CTLA4 +49AG (rs231775) and CT60 (rs3087243) gene variants are not associated with alopecia areata in a Mexican population from Monterrey Mexico

Background: Alopecia areata is an autoimmune disease that produces non-scarring hair loss around the body. Gene variants of the cytotoxic T-lymphocyte antigen 4 (CTLA4) gene, a negative regulator of T-cell response, have been associated with a predisposition to autoimmune diseases in different populations; however, the involvement of these genetic variants in the development of AA is controversial. Objective: The present study evaluated the potential association of two CTLA4 gene variants with alopecia areata in a Mexican population. Methods: We genotyped +49AG (rs231775) and CT60 (rs3087243) variants in 50 AA patients and 100 healthy control participants through PCR-RFLP. Results: No statistical difference was observed for either of the gene variants regarding allele or genotype frequencies between AA patients and the controls when the parameters of family/personal history of autoimmune diseases or gender were considered (p > 0.05).Study limitations: Small sample size of patients and the data were obtained from NortheastMexico population.Conclusion: The genetic variants rs231775 and rs3087243 of the CTLA4 gene are not a risk factorfor the development of alopecia areata in the analyzed Mexican populatio

Evaluation of skin expression profiles of patients with vitiligo treated with narrow-band UVB therapy by targeted RNA-seq

Abstract: Background: Vitiligo is characterized by a lack of pigmentation in the skin. To date, there are no studies that analyze the changes in gene expression in the skin of vitiligo patients in response to narrow-band ultraviolet B (nb-UVB) phototherapy treatment. oBjective: Explore the usefulness of new generation RNA sequencing in the identification of gene expression changes in the skin of vitiligo patients treated with nb-UVB phototherapy. Methods: Four skin biopsies (4mm in diameter) were collected from 45 Mexican vitiligo vulgaris patients, 2 specimens before and 2 after treatment with nb-UVB phototherapy, obtained from pigmented and non-pigmented tissue. RNA extracted from the biopsies was analyzed using the Illumina TruSeq Targeted RNA Expression protocol to study the expression of genes that participate in pathways of skin homeostasis. The 2 groups were compared using Student’s t-test and the Mann-Whitney U-test. results: The expression analysis identified differences in 12 genes included in this study after comparing the samples obtained before and after treatment: 5 genes involved in skin pigmentation, 2 genes involved in apoptosis, 2 genes involved in cell survival, 2 genes involved in oxidative stress responses and 1 gene involved in signal transduction mechanisms (p<0.05). study liMitations: The small size of skin biopsies limits the amount of RNA obtained, the number of genes to be analyzed and the use of conventional techniques such as RT-qPCR. conclusion: We demonstrated usefulness of new generation RNA sequencing in the identification of gene expression changes, in addition to identifying new targets in the study of vitiligo. Keywords: Gene expression; Phototherapy; Vitilig

Diseño y construcción de soporte estructural para un módulo de entrenamiento

El continuo desarrollo tecnológico y su influencia en la sociedad e industria ha llevado a que los procesos de medición de variables como temperatura, presión, caudal y nivel implementen herramientas o dispositivos que permitan una mayor eficiencia, veracidad, y fácil manejo. También hace necesario que el personal que se encarga de la instalación y manejo de estos sensores tenga conocimiento adecuado que le permita comprender de manera apropiada la manipulación de estos. No obstante así, se observa con preocupación que no todos los centros de educación superior en la faculta de ingeniería electrónica van a la par con este desarrollo tecnológico, dado que no cuenta con laboratorios que faciliten la practica con sensores industriales de temperatura, presión, caudal y nivel, especialmente en un sistema organizado que requiera instalación, control y mantenimiento. Limitando de esta manera la comprensión del funcionamiento de ellos en campo real, la identificación de las diferencias entre procesos de simulación y procesos de medición en un montaje concreto, y sobre todo la posibilidad de fortalecer conocimientos teóricos en espacios aplicados.INTRODUCCIÓN 2 1. DISEÑO Y CONSTRUCCIÓN ESTRUCTURAL PARA UN MÓDULO DE ENTRENAMIENTO 3 1.1 PLANTEAMIENTO DEL PROBLEMA 3 1.2 FORMULACIÓN DEL PROBLEMA JUSTIFICACIÓN 3. OBJETIVOS 5 3.1 GENERAL 3.2 ESPECÍFICOS METODOLOGIA 4.1 CONSIDERACIONES PARA EL DISEÑO DE LA ESTRUCTURA. DIMENSIONAMIENTO DE REQUISITOS DE SEGURIDAD. FASES DEL DISEÑO IDENTIFICACIÓN DE REQUERIMIENTOS ESTRUCTURALES RESISTENCIA Y ESTABILIDAD PINTURA SOPORTES UBICACIÓN DE SOPORTES EN LA ESTRUCTURA CALCULOS REQUERIDOS PARA LA ESTRUCTURA TANQUES 4.5.2 CAPACIDAD TANQUES 4.5.3 CARACTERÍSTICAS TANQUES 4.6 ENSAMBLE DE LA ESTRUCTURA 4.6.1 SOPORTES DE APOYO 4.6.2 BOCETO DE SISTEMA HIDRÁULICO 4.6.3 DISEÑO FINAL 4.7 CONSTRUCCIÓN MANUAL DE ENSAMBLE 5. ANALISIS DE RESULTADOS 5.1 REQUERIMIENTOS ESTRUCTURALES DE RESISTENCIA 5.1.1 ESTRUCTURA METÁLICA 5.1.2 SOPORTES MANUAL DE ENSAMBLE 6. CONCLUSIONES RECOMENDACIONES TRABAJO A FUTURO ANEXOS MEMORIAS DE DISEÑO MECCI MANUAL DE ENSAMBLEPregradoIngeniero en ElectrónicaIngeniería Electrónic

Uridine 5'‑diphospho‑glucronosyltrasferase: Its role in pharmacogenomics and human disease (Review)

Abstract. Biotransformation is an enzyme-catalyzed process in which the body converts endogenous compounds, xenobiotics and toxic substances into harmless or easily excreted metabolites. The biotransformation reactions are classified as phase I and II reactions. Uridine 5'-diphospho (UDP)-glucuronosyltransferases (UGTs) are a superfamily of phase II enzymes which have roles in the conjugation of xenobiotics or endogenous compounds, including drugs and bilirubin, with glucuronic acid to make them easier to excrete. The method the human body uses to achieve glucuronidation may be affected by a large interindividual variation due to changes in the sequences of the genes encoding these enzymes. In the last five years, the study of the genetic variants of the UGTs at a molecular level has become important due to its association with several diseases and the ability to predict adverse events due to drug metabolism. In the present review, the structure and the prominent genetic variants of the UGT1A subfamily and their metabolic and clinical implications are described

Genomic and ecological evidence shed light on the recent demographic history of two related invasive insects

Hypogeococcus pungens is a species complex native to southern South America that is composed of at least five putative species, each one specialized in the use of different host plants. Two of these undescribed species were registered as invasive in Central and North America: Hyp-C is a cactophagous mealybug that became an important pest that threatens endemic cactus species in Puerto Rico, and Hyp-AP feeds on Amaranthaceae and Portulacaceae hosts, but does not produce severe damage to the host plants. We quantified genomic variation and investigated the demographic history of both invasive species by means of coalescent-based simulations using high throughput sequencing data. We also evaluated the incidence of host plant infestation produced by both species and used an ecological niche modeling approach to assess potential distribution under current and future climatic scenarios. Our genetic survey evinced the footprints of strong effective population size reduction and signals of genetic differentiation among populations within each species. Incidence of plant attacks varied between species and among populations within species, with some host plant species preferred over others. Ecological niche modeling suggested that under future climatic scenarios both species would expand their distribution ranges in Puerto Rico. These results provide valuable information for the design of efficient management and control strategies of the Puerto Rican cactus pest and shed light on the evolutionary pathways of biological invasions.Fil: Poveda Martínez, Daniel Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Salinas, Nicolas Alejandro. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aguirre, María Belén. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez Restrepo, Andrés Fernando. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hight, Stephen. United States Department of Agriculture; Estados UnidosFil: Díaz-Soltero, Hilda. United States Department of Agriculture; Estados UnidosFil: Logarzo, Guillermo Alejandro. Fundación para el Estudio de Especies Invasivas; ArgentinaFil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentin

Plan de Campaña Pedagógica y Sensibilización Vial en el Municipio de Túquerres

El proyecto “PLAN DE CAMPAÑA PEDAGÓGICA Y SENSIBILIZACIÓN VIAL EN EL MUNICIPIO DE TÚQUERRES” se llevará a cabo a través de campañas, capacitaciones, brigadas y encuentros que permitan desarrollar un adecuado proceso de sensibilización cultural que fomente el uso diario de la bicicleta. El proyecto se realizará en el departamento de Nariño, específicamente en el municipio de Túquerres, y será controlado y auspiciado por la alcaldía del municipio, el proyecto será transversal al actual plan de gobierno y buscará complementar el trabajo grupos de la alcaldía de mejoramiento de la calidad de vida de los habitantes del municipio. El proyecto es sin ánimo de lucro y tendrá un enfoque social.The project " PLAN DE CAMPAÑA PEDAGÓGICA Y SENSIBILIZACIÓN VIAL EN EL MUNICIPIO DE TÚQUERRES " will be carried out through campaigns, trainings, brigades, and meetings that allow the development of an adequate process of cultural awareness that encourages the daily use of bicycles. The project will be carried out in the department of Nariño, specifically in the municipality of Túquerres Nariño, and will be controlled and sponsored by the mayor's office of the municipality, the project will be transversal to the current government plan and will seek to complement the working groups of the mayor's office of improvement of the quality of life of the inhabitants of the municipality. The project is non-profit and will have a social approach

Acute Coronary Syndrome: Acute Myocardial Infarction of the Lower Face plus Catheterization. Case Report

El síndrome coronario agudo (SCA) es causado por un desequilibrio entre el consumo de oxígeno del miocardio y el flujo sanguíneo, que puede ser causado por una disminución grave del suministro de sangre o un aumento de la demanda que no puede compensarse con un aumento del flujo sanguíneo. La manifestación más grave del SCA es una disminución dramática del flujo sanguíneo causada por la formación de un coágulo sanguíneo oclusivo sobre una placa aterosclerótica que se rompe en una arteria coronaria epicárdica. El síndrome coronario agudo sin elevación del ST, en muchos pacientes los cambios en el ECG son mínimos o reflejan un estado de reperfusión (inversión de la porción terminal de las ondas T o cambios T inespecíficos sin desviación significativa del segmento ST). Sin embargo, durante la isquemia activa, se pueden detectar cambios en el ECG, incluida la desviación del segmento ST y cambios en la morfología de las ondas T y U. Se reporta paciente femenina de 42 años con antecedentes de importancia acude por reportar dolor epigástrico tipo quemante urente de intensidad 6/10 según la escala de EVA, que se irradia a mandíbula, brazo izquierdo.Acute coronary syndrome (ACS) is caused by an imbalance between myocardial oxygen consumption and blood flow, which can be caused by a severe decrease in blood supply or an increase in demand that cannot be compensated by an increase in blood flow. Blood flow. The most severe manifestation of ACS is a dramatic decrease in blood flow caused by the formation of an occlusive blood clot over an atherosclerotic plaque that ruptures into an epicardial coronary artery. In non-ST elevation acute coronary syndrome, in many patients the ECG changes are minimal or reflect a state of reperfusion (inversion of the terminal portion of the T waves or nonspecific T changes without significant ST segment deviation). However, during active ischemia, changes can be detected on the ECG, including ST segment deviation and changes in T and U wave morphology. A 42-year-old female patient with a significant history comes to report burning burning epigastric pain of intensity 6/10 according to the VAS scale, which radiates to the jaw and left arm

Lower IgG somatic hypermutation rates during acute dengue virus infection is compatible with a germinal center-independent B cell response

Reads, clonal groups, and lineages numbers per individual and condition. The table shows the number of raw and used sequences as well as the number of clonotypes and lineages per individual. (XLS 30 kb

Phenolic compounds and antioxidant activity of a functional honey-added marshmallow

Objective: To evaluate the effect of the addition of honey and the ºBloom of gelatin on the phenol content and antioxidant capacity of a marshmallow. Design/methodology/approach: A 32 factorial design was carried out adding honey concentrations of 0, 50 and 75 % and grenetins of 265, 300 and 315 ºBloom. Results: The addition of honey increased the phenol content and antioxidant activity by more than 45 % compared to samples without honey. Sensory analysis did not show differences in taste, smell and mouthfeel between samples with and without honey, however, higher ºBloom improved the texture and appearance of the marshmallows. Limitations of the study/implications: The sweetness of the marshmallows with honey increased with the concentration of the honey, being excessive for consumers. Findings/conclusions: Marshmallows made with honey are a viable alternative for introduction to the confectionery market.Objective: To evaluate the effect of adding honey and the gelatin degrees Bloom in the phenolic content and antioxidant capacity of a marshmallow product. Design/methodology/approach: A 32 factorial design was carried out adding 0, 50 and 75% honey concentrations and 265, 300 and 315 degrees Bloom of gelatin. Results: Adding of honey increased the phenolic content and antioxidant activity by 45% compared to samples without honey. Sensory analysis showed no differences in flavor, aroma and mouthfeel between samples with and without honey, however, higher degrees bloom improved the texture and appearance of the marshmallows. Limitations of the study/implications: The sweetness of honey-added marshmallows increased with the honey concentration, being excessive for consumers. Findings/conclusions: Functional honey-added marshmallow is a viable alternative that can be feasibly introduced to the confectionery market