Analysis of coloured Grooved Ware sherds from the Ness of Brodgar, Orkney

To the accumulation of evidence of painted decoration applied to Neolithic and Early Bronze Age pottery in Britain and elsewhere in Europe we report here the presence of decoration in red, black and white on some third millennium BC Grooved ware pottery at the Ness of Brodgar on Orkney. As expected, the red was identified as an iron-rich material and black was carbon black. The white was prepared from calcined (cow) bone; however, its identification encountered some issues arising principally from the effects of the prevailing burial conditions on the bone. Furthermore, whereas the chemical and FTIR data were consistent with the presence of apatite, XRD indicated that the white had a significant content of silicate minerals. This finding has suggested that the white required a preparatory step which might have included the calcined bone bring ground to powder in a stone mortar before application to the vessel surface. The results obtained at Ness of Brodgar are reviewed in the light of archaeometric data on similarly decorated prehistoric pottery reported from sites in Europe

The C. elegans tailless/Tlx homolog nhr-67 regulates a stage-specific program of linker cell migration in male gonadogenesis

Cell migration is a common event during organogenesis, yet little is known about how migration is temporally coordinated with organ development. We are investigating stage-specific programs of cell migration using the linker cell (LC), a migratory cell crucial for male gonadogenesis of C. elegans. During the L3 and L4 larval stages of wild-type males, the LC undergoes changes in its position along the migratory route, in transcriptional regulation of the unc-5 netrin receptor and zmp-1 zinc matrix metalloprotease, and in cell morphology. We have identified the tailless homolog nhr-67 as a cell-autonomous, stage-specific regulator of timing in LC migration programs. In nhr-67-deficient animals, each of the L3 and L4 stage changes is either severely delayed or never occurs, yet LC development before the early L3 stage or after the mid-L4 stage occurs with normal timing. We propose that there is a basal migration program utilized throughout LC migration that is modified by stage-specific regulators such as nhr-67

To Cut a Long Story Short: Formal Chronological Modelling for the Late Neolithic Site of Ness of Brodgar, Orkney

YesIn the context of unanswered questions about the nature and development of the Late Neolithic in Orkney, we present a summary of research up to 2015 on the major site at the Ness of Brodgar, Mainland Orkney, concentrating on the impressive buildings. Finding sufficient samples for radiocarbon dating was a considerable challenge. There are indications from both features and finds of activity predating the main set of buildings exposed so far by excavation. Forty-six dates on 39 samples are presented and are interpreted in a formal chronological framework. Two models are presented, reflecting different possible readings of the sequence. Both indicate that piered architecture was in use by the thirtieth century cal BC and that the massive Structure 10, not the first building in the sequence, was also in existence by the thirtieth century cal BC. Activity associated with piered architecture came to an end (in Model 2) around 2800 cal BC. Midden and rubble infill followed. After an appreciable interval, the hearth at the centre of Structure 10 was last used around 2500 cal BC, perhaps the only activity in an otherwise abandoned site. The remains of some 400 or more cattle were deposited over the ruins of Structure 10: in Model 2, in the mid-twenty-fifth century cal BC, but in Model 1 in the late twenty-fourth or twenty-third century cal BC. The chronologies invite comparison with the near-neighbour of Barnhouse, in use from the later thirty-second to the earlier twenty-ninth century cal BC, and the Stones of Stenness, probably erected by the thirtieth century cal BC. The Ness, including Structure 10, appears to have outlasted Barnhouse, but probably did not endure as long in its primary form as previously envisaged. The decay and decommissioning of the Ness may have coincided with the further development of the sacred landscape around it; but precise chronologies for other sites in the surrounding landscape are urgently required. The spectacular feasting remains of several hundred cattle deposited above Structure 10 may belong to a radically changing world, coinciding (in Model 2) with the appearance of Beakers nationally, but it was arguably the by now mythic status of that building which drew people back to it.We are very grateful to many institutions and individuals, in particular: Ness of Brodgar Trust, Foundation for World Health, Orkney Islands Council, University of the Highlands and Islands, Orkney Archaeology Society, American Friends of the Ness of Brodgar, Northlink, Talisman- Sinopec, Hiscox Insurance, Historic Environment Scotland, and numerous other supporters and volunteers; Mark Edmonds, Ann MacSween, Colin Richards, and Alison Sheridan for encouragement, advice, and critical comments on an earlier draft of this article; three anonymous referees for their comments; and Kirsty Harding for help with the figures. Dating and modelling have been supported by a European Research Council Advanced Investigator Grant (295412), The Times of Their Lives (www.totl.eu), led by Alasdair Whittle and Alex Bayliss

A gene expression fingerprint of C. elegans embryonic motor neurons

BACKGROUND: Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo. RESULTS: Fluorescence Activated Cell Sorting (FACS) was used to isolate unc-4::GFP neurons from primary cultures of C. elegans embryonic cells. Microarray experiments detected 6,217 unique transcripts of which ~1,000 are enriched in unc-4::GFP neurons relative to the average nematode embryonic cell. The reliability of these data was validated by the detection of known cell-specific transcripts and by expression in UNC-4 motor neurons of GFP reporters derived from the enriched data set. In addition to genes involved in neurotransmitter packaging and release, the microarray data include transcripts for receptors to a remarkably wide variety of signaling molecules. The added presence of a robust array of G-protein pathway components is indicative of complex and highly integrated mechanisms for modulating motor neuron activity. Over half of the enriched genes (537) have human homologs, a finding that could reflect substantial overlap with the gene expression repertoire of mammalian motor neurons. CONCLUSION: We have described a microarray-based method, MAPCeL, for profiling gene expression in specific C. elegans motor neurons and provide evidence that this approach can reveal candidate genes for key roles in the differentiation and function of these cells. These methods can now be applied to generate a gene expression map of the C. elegans nervous system

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field