Porque detrás de cada historia vive un mensaje especial

Mensaje Especial es un ciclo de 8 programas de televisión de 24 minutos, donde el testimonio de la realidad de 10 mujeres contemporáneas -de diferentes edades y orígenes- es el protagonista. Es una tesis de producción, la cual no pretende dar un aporte de tipo teórico, por tanto no se hace énfasis en demostrar la investigación previa sino su realización. El ciclo plantea una problemática hacia el espectador en cuanto a su cotidianeidad, intentando generar un espacio televisivo de identificación de género, desde el punto de vista de las mujeres. Cada programa cuenta con un nombre y un homenaje a una mujer diferente. A lo largo de 3 bloques: se presenta el ciclo, el programa (tema), se rinde homenaje a una mujer reconocida públicamente, se desarrolla el tema a través de los testimonios, los cuales dan una enseñanza de vida, se expone una campaña a favor de los derechos humanos y, finalmente, se presenta al próximo programa. El programa piloto trata sobre las relaciones amorosas hombre-mujer en la actualidad, el eje es el amor de pareja, su contrapartida la soledad y su antagonista la violencia. Se trata de revalorizar la vivencia individual para beneficio de otros, en un Mensaje Especial.Facultad de Periodismo y Comunicación Socia

Cellular Interaction of Human Eukaryotic Elongation Factor 1A Isoforms

Besides its canonical role in protein synthesis, the eukaryotic translation elongation factor 1A (eEF1A) is also involved in many other cellular processes such as cell survival and apoptosis. We showed that eEF1A phosphorylation by C-Raf in vitro occurred only in the presence of eEF1A1 and eEF1A2, thus suggesting that both isoforms interacted in cancer cells (heterodimer formation). This hypothesis was recently investigated in COS-7 cells where fluorescent recombinant eEF1A isoforms colocalized at the level of cytoplasm with a FRET signal more intense at plasma membrane level. Here, we addressed our attention in highlighting and confirming this interaction in a different cell line, HEK 293, normally expressing eEF1A1 but lacking the eEF1A2 isoform. To this end, His-tagged eEF1A2 was expressed in HEK 293 cells and found to colocalize with endogenous eEF1A1 in the cytoplasm, also at the level of cellular membranes. Moreover, FRET analysis showed, in this case, the appearance of a stronger signal mainly at the level of the plasma membrane. These results confirmed what was previously observed in COS-7 cells and strongly reinforced the interaction among eEF1A isoforms. Moreover, the formation of eEF1A heterodimer in cancer cells could also be important for cytoskeleton rearrangements rather than for phosphorylation, most likely occurring during cell survival and apoptosis

FSCN1 as a new druggable target in adrenocortical carcinoma

Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with a high risk of relapse and metastatic spread. The actin-bundling protein fascin (FSCN1) is overexpressed in aggressive ACC and represents a reliable prognostic indicator. FSCN1 has been shown to synergize with VAV2, a guanine nucleotide exchange factor for the Rho/Rac GTPase family, to enhance the invasion properties of ACC cancer cells. Based on those results, we investigated the effects of FSCN1 inactivation by CRISPR/Cas9 or pharmacological blockade on the invasive properties of ACC cells, both in vitro and in an in vivo metastatic ACC zebrafish model. Here, we showed that FSCN1 is a transcriptional target for β-catenin in H295R ACC cells and that its inactivation resulted in defects in cell attachment and proliferation. FSCN1 knock-out modulated the expression of genes involved in cytoskeleton dynamics and cell adhesion. When Steroidogenic Factor-1 (SF-1) dosage was upregulated in H295R cells, activating their invasive capacities, FSCN1 knock-out reduced the number of filopodia, lamellipodia/ruffles and focal adhesions, while decreasing cell invasion in Matrigel. Similar effects were produced by the FSCN1 inhibitor G2-044, which also diminished the invasion of other ACC cell lines expressing lower levels of FSCN1 than H295R. In the zebrafish model, metastases formation was significantly reduced in FSCN1 knock-out cells and G2-044 significantly reduced the number of metastases formed by ACC cells. Our results indicate that FSCN1 is a new druggable target for ACC and provide the rationale for future clinical trials with FSCN1 inhibitors in patients with ACC

A quantitative method for the study of HIV-1 and Mycobacterium tuberculosis co-infection.

M. tuberculosis and HIV-1 syndemic interactions are a major global health concern. Despite the clinical significance of co-infection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of co-infection is limited. Here, we use single-round infectious HIV-1 pseudo-typed viral-particles expressing GFP alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies

A Quantitative Method for the Study of HIV-1 and Mycobacterium tuberculosis Coinfection

Mycobacterium tuberculosis and human immunodeficiency virus-1 (HIV-1) syndemic interactions are a major global health concern. Despite the clinical significance of coinfection, our understanding of the cellular pathophysiology and the therapeutic pharmacodynamic impact of coinfection is limited. Here, we use single-round infectious HIV-1 pseudotyped viral particles expressing green fluorescent protein alongside M. tuberculosis expressing mCherry to study pathogenesis and treatment. We report that HIV-1 infection inhibited intracellular replication of M. tuberculosis and demonstrate the therapeutic activity of antiviral treatment (efavirenz) and antimicrobial treatment (rifampicin). The described method could be applied for detailed mechanistic studies to inform the development of novel treatment strategies

Vitamin D pathway gene polymorphisms, diet, and risk of postmenopausal breast cancer: a nested case-control study

INTRODUCTION: Vitamin D receptor (VDR) polymorphisms have been inconsistently associated with breast cancer risk. Whether risk is influenced by polymorphisms in other vitamin D metabolism genes and whether calcium or vitamin D intake modifies risk by genotype have not been evaluated. METHODS: We conducted a nested case-control study within the Cancer Prevention Study II Nutrition Cohort of associations between breast cancer and four VDR single-nucleotide polymorphisms (SNPs), Bsm1,Apa1,Taq1, and Fok1, a poly(A) microsatellite, and associated haplotypes (baTL and BAtS). We also examined one SNP in the 24-hydroxylase gene (CYP24A1) and two in the vitamin D-binding protein (group-specific component [GC]) gene. Participants completed a questionnaire on diet and medical history at baseline in 1992. This study includes 500 postmenopausal breast cancer cases and 500 controls matched by age, race/ethnicity, and date of blood collection. RESULTS: Incident breast cancer was not associated with any genotype examined. However, women with the Bsm1 bb SNP who consumed greater than the median intake of total calcium (≥902 mg/day) had lower odds of breast cancer compared to women with the Bb or BB genotype and less than the median calcium intake (odds ratio 0.61, 95% confidence interval 0.38 to 0.96; p(interaction )= 0.01). Similar interactions were observed for Taq1 (T allele) and the poly(A) (LL) repeat. CONCLUSION: We found no overall association between selected vitamin D pathway genes and postmenopausal breast cancer risk. However, certain VDR gene polymorphisms were associated with lower risk in women consuming high levels of calcium, suggesting that dietary factors may modify associations by VDR genotype

Mujeres y cultura en la Argentina del siglo XIX

Este libro es el resultado de buscar y rebuscar, acuciadas en un primer momento por una natural curiosidad hacia el siglo XIX, en el baúl de nuestras abuelas o incluso bisabuelas. Y nos encontramos no sólo con el pasado sino con nuestro pasado, rico, complejo, sorprendente en su infinita variedad de hallazgos. Experimentamos un sentimiento de ternura por esas mujeres y de profunda solidaridad ante el esfuerzo que realizaron, muchas veces en condiciones inhóspitas, incomprendidas por sus contemporáneos, varones y mujeres. No sólo mujeres con nombre propio sino mujeres generalmente anónimas forman una parte esencial de la historia no contada, oculta(da) en el polvo acumulado durante el transcurso de los años. Nuestra tarea, nuestra pasión es desenvolverla, descubrirla, deconstruirla. Un acto compartido entre muchas mujeres y algunos varones. En mayor o menor grado, cada artículo en Mujeres y cultura en la Argentina del siglo XIXcontribuye a este fin y también al de la construcción –la re-construcción- de la historia de mujeres en la Argentina. Hay todavía mucho por revolver en esos arcones. Sabemos que hay nombres escondidos todavía en publicaciones que debemos encontrar. Nombres de mujeres, sus labores, que han sido muy poco investigadas o que sencillamente desconocemos. Se trata de hacer visible una producción ignorada y una tradición desdibujada