1,566 research outputs found

    Chlamydia Hijacks ARF GTPases To Coordinate Microtubule Posttranslational Modifications and Golgi Complex Positioning.

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    The intracellular bacterium Chlamydia trachomatis develops in a parasitic compartment called the inclusion. Posttranslationally modified microtubules encase the inclusion, controlling the positioning of Golgi complex fragments around the inclusion. The molecular mechanisms by which Chlamydia coopts the host cytoskeleton and the Golgi complex to sustain its infectious compartment are unknown. Here, using a genetically modified Chlamydia strain, we discovered that both posttranslationally modified microtubules and Golgi complex positioning around the inclusion are controlled by the chlamydial inclusion protein CT813/CTL0184/InaC and host ARF GTPases. CT813 recruits ARF1 and ARF4 to the inclusion membrane, where they induce posttranslationally modified microtubules. Similarly, both ARF isoforms are required for the repositioning of Golgi complex fragments around the inclusion. We demonstrate that CT813 directly recruits ARF GTPases on the inclusion membrane and plays a pivotal role in their activation. Together, these results reveal that Chlamydia uses CT813 to hijack ARF GTPases to couple posttranslationally modified microtubules and Golgi complex repositioning at the inclusion.IMPORTANCEChlamydia trachomatis is an important cause of morbidity and a significant economic burden in the world. However, how Chlamydia develops its intracellular compartment, the so-called inclusion, is poorly understood. Using genetically engineered Chlamydia mutants, we discovered that the effector protein CT813 recruits and activates host ADP-ribosylation factor 1 (ARF1) and ARF4 to regulate microtubules. In this context, CT813 acts as a molecular platform that induces the posttranslational modification of microtubules around the inclusion. These cages are then used to reposition the Golgi complex during infection and promote the development of the inclusion. This study provides the first evidence that ARF1 and ARF4 play critical roles in controlling posttranslationally modified microtubules around the inclusion and that Chlamydia trachomatis hijacks this novel function of ARF to reposition the Golgi complex

    Nondestructive SEM for surface and subsurface wafer imaging

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    The scanning electron microscope (SEM) is considered as a tool for both failure analysis as well as device characterization. A survey is made of various operational SEM modes and their applicability to image processing methods on semiconductor devices

    Transcriptomes of parents identify parenting strategies and sexual conflict in a subsocial beetle

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    This work was funded by UK NERC grants to M.G.R. and A.J.M. an NERC studentship to D.J.P. the University of Georgia and a US NSF grant to A.J.M. and M.G.R.Parenting in the burying beetle Nicrophorus vespilloides is complex and, unusually, the sex and number of parents that can be present is flexible. Such flexibility is expected to involve specialized behaviour by the two sexes under biparental conditions. Here, we show that offspring fare equally well regardless of the sex or number of parents present. Comparing transcriptomes, we find a largely overlapping set of differentially expressed genes in both uniparental and biparental females and in uniparental males including vitellogenin, associated with reproduction, and takeout, influencing sex-specific mating and feeding behaviour. Gene expression in biparental males is similar to that in non-caring states. Thus, being ‘biparental’ in N. vespilloides describes the family social organization rather than the number of directly parenting individuals. There was no specialization; instead, in biparental families, direct male parental care appears to be limited with female behaviour unchanged. This should lead to strong sexual conflict.Publisher PDFPeer reviewe

    Chandra Observations of the Disruption of the Cool Core in Abell 133

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    We present the analysis of a Chandra observation of the galaxy cluster Abell 133, which has a cooling flow core, a central radio source, and a diffuse, filamentary radio source which has been classified as a radio relic. The X-ray image shows that the core has a complex structure. The most prominent feature is a "tongue" of emission which extends from the central cD galaxy to the northwest and partly overlaps the radio relic. One possibility is that this tongue is produced by Kelvin-Helmholtz (KH) instabilities through the interaction between the cold gas around the cD galaxy and hot intracluster medium. We estimate the critical velocity and time scale for the KH instability to be effective for the cold core around the cD galaxy. We find that the KH instability can disrupt the cold core if the relative velocity is >~400 km s^-1. We compare the results with those of clusters in which sharp, undisrupted cold fronts have been observed; in these clusters, the low temperature gas in their central regions has a more regular distribution. In contrast to Abell 133, these cluster cores have longer timescales for the disruption of the core by the KH instability when they are normalized to the timescale of the cD galaxy motion. Thus, the other cores are less vulnerable to KH instability. Another possible origin of the tongue is that it is gas which has been uplifted by a buoyant bubble of nonthermal plasma that we identify with the observed radio relic. From the position of the bubble and the radio estimate of the age of the relic source, we estimate avelocity of ~700 km s^-1 for the bubble. The structure of the bubble and this velocity are consistent with numerical models for such buoyant bubbles. (abridged)Comment: 38 pages, 15 figures, accepted for publication in Ap

    Dynamic fracture in carbon-fibre composites: Air-blast loading

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    In this study a response of a 2x2 twill weave T300 carbon fibre/epoxy composite flat plate specimen resultant air blast dynamic response observed in is examined, using a combination of non-invasive analysis techniques. The study investigates deformation and damage following air blasts with incident pressures of 0.4 MPa, 0.6 MPa and 0.8 MPa, and wave speeds between 650m/s and 950m/s. Digital image correlation was employed to obtain displacement data from the rear surfaces of the specimens during each experiment. 3D x-ray tomography was used to visualize the resultant internal damage within the samples. It was shown that the global deformation and transitions in curvature of each specimen appear to be similar with varying out-of-plane displacements. Damage was observed to propagate from the rear surface of the specimens through to the front surface as the air blast magnitude increased

    A Chandra Observation of Abell 13: Investigating the Origin of the Radio Relic

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    We present results from the Chandra X-ray observation of Abell 13, a galaxy cluster that contains an unusual noncentral radio source, also known as a radio relic. This is the first pointed X-ray observation of Abell 13, providing a more sensitive study of the properties of the X-ray gas. The X-ray emission from Abell 13 is extended to the northwest of the X-ray peak and shows substructure indicative of a recent merger event. The cluster X-ray emission is centered on the bright galaxy H of Slee et al. 2001. We find no evidence for a cooling flow in the cluster. A knot of excess X-ray emission is coincident with the other bright elliptical galaxy F. This knot of emission has properties similar to the enhanced emission associated with the large galaxies in the Coma cluster. With these Chandra data we are able to compare the properties of the hot X-ray gas with those of the radio relic from VLA data, to study the interaction of the X-ray gas with the radio emitting electrons. Our results suggest that the radio relic is associated with cooler gas in the cluster. We suggest two explanations for the coincidence of the cooler gas and radio source. First, the gas may have been uplifted by the radio relic from the cluster core. Alternatively, the relic and cool gas may have been displaced from the central galaxy during the cluster merger event.Comment: 11 pages, 9 figures, Accepted for Publication in the Astrophysical Journal, higher-resolution figures can be found at http://www.astro.virginia.edu/~amj3r/Abell13

    The Production of Antibody by Invading B Cells Is Required for the Clearance of Rabies Virus from the Central Nervous System

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    Every year over 50,000 people die from rabies worldwide, primarily due to the poor availability of rabies vaccine in developing countries. However, even when vaccines are available, human deaths from rabies occur if exposure to the causative virus is not recognized and vaccination is not sought in time. This is because rabies virus immunity induced by the natural infection or current vaccines is generally not effective at removing disease-causing rabies virus from brain tissues. Our studies provide insight into why this is the case and how vaccination can be changed so that the immune response can clear the virus from brain tissues. We show that the type of immune response induced by a live-attenuated rabies virus vaccine may be the key. In animal models, live-attenuated rabies virus vaccines are effective at delivering the immune cells capable of clearing the virus into CNS tissues and promote recovery from a rabies virus infection that has spread to the brain while conventional vaccines based on killed rabies virus do not. The production of rabies-specific antibody by B cells that invade the CNS tissues is important for complete elimination of the virus. We hypothesize that similar mechanisms may promote rabies virus clearance from individuals who are diagnosed after the virus has reached, but not extensively spread, through the CNS

    The young age of the extremely metal-deficient blue compact dwarf galaxy SBS 1415+437

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    We use Multiple Mirror Telescope (MMT) spectrophotometry and Hubble Space Telescope (HST) Faint Object Spectrograph (FOS) spectra and Wide Field and Planetary Camera 2 (WFPC2) V and I images to study the properties and evolutionary status of the nearby (D = 11.4 Mpc) extremely metal-deficient blue compact dwarf (BCD) galaxy SBS 1415+437=CG 389. The oxygen abundance in the galaxy is 12+log(O/H)=7.60+/-0.01 or Zsun/21. The helium mass fraction in SBS 1415+437 is Y=0.246+/-0.004 which agrees with the primordial helium abundance determined by Izotov & Thuan using a much larger sample of BCDs. The alpha-elements-to-oxygen abundance ratios (Ne/O, S/O, Ar/O) are in very good agreement with the mean values for other metal-deficient BCDs and are consistent with the scenario that these elements are made in massive stars. The Fe/O abundance ratio is ~2 times smaller than the solar ratio. The Si/O ratio is close to the solar value, implying that silicon is not significantly depleted into dust grains. The values of the N/O and C/O ratios imply that intermediate-mass stars have not had time to evolve in SBS 1415+437 and release their nucleosynthesis products and that both N and C in the BCD have been made by massive stars only. This sets an upper limit of ~100 Myr on the age of SBS 1415+437. The (V-I) color of the low-surface-brightness component of the galaxy is blue (<0.4 mag) indicative of a very young underlying stellar population. The (V-I) - I color-magnitude diagrams of the resolved stellar populations in different regions of SBS 1415+437 suggest propagating star formation from the NE side of the galaxy to the SW. All regions in SBS 1415+437 possess very blue spectral energy distributions (SED). We find that the ages of the stellar populations in SBS 1415+437 to range from a few Myr to 100 Myr.Comment: 25 pages, 12 PS and 5 JPG figures, to appear in Ap
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