Comet C/2004 Q2 (MACHHOLZ): Parent Volatiles, a Search for Deuterated Methane, and Constraint on the CH4 Spin Temperature

High-dispersion (l/dl ~ 25,000) infrared spectra of Comet C/2004 Q2 (Machholz) were acquired on Nov. 28-29, 2004, and Jan. 19, 2005 (UT dates) with NIRSPEC at the Keck-2 telescope on Mauna Kea. We detected H2O, CH4, C2H2, C2H6, CO, H2CO, CH3OH, HCN, and NH3 and we conducted a sensitive search for CH3D. We report rotational temperatures, production rates, and mixing ratios (with respect to H2O) at heliocentric distances of 1.49 AU (Nov. 2004) and 1.21 AU (Jan. 2005). We highlight three principal results: (1) The mixing ratios of parent volatiles measured at 1.49 AU and 1.21 AU agree within confidence limits, consistent with homogeneous composition in the mean volatile release from the nucleus of C/2004 Q2. Notably, the relative abundance of C2H6/C2H2 is substantially higher than those measured in other comets, while the mixing ratios C2H6/H2O, CH3OH/H2O, and HCN/H2O are similar to those observed in comets, referred to as "organics-normal". (2) The spin temperature of CH4 is > 35-38 K, an estimate consistent with the more robust spin temperature found for H2O. (3) We obtained a 3s upper limit of CH3D/CH4 < 0.020 (D/H < 0.005). This limit suggests that methane released from the nucleus of C/2004 Q2 is not dominated by a component formed in extremely cold (near 10 K) environments. Formation pathways of both interstellar and nebular origin consistent with the measured D/H in methane are discussed. Evaluating the relative contributions of these pathways requires further modeling of chemistry including both gas-phase and gas-grain processes in the natal interstellar cloud and in the protoplanetary disk.Comment: Accepted by The Astrophysical Journa

Update of the audit plan: Health quality improvement (PAMEC) of the Pain Relief and Palliative Medicine Unit (UDOP SAS) IPS of 2017

Se pretende actualizar el Programa de Auditoria para el Mejoramiento de la Calidad de la Atención en Salud PAMEC de la Unidad de Alivio del Dolor y Medicina Paliativa UDOP SAS y motivar a la institución a tomar conciencia sobre la importancia de la creación de una cultura organizacional que se enfoque en la seguridad del paciente, mediante la aplicación de un mejoramiento continuo a sus servicios asistenciales especializados, incrementando el beneficio a los usuarios, minimizando, de esta manera, los riesgos que afectan la calidad de la atención, en concordancia con el Decreto 1011 del 03 de Abril de 2006, el cual en su artículo 32, regula como parámetro, la auditoría para el mejoramiento de la calidad de la atención de salud Para el análisis de la calidad de la atención y de la percepción de la satisfacción de los usuarios, se procede a revisar la información de la situación de la institución consignada en el PAMEC actual, con el fin de determinar claramente factores que 10 afecten la insatisfacción de las necesidades de atención del usuario, así mismo, la ejecución de los procesos y efectividad en la implementación del PAMEC en la institución, con el objeto de apoyar a la institución en la creación de planes de mejoramiento continuo. La metodología utilizada para la obtención de resultados es PHVA (Planear, Hacer, Verificar y Actuar) con la utilización de instrumentos guía y documentación fuente relacionada.FORMULACIÓN DEL PROBLEMA MARCO TEORICO OBJETIVOS METODOLOGIA RESULTADOS CONCLUSIONES RECOMENDACIONES BIBLIOGRAFÍA ANEXOSEspecializaciónIt is intended to update the Audit Program for the Improvement of the Quality of Health Care PAMEC of the Pain Relief and Palliative Medicine Unit UDOP SAS and motivate the institution to become aware of the importance of creating an organizational culture that focuses on patient safety, through the application of continuous improvement to its specialized care services, increasing the benefit to users, thus minimizing the risks that affect the quality of care, in accordance with Decree 1011 of April 3, 2006, which in its article 32, regulates as a parameter, the audit for the improvement of the quality of health care For the analysis of the quality of care and the perception of user satisfaction , we proceed to review the information of the situation of the institution consigned in the current PAMEC, in order to clearly determine factors that affect the dissatisfaction of the user's attention needs, likewise, the execution of the processes and effectiveness in the implementation of PAMEC in the institution, in order to support the institution in the creation of continuous improvement plans. The methodology used to obtain results is PDCA (Plan, Do, Verify and Act) with the use of guiding instruments and related source documentation.Modalidad Presencia

Dual-energy X-ray absorptiometry study of body composition in patients with lipodystrophy

Inst Estadual Diabet & Endocrinol, Metab Unit, Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Endocrinol & Metab, São Paulo, BrazilDENSSO, Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Dept Endocrinol & Metab, São Paulo, BrazilWeb of Scienc

Galectin-1 expression imprints a neurovascular phenotype in proliferative retinopathies and delineates responses to anti-VEGF

Neovascular retinopathies are leading causes of irreversible blindness. Although vascular endothelial growth factor (VEGF) inhibitors have been established as the mainstay of current treatment, clinical management of these diseases is still limited. As retinal impairment involves abnormal neovascularization and neuronal degeneration, we evaluated here the involvement of galectin-1 in vascular and non-vascular alterations associated with retinopathies, using the oxygen-induced retinopathy (OIR) model. Postnatal day 17 OIR mouse retinas showed the highest neovascular profile and exhibited neuro-glial injury as well as retinal functional loss, which persisted until P26 OIR. Concomitant to VEGF up-regulation, galectin-1 was highly expressed in P17 OIR retinas and it was mainly localized in neovascular tufts. In addition, OIR induced remodelling of cell surface glycophenotype leading to exposure of galectin-1-specific glycan epitopes. Whereas VEGF returned to baseline levels at P26, increased galectin-1 expression persisted until this time period. Remarkably, although anti-VEGF treatment in P17 OIR improved retinal vascularization, neither galectin-1 expression nor non-vascular and functional alterations were attenuated. However, this functional defect was partially prevented in galectin-1-deficient (Lgals1-/-) OIR mice, suggesting the importance of targeting both VEGF and galectin-1 as non-redundant independent pathways. Supporting the clinical relevance of these findings, we found increased levels of galectin-1 in aqueous humor from patients with proliferative diabetic retinopathy and neovascular glaucoma. Thus, using an OIR model and human samples, we identified a role for galectin-1 accompanying vascular and non-vascular retinal alterations in neovascular retinopathies.Fil: Ridano, Magali Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Subirada Caldarone, Paula Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Paz, Maria Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lorenc, Valeria Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Stupirski, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gramajo, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche | Comisión Nacional de Energía Atómica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología. Unidad Ejecutora Instituto de Nanociencia y Nanotecnología - Nodo Bariloche; ArgentinaFil: Luna, José Domingo. Clinica de Ojos Romagosa, Fundacion Ver; ArgentinaFil: Croci Russo, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Química. Cátedra de Química Biologica; ArgentinaFil: Garcia Sanchez, Maria Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentin

Docência no Ensino Superior em Tempos de Escola Sem Partido

This article aimed to present a systematic review about the scientific production regarding  the Non-partisan  schoolmovement  to  identify  the  most  frequent themes  of  this  production,  as  well  as  gender  issues  and  its  impact  in higher education. Data were collected during the month of April 2018 in Capes portal. A total of 30 articles  were  collected  and included in  the qualitative análises.  It was  evidencied  that  the  scientific  is  incipient  and  the  main  theme  is  the criticism to the moviment. Gender issue seems to be fragmented and not deeply discussed.Cet article visait à présenter une revue systématique de la production scientifique nationale sur le Mouvement École sans parti, visant à identifier les thèmes les plus fréquents de cette production, ainsi que les questions de genre qui sont entrelacées, en configurant une coupure spécifique pour discuter de l'impact du Mouvement École sans parti dans la formation des enseignants de l'enseignement supérieur. Les données ont été recueillies au cours du mois d'avril 2018 à partir du portail des périodiques de la coordination de l'amélioration du personnel de plus haut niveau, totalisant 30 études incluses dans l'analyse qualitative. Il a été prouvé que la production scientifique dans la région est naissante, et le thème de la plus grande pertinence est autour de la problématisation et de la critique du Mouvement École sans parti. La question du genre semble fragmentée plutôt que approfondie.Este artículo buscó presentar una revisión sistemática de la producción científica nacional sobre el Movimiento Escuela sin Partido, buscando identificar las temáticas más frecuentes de dicha producción, así como con las cuestiones de género en ella imbricadas, configurando un recorte específico para la discusión del tema el impacto del Movimiento Escuela sin Partido en la formación de los profesores en la Enseñanza Superior. Los datos fueron recolectados durante el mes de abril de 2018 junto al Portal de Periódicos de la Coordinación de Perfeccionamiento de Personal de Nivel Superior, totalizando 30 estudios incluidos en el análisis cualitativo. Se evidenció que la producción científica en el área es incipiente, y la temática de mayor relevancia se da en torno a la problematización y crítica del Movimiento Escuela sin Partido. La cuestión de género aparece de forma fragmentada y no profundizada.Este artigo buscou apresentar uma revisão sistemática da produção científica nacional sobre o Movimento Escola sem Partido, visando a identificar as temáticas mais frequentes da referida produção, bem como com as questões de gênero nela imbricadas, configurando um recorte específico para a discussão do impacto do Movimento Escola sem Partido na formação dos professores no Ensino Superior. Os dados foram coletados durante o mês de abril de 2018 junto ao Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, totalizando 31 estudos incluídos na análise qualitativa. Evidenciou-se que a produção científica na área é incipiente, e a temática de maior relevância se dá em torno da problematização e crítica do Movimento Escola sem Partido. A questão de gênero aparece de forma fragmentada e não aprofundada

Theranostic biomarkers and PARP-inhibitors effectiveness in patients with non-BRCA associated homologous recombination deficient tumors: Still looking through a dirty glass window?

: Breast cancer susceptibility gene 1 (BRCA1) and breast cancer susceptibility gene 2 (BRCA2) deleterious variants were the first and, still today, the main biomarkers of poly(ADP)ribose polymerase (PARP)-inhibitors (PARPis) benefit. The recent, increased, numbers of individuals referred for counseling and multigene panel testing, and the remarkable expansion of approved PARPis, not restricted to BRCA1/BRCA2-Pathogenic Variants (PVs), produced a strong clinical need for non-BRCA biomarkers. Significant limitations of the current testing and assays exist. The different approaches that identify the causes of Homologous Recombination Deficiency (HRD), such as the germline and somatic Homologous Recombination Repair (HRR) gene PVs, the testing showing its consequences, such as the genomic scars, or the novel functional assays such as the RAD51 foci testing, are not interchangeable, and should not be considered as substitutes for each other in clinical practice for guiding use of PARPi in non-BRCA, HRD-associated tumors. Today, the deeper knowledge on the significant relationship among all proteins involved in the HRR, not limited to BRCA, expands the possibility of a successful non-BRCA, HRD-PARPi synthetic lethality and, at the same time, reinforces the need for enhanced definition of HRD biomarkers predicting the magnitude of PARPi benefit

KIT/PDGFRA Variant Allele Frequency as Prognostic Factor in Gastrointestinal Stromal Tumors (GISTs): Results From a Multi-Institutional Cohort Study

Background: The patient selection for optimal adjuvant therapy in gastrointestinal stromal tumors (GISTs) is provided by nomogram based on tumor size, mitotic index, tumor location, and tumor rupture. Although mutational status is not currently used to risk assessment, tumor genotype showed a prognostic influence on natural history and tumor relapse. Innovative measures, such as KIT/PDGFRA-mutant-specific variant allele frequency (VAF) levels detection from next-generation sequencing (NGS), may act as a surrogate of tumor burden and correlate with prognosis and overall survival of patients with GIST, helping the choice for adjuvant treatment. Patients and methods: This was a multicenter, hospital-based, retrospective/prospective cohort study to investigate the prognostic role of KIT or PDGFRA-VAF of GIST in patients with radically resected localized disease. In the current manuscript, we present the results from the retrospective phase of the study. Results: Two-hundred (200) patients with GIST between 2015 and 2022 afferent to 6 Italian Oncologic Centers in the EURACAN Network were included in the study. The receiver operating characteristic (ROC) curves analysis was used to classify "low" vs. "high" VAF values, further normalized on neoplastic cellularity (nVAF). When RFS between the low and high nVAF groups were compared, patients with GIST with KIT/PDGFRA nVAF &gt; 50% showed less favorable RFS than patients in the group of nVAF ≤ 50% (2-year RFS, 72.6% vs. 93%, respectively; P = .003). The multivariable Cox regression model confirmed these results. In the homogeneous sub-population of intermediate-risk, patients with KIT-mutated GIST, the presence of nVAF &gt;50% was statistically associated with higher disease recurrence. Conclusion: In our study, we demonstrated that higher nVAF levels were independent predictors of GIST prognosis and survival in localized GIST patients with tumors harboring KIT or PDGFRA mutations. In the cohort of intermediate-risk patients, nVAF could be helpful to improve prognostication and the use of adjuvant imatinib

Postremission sequential monitoring of minimal residual disease by WT1 Q-PCR and multiparametric flow cytometry assessment predicts relapse and may help to address risk-adapted therapy in acute myeloid leukemia patients

Risk stratification in acute myeloid leukemia (AML) patients using prognostic parameters at diagnosis is effective, but may be significantly improved by the use of on treatment parameters which better define the actual sensitivity to therapy in the single patient. Minimal residual disease (MRD) monitoring has been demonstrated crucial for the identification of AML patients at high risk of relapse, but the best method and timing of MRD detection are still discussed. Thus, we retrospectively analyzed 104 newly diagnosed AML patients, consecutively treated and monitored by quantitative polymerase chain reactions (Q-PCR) on WT1 and by multiparametric flow cytometry (MFC) on leukemia-associated immunophenotypes (LAIPs) at baseline, after induction, after 1st consolidation and after 1st intensification. By multivariate analysis, the factors independently associated with adverse relapse-free survival (RFS) were: bone marrow (BM)-WT1 ≥ 121/10(4) ABL copies (P = 0.02) and LAIP ≥ 0.2% (P = 0.0001) (after 1st consolidation) (RFS at the median follow up of 12.5 months: 51% vs. 82% [P < 0.0001] and 57% vs. 81%, respectively [P = 0.0003]) and PB-WT1 ≥ 16/10(4) ABL copies (P = 0.0001) (after 1st intensification) (RFS 43% vs. 95% [P < 0.0001]) Our data confirm the benefits of sequential MRD monitoring with both Q-PCR and MFC. If confirmed by further prospective trials, they may significantly improve the possibility of a risk-adapted, postinduction therapy of AML