Chromosome movement in lysed mitotic cells is inhibited by vanadate.

Mitotic PtK1 cells, lysed at anaphase into a carbowax 20 M Brij 58 solution, continue to move chromosomes toward the spindle poles and to move the spindle poles apart at 50% in vivo rates for 10 min. Chromosome movements can be blocked by adding metabolic inhibitors to the lysis medium and inhibition of movement can be reversed by adding ATP to the medium. Vanadate at micromolar levels reversibly inhibits dynein ATPase activity and movement of demembranated flagella and cilia. It does not affect glycerinated myofibril contraction or myosin ATPase activty at less than millimolar concentrations. Vanadate at 10--100 micron reversibly inhibits anaphase movement of chromosomes and spindle elongation. After lysis in vanadate, spindles lose their fusiform appearance and become more barrel shaped. In vitro microtubule polymerization is insensitive to vanadate

Studies of the marine crustal magnetization at intermediate wavelengths

The data can be filtered at intermediate wavelengths to provde a data set which complements the satellite fields of MAGSAT, TSS and GRM. The filtered marine data set provides a high resolution data set which is closer to the source bodies than satellite survey data. However, the GRM and TSS could provide the necessary resolution to match the filtered sea surface field. The added resolution determines the nature of crustal magnetizations which give rise to the intermediate wavelength field. It is found that remanent magnetization is an important component over the oceans. Crustal deformation and plate motions result in magnetization vectors which differ significantly from the present day field directions. Induced magnetization or GRM are important components over the oceanic plateaus and spreading centers

Comment on "Tectonic Rotations in Extensional Regimes and Their Paleomagnetic Consequences for Ocean Basalts" by Kenneth L. Verosub and Eldridge M. Moores

One of the more intriguing results from palcomagnetic studies of Deep Sea Drilling Project (DSDP) basalts is that a surprisingly large number of samples have inclinations that deviate significantly from expected values. Vetosub and Moores [1981] sought to account for what appear to be systematic departures of mean inclination at several DSDP basement sites in terms of tectonic rotations along listtic normal faults. Such rotations, about horizontal axes perpendicular to the extension direction and typically amounting to 30ø-50 ø but as large as 70 ø to 90 ø, were suggested to be characteristic of an extensional tectonic regime such as near an ocean spreading ridge system. There is a clear implication that large tectonic rotations are a characteristic process associated with ocean crust formation

Cytological characterization and allelism testing of anther developmental mutants identified in a screen of maize male sterile lines.

Proper regulation of anther differentiation is crucial for producing functional pollen, and defects in or absence of any anther cell type result in male sterility. To deepen understanding of processes required to establish premeiotic cell fate and differentiation of somatic support cell layers a cytological screen of maize male-sterile mutants has been conducted which yielded 42 new mutants including 22 mutants with premeiotic cytological defects (increasing this class fivefold), 7 mutants with postmeiotic defects, and 13 mutants with irregular meiosis. Allelism tests with known and new mutants confirmed new alleles of four premeiotic developmental mutants, including two novel alleles of msca1 and single new alleles of ms32, ms8, and ocl4, and two alleles of the postmeiotic ms45. An allelic pair of newly described mutants was found. Premeiotic mutants are now classified into four categories: anther identity defects, abnormal anther structure, locular wall defects and premature degradation of cell layers, and/or microsporocyte collapse. The range of mutant phenotypic classes is discussed in comparison with developmental genetic investigation of anther development in rice and Arabidopsis to highlight similarities and differences between grasses and eudicots and within the grasses

Extension in the western Ross Sea region-links between Adare Basin and Victoria Land Basin

Spreading in the Adare Basin off north-western Ross Sea (43–26 Ma) and extension in the Victoria Land Basin (VLB, > 36 Ma) are used to constrain the pole of rotation for the Adare Basin, providing a rifting model for the region for the past 45 Ma. The offset from Northern Basin to VLB at about 74°S coincides with the linear Polar-3 magnetic anomaly, inferred to be caused by a major 48 - 34 Ma igneous intrusion. The style of extension apparently changed at about 34 Ma, with the end of intrusion at the Polar-3 anomaly, a change from highly asymmetric extension in Adare Basin, and the onset of major subsidence on the flanks of VLB. Ductile lower crustal and lithospheric flow is proposed as the cause of the inferred thick crust underlying southern Adare Basin, and a result of the constraining of extension to the adjacent contiguous Northern Basin

New Constraints on Plate Tectonic Puzzle of the SW Pacific

A long-standing problem in the tectonics of the southwest Pacific has been the lack of closure of the plate circuit linking the Antarctic, Australia, Lord Howe Rise, and Pacific plates in late Cretaceous and early Tertiary time [Molnar et al., 1975]. Avoiding unacceptable overlaps and underlaps in reconstructions of these plates requires invoking relative motion on one or more nebulous plate boundaries somewhere along the plate circuit, such as between East and West Antarctica, within West Antarctica [Stock and Molnar, 1987], or perhaps between the Lord Howe Rise and Challenger Plateau in the Tasman Sea [Lawver and Gahagan, 1994]. This problem is of more than mere local interest since the motion of the Pacific plate relative to the rest of the globe is constrained through its connection with West Antarctica

The role of the integral membrane nucleoporins Ndc1p and Pom152p in nuclear pore complex assembly and function

The nuclear pore complex (NPC) is a large channel that spans the two lipid bilayers of the nuclear envelope and mediates transport events between the cytoplasm and the nucleus. Only a few NPC components are transmembrane proteins, and the role of these proteins in NPC function and assembly remains poorly understood. We investigate the function of the three integral membrane nucleoporins, which are Ndc1p, Pom152p, and Pom34p, in NPC assembly and transport in Saccharomyces cerevisiae. We find that Ndc1p is important for the correct localization of nuclear transport cargoes and of components of the NPC. However, the role of Ndc1p in NPC assembly is partially redundant with Pom152p, as cells lacking both of these proteins show enhanced NPC disruption. Electron microscopy studies reveal that the absence of Ndc1p and Pom152p results in aberrant pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus

Revised Eocene-Oligocene kinematics for the West Antarctic rift system

Past plate motion between East and West Antarctica along the West Antarctic rift system had important regional and global implications. Although extensively studied, the kinematics of the rift during Eocene-Oligocene time still remains elusive. Based on a recent detailed aeromagnetic survey from the Adare and Northern Basins, located in the northwestern Ross Sea, we present the first well-constrained kinematic model with four rotations for Anomalies 12o, 13o, 16y, and 18o (26.5–40.13 Ma). These rotation poles form a cluster suggesting a stable sense of motion during that period of time. The poles are located close to the central part of the rift implying that the local motion varied from extension in the western Ross Sea sector (Adare Basin, Northern Basin, and Victoria Land Basin) to dextral transcurrent motion in the Ross Ice Shelf and to oblique convergence in the eastern end of the rift zone. The results confirm previous estimates of 95 km of extension in the Victoria Land Basin

Source of tiny wiggles in Chron C5: A comparison of sedimentary relative intensity and marine magnetic anomalies

In addition to the well-established pattern of polarity reversals, short-wavelength fluctuations are often present in both sea-surface data ( tiny wiggles ) and near-bottom anomaly data. While a high degree of correlation between different geographical regions suggests a geomagnetic origin for some of these wiggles, anomaly data alone cannot uniquely determine whether they represent short reversals or paleointensity variations. Independent evidence from another geomagnetic recording medium such as deep-sea sediments is required to determine the true nature of the tiny wiggles. We present such independent evidence in the form of sedimentary relative paleointensity from Chron C5. We make the first comparison between a sedimentary relative paleointensity record (ODP Site 887 at 54°N, 148°W) and deep-tow marine magnetic anomaly data (43°N, 131°W) [Bowers et al., 2001] for Chron C5. The sediment cores are densely sampled at ~2.5 kyr resolution. The inclination record shows no evidence for reverse intervals within the ~1 myr-long normal Chron C5n.2n. Rock magnetic measurements suggest that the primary magnetic carrier is pseudo-single domain magnetite. We choose a partial anhysteretic magnetization (pARM) as our preferred normalizer, and the resulting relative paleointensity record is used as input to a forward model of crustal magnetization. We then compare the results of this model with the stacked deep-tow anomaly records. The two records show a significant degree of correlation, suggesting that the tiny wiggles in the marine magnetic anomalies are likely produced by paleointensity variations. An analysis of our sampling density suggests that if any reverse intervals exist at this site, they are likely to be \u3c5 kyr in duration. Furthermore, we suggest that reverse intervals during Chron C5n.2n documented in other locations are unlikely to be global

A parsimonious explanation for intersecting perinatal mortality curves: understanding the effects of race and of maternal smoking

BACKGROUND: Neonatal mortality rates among black infants are lower than neonatal mortality rates among white infants at birth weights <3000 g, whereas white infants have a survival advantage at higher birth weights. This finding is also observed when birth weight-specific neonatal mortality rates are compared between infants of smokers and non-smokers. We provide a parsimonious explanation for this paradoxical phenomenon. METHODS: We used data on births in the United States in 1997 after excluding those with a birth weight <500 g or a gestational age <22 weeks. Birth weight- and gestational age-specific perinatal mortality rates were calculated per convention (using total live births at each birth weight/gestational age as the denominator) and also using the fetuses at risk of death at each gestational age. RESULTS: Perinatal mortality rates (calculated per convention) were lower among blacks than whites at lower birth weights and at preterm gestational ages, while blacks had higher mortality rates at higher birth weights and later gestational ages. With the fetuses-at-risk approach, mortality curves did not intersect; blacks had higher mortality rates at all gestational ages. Increases in birth rates and (especially) growth-restriction rates presaged gestational age-dependent increases in perinatal mortality. Similar findings were obtained in comparisons of smokers versus nonsmokers. CONCLUSIONS: Formulating perinatal risk based on the fetuses-at-risk approach solves the intersecting perinatal mortality curves paradox; blacks have higher perinatal mortality rates than whites and smokers have higher perinatal mortality rates than nonsmokers at all gestational ages and birth weights