32 research outputs found
Enseñanza de la reproducción humana en secundaria a través del pensamiento sistémico en un entorno remoto
En el presente trabajo se expone la intervención educativa que he efectuado durante el prácticum, así como su relación con las asignaturas incluidas en el máster de Profesorado en Secundaria de la Universidad de Zaragoza. Comienza con una presentación personal y de mi currículum, junto a una introducción al trabajo y la contextualización de este en relación con el centro educativo donde realicé las prácticas. Sigue con un análisis didáctico de varias actividades realizadas durante el máster en relación con su aplicación en el prácticum. A continuación, se presenta una propuesta didáctica, que incluye un abordaje de pensamiento sistémico para trabajar el tema de la reproducción humana en 3º de ESO basado en la realización de una serie de actividades indicadas mediante la metodología de la clase invertida. Finalmente, se analiza la consecución de los objetivos de la propuesta y de las distintas actividades en el apartado de evaluación, y se proponen posibles mejoras a la propuesta, antes de terminar el trabajo con unas conclusiones finales.<br /
Leptin Receptor Gene Variant rs11804091 Is Associated with BMI and Insulin Resistance in Spanish Female Obese Children: A Case-Control Study
Leptin is an endocrine hormone that has a critical role in body weight homoeostasis and mediates its effects via the leptin receptor (LEPR). Common polymorphisms in the genes coding leptin receptors have been associated with metabolic abnormalities. We assessed the association of 28 LEPR polymorphisms with body mass index (BMI) and their relationship with obesity-related phenotypes, inflammation and cardiovascular disease risk biomarkers. A multicentre case-control study was conducted in 522 children (286 with obesity and 236 with normal-BMI). All anthropometric, metabolic factors and biomarkers were higher in children with obesity except apolipoprotein (Apo)-AI, cholesterol, high-density lipoprotein cholesterol (HDL-c), and adiponectin, which were lower in the obesity group; and glucose, low-density lipoprotein cholesterol (LDL-c), and matrix metalloproteinase-9 that did not differ between groups. We identified the associations between rs11208659, rs11804091, rs10157275, rs9436303 and rs1627238, and BMI in the whole population, as well as the association of rs11804091, rs10157275, and rs1327118 with BMI in the female group, although only the rs11804091 remained associated after Bonferroni correction (p = 0.038). This single nucleotide polymorphisms (SNP) was also associated with insulin (p = 0.004), homeostasis model assessment for insulin resistance (HOMA-IR) (p = 0.006), quantitative insulin sensitivity check index (QUICKI) (p = 0.005) and adiponectin (p = 0.046) after adjusting for age, Tanner stage and BMI. Our results show a sex-specific association between the rs11804091 and obesity suggesting an influence of this SNP on insulin resistance.This work was supported by Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I+D+I), Instituto de Salud Carlos III-Fondo de Investigación Sanitaria (PI020826, PI051968, PI1102042, and PI1600871), RETIC (Red SAMID RD12/0026/0015) and Fondo Europeo De Desarrollo Regional (FEDER)
Energy dense salty food consumption frequency is associated with diastolic hypertension in Spanish children
High blood pressure (BP) is a risk factor for cardiovascular disease and sodium consumption is related to high BP. Moreover, sugar-sweetened beverages (SSB) and the Dietary Approach to Stop Hypertension (DASH) influence BP. For this reason, we investigated whether: 1) children with risk of elevated BP had a higher consumption frequency (CF) of energy-dense salty foods (EDSF), high-sugary foods (HSF) and SSB or a low DASH score; and 2) children with a higher CF of EDSF showed a worse anthropometric and metabolic profile. Anthropometry, BP and general biochemical parameters were measured in 687 Spanish children (5-16 years) with normal or excess weight. A food frequency questionnaire was used to calculate EDSF, HSF and SSB consumption, and modified DASH score. Results showed that sex and pubertal stage influenced modified DASH score. Diastolic hypertension was associated to higher CF of EDSF in the whole sample and to higher CF of SSB in pubertal children, both independently of nutritional status. In addition, CF of EDSF was positively associated with CF of HSF and SSB and inversely associated with modified DASH score. Targeted policies and intervention programs, specific for different age ranges, should be established that aim to reduce salt consumption from snacks and processed foods, which could reduce HSF and SSB consumption as well
Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review
Over the last several years, the increasing prevalence of obesity has favored an intense study of adipose tissue biology and the precise mechanisms involved in adipocyte differentiation and adipogenesis. Adipocyte commitment and differentiation are complex processes, which can be investigated thanks to the development of diverse in vitro cell models and molecular biology techniques that allow for a better understanding of adipogenesis and adipocyte dysfunction associated with obesity. The aim of the present work was to update the different animal and human cell culture models available for studying the in vitro adipogenic differentiation process related to obesity and its co-morbidities. The main characteristics, new protocols, and applications of the cell models used to study the adipogenesis in the last five years have been extensively revised. Moreover, we depict co-cultures and three-dimensional cultures, given their utility to understand the connections between adipocytes and their surrounding cells in adipose tissue
Evaluation of the Predictive Ability, Environmental Regulation and Pharmacogenetics Utility of a BMI-Predisposing Genetic Risk Score during Childhood and Puberty
The authors would like to thank the Spanish children and parents who participated in
the study.Polygenetic risk scores (pGRSs) consisting of adult body mass index (BMI) genetic
variants have been widely associated with obesity in children populations. The implication of
such obesity pGRSs in the development of cardio-metabolic alterations during childhood as well
as their utility for the clinical prediction of pubertal obesity outcomes has been barely investigated
otherwise. In the present study, we evaluated the utility of an adult BMI predisposing pGRS for the
prediction and pharmacological management of obesity in Spanish children, further investigating
its implication in the appearance of cardio-metabolic alterations. For that purpose, we counted
on genetics data from three well-characterized children populations (composed of 574, 96 and 124
individuals), following both cross-sectional and longitudinal designs, expanding childhood and
puberty. As a result, we demonstrated that the pGRS is strongly associated with childhood BMI
Z-Score (B = 1.56, SE = 0.27 and p-value = 1.90 × 10−8
), and that could be used as a good predictor of
obesity longitudinal trajectories during puberty. On the other hand, we showed that the pGRS is not
associated with cardio-metabolic comorbidities in children and that certain environmental factors
interact with the genetic predisposition to the disease. Finally, according to the results derived from a
weight-reduction metformin intervention in children with obesity, we discarded the utility of the
pGRS as a pharmacogenetics marker of metformin response.Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I + D + I), Instituto de Salud Carlos III-Health Research Funding (FONDOS FEDER)
PI1102042
PI1102059
PI1601301
PI1600871Spanish Ministry of Health, Social and Equality, General Department for Pharmacy and Health Products
EC10-243
EC10-056
EC10-281
EC10-227Regional Government of Andalusia ("Plan Andaluz de investigacion, desarrollo e innovacion (2018)")
P18-RT-2248Mapfre Foundation ("Research grants by Ignacio H. de Larramendi 2017")Instituto de Salud Carlos III
IFI17/0004
Antioxidants and Oxidative Stress in Children: Influence of Puberty and Metabolically Unhealthy Status
Oxidative stress could help explain the relationship between childhood obesity and a
metabolically unhealthy (MU) status. Moreover, puberty could also influence this relationship,
since it entails physiological cardiometabolic changes. We aimed to evaluate plasma antioxidants
and oxidative stress biomarkers in MU and metabolically healthy (MH) prepubertal and pubertal
children and their associations with pro-inflammatory and endothelial damage biomarkers, taking
puberty into account. A total of 1444 Spanish children aged 3–17 years (48.9% males, 66% prepubertal,
47.1% with obesity) were recruited. Blood pressure, anthropometric and biochemical parameters
were measured, and children were categorized as having a MU or MH status according to risk factors.
Retinol, carotenes, tocopherols, total antioxidant capacity (TAC), oxidized low-density lipoprotein
and selected pro-inflammatory and endothelial damage biomarkers were analyzed. General linear
models adjusted for age, sex, recruitment center and body mass index, partial correlations and
stepwise linear regressions were performed. Lower carotenes and tocopherols levels were found in
MU than in MH children. Plasma TAC was lower in prepubertal and higher in pubertal children with
obesity compared to normal-weight children. Antioxidants and oxidative stress biomarkers showed
novel associations with several pro-inflammatory and endothelial damage biomarkers, with pubertal
differences, supporting the importance of considering both the antioxidant and oxidative stress status
and puberty in the prevention of metabolic diseases in childhood.Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I + D + I), Instituto de Salud Carlos III-Health Research Funding (FONDOS FEDER)
PI051968
PI11/01425
PI1102042
PI11/02059
PI16/01301
PI16/012
PI1600871CIBEROBN Network
CB12/03/30038
CB15/00131
CB15/0004
Cluster Analysis of Physical Activity Patterns, and Relationship with Sedentary Behavior and Healthy Lifestyles in Prepubertal Children: Genobox Cohort
Sedentary habits during childhood are associated with adverse health outcomes. The aim of this work was to cluster lifestyle behaviors and metabolic biomarkers to establish different patterns in children. Their physical and sedentary activities were evaluated by accelerometry, and questionnaires that included lifestyle behaviors, such as adherence to a Mediterranean diet, anthropometry and blood biochemical markers. Cluster analysis was performed to establish different groups based on physical activity levels. A total of 489 children were finally selected. Cluster 1 included children with a mostly sedentary state, whereas Cluster 3 included the most active children and Cluster 2 included children that did not fit into either the sedentary or the highly active groups. In Cluster 3, 56% of children were in a sports club, and a lower percentage used electronic devices in their rooms compared to the other groups. Cluster 1 children exhibited higher insulin, HOMA-IR and triacylglycerides with respect to the other groups. No differences were found regarding adherence to a Mediterranean diet. The choice to practice an extracurricular sport could be an influencing factor to increase exercise and ensure an active lifestyle in children. Reducing or limiting screen time mainly in children''s rooms could contribute to an active lifestyle
Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study
The World Health Organization has recommended performing at least 60 min a day of
moderate-to-vigorous physical activity (MVPA) and reducing sedentarism in children and adolescents
to offer significant health benefits and mitigate health risks. Physical fitness and sports practice
seem to improve oxidative stress (OS) status during childhood. However, to our knowledge, there
are no data regarding the influence of objectively-measured physical activity (PA) and sedentarism
on OS status in children and adolescents. The present study aimed to evaluate the influence of
moderate and vigorous PA and sedentarism on OS and plasma total antioxidant capacity (TAC) in a
selected Spanish population of 216 children and adolescents from the GENOBOX study. PA (light,
moderate, and vigorous) and sedentarism (i.e., sedentary time (ST)) were measured by accelerometry.
A Physical Activity-Sedentarism Score (PASS) was developed integrating moderate and vigorous
PA and ST levels. Urinary 8-hydroxy-20-deoxyguanosine (8-OHdG) and isoprostane F2 (F2-IsoPs),
as markers of OS, were determined by ELISA; and TAC was estimated by colorimetry using an
antioxidant kit. A higher PASS was associated with lower plasma TAC and urinary 8-OHdG and
F2-IsoPs, showing a better redox profile. Reduced OS markers (8-OHdG and F2-IsoPs) in children
with higher PASS may diminish the need of maintaining high concentrations of antioxidants in
plasma during rest to achieve redox homeostasis.Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica (I + D + I), Instituto de Salud Carlos III-Health Research Funding (FONDOS FEDER)
PI11/02042
PI11/02059
PI11/01425
PI16/00871
PI16/01301
PI16/01205RETIC (Redes temáticas de investigación cooperativa)
Red SAMID RD12/0026/0015Instituto de Salud Carlos III
European Commission
IFI17/00048Research Plan of the Vice-Rectorate of Research and Transfer of the University of Granada, Spai
Are Peripheral Biomarkers Determinants of Eating Styles in Childhood and Adolescence Obesity? A Cross-Sectional Study
Disturbances in eating behaviors have been widely related to obesity. However, little is known about the role of obesity-related biomarkers in shaping habitual patterns of eating be haviors (i.e., eating styles) in childhood. The objective of the present study was to explore the relationships between several biomarkers crucially involved in obesity (ghrelin, insulin resistance, and leptin/adiponectin ratio) and eating styles in children and adolescents with obesity. Seventy participants aged between 8 and 16 (56.2% men) fulfilled the Spanish version of the Dutch Eating Behavior Questionnaire for Children to measure external, emotional, and restrained eating styles. In addition, concentrations of ghrelin, leptin, adiponectin, insulin, and glucose were obtained through a blood test. Hierarchical multiple regression analyses controlling for age and sex were computed for each eating style. Results indicated that individuals with higher ghrelin concentration levels showed lower scores in restrained eating (β = −0.61, p < 0.001). The total model explained 32% of the variance of the restrained pattern. No other relationships between obesity-related biomarkers and eating behaviors were found. This study highlights that one of the obesity-risk factors, namely lower plasma ghrelin levels, is substantially involved in a well-known maladaptive eating style, restraint eating, in childhood obesity