598 research outputs found
CollodictyonâAn Ancient Lineage in the Tree of Eukaryotes
The current consensus for the eukaryote tree of life consists of several large assemblages (supergroups) that are hypothesized to describe the existing diversity. Phylogenomic analyses have shed light on the evolutionary relationships within and between supergroups as well as placed newly sequenced enigmatic species close to known lineages. Yet, a few eukaryote species remain of unknown origin and could represent key evolutionary forms for inferring ancient genomic and cellular characteristics of eukaryotes. Here, we investigate the evolutionary origin of the poorly studied protist Collodictyon (subphylum Diphyllatia) by sequencing a cDNA library as well as the 18S and 28S ribosomal DNA (rDNA) genes. Phylogenomic trees inferred from 124 genes placed Collodictyon close to the bifurcation of the âunikontâ and âbikontâ groups, either alone or as sister to the potentially contentious excavate Malawimonas. Phylogenies based on rDNA genes confirmed that Collodictyon is closely related to another genus, Diphylleia, and revealed a very low diversity in environmental DNA samples. The early and distinct origin of Collodictyon suggests that it constitutes a new lineage in the global eukaryote phylogeny. Collodictyon shares cellular characteristics with Excavata and Amoebozoa, such as ventral feeding groove supported by microtubular structures and the ability to form thin and broad pseudopods. These may therefore be ancient morphological features among eukaryotes. Overall, this shows that Collodictyon is a key lineage to understand early eukaryote evolution
Recommended from our members
Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection
In this paper, a novel method for interaction detection is presented to compare the contact dynamics of macrophages in the Drosophila embryo. The study is carried out by a framework called macrosight, which analyses the movement and interaction of migrating macrophages. The framework incorporates a segmentation and tracking algorithm into analysing the motion characteristics of cells after contact. In this particular study, the interactions between cells is characterised in the case of control embryos and Shot mutants, a candidate protein that is hypothesised to regulate contact dynamics between migrating cells. Statistical significance between control and mutant cells was found when comparing the direction of motion after contact in specific conditions. Such discoveries provide insights for future developments in combining biological experiments with computational analysis
Population analysis of open clusters: radii and mass segregation
Aims: Based on our well-determined sample of open clusters in the all-sky
catalogue ASCC-2.5 we derive new linear sizes of some 600 clusters, and
investigate the effect of mass segregation of stars in open clusters. Methods:
Using statistical methods, we study the distribution of linear sizes as a
function of spatial position and cluster age. We also examine statistically the
distribution of stars of different masses within clusters as a function of the
cluster age. Results: No significant dependence of the cluster size on location
in the Galaxy is detected for younger clusters (< 200 Myr), whereas older
clusters inside the solar orbit turned out to be, on average, smaller than
outside. Also, small old clusters are preferentially found close to the
Galactic plane, whereas larger ones more frequently live farther away from the
plane and at larger Galactocentric distances. For clusters with (V - M_V) <
10.5, a clear dependence of the apparent radius on age has been detected: the
cluster radii decrease by a factor of about 2 from an age of 10 Myr to an age
of 1 Gyr. A detailed analysis shows that this observed effect can be explained
by mass segregation and does not necessarily reflect a real decrease of cluster
radii. We found evidence for the latter for the majority of clusters older than
30 Myr. Among the youngest clusters (between 5 and 30 Myr), there are some
clusters with a significant grade of mass segregation, whereas some others show
no segregation at all. At a cluster age between 50 and 100 Myrs, the
distribution of stars of different masses becomes more regular over cluster
area. In older clusters the evolution of the massive stars is the most
prominent effect we observe.Comment: 14 pages, 12 figures, accepted for publication in Astronomy &
Astrophysic
Velocity Dispersion of Dissolving OB Associations Affected by External Pressure of Formation Environment
This paper presents a possible way to understand dissolution of OB
associations (or groups). Assuming rapid escape of parental cloud gas from
associations, we show that the shadow of the formation environment for
associations can be partially imprinted on the velocity dispersion at their
dissolution. This conclusion is not surprising as long as associations are
formed in a multiphase interstellar medium, because the external pressure
should suppress expansion caused by the internal motion of the parental clouds.
Our model predicts a few km s as the internal velocity dispersion.
Observationally, the internal velocity dispersion is km s which
is smaller than our prediction. This suggests that the dissipation of internal
energy happens before the formation of OB associations.Comment: 6 pages. AJ accepte
Envelope Ejection: an Alternative Process for some Early Case B Binaries
We discuss the evolution of binaries with moderately high masses (about 10 -
30 solar masses), and with periods of about 3 - 300d, corresponding mostly to
early Case B. These are usually thought to evolve either by reasonably
conservative Roche-lobe overflow, if the initial mass ratio is fairly mild, or
else by highly non-conservative common-envelope evolution, with spiral-in to
short periods (hours, typically), if the initial mass ratio is rather extreme.
We discuss here a handful of binaries from part of this period range (about 50
- 250d), which appear to have followed a different path: we argue that they
must have lost a large proportion of initial mass (about 70 - 80%), but without
shortening their periods at all. We suggest that their behaviour may be due to
the fact that stars of such masses, when evolved also to rather large radii,
are not far from the Humphreys-Davidson limit where single stars lose their
envelopes spontaneously in P Cygni winds, and so have envelopes which are only
lightly bound to the core. These envelopes therefore may be relatively easily
dissipated by the perturbing effect of a companion. In addition, some or all of
the stars considered here may have been close to the Cepheid instability strip
when they filled their Roche lobes. One or other, or both, of high luminosity
and Cepheid instability, in combination with an appropriately close binary
companion, may be implicated
The MACHO Project LMC Variable Star Inventory. VI. The Second-overtone Mode of Cepheid Pulsation From First/Second Overtone (FO/SO) Beat Cepheids
MACHO Project photometry of 45 LMC FO/SO beat Cepheids which pulsate in the
first and second overtone (FO and SOo, respectively) has been analysed to
determine the lightcurve characteristics for the SO mode of Cepheid pulsation.
We predict that singly-periodic SO Cepheids will have nearly sinusoidal
lightcurves; that we will only be able to discern SO Cepheids from fundamental
(F) and (FO) Cepheids for P <= 1.4 days; and that the SO distribution will
overlap the short-period edge of the LMC FO Cepheid period-luminosity relation
(when both are plotted as a function of photometric period).
We also report the discovery of one SO Cepheid candidate,
MACHO*05:03:39.670:04:32, with a photometric period of 0.775961 +/- 0.000019
days and an instrumental amplitude of 0.047 +/- 0.009 mag in V.Comment: 23 pages, 7 Encapsulated PostScript figures. Accepted for publication
in the Astrophysical Journa
Functional significance may underlie the taxonomic utility of single amino acid substitutions in conserved proteins
We hypothesized that some amino acid substitutions in conserved proteins that are strongly fixed by critical functional roles would show lineage-specific distributions. As an example of an archetypal conserved eukaryotic protein we considered the active site of Ă-tubulin. Our analysis identified one amino acid substitutionâĂ-tubulin F224âwhich was highly lineage specific. Investigation of Ă-tubulin for other phylogenetically restricted amino acids identified several with apparent specificity for well-defined phylogenetic groups. Intriguingly, none showed specificity for âsupergroupsâ other than the unikonts. To understand why, we analysed the Ă-tubulin Neighbor-Net and demonstrated a fundamental division between core Ă-tubulins (plant-like) and divergent Ă-tubulins (animal and fungal). F224 was almost completely restricted to the core Ă-tubulins, while divergent Ă-tubulins possessed Y224. Thus, our specific example offers insight into the restrictions associated with the co-evolution of Ă-tubulin during the radiation of eukaryotes, underlining a fundamental dichotomy between F-type, core Ă-tubulins and Y-type, divergent Ă-tubulins. More broadly our study provides proof of principle for the taxonomic utility of critical amino acids in the active sites of conserved proteins
Discovery of a peculiar Cepheid-like star towards the northern edge of the Small Magellanic Cloud
For seven years, the EROS-2 project obtained a mass of photometric data on
variable stars. We present a peculiar Cepheid-like star, in the direction of
the Small Magellanic Cloud, which demonstrates unusual photometric behaviour
over a short time interval. We report on data of the photometry acquired by the
MARLY telescope and spectroscopy from the EFOSC instrument for this star,
called EROS2 J005135-714459(sm0060n13842), which resembles the unusual Cepheid
HR 7308. The light curve of our target is analysed using the Analysis of
Variance method to determine a pulsational period of 5.5675 days. A fit of
time-dependent Fourier coefficients is performed and a search for proper motion
is conducted. The light curve exhibits a previously unobserved and spectacular
change in both mean magnitude and amplitude, which has no clear theoretical
explanation. Our analysis of the spectrum implies a radial velocity of 104 km
s and a metallicity of -0.40.2 dex. In the direction of right
ascension, we measure a proper motion of 17.46.0 mas yr using EROS
astrometry, which is compatible with data from the NOMAD catalogue. The nature
of EROS2 J005135-714459(sm0060n13842) remains unclear. For this star, we may
have detected a non-zero proper motion for this star, which would imply that it
is a foreground object. Its radial velocity, pulsational characteristics, and
photometric data, however, suggest that it is instead a Cepheid-like object
located in the SMC. In such a case, it would present a challenge to
conventional Cepheid models.Comment: Correction of typos in the abstrac
- âŠ