Postprandial apoE isoform and conformational changes associated with VLDL lipolysis products modulate monocyte inflammation.

ObjectivePostprandial hyperlipemia, characterized by increased circulating very low-density lipoproteins (VLDL) and circulating lipopolysaccharide (LPS), has been proposed as a mechanism of vascular injury. Our goal was to examine the interactions between postprandial lipoproteins, LPS, and apoE3 and apoE4 on monocyte activation.Methods and resultsWe showed that apoE3 complexed to phospholipid vesicles attenuates LPS-induced THP-1 monocyte cytokine expression, while apoE4 increases expression. ELISA revealed that apoE3 binds to LPS with higher affinity than apoE4. Electron paramagnetic resonance (EPR) spectroscopy of site-directed spin labels placed on specific amino acids of apoE3 showed that LPS interferes with conformational changes normally associated with lipid binding. Specifically, compared to apoE4, apoE bearing the E3-like R112→Ser mutation displays increased self association when exposed to LPS, consistent with a stronger apoE3-LPS interaction. Additionally, lipolysis of fasting VLDL from normal human donors attenuated LPS-induced TNFα secretion from monocytes to a greater extent than postprandial VLDL, an effect partially reversed by blocking apoE. This effect was reproduced using fasting VLDL lipolysis products from e3/e3 donors, but not from e4/e4 subjects, suggesting that apoE3 on fasting VLDL prevents LPS-induced inflammation more readily than apoE4.ConclusionPostprandial apoE isoform and conformational changes associated with VLDL dramatically modulate vascular inflammation

Simultaneous Multi-Wavelength Observations of Magnetic Activity in Ultracool Dwarfs. II. Mixed Trends in VB10 and LSR1835+32 and the Possible Role of Rotation

[Abridged] As part of our on-going investigation of magnetic activity in ultracool dwarfs we present simultaneous radio, X-ray, UV, and optical observations of LSR1835+32 (M8.5), and simultaneous X-ray and UV observations of VB10 (M8), both with a duration of about 9 hours. LSR1835+32 exhibits persistent radio emission and H-alpha variability on timescales of ~0.5-2 hr. The detected UV flux is consistent with photospheric emission, and no X-ray emission is detected to a deep limit of L_X/L_bol<10^-5.7. The H-alpha and radio emission are temporally uncorrelated, and the ratio of radio to X-ray luminosity exceeds the correlation seen in F-M6 stars by >2x10^4. Similarly, L_Halpha/L_X>10 is at least 30 times larger than in early M dwarfs, and eliminates coronal emission as the source of chromospheric heating. The lack of radio variability during four rotations of LSR1835+32 requires a uniform stellar-scale field of ~10 G, and indicates that the H-alpha variability is dominated by much smaller scales, <10% of the chromospheric volume. VB10, on the other hand, shows correlated flaring and quiescent X-ray and UV emission, similar to the behavior of early M dwarfs. Delayed and densely-sampled optical spectra exhibit a similar range of variability amplitudes and timescales to those seen in the X-rays and UV, with L_Halpha/L_X~1. Along with our previous observations of the M8.5 dwarf TVLM513-46546 we conclude that late M dwarfs exhibit a mix of activity patterns, which points to a transition in the structure and heating of the outer atmosphere by large-scale magnetic fields. We find that rotation may play a role in generating the fields as evidenced by a tentative correlation between radio activity and rotation velocity. The X-ray emission, however, shows evidence for super-saturation at vsini>25 km/s.Comment: Submitted to Ap

XMM-Newton Finds That SAX J1750.8-2900 May Harbor the Hottest, Most Luminous Known Neutron Star

We have performed the first sensitive X-ray observation of the low-mass X-ray binary SAX J1750.8-2900 in quiescence with XMM-Newton. The spectrum was fit to both a classical black body model, and a non-magnetized, pure hydrogen neutron star atmosphere model. A power law component was added to these models, but we found that it was not required by the fits. The distance to SAX J1750.8-2900 is known to be D = 6.79 kpc from a previous analysis of photospheric radius expansion bursts. This distance implies a bolometric luminosity (as given by the NS atmosphere model) of (1.05 +/- 0.12) x 10^34 (D/6.79 kpc)^2 erg s^-1, which is the highest known luminosity for a NS LMXB in quiescence. One simple explanation for this surprising result could be that the crust and core of the NS were not in thermal equilibrium during the observation. We argue that this was likely not the case, and that the core temperature of the NS in SAX J1750.8-2900 is unusually high

Coronal X-Ray Emission from the Stellar Companions to Transiently Accreting Black Holes

Observations of soft X-ray transients (SXTs) in quiescence have found that the binaries harboring black holes are fainter than those that contain a neutron star. Narayan and collaborators postulated that the faint X-ray emission from black hole binaries was powered by an advection dominated accretion flow (ADAF). We explore an alternative explanation for the quiescent X-ray emission from the black hole systems: coronal emission from the rapidly rotating optical companion. This is commonly observed and well studied in other tidally locked binaries, such as the RS CVns. We show that two of the three X-ray detected black hole binaries (A0620-00 and GRO J1655-40) exhibit X-ray fluxes entirely consistent with coronal emission. The X-ray spectra of these objects should be best fit with thermal Raymond-Smith models rich in lines when coronal emission predominates. One black hole system (V404 Cyg) is too X-ray bright to be explained as coronal emission. The quiescent X-ray emission from the neutron star binaries is far too bright for coronal emission. It might be that all SXT's have variable accretion rates in quiescence and that the basal quiescent X-ray flux is set by either coronal emission from the companion or -- when present -- by thermal emission from the neutron star. We also show that the lithium abundances in the black hole systems are comparable to those in the RS CVns, reducing the need for production mechanisms that involve the compact object.Comment: ApJ, accepted (v541; Oct 1, 2000); Changes to figures and tables, minor modifications to text. Uses emulateapj.sty. 14 pages, 3 figure

Chandra X-ray Observations of 12 Millisecond Pulsars in the Globular Cluster M28

We present a Chandra X-ray Observatory investigation of the millisecond pulsars (MSPs) in the globular cluster M28 (NGC 6626). In what is one of the deepest X-ray observations of a globular cluster, we firmly detect seven and possibly detect two of the twelve known M28 pulsars. With the exception of PSRs B1821-24 and J1824-2452H, the detected pulsars have relatively soft spectra, with X-ray luminosities 10^30-31 ergs s^-1 (0.3-8 keV),similar to most "recycled" pulsars in 47 Tucanae and the field of the Galaxy, implying thermal emission from the pulsar magnetic polar caps. We present the most detailed X-ray spectrum to date of the energetic PSR B1821-24. It is well described by a purely non-thermal spectrum with spectral photon index 1.23 and luminosity 1.4x10^33Theta(D/5.5 kpc)^2 ergs s^-1 (0.3-8 keV), where Theta is the fraction of the sky covered by the X-ray emission beam(s). We find no evidence for the previously reported line emission feature around 3.3 keV, most likely as a consequence of improvements in instrument calibration. The X-ray spectrum and pulse profile of PSR B1821--24 suggest that the bulk of unpulsed emission from this pulsar is not of thermal origin, and is likely due to low-level non-thermal magnetospheric radiation, an unresolved pulsar wind nebula, and/or small-angle scattering of the pulsed X-rays by interstellar dust grains. The peculiar binary PSR J1824-2452H shows a relatively hard X-ray spectrum and possible variability at the binary period, indicative of an intrabinary shock formed by interaction between the relativistic pulsar wind and matter from its non-degenerate companion star.Comment: 9 pages, 6 figures. Accepted for publication in the Astophysical Journa

The X-ray spectrum of a disk illuminated by ions

The X-ray spectrum from a cool disk embedded in an ion supported torus is computed. The interaction of the hot ions with the disk increases the hard X-ray luminosity of the system}. A surface layer of the disk is heated by the protons from the torus. The Comptonized spectrum produced by this layer has a shape that depends only weakly on the incident energy flux and the distance from the accreting compact object. It consists of a `blue bump' of unComptonized soft photons and a flat high energy tail, reminiscent of the observed spectra. The hard tail becomes flatter as the thermalization depth in the cool disk is increased. Further evidence for ion illumination are the Li abundance in the secondaries of low mass X-ray binaries and the 450 keV lines sometimes seen in black-hole transient spectra.Comment: 7p, to appear in Monthly Notice

The Unusual X-ray Binaries of the Globular Cluster NGC 6652

Our 5 ks Chandra ACIS-S observation of the globular cluster NGC 6652 detected 7 X-ray sources, 3 of which are previously unidentified. This cluster hosts a well-known bright low-mass X-ray binary, source A (or XB 1832-330). Source B shows unusual rapid flaring variability, with an average L_X(0.5-10 keV) ~2*10^{34} ergs/s, but with minutes-long flares up to L_X=9*10^{34} ergs/s. Its spectrum can be fit by an absorbed power-law of photon index Gamma~1.24, and hardens as the countrate decreases. This suggests that part or all of the variation might be due to obscuration by the rim of a highly inclined accretion disk. Sources C and D, with L_X ~10^{33} ergs/s, have soft and unusual spectra. Source C requires a very soft component, with a spectrum peaking at 0.5 keV, which might be the hot polar cap of a magnetically accreting polar cataclysmic variable. Source D shows a soft spectrum (fit by a power-law of photon index ~2.3) with marginal evidence for an emission line around 1 keV; its nature is unclear. The faint new sources E, F, and G have luminosities of 1-2*10^{32} ergs/s, if associated with the cluster (which is likely). E and F have relatively hard spectra (consistent with power-laws with photon index ~1.5). G lacks soft photons, suggesting absorption with N_H>10^{22} cm^{-2}.Comment: ApJ, in press. 9 pages, 9 figures (3 in color

Discovery of a Second Transient Low-Mass X-ray Binary in the Globular Cluster NGC 6440

We have identified a new transient luminous low-mass X-ray binary, NGC 6440 X-2, with Chandra/ACIS, RXTE/PCA, and Swift/XRT observations of the globular cluster NGC 6440. The discovery outburst (July 28-31, 2009) peaked at L_X~1.5*10^36 ergs/s, and lasted for <4 days above L_X=10^35 ergs/s. Four other outbursts (May 29-June 4, Aug. 29-Sept. 1, Oct. 1-3, and Oct. 28-31 2009) have been observed with RXTE/PCA (identifying millisecond pulsations, Altamirano et al. 2009a) and Swift/XRT (confirming a positional association with NGC 6440 X-2), with similar peak luminosities and decay times. Optical and infrared imaging did not detect a clear counterpart, with best limits of V>21, B>22 in quiescence from archival HST imaging, g'>22 during the August outburst from Gemini-South GMOS imaging, and J>~18.5$ and K>~17 during the July outburst from CTIO 4-m ISPI imaging. Archival Chandra X-ray images of the core do not detect the quiescent counterpart, and place a bolometric luminosity limit of L_{NS}< 6*10^31 ergs/s (one of the lowest measured) for a hydrogen atmosphere neutron star. A short Chandra observation 10 days into quiescence found two photons at NGC 6440 X-2's position, suggesting enhanced quiescent emission at L_X~6*10^31 ergs/s . NGC 6440 X-2 currently shows the shortest recurrence time (~31 days) of any known X-ray transient, although regular outbursts were not visible in the bulge scans before early 2009. Fast, low-luminosity transients like NGC 6440 X-2 may be easily missed by current X-ray monitoring.Comment: 13 pages (emulateapj), 8 (color) figures, ApJ in press. Revised version adds 5th outburst (Oct./Nov. 2009), additional discussion of possible causes of short outburst recurrence time

Chandra X-Ray Observatory Observations of the Globular Cluster M71

We observed the nearby, low-density globular cluster M71 (NGC 6838) with the Chandra X-ray Observatory to study its faint X-ray populations. Five X-ray sources were found inside the cluster core radius, including the known eclipsing binary millisecond pulsar (MSP) PSR J1953+1846A. The X-ray light curve of the source coincident with this MSP shows marginal evidence for periodicity at the binary period of 4.2 h. Its hard X-ray spectrum and luminosity resemble those of other eclipsing binary MSPs in 47 Tuc, suggesting a similar shock origin of the X-ray emission. A further 24 X-ray sources were found within the half-mass radius, reaching to a limiting luminosity of 1.5 10^30 erg/s (0.3-8 keV). From a radial distribution analysis, we find that 18+/-6 of these 29 sources are associated with M71, somewhat more than predicted, and that 11+/-6 are background sources, both galactic and extragalactic. M71 appears to have more X-ray sources between L_X=10^30--10^31 erg/s than expected by extrapolating from other studied clusters using either mass or collision frequency. We explore the spectra and variability of these sources, and describe the results of ground-based optical counterpart searches.Comment: 36 pages including 7 figures and 8 tables, accepted by The Astrophysical Journa

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence baseline. This article shows that baseline estimation errors are directly reflected in the observed PCR efficiency values and are thus propagated exponentially in the estimated starting concentrations as well as ‘fold-difference’ results. Because of the unknown origin and kinetics of the baseline fluorescence, the fluorescence values monitored in the initial cycles of the PCR reaction cannot be used to estimate a useful baseline value. An algorithm that estimates the baseline by reconstructing the log-linear phase downward from the early plateau phase of the PCR reaction was developed and shown to lead to very reproducible PCR efficiency values. PCR efficiency values were determined per sample by fitting a regression line to a subset of data points in the log-linear phase. The variability, as well as the bias, in qPCR results was significantly reduced when the mean of these PCR efficiencies per amplicon was used in the calculation of an estimate of the starting concentration per sample