Components required for in vitro cleavage and polyadenylation of eukaryotic mRNA

We have studied in vitro cleavage/polyadenylation of precursor RNA containing herpes simplex virus type 2 poly A site sequences and have analysed four RNA/protein complexes which form during in vitro reactions. Two complexes, A and B, form extremely rapidly and are then progressively replaced by a third complex, C which is produced following cleavage and polyadenylation of precursor RNA. Substitution of ATP with cordycepin triphosphate prevents polyadenylation and the formation of complex C however a fourth complex, D, results which contains cleaved RNA. A precursor RNA lacking GU-rich downstream sequences required for efficient cleavage/ polyadenylation fails to form complex B and produces a markedly reduced amount of complex A. As these GU-rich sequences are required for efficient cleavage, this establishes a relationship between complex B formation and cleavage/polyadenylation of precursor RNA in vitro. The components required for in vitro RNA processing have been separated by fractionation of the nuclear extract on Q-Sepharose and Biorex 70 columns. A Q-Sepharose fraction forms complex B but does not process RNA. Addition of a Biorex 70 fraction restores cleavage activity at the poly A site but this fraction does not appear to contribute to complex formation. Moreover, in the absence of polyethylene glycol, precursor RNA is not cleaved and polyadenylated, however, complexes A and B readily form. Thus, while complex B is necessary for in vitro cleavage and polyadenylation, it may not contain all the components required for this processing

S-1C Stage Instrumentation Systems Checkout at KSC

This paper will identify the test philosophies, describe the S-1C instrumentation systems and associated GSE, and define the test and checkout methods used at KSC, for S-1C test and checkout operations

Stable Kalman filters for processing clock measurement data

Kalman filters have been used for some time to process clock measurement data. Due to instabilities in the standard Kalman filter algorithms, the results have been unreliable and difficult to obtain. During the past several years, stable forms of the Kalman filter have been developed, implemented, and used in many diverse applications. These algorithms, while algebraically equivalent to the standard Kalman filter, exhibit excellent numerical properties. Two of these stable algorithms, the Upper triangular-Diagonal (UD) filter and the Square Root Information Filter (SRIF), have been implemented to replace the standard Kalman filter used to process data from the Deep Space Network (DSN) hydrogen maser clocks. The data are time offsets between the clocks in the DSN, the timescale at the National Institute of Standards and Technology (NIST), and two geographically intermediate clocks. The measurements are made by using the GPS navigation satellites in mutual view between clocks. The filter programs allow the user to easily modify the clock models, the GPS satellite dependent biases, and the random noise levels in order to compare different modeling assumptions. The results of this study show the usefulness of such software for processing clock data. The UD filter is indeed a stable, efficient, and flexible method for obtaining optimal estimates of clock offsets, offset rates, and drift rates. A brief overview of the UD filter is also given

A versatile wind-resistant insect cage

Ecological field studies often require cages that can withstand adverse weather conditions such as high winds, without greatly altering environmental conditions within them. A large field cage was designed, fabricated and tested for predator-prey studies on raspberry plantings. It consisted of a wood base and screening suspended with loops of canvas from a framework of PVC pipe. The cage withstood gusts above 70 km/h, did not appreciably alter temperature or RH, but did reduce light by 40% and rainfall by 25%. The cage design is simple and can be adapted to many experimental situations

High spatial resolution observations of CUDSS14A: a SCUBA-selected ultraluminous galaxy at high redshift

The definitive version is available at www.blackwell-synergy.com '. Copyright Blackwell Publishing DOI : 10.1046/j.1365-8711.2000.03822.xWe present a high-resolutionmillimetre interferometric image of the brightest SCUBA- selected galaxy from the Canada-UK deep SCUBA survey (CUDSS). We make a very clear detection at 1.3 mm, but fail to resolve any structure in the source.Peer reviewe

Souvenir Book of Bar Harbor, Me.

Colored, postcard-like images in a souvenir booklet showing scenes around Bar Harbor, Maine, published between 1915 and 1925. Images include the Village Green, Thunder Cave, Duck Brook, Frenchman\u27s Bay, Newport Mountain from Gorge Road, Bar Harbor High School, Balance Rock, Main Street, Shore Path, Bar Harbor from Rodick Island, the Post Office, Jesup Memorial Library and Y.W.C.A., Kebo Street, Profile Rock and a horse drawn buggy driving past Otter Cliff

Diffusion Monte Carlo study of two-dimensional liquid 4^4He

The ground-state properties of two-dimensional liquid $^4$He at zero temperature are studied by means of a quadratic diffusion Monte Carlo method. As interatomic potential we use a revised version of the HFDHE2 Aziz potential which is expected to give a better description of the interaction between helium atoms. The equation of state is determined with great accuracy over a wide range of densities in the liquid phase from the spinodal point up to the freezing density. The spinodal decomposition density is estimated and other properties of the liquid, such as radial distribution function, static form factor, momentum distribution and density dependence of the condensate fraction are all presented.Comment: 19 pages, RevTex 3.0, 7 figures available upon reques

Optical Imaging of Very Luminous Infrared Galaxy Systems: Photometric Properties and Late Evolution

A sample of 19 low redshift (0.03$<$z$<$0.07) very luminous infrared galaxy (VLIRG: $10^{11}L_\odot<$ L[8-1000 $\mu$m] $< 10^{12} L_\odot$) systems (30 galaxies) has been imaged in $B$, $V$, and $I$. These objects cover a luminosity range that is key to linking the most luminous infrared galaxies with the population of galaxies at large. We have obtained photometry for all of these VLIRG systems, the individual galaxies (when detached), and their nuclei, and the relative behavior of these classes has been studied in optical color-magnitude diagrams. The photometric properties of the sample are also compared with previously studied samples of ULIRGs. The mean observed photometric properties of VLIRG and ULIRG samples, considered as a whole, are indistinguishable at optical wavelengths. This suggests that not only ULIRG, but also the more numerous population of VLIRGs, have similar rest-frame optical photometric properties as the submillimeter galaxies (SMG), reinforcing the connection between low-{\it z} LIRGs -- high-{\it z} SMGs. When the nuclei of the {\it young} and {\it old} interacting systems are considered separately, some differences between the VLIRG and the ULIRG samples are found. In particular, the old VLIRGs are less luminous and redder than old ULIRG systems. If confirmed with larger samples, this behavior suggests that the late-stage evolution is different for VLIRGs and ULIRGs. Specifically, as suggested from spectroscopic data, the present photometric observations support the idea that the activity during the late phases of VLIRG evolution is dominated by starbursts, while a higher proportion of ULIRGs could evolve into a QSO type of object.Comment: 27 pages, 5 figures (degraded to reduce space). Figures 1 and 2 are multiple page figures (i.e. Fig 1a,b and Fig2a-g

Chandra Observations of Extended X-ray Emission in Arp 220

We resolve the extended X-ray emission from the prototypical ultraluminous infrared galaxy Arp 220. Extended, faint edge-brightened, soft X-ray lobes outside the optical galaxy are observed to a distance of 10 to 15 kpc on each side of the nuclear region. Bright plumes inside the optical isophotes coincide with the optical line emission and extend 11 kpc from end to end across the nucleus. The data for the plumes cannot be fit by a single temperature plasma, and display a range of temperatures from 0.2 to 1 keV. The plumes emerge from bright, diffuse circumnuclear emission in the inner 3 kpc centered on the Halpha peak, which is displaced from the radio nuclei. There is a close morphological correspondence between the Halpha and soft X-ray emission on all spatial scales. We interpret the plumes as a starburst-driven superwind, and discuss two interpretations of the emission from the lobes in the context of simulations of the merger dynamics of Arp 220.Comment: Accepted for publication in ApJ; see also astro-ph/0208477 (Paper 1

Chandra Observations of Arp 220: The Nuclear Source

We present the first results from 60ks of observations of Arp 220 using the ACIS-S instrument on Chandra. We report the detection of several sources near the galaxy's nucleus, including a point source with a hard spectrum that is coincident with the western radio nucleus B. This point source is mildly absorbed (N_H ~ 3 x 10^22 cm^-2) and has an estimated luminosity of 4 x 10^40 erg/s. In addition, a fainter source may coincide with the eastern nucleus A. Extended hard X-ray emission in the vicinity raises the total estimated nuclear 2-10 keV X-ray luminosity to 1.2 x 10^41 erg/s, but we cannot rule out a hidden AGN behind columns exceeding 5 x 10^24 cm^-2. We also detect a peak of soft X-ray emission to the west of the nucleus, and a hard point source 2.5 kpc from the nucleus with a luminosity of 6 x 10^39 erg/s.Comment: Accepted for publication in Ap