Neonatal rhesus monkey is a potential animal model for studying pathogenesis of EV71 infection

AbstractData from limited autopsies of human patients demonstrate that pathological changes in EV71-infected fatal cases are principally characterized by clear inflammatory lesions in different parts of the CNS; nearly identical changes were found in murine, cynomolgus and rhesus monkey studies which provide evidence of using animal models to investigate the mechanisms of EV71 pathogenesis. Our work uses neonatal rhesus monkeys to investigate a possible model of EV71 pathogenesis and concludes that this model could be applied to provide objective indicators which include clinical manifestations, virus dynamic distribution and pathological changes for observation and evaluation in interpreting the complete process of EV71 infection. This induced systemic infection and other collected indicators in neonatal monkeys could be repeated; the transmission appears to involve infecting new monkeys by contact with feces of infected animals. All data presented suggest that the neonatal rhesus monkey model could shed light on EV71 infection process and pathogenesis

Characterizing Rockbursts and Analysis on Hilbert-Huang Transform Spectrum of Microseismic Events, Shuangjiangkou Hydropower Station, Based on Microseismic Monitoring

The Shuangjiangkou hydropower station in China has complex geological conditions with high in situ stress. During the tunnel excavation, rockbursts occurred frequently, which seriously affected construction progress. Microseismic (MS) monitoring technology was used to explore rock MS activities to predict rockbursts. The MS monitoring system can capture a large number of MS signals. Based on Hilbert–Huang transform (HHT) instantaneous frequency analysis technology, using MATLAB software (R2022a) to write a program to convert the MS waveform, the frequency and energy characteristics of MS signals at a certain time can be obtained. By analyzing the frequency and energy characteristics of every event, the microseism active areas can be determined, and then rockbursts can be predicted scientifically. This paper selected two different construction sites, which were the main powerhouse and the access tunnel in the main powerhouse, as the research background. Introducing HHT instantaneous time–frequency analysis technology conducted MS event dynamic analysis and predicted rockbursts. The HHT spectrum scientifically and comprehensively displayed MS signal frequency characteristics at a certain time and reflected the change laws of signal instantaneous energy and local abrupt change information. The results indicated that some parameter anomalies in the event spectrum can predict rockbursts. For complex tunnel construction conditions, the HHT time–frequency analysis technology can realize a new idea of using a single-channel signal to predict rockbursts, which was very meaningful

Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: A report of further observations

Background: To investigate the long-term effects on immunity of an inactivated enterovirus 71 (EV71) vaccine and its protective efficacy. Methods: A sub-cohort of 1,100 volunteers from Guangxi Province in China was eligible for enrolment and randomly administered either the EV71 vaccine or a placebo on days 0 and 28 in a phase III clinical trial and then observed for the following 2 years with approval by an independent ethics committee of Guangxi Zhuang Autonomous Region, China. Serum samples from the 350 participants who provided a full series of blood samples (at all the sampling points) within the 2-year period were collected. Vaccine-induced immune effects, including the neutralizing antibody titres and cross-protection against different genotypes of EV71, were examined. This study also evaluated the protective efficacy of this vaccine based upon clinical diagnosis. Results: This sub-cohort showed a >60 % drop-out rate over 2 years. The seroconversion rates among the 161 immunized subjects remained >95 % at the end of study. The geometric mean titres of neutralizing antibodies (anti-genotype C4) 360 days after vaccination in 350 subjects were 81.0 (subjects aged 6-11 months), 98.4 (12-23 months), 95.0 (24-35 months), and 81.8 (36-71 months). These titres subsequently increased to 423.1, 659.0, 545.0, and 321.9, respectively, at 540 days post-immunization (d.p.i.), and similar levels were maintained at 720 d.p.i. Higher IFN-γ/IL-4-specific responses to the C4 genotype of EV71 and cross-neutralization reactivity against major EV71 genotype strains were observed in the vaccine group compared to those in the placebo group. Five EV71-infected subjects were observed in the placebo-treated control group and none in the vaccine-immunized group in per-protocol analysis. Conclusion: These results are consistent with the induction of dynamic immune responses and protective efficacy of the vaccine against most circulating EV71 strains. Trial registration number: Clinicaltrials.gov, NCT01569581 , Trial registration date: March 2012 © 2015 Liu et al

Additional file 1: of Immunity and clinical efficacy of an inactivated enterovirus 71 vaccine in healthy Chinese children: a report of further observations

Detailed information for Method section (including the description of virus strains, summary of long-term phase III clinical trail, the ethics and protocol of long–term observation of phase III clinical trial, the consent form and additional supplemental data. Figure S1 legendA total of 1,100 participants, receiving either the vaccine or placebo, were observed during the surveillance period. The cumulative curves for the cases of HFMD induced by enterovirus 71 (EV71), coxsackievirus A16 (CA16), and other enteroviruses were estimated as a percentage among these participants with Kaplan–Meier survival curves during the period from the receipt of the first dose until 24 months thereafter. The inset shows the same data on an enlarged y-axis. Differences in the distributions of cases of HFMD among the individuals who received the placebo and those who received the vaccine were evaluated with a log-rank test. (A) Cumulative curve for the cases of HFMD induced by EV71. (B) Cumulative curve for the cases of HFMD induced by CA16. (C) Cumulative curve for the cases of HFMD induced by other enteroviruses. (PDF 2302 kb

CEPC Technical Design Report -- Accelerator

International audienceThe Circular Electron Positron Collider (CEPC) is a large scientific project initiated and hosted by China, fostered through extensive collaboration with international partners. The complex comprises four accelerators: a 30 GeV Linac, a 1.1 GeV Damping Ring, a Booster capable of achieving energies up to 180 GeV, and a Collider operating at varying energy modes (Z, W, H, and ttbar). The Linac and Damping Ring are situated on the surface, while the Booster and Collider are housed in a 100 km circumference underground tunnel, strategically accommodating future expansion with provisions for a Super Proton Proton Collider (SPPC). The CEPC primarily serves as a Higgs factory. In its baseline design with synchrotron radiation (SR) power of 30 MW per beam, it can achieve a luminosity of 5e34 /cm^2/s^1, resulting in an integrated luminosity of 13 /ab for two interaction points over a decade, producing 2.6 million Higgs bosons. Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons, facilitating precise measurements of Higgs coupling at sub-percent levels, exceeding the precision expected from the HL-LHC by an order of magnitude. This Technical Design Report (TDR) follows the Preliminary Conceptual Design Report (Pre-CDR, 2015) and the Conceptual Design Report (CDR, 2018), comprehensively detailing the machine's layout and performance, physical design and analysis, technical systems design, R&D and prototyping efforts, and associated civil engineering aspects. Additionally, it includes a cost estimate and a preliminary construction timeline, establishing a framework for forthcoming engineering design phase and site selection procedures. Construction is anticipated to begin around 2027-2028, pending government approval, with an estimated duration of 8 years. The commencement of experiments could potentially initiate in the mid-2030s