Desenvolvimento e aplicação da versão em português do instrumento de avaliação de qualidade de vida da Organização Mundial da Saúde (WHOQOL-100)

OBJECTIVE: The World Health Organization developed an instrument to assess quality of life in the framework of a collaborative multicentric project. The methodology and the different stages of developing the original instrument are described. Next, the developing of the portuguese version is presented as well as its application in 300 patients in Porto Alegre, south Brazil.MATERIALS AND METHODS: Through a singular methodology, translation, discussionin focal groups with community members, patients and health professionals was performed followed by back translation.RESULTS: The instrument showed a good psychometric performance with good internal consistency, discriminant validity, criterion validity, concurrent validity and reliability.CONCLUSIONS: The authors concluded showing that the instrument is ready to be used in Brazil, being important to evaluate its performance in other regions and different samples.OBJETIVO: A Organização Mundial da Saúde desenvolveu um instrumento para avaliação de qualidade de vida através de um projeto colaborativo multicêntrico. São descritas a metodologia e as diferentes etapas de desenvolvimento do instrumentooriginal. A seguir é apresentado o desenvolvimento da versão em português e a aplicação deste em uma amostra de 300 indivíduos na cidade de Porto Alegre, sul do Brasil.MATERIAIS E MÉTODOS: Através de uma metodologia própria foi realizada a tradução, discussão em grupos focais com membros da comunidade, pacientes eprofissionais de saúde seguida de retrotradução.RESULTADOS: O Instrumento mostrou um bom desempenho psicométrico com características satisfatórias de consistência interna, validade discriminante, validade de critério, validade concorrente e fidedignidade teste-reteste.CONCLUSÕES: Os autores concluem que o instrumento está em condições de ser usado no Brasil, sendo importante avaliar seu desempenho em outras regiões e em diferentes amostras de indivíduos

EsportivaMente: Introdução à Psicologia do Esporte em uma Academia de Taekwondo em Porto Alegre

O Projeto de Extensão EsportivaMente é uma ação do grupo PET (Programa de Educação Tutorial) do curso de Psicologia da Escola de Ciências da Saúde da PUCRS (Pontifícia Universidade Católica do Rio Grande do Sul), em parceria com a Academia Oriente de Taekwondo, ambos situados na cidade de Porto Alegre, RS. O projeto teve início em 2017 orientado pelo Prof. Ângelo Brandelli Costa, tutor do grupo PET, com a supervisão de Sérgio Moller Júnior, Educador Físico e professor na Academia Oriente. O projeto busca inserir a Psicologia do Esporte no contexto da prática esportiva enquanto lazer, por meio de encontros semanais com práticas de integração ministradas pelo bolsista Tiago Soares do Canto

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione Reductase

The equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated. Unfolding was monitored by a variety of techniques, including intrinsic fluorescence emission, anisotropy and iodide quenching measurements, far-ultraviolet circular dichroism and thiol reactivity measurements. At 1 M GdnHCl, one thiol group of GR became accessible to modification with 5,5′-dithiobis-(2-nitrobenzoic) acid (DTNB), whereas no changes could be detected in the spectroscopic properties (fluorescence, circular dichroism) of the protein. Between 2 and 3 M GdnHCl, two partially folded intermediate states possessing flexible tertiary structures (revealed by fluorescence data) but compact secondary structures (as indicated by circular dichroism measurements) were identified. The quaternary structure of GR in the presence of GdnHCl was also investigated by size-exclusion liquid chromatography. These results indicated the presence of an expanded predissociated dimer at 2.5 M GdnHCl and partially folded monomers at 3 M GdnHCl. Taken together, these results suggest the existence of two molten-globule-like intermediate species (one dimeric and one monomeric) in the unfolding of GR. The results are discussed in terms of the mechanism of GR folding and dimerization

Desenvolvimento e aplicação da versão em português do instrumento de avaliação de qualidade de vida da Organização Mundial da Saúde (WHOQOL-100)

OBJECTIVE: The World Health Organization developed an instrument to assess quality of life in the framework of a collaborative multicentric project. The methodology and the different stages of developing the original instrument are described. Next, the developing of the portuguese version is presented as well as its application in 300 patients in Porto Alegre, south Brazil.MATERIALS AND METHODS: Through a singular methodology, translation, discussionin focal groups with community members, patients and health professionals was performed followed by back translation.RESULTS: The instrument showed a good psychometric performance with good internal consistency, discriminant validity, criterion validity, concurrent validity and reliability.CONCLUSIONS: The authors concluded showing that the instrument is ready to be used in Brazil, being important to evaluate its performance in other regions and different samples.OBJETIVO: A Organização Mundial da Saúde desenvolveu um instrumento para avaliação de qualidade de vida através de um projeto colaborativo multicêntrico. São descritas a metodologia e as diferentes etapas de desenvolvimento do instrumentooriginal. A seguir é apresentado o desenvolvimento da versão em português e a aplicação deste em uma amostra de 300 indivíduos na cidade de Porto Alegre, sul do Brasil.MATERIAIS E MÉTODOS: Através de uma metodologia própria foi realizada a tradução, discussão em grupos focais com membros da comunidade, pacientes eprofissionais de saúde seguida de retrotradução.RESULTADOS: O Instrumento mostrou um bom desempenho psicométrico com características satisfatórias de consistência interna, validade discriminante, validade de critério, validade concorrente e fidedignidade teste-reteste.CONCLUSÕES: Os autores concluem que o instrumento está em condições de ser usado no Brasil, sendo importante avaliar seu desempenho em outras regiões e em diferentes amostras de indivíduos