dose localization, along with an increased relative biological effectiveness (RBE). This high ballistic accuracy of particle beams deposits the maximal dose to the tumor, while damage to the surrounding healthy tissue is limited. Currently, hadrontherapy is being used for the treatment of specific types of cancer. Previous in vitro studies have shown that, under certain circumstances, exposure to charged particles may inhibit cell motility and migration. In the present study, we investigated the expression of four motility-related genes in prostate (PC3) and colon (Caco-2) cancer cell lines after exposure to different radiation types. Cells were irradiated with various absorbed doses (0, 0.5 and 2 Gy) of accelerated (13)C-ions at the GANIL facility (Caen, France) or with X-rays. Clonogenic assays were performed to determine the RBE. RT-qPCR analysis showed dose- and time-dependent changes in the expression of CCDC88A, FN1, MYH9 and ROCK1 in both cell lines. However, whereas in PC3 cells the response to carbon ion irradiation was enhanced compared with X-irradiation, the effect was the opposite in Caco-2 cells, indicating cell-type-specific responses to the different radiation types