DigiWeb - a workflow environment for quality assurance of transcription in digitization of natural history collections

Data produced by digitization increases the scientific use of natural history collections. However, in mass digitization, attention must be paid to the flawless management of the workflows, and high quantities of end results should not be compromised by a low standard of quality. A web-based environment DigiWeb was created for controlling the workflow of transcribing data from images of natural history specimens. Using DigiWeb, it was possible to manage the workflow of transcription and data proofing, include all participants to the workflow, allow collaboration and training, and also to provide useful processing features. The data emerging from this process passes quality control standards which are supported by DigiWeb and based on the strict requirements of the ISO 2859 standard

One tissue, two fates: different roles of megagametophyte cells during Scots pine embryogenesis

In the Scots pine (Pinus sylvestris L.) seed, embryos grow and develop within the corrosion cavity of the megagametophyte, a maternally derived haploid tissue, which houses the majority of the storage reserves of the seed. In the present study, histochemical methods and quantification of the expression levels of the programmed cell death (PCD) and DNA repair processes related genes (MCA, TAT-D, RAD51, KU80, and LIG) were used to investigate the physiological events occurring in the megagametophyte tissue during embryo development. It was found that the megagametophyte was viable from the early phases of embryo development until the early germination of mature seeds. However, the megagametophyte cells in the narrow embryo surrounding region (ESR) were destroyed by cell death with morphologically necrotic features. Their cell wall, plasma membrane, and nuclear envelope broke down with the release of cell debris and nucleic acids into the corrosion cavity. The occurrence of necrotic-like cell death in gymnosperm embryogenesis provides a favourable model for the study of developmental cell death with necrotic-like morphology and suggests that the mechanism underlying necrotic cell death is evolutionary conserved