283 research outputs found
Spin-polarized transport through a single-level quantum dot in the Kondo regime
Nonequilibrium electronic transport through a quantum dot coupled to
ferromagnetic leads (electrodes) is studied theoretically by the nonequilibrium
Green function technique. The system is described by the Anderson model with
arbitrary correlation parameter . Exchange interaction between the dot and
ferromagnetic electrodes is taken into account {\it via} an effective molecular
field. The following situations are analyzed numerically: (i) the dot is
symmetrically coupled to two ferromagnetic leads, (ii) one of the two
ferromagnetic leads is half-metallic with almost total spin polarization of
electron states at the Fermi level, and (iii) one of the two electrodes is
nonmagnetic whereas the other one is ferromagnetic. Generally, the Kondo peak
in the density of states (DOS) becomes spin-split when the total exchange field
acting on the dot is nonzero. The spin-splitting of the Kondo peak in DOS leads
to splitting and suppression of the corresponding zero bias anomaly in the
differential conductance.Comment: 9 pages, 7 figure
Reversible adsorption on a random site surface
We examine the reversible adsorption of hard spheres on a random site surface
in which the adsorption sites are uniformly and randomly distributed on a
plane. Each site can be occupied by one solute provided that the nearest
occupied site is at least one diameter away. We use a numerical method to
obtain the adsorption isotherm, i.e. the number of adsorbed particles as a
function of the bulk activity. The maximum coverage is obtained in the limit of
infinite activity and is known exactly in the limits of low and high site
density. An approximate theory for the adsorption isotherms, valid at low site
density, is developed by using a cluster expansion of the grand canonical
partition function. This requires as input the number of clusters of adsorption
site of a given size. The theory is accurate for the entire range of activity
as long as the site density is less than about 0.3 sites per particle area. We
also discuss a connection between this model and the vertex cover problem.Comment: 16 pages, 10 figure
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Toxicological Responses of α-Pinene-Derived Secondary Organic Aerosol and Its Molecular Tracers in Human Lung Cell Lines
Secondary organic aerosol (SOA) is a major component of airborne fine particulate matter (PM2.5) that contributes to adverse human health effects upon inhalation. Atmospheric ozonolysis of α-pinene, an abundantly emitted monoterpene from terrestrial vegetation, leads to significant global SOA formation; however, its impact on pulmonary pathophysiology remains uncertain. In this study, we quantified an increasing concentration response of three well-established α-pinene SOA tracers (pinic, pinonic, and 3-methyl-1,2,3-butanetricarboxylic acids) and a full mixture of α-pinene SOA in A549 (alveolar epithelial carcinoma) and BEAS-2B (bronchial epithelial normal) lung cell lines. The three aforementioned tracers contributed ∼57% of the α-pinene SOA mass under our experimental conditions. Cellular proliferation, cell viability, and oxidative stress were assessed as toxicological end points. The three α-pinene SOA molecular tracers had insignificant responses in both cell types when compared with the α-pinene SOA (up to 200 μg mL-1). BEAS-2B cells exposed to 200 μg mL-1 of α-pinene SOA decreased cellular proliferation to ∼70% and 44% at 24- and 48-h post exposure, respectively; no changes in A549 cells were observed. The inhibitory concentration-50 (IC50) in BEAS-2B cells was found to be 912 and 230 μg mL-1 at 24 and 48 h, respectively. An approximate 4-fold increase in cellular oxidative stress was observed in BEAS-2B cells when compared with untreated cells, suggesting that reactive oxygen species (ROS) buildup resulted in the downstream cytotoxicity following 24 h of exposure to α-pinene SOA. Organic hydroperoxides that were identified in the α-pinene SOA samples likely contributed to the ROS and cytotoxicity. This study identifies the potential components of α-pinene SOA that likely modulate the oxidative stress response within lung cells and highlights the need to carry out chronic exposure studies on α-pinene SOA to elucidate its long-term inhalation exposure effects. © 2021 American Chemical Society
Falsification Of The Atmospheric CO2 Greenhouse Effects Within The Frame Of Physics
The atmospheric greenhouse effect, an idea that many authors trace back to
the traditional works of Fourier (1824), Tyndall (1861), and Arrhenius (1896),
and which is still supported in global climatology, essentially describes a
fictitious mechanism, in which a planetary atmosphere acts as a heat pump
driven by an environment that is radiatively interacting with but radiatively
equilibrated to the atmospheric system. According to the second law of
thermodynamics such a planetary machine can never exist. Nevertheless, in
almost all texts of global climatology and in a widespread secondary literature
it is taken for granted that such mechanism is real and stands on a firm
scientific foundation. In this paper the popular conjecture is analyzed and the
underlying physical principles are clarified. By showing that (a) there are no
common physical laws between the warming phenomenon in glass houses and the
fictitious atmospheric greenhouse effects, (b) there are no calculations to
determine an average surface temperature of a planet, (c) the frequently
mentioned difference of 33 degrees Celsius is a meaningless number calculated
wrongly, (d) the formulas of cavity radiation are used inappropriately, (e) the
assumption of a radiative balance is unphysical, (f) thermal conductivity and
friction must not be set to zero, the atmospheric greenhouse conjecture is
falsified.Comment: 115 pages, 32 figures, 13 tables (some typos corrected
Synchronous diversification of Sulawesi's iconic artiodactyls driven by recent geological events
The high degree of endemism on Sulawesi has previously been suggested to have vicariant origins, dating back to 40 Ma. Recent studies, however, suggest that much of Sulawesi's fauna assembled over the last 15 Myr. Here, we test the hypothesis that more recent uplift of previously submerged portions of land on Sulawesi promoted diversification and that much of its faunal assemblage is much younger than the island itself. To do so, we combined palaeogeographical reconstructions with genetic and morphometric datasets derived from Sulawesi's three largest mammals: the babirusa, anoa and Sulawesi warty pig. Our results indicate that although these species most likely colonized the area that is now Sulawesi at different times (14 Ma to 2-3 Ma), they experienced an almost synchronous expansion from the central part of the island. Geological reconstructions indicate that this area was above sea level for most of the last 4 Myr, unlike most parts of the island. We conclude that emergence of land on Sulawesi (approx. 1-2 Myr) may have allowed species to expand synchronously. Altogether, our results indicate that the establishment of the highly endemic faunal assemblage on Sulawesi was driven by geological events over the last few million years
Physicochemical and biological characterization of chitosan-microRNA nanocomplexes for gene delivery to MCF-7 breast cancer cells
Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral vectors based on cationic natural polymers can form electrostatic complexes with negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated the physicochemical/biophysical properties of chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes and the biological responses of MCF-7 breast cancer cells cultured in vitro. Self-assembled CS–miRNA nanocomplexes were produced with a range of (+/−) charge ratios (from 0.6 to 8) using chitosans with various degrees of acetylation and molecular weight. The Z-average particle diameter of the complexes was <200 nm. The surface charge increased with increasing amount of chitosan. We observed that chitosan induces the base-stacking of miRNA in a concentration dependent manner. Surface plasmon resonance spectroscopy shows that complexes formed by low degree of acetylation chitosans are highly stable, regardless of the molecular weight. We found no evidence that these complexes were cytotoxic towards MCF-7 cells. Furthermore, CS–miRNA nanocomplexes with degree of acetylation 12% and 29% were biologically active, showing successful downregulation of target mRNA expression in MCF-7 cells. Our data, therefore, shows that CS–miRNA complexes offer a promising non-viral platform for breast cancer gene therapy
Gene Expression and Functional Studies of the Optic Nerve Head Astrocyte Transcriptome from Normal African Americans and Caucasian Americans Donors
To determine whether optic nerve head (ONH) astrocytes, a key cellular component of glaucomatous neuropathy, exhibit differential gene expression in primary cultures of astrocytes from normal African American (AA) donors compared to astrocytes from normal Caucasian American (CA) donors.We used oligonucleotide Affymetrix microarray (HG U133A & HG U133A 2.0 chips) to compare gene expression levels in cultured ONH astrocytes from twelve CA and twelve AA normal age matched donor eyes. Chips were normalized with Robust Microarray Analysis (RMA) in R using Bioconductor. Significant differential gene expression levels were detected using mixed effects modeling and Statistical Analysis of Microarray (SAM). Functional analysis and Gene Ontology were used to classify differentially expressed genes. Differential gene expression was validated by quantitative real time RT-PCR. Protein levels were detected by Western blots and ELISA. Cell adhesion and migration assays tested physiological responses. Glutathione (GSH) assay detected levels of intracellular GSH.Multiple analyses selected 87 genes differentially expressed between normal AA and CA (P<0.01). The most relevant genes expressed in AA were categorized by function, including: signal transduction, response to stress, ECM genes, migration and cell adhesion.These data show that normal astrocytes from AA and CA normal donors display distinct expression profiles that impact astrocyte functions in the ONH. Our data suggests that differences in gene expression in ONH astrocytes may be specific to the development and/or progression of glaucoma in AA
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