596 research outputs found

    Just World Beliefs, Identity Development, and Social Justice Advocacy of Counselor Trainees

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    This was the first study exploring the relationship between the belief in a just world, identity development, and social justice advocacy (SJA). A mixed methods design was conducted using a nationwide sample of ninety-seven counselor-in-training participants. Hypotheses included positive correlation between identity development and SJA, negative correlation between belief in a just world and SJA, and negative correlation between belief in a just world and identity development. Results were not significant but provided implications for future research and counselor training programs

    E-participation in contemporary China: A comparison with conventional offline participation

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    Drawing on the resource theory of political participation, we compare the determinants of Internet-based e-participation and conventional offline political participation in China by employing data from an original survey conducted in 2013. We find that e-government and other online platforms provide more equal participation opportunities to Chinese citizens traditionally lacking political resources. Although non-party members and non-elites are disadvantaged in conventional offline participation, they are not in e-participation, especially through using e-government systems. Internet/computer access and Internet skills push individuals away from conventional offline participation, and frequent social media users are more likely to engage in e-participation. Taken together, these results suggest that e-government and other online platforms offer the genuine potential to expand the scope of participation and empowers those traditionally disadvantaged in China

    Determination of association constants between 5 '-guanosine monophosphate gel and aromatic compounds by capillary electrophoresis

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    Hydro gel formed by 5'-guanosine monophosphate (GMP) in the presence of a potassium ion is expected to exhibit interesting selectivity in capillary electrophoretic separations. Here, we estimated the conditional association constants between the hydro gel (G-gel) and aromatic compounds by capillary electrophoresis in order to investigate the separation selectivity that is induced by the G-gel. Several aromatic compounds were separated in a solution containing GMP and potassium ion at different concentrations. The association constants were calculated by correlating the electrophoretic mobilities of the analytes obtained experimentally using a concentration of G-gel. During semi-quantitative estimation, naphthalene derivatives had larger association constants (K-ass = 10.3-16.8) compared with those of benzene derivatives (K-ass = 3.91-5.31), which means that the binding sites of G-gel match better to a naphthalene ring than to a benzene ring. A hydrophobic interaction was also found when the association constants for alkyl resorcinol were compared with those of different hydrocarbon chains. The association constants of nucleobases and tryptophan ranged from 6.05 to 12.6, which approximated the intermediate values between benzene and naphthalene derivatives. Consequently, the selective interaction between G-gel and aromatic compounds was classified as one of three types: (1) an intercalation into stacked planar GMP tetramers; (2) a hydrophobic interaction with a long alkyl chain; or, (3) a small contribution of steric hindrance and/or hydrogen bonding with functional groups such as amino and hydroxyl groups

    Activated Carbon Produced from Agricultural Residues

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    A process for producing activated carbon from agricultural residues by heating the residues to a temperature in the range of about 250° C. to about 550° C. to volatilize organic compounds in the residues and to carbonize the residues and further heating to activate the carbonized residues. Activated carbon produced from agricultural residues

    Undated - George Wiggin, Jesse Ames, and others recommend Sergeant Joseph R. Conant for promotion

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    https://digitalmaine.com/cw_me_4th_regiment_corr/1009/thumbnail.jp

    Measurement of dissociation rate of biomolecular complexes using CE

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    Fluorescence anisotropy (FA), non-equilibrium CE of equilibrium mixtures (NECEEM) and high-speed CE were evaluated for measuring dissociation kinetics of peptide–protein binding systems. Fyn-SH3-SH2, a protein construct consisting of the src homology 2 (SH2) and 3 (SH3) domain of the protein Fyn, and a fluorescein-labeled phosphopeptide were used as a model system. All three methods gave comparable half-life of∼53 s for Fyn-SH3-SH2:peptide complex. Achieving satisfactory results by NECEEM required columns over 30 cm long. When using Fyn-SH2-SH3 tagged with glutathione S -transferase (GST) as the binding protein, both FA and NECEEM assays gave evidence of two complexes forming with the peptide, yet neither method allowed accurate measurement of dissociation rates for both complexes because of a lack of resolution. High-speed CE, with a 7 s separation time, enabled separation of both complexes and allowed determination of dissociation rate of both complexes independently. The two complexes had half-lives of 22.0±2.7 and 58.8±6.1 s, respectively. Concentration studies revealed that the GST-Fyn-SH3-SH2 protein formed a dimer so that complexes had binding ratios of 2:1 (protein-to-peptide ratio) and 2:2. Our results demonstrate that although all methods are suitable for 1:1 binding systems, high-speed CE is unique in allowing multiple complexes to be resolved simultaneously. This property allows determination of binding kinetics of complicated systems and makes the technique useful for discovering novel affinity interactions.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/61861/1/457_ftp.pd

    Histone deacetylases play distinct roles in telomeric VSG expression site silencing in African trypanosomes

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    African trypanosomes evade the host immune response through antigenic variation, which is achieved by periodically expressing different variant surface glycoproteins (VSGs). VSG expression is monoallelic such that only one of approximately 15 telomeric VSG expression sites (ESs) is transcribed at a time. Epigenetic regulation is involved in VSG control but our understanding of the mechanisms involved remains incomplete. Histone deacetylases are potential drug targets for diseases caused by protozoan parasites. Here, using recombinant expression we show that the essential Trypanosoma brucei deacetylases, DAC1 (class I) and DAC3 (class II) display histone deacetylase activity. Both DAC1 and DAC3 are nuclear proteins in the bloodstream stage parasite, while only DAC3 remains concentrated in the nucleus in insect-stage cells. Consistent with developmentally regulated localization, DAC1 antagonizes SIR2rp1-dependent telomeric silencing only in the bloodstream form, indicating a conserved role in the control of silent chromatin domains. In contrast, DAC3 is specifically required for silencing at VSG ES promoters in both bloodstream and insect-stage cells. We conclude that DAC1 and DAC3 play distinct roles in subtelomeric gene silencing and that DAC3 represents the first readily druggable target linked to VSG ES control in the African trypanosome

    Astonish Me! Recalling the Cabaret Spirit

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    Program for the 1999 RISD Wintersessoin Cabaret intiated by the Image and Word class in collaboration with students of RISD Cabaret 1998 and held at AS220. The presentation was conceived and performed in homage and celebration of past RISD Cabaret shows 1987-1998. Graphic Design: Ron Winter, Ji-Ho Sohn and Eric Urban.https://digitalcommons.risd.edu/liberalarts_cabaret_programs/1010/thumbnail.jp
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