Improved liquid chromatographic method for determination of carotenoids in carrot

Carotenoids are a large class of plant metabolites with a function of either essential nutrients or health promoting compounds for humans. Carrot root is a well-known and significant source of dietary carotenoids, mainly: α- and β-carotene, lutein and lycopene. These pigments are the main carotenoids separated and quantified routinely by HPLC analysis. However, little is known about minor carotenoids, carotenoid esters and the carotenoids present in leaves despite their potential interest in metabolic and physiological studies. Previous works used C-18 columns but these stationary phases provide a poor resolution of structurally similar compounds and geometrical isomers. In recent years, C-30 columns have been developed and successfully applied at the separation of carotenoids from various plant materials, the number of resolved carotenoids being significantly improved. Based on literature procedures, we have developed a HPLC-DAD method with a C-30 column, adapted to the quantification of carotenoid compounds from carrot roots and leaves. A simple and rapid extraction method was optimized for both these types of samples on a panel of 5 genotypes displaying distinct root colours (different carotenoid composition and contents). Carotenoids from roots were separated in 23 minutes while carotenoids and chlorophylls from leaves were separated in 42 minutes. Compounds were identified according to their retention time and UV-visible spectrum in comparison with authentic standards (analysed individually and in combination, in the same conditions), or with data from literature, when standards were unavailable. Results showed that carrot root exhibited a simple profile with only 1 to 3 main carotenoids whereas a more complex composition was noticed in leaves, containing both identified and unidentified carotenoids and chlorophylls. Moreover, the composition was quite conservative for leaves but depended on the genotype for roots

Facteurs predictifs d’atteinte ganglionnaire centrale dans les cancers differencies de la thyroïde. Analyses univariée et multivariée

Les cancers papillaires et vésiculaires de la thyroïde sont les cancers endocriniens les plus fréquents. L’envahissement ganglionnaire semble peu influencer la survie globale, mais majore les risques de récidive locorégionale et de métastases à distance. Cet envahissement est une complication fréquente des cancers papillaires de la thyroïde, moins rapporté dans les cancers vésiculaires. il implique un évidement ganglionnaire qui n’est pas sans risques. Pour déterminer les moyens de limiter les curages ganglionnaires, nous avons tenté de déterminer les critères per opératoires prédictifs de métastase ganglionnaire centrale et ainsi identifier les patients qui doivent bénéficier de cette procédure. Notre étude est une étude rétrospective portant sur 71 patients (49 femmes et 22 hommes) traités entre 2000 et 2011d’un cancer différencié de la thyroïde de type papillaire ou vésiculaire. Après analyse des critères épidémiologiques, cliniques et anatomopathologiques, nous avons trouvé une association significative entre l’envahissement extra capsulaire, l’envahissement ganglionnaire latéral, la bilatéralité et l’envahissement ganglionnaire central. un évidemment ganglionnaire central prophylactique doit donc être particulièrement considéré chez ces patients.Mots clés : carcinome thyroïdien, évidement ganglionnaire, métastase ganglionnaire.Papillary and follicular thyroid carcinomas are the most common form of endocrine carcinomas. Lymph node involvement seems to be a low risk factor for death, but it increases the risk for loco-regional recurrences and distant metastasis. Lymph node involvement is a common complication of papillary thyroid carcinoma, less reported in vesicular carcinoma. il involves node dissection and its resulting morbidity. To determine means of limiting lymph node dissections, we attempted to determine intra-operative criteria predictive of node metastasis and soidentify the patients likely to benefit from this procedure. Our study is a retrospective study of 71 patients (49 women and 22 men) treated between 2000 and 2011 from differentiated thyroid cancer (papillary or follicular).After analyses of the demographic, clinical and anatomopathological factors, we found a significant association among extra capsular invasion, lateral lymphnode metastasis, bilaterality, and central compartment lymph node metastasis. A prophylactic neck dissection of the central compartment should be considered particularly in these patients.Keyswords : thyroid carcinoma, lymph node dissection, lymph node metastases

Evaluation des facteurs pronostiques pouvant influencer les resultats d’une septoplastie

Introduction :Une des principales étiologies de l’obstruction nasale est la déviation septale, dont le traitement chirurgical est la septoplastie. mais d’autres étiologies peuvent être à l’origine d’une obstruction nasale, notamment une hypertrophie des cornets, des végétations adénoïdes hypertrophiques, une polypose nasale, etc. Les critères de sélection sur lesquels se base un chirurgien pour décider de pratiquer une septoplastie, sont essentiellement basés sur l’évaluation clinique. Le but de nôtre travail est d’évaluer ces critères cliniques ainsi que les critères dermographiques pouvant influencer les résultats de la septoplastie.Patients et méthodes : On a évalué tous les patients qui ont eu une septoplastie dans les 2 à 10 dernières années. Les données cliniques ont été revues afin de préciser le siège de la déformation septale initiale, sa sévérité dictée par le chirurgien et les résultats fonctionnels de la septoplastie.Résultats et : les patients ayant une déviation septale antérieure ont rapporté le plus d’amélioration après septoplastie. Toutefois il ressort de notre étude qu’une sélection des patients basée uniquement sur l’évaluation clinique par le chirurgien de l’importance de la déviation septale est à l’origine d’un taux important d’insatisfaction des patientsObjective : a common etiology of nasal obstruction is deviation of the nasal septum, for witch septoplasty is the definitive treatment. But nasal obstruction can be caused by other conditions, such as turbinate hypertrophy, adenoid hypertrophy, and nasal polyposis. The surgeons’ selection criteria for septoplasty largely rely on clinical judgment alone. The aim of our study was to evaluate if this clinical criteria and the demographics ones could influence the results of septoplasty.Patients and methods :We evaluate all patients who underwent septoplasty in the past 2 to 10 years. Clinical files were reviewed to establish the initial type of septal deformity, its severity dictated by the surgeon and the functional results of septoplasty.Results and Conclusion : Subjects with anterior septal deformities were shown to benefit the most from septoplasty. However, selection of patients based on clinical evaluation alone does carry a considerable risk of patient dissatisfaction with end results

Les cellulites cervico-faciales d’origine dentaire: a propos de 150 cas

Les cellulites cervico-faciales d’origine dentaire sont des infections des tissus cellulo-adipeux, de la face et du cou, ayant des potentialités extensives pouvant parfois être graves et engager le pronostic vital. Le but de ce travail est d’étudier le profil épidémioclinique et paraclinique de nos patients, d’évaluer leur prise en charge thérapeutique et leur évolution. Notre étude est rétrospective et a porté sur 150 cas de cellulites cervico-faciales d’origine dentaire colligés sur 10 ans entre 1997 et 2006. La prise en charge thérapeutique était médico-hirurgicale. Les cellulites cervico-faciales d’origine dentaire sont une pathologie potentiellement grave dont la prise en charge est coûteuse et les répercussions socio-professionnelles et économiques sont lourdes d’où l’intérêt d’une politique de prévention efficace et ciblée visant à réduire la morbidité liée à ces infections.Mots clés : cellulites cervicale et faciale, infections dentaire, prévention

Carcinome hybride de la fosse nasale

Hybrid carcinoma is a rare neoplasm, accounting for less than 0.1% of all registered tumors in salivary glands. Up to now, only one case of hybrid carcinoma of the nasal cavity has been described. In this report, we describe a case of hybrid carcinoma composed of epithelial-myoepithelial carcinoma with an adenoid cystic carcinoma component occurring in the nasal cavity in a 56-year-old woman. Caldwell-Luc means resection was performedand the patient revisited because of the tumor recurrence within two years of evolution. A second surgery was accomplished by means of Red Denker and a adjuvant radiation therapy is envisaged. We have not enough detachmentto speak about actual evolution. It is in our knowledge the second case of hybrid tumor of the nasal cavity described in the literature. Hybrid carcinoma of the nasal cavity is possible although rare. Therefore recognition of other component that is more aggressive and has a worse prognosis has therapeutic and prognostic ramifications.Keywords: Carcinoma; hybrid; tumors of nasal cavity

La baisse de la densité osseuse au cours des maladies inflammatoires chroniques de l’intestin : prévalence et facteurs de risqué

Introduction: La baisse de la densité minérale osseuse représente la principale manifestation osseuse décrite au cours des maladies inflammatoires chroniques de l'intestin. En Tunisie, très peu d'études ont rapportés sa prévalence et ses facteurs de risque. Le but de ce travail était de déterminer la prévalence de la perte osseuse au cours des maladies inflammatoires chroniques de l'intestin, et rechercher ses facteurs de risque. Méthodes: Patients et méthodes: étude ouverte transversale, réalisée de 2007 jusqu'à 2012. Résultats: 146 cas étaient colligés, dont 105 avaient une maladie de Crohn (71,9%) et 41 avaient une rectocolite hémorragique (28,1%). Il s'agissait de 62 hommes et 84 femmes. L'âge moyen était de 33,18 ans. La perte osseuse était trouvée chez 85 patients (58,2%). Il s'agissait d'une ostéopénie dans 57 cas et d'ostéoporose dans 28 cas. Les facteurs de risque de perte osseuse étaient une activité physique limitée  (p=0,013), un indice de masse corporel '20 kg/m2 (p=0,015), une maladie active (p=0,035), l'étendue de l'atteinte intestinale (p=0,006) et une dose cumulée de corticothérapie dépassant 4,5g de Prednisone (p=0,003). Conclusion: La déminéralisation osseuse est une complication fréquente mais non constante. Ceci justifie un dépistage précoce chez les patients à risque, qui pourront ainsi bénéficier d'un traitement substitutif.Key words: Maladie de Crohn, recto-colite hémorragique, densité minérale osseuse, ostéopénie, ostéoporos

Osteomyelite atypique ou centrale de la base du crane d’origine sinusienne

Introduction : L’ostéomyélitede la base du crâne esttypiquement secondaire à une otite externe nécrosante chez des patients âgés, diabétiques. Elle atteint l’os temporal et est habituellement due au pseudomonasaeruginosa. Dans sa forme atypique, l’ostéomyélite atteint les os sphénoïdal ou occipital sans otite externe associéeet est beaucoup moins fréquente. Le but de cette étude est d’analyser les caractéristiques cliniques et de déterminer les modalités thérapeutiques de l’ostéomyélite nonotologique de la base du crâne.Méthodes : Nous avons revu rétrospectivement deux casd’ostéomyélite atypique de la base du crâne.Résultats : Les deux patients se sont présentés dans un tableau d’altération de l’état général, une asthénie, des céphalées et de la fièvre. Les signes rhinologiques étaient discrets. Aucun des deux patients n’avait de déficit neurologique. L’imagerie avait montré une destruction osseuse, la biologie a objectivé un syndrome infectieux et une biopsie osseuse trans sphénoïdale a confirmé le diagnostic d’ostéomyélite. Un traitement antibiotique prolongé a été instauré et les deux patients ont été guéris sans récidive moyennant un recul de 12 mois.Conclusion : l’atteinte ostéomyélitique centrale de la base du crâne est une affection rare dont la prise en charge passe par un prélèvement osseux précoce et un traitement approprié afin de prévenir ou limiter d’autres complications telles qu’une extension endocrânienne, un empyème, ou la mort.Mots Clés: Ostéomyélite; Base du crâne; sphenoïdite; IRMIntroduction : Skull base osteomyelitis typically arises as a complication of ear infection in older diabetic patients, involves the temporal bone, and has Pseudomonas aeruginosaas the usual pathogen. Atypical skull base osteomyelitis arising from the sphenoid or occipital bones without associated external otitis occurs much less frequently. The purpose of this study was to review the clinical featuresand determine efficacy of treatment for nonotologic osteomyelitis of the skull base.Methods : We retrospectively reviewed two cases of central or atypical skull base osteomyelitis.Results : The two patients presented with asthenia, headache, and fever. No patient presented with neurologic deficits. Contrary to malignant otitis externa, our cases were not preceded by immediate external infections and had normal external ear examinations. One patient had an underlying immunocompromising condition. Imaging demonstrated bone destruction, subsequent microbiological analysis diagnosed infection and a biopsy proved diagnosis of osteomyelitis, a prolonged antibiotic treatment was instored. The two patients were cured with no recurrence of skull base infection over 12-month follow-up period.Conclusion : Central skull base osteomyelitis is a rare disease whose treatment requires an early bone biopsy and appropriate treatment to prevent or limit other complications such as intracranial extension, empyema, or dead.Key Words : Osteomyelitis; Skull base; sphenoïditis; MR

Differential Pigment Accumulation in Carrot Leaves and Roots during Two Growing Periods

Carotenoids are important secondary metabolites involved in plant growth and nutritional quality of vegetable crops. These pigments are highly accumulated in carrot root but the knowledge about the environmental factors on their accumulation is limited. The purpose of this work was to investigate the impact of environmental variations on carotenoid accumulation in carrot leaves and roots. In this work, carrots were grown during two contrasted periods to maximize bioclimatic differences. In leaves, carotenoid and chlorophyll contents were lower in the less favorable growing condition while relative contents were well conserved for all genotypes suggesting a common regulatory mechanism. The down-regulation of all genes under environmental constraints demonstrates that carotenoid accumulation is regulated at transcriptional level. In roots, the decrease in α-carotene and lutein contents was accompanied by an increase of β-carotene relative content. At transcriptional level, LCYB and ZEP expression increased while LCYE expression decreased in the less favorable condition, suggesting that carotenoid biosynthesis is switched toward the β-branch

Hopping conduction mechanism and impedance spectroscopy analyses of La0.70Sr0.25Na0.05Mn0.70Ti0.30O3 ceramic

The perovskite sample La0.7Sr0.25Na0.05Mn0.7Ti0.3O3 (LSNM0.70T0.30) was produced via a solid-state route process. The frequency dependence of electrical conduction plot established that according to the Jonscher law. The electrical conduction process was based on both theoretical conduction models assigned to the non-overlapping small polaron tunneling model at low temperatures and correlated barrier hopping mechanism at high temperatures. Detailed investigation of impedance data revealed a non-Debye-type relaxation occurring in the polycrystalline. In addition, the dielectric response confirmed the dominance of the Maxwell–Wagner model and Koop’s phenomenological theory effect in conduction phenomenon. The values of permittivity is high for LSNM0.70T0.30 were observed. These values make this composition interesting for microelectric applications. In the thermal study, the relaxation processes observed by electrical conductivity, impedance, and modulus are associated with singly and doubly ionized oxygen vacancies for the lower and higher temperature, respectively. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.Majmaah University, MU: R-2021-121The author would like to thank Deanship of Scientific Research at Majmaah University for supporting this work under Project Number No. R-2021-121

Factors to Consider During Identification and Invitation of Individuals in a Multi-stakeholder Research Partnership

Background: Health research teams increasingly partner with stakeholders to produce research that is relevant, accessible, and widely used. Previous work has covered stakeholder group identification. Objective: We aimed to develop factors for health research teams to consider during identification and invitation of individual representatives in a multi-stakeholder research partnership, with the aim of forming equitable and informed teams. Design: Consensus development. Participants: We involved 16 stakeholders from the international Multi-Stakeholder Engagement (MuSE) Consortium, including patients and the public, providers, payers of health services/purchasers, policy makers, programme managers, peer review editors, and principal investigators. Approach: We engaged stakeholders in factor development and as co-authors of this manuscript. Using a modified Delphi approach, we gathered stakeholder views concerning a preliminary list of 18 factors. Over two feedback rounds, using qualitative and quantitative analysis, we concentrated these into ten factors. Key Results: We present seven highly desirable factors: ‘expertise or experience’, ‘ability and willingness to represent the stakeholder group’, ‘inclusivity (equity, diversity and intersectionality)’, ‘communication skills’, ‘commitment and time capacity’, ‘financial and non-financial relationships and activities, and conflict of interest’, ‘training support and funding needs’. Additionally, three factors are desirable: ‘influence’, ‘research relevant values’, ‘previous stakeholder engagement’. Conclusions: We present factors for research teams to consider during identification and invitation of individual representatives in a multi-stakeholder research partnership. Policy makers and guideline developers may benefit from considering the factors in stakeholder identification and invitation. Research funders may consider stipulating consideration of the factors in funding applications. We outline how these factors can be implemented and exemplify how their use has the potential to improve the quality and relevancy of health research