125 research outputs found
Heat Exchangers
Tato bakalĂĄĆskĂ© prĂĄce mĂĄ seznĂĄmit ÄtenĂĄĆe se zĂĄkladnĂmi druhy vĂœmÄnĂkĆŻ tepla pouĆŸĂvanĂœmi v energetice a jejich rozdÄlenĂm. Je zde uvedeno jejich tĆĂdÄnĂ jak z hlediska ĂșÄelu a funkce, tak i z hlediska konstrukÄnĂho, kterĂ© je doplnÄno porovnĂĄnĂm rekuperaÄnĂch vĂœmÄnĂkĆŻ. DĂĄle se zabĂœvĂĄ moĆŸnostmi zvyĆĄovĂĄnĂ ĂșÄinnosti, problĂ©my pĆi provozu a jejich odstraĆovĂĄnĂm. ZejmĂ©na pak zanĂĄĆĄenĂm a ÄiĆĄtÄnĂm vĂœmÄnĂkĆŻ tepla.This bachelor thesis shall inform readers about basis types of heat exchangers used in power industry and their basis sorting. They are sorted not only from the point of view of their purpose and function, but also, from the point of view of their construction. Apart from that, there is a comparison of recuperative heat exchangers. Moreover, the thesis deals with possibilities to enhance their efficiency, possible problems in service and ways to solve these problems, maily with sedimentation and cleaning heat exchangers.
Brush Steam Turbine
DiplomovĂĄ prĂĄce se zabĂœvĂĄ provozem kartĂĄÄovĂ© parnĂ turbĂny instalovanĂ© v malĂ© biomasovĂ© spalovnÄ v areĂĄlu firmy DĆevopar v LoĆĄticĂch. Ăvod prĂĄce je zamÄĆen na pĆedstavenĂ technologie kotelny. NĂĄsledujĂcĂ ÄĂĄsti jsou zamÄĆeny na princip, konstrukÄnĂ ĆeĆĄenĂ a vyhodnocenĂ provozu kartĂĄÄovĂ© turbĂny. ParnĂ turbĂny pro srovnĂĄnĂ jsou voleny podle pĆedbÄĆŸnĂ©ho vĂœpoÄtu. ZĂĄvÄr prĂĄce obsahuje porovnĂĄnĂ kartĂĄÄovĂ© turbĂny s lopatkovou.This thesis deals with brush steam turbine operation. This turbine is installed in small biomass incineration plant, which is situated in premises of company DĆevopar s.r.o. in LoĆĄtice. First part is focused on incineration plant technology introduction. Following parts are focused on brush steam turbine principle, design solutions and evaluation of the operation. Comparative steam turbines are chosen according to preliminary calculation. The end of thesis contains brush turbine and blade turbine comparison.
First report of venereal and vertical transmission of canine leishmaniosis from naturally infected dogs in Germany
<p>Abstract</p> <p>Background</p> <p>Canine leishmaniosis (CanL) is a zoonotic disease caused by <it>Leishmania (L.) infantum</it>. It is endemic to several tropical and subtropical countries but also to the Mediterranean region. It is transmitted by phlebotomine sandflies but occasional non-vector transmissions have been reported, including vertical and horizontal transmission.</p> <p>Findings</p> <p>The authors report a case of CanL in a female boxer dog from Dusseldorf, Germany, that had never been in an endemic region. A serum sample from the bitch was tested positive for antibodies against <it>Leishmania </it>(IFAT 1:2,000, ELISA 72). The bitch had whelped three litters, and one puppy from the third litter was also found to be seropositive for <it>Leishmania </it>antibodies (IFAT 1:4,000, ELISA 78).</p> <p>Conclusions</p> <p>Up to now, despite intensive searching, the occurrence of sandflies could not be proved in the bitch's region of origin. Thus, vertical and horizontal transmission are to be discussed as possible ways of infection. This may be the first report of venereal and vertical transmission of <it>L. infantum </it>in naturally infected dogs in Germany.</p
Serological survey of Leishmania infantum and Trypanosoma cruzi in dogs from urban areas of Brazil and Colombia
Leishmania infantum and Trypanosoma cruzi are zoonotic parasites that are endemic throughout many parts of Latin America. Infected dogs play an important role in transmission of both parasites to humans. A serological survey of Leishmania and Trypanosoma infection was conducted on 365 dogs from SĂŁo Paulo, Brazil and BogatĂĄ, Colombia, South America. Serum samples were examined by the indirect immunofluorescent antibody test (IFAT). Anti-Leishmania IgG antibodies were detected in 5 of 107 from Brazil (4.7 %) and in 4 of 258 dogs (1.6%) from Colombia. Titers ranged from 1:25 to 1:100. Anti-T. cruzi antibodies were not detected in any of the dogs from either Brazil or Colombia. The results show a low prevalence of anti-Leishmania antibodies and no antibodies against T. cruzi in these canine populations. Our study suggests that dogs play a limited role in the spread of L. infantum and T. cruzi in these urban areas of Brazil and Colombia
Evaluation of rK39 rapid diagnostic tests for canine visceral leishmaniasis : longitudinal study and meta-analysis
Canine visceral leishmaniasis is a vector-borne disease caused by the intracellular parasite Leishmania infantum. It is an important veterinary disease, and dogs are also the main animal reservoir for human infection. The disease is widespread in the Mediterranean area, and parts of Asia and South and Central America, and is potentially fatal in both dogs and humans unless treated. Diagnosis of canine infections requires serological or molecular tests. Detection of infection in dogs is important prior to treatment, and in epidemiological studies and control programmes, and a sensitive and specific rapid diagnostic test would be very useful. Rapid diagnostic tests (RDTs) have been developed, but their diagnostic performance has been reported to be variable. We evaluated the sensitivity of a RDT based on serological detection of the rK39 antigen in a cohort of naturally infected Brazilian dogs. The sensitivity of the test to detect infection was relatively low, but increased with time since infection and the severity of infection. We then carried out a meta-analysis of published studies of rK39 RDTs, evaluating the sensitivity to detect disease and infection. The results suggest that rK39 RDTs may be useful in a veterinary clinical setting, but the sensitivity to detect infection is too low for operational control programmes
Transplacental Transmission of Leishmania infantum as a Means for Continued Disease Incidence in North America
Dogs are a favored feeding source for sand flies that transmit human L. infantum infection. Zoonotic visceral leishmaniasis (ZVL) is an emerging problem in some U.S. dog breeds, with over 20% of at-risk Foxhounds infected. Although classically Leishmania is transmitted by infected sand flies which exist in the United States, no role has yet been determined for vector-borne transmission. Means of ongoing L. infantum transmission in U.S. dogs is unknown. Possibilities include transplacental and horizontal/venereal transmission. Aims for this study were to establish whether transplacental transmission occurred in Leishmania-infected U.S. dogs and determine the effect of this transmission on immune recognition of Leishmania. This novel report describes wide-spread infection as identified by kqPCR in 8 day-old pups born to a naturally-infected, seropositive U.S. dog with no travel history. This is the first report of transplacental transmission of L. infantum in naturally-infected dogs in North America. Evidence that mom-to-pup transmission of ZVL may continue disease in an otherwise non-endemic region has significant implications on current control strategies for ZVL. Determining frequency of vertical transmission and incorporating canine sterilization with vector control may have a more significant impact on ZVL transmission to people in endemic areas than current control efforts
Quantification of Leishmania infantum DNA in females, eggs and larvae of Rhipicephalus sanguineus
<p/> <p>Background</p> <p><it>Leishmania infantum </it>is a widespread parasite that affects dogs and humans worldwide. It is transmitted primarily by phlebotomine sand flies, but recently there has been much discussion on the role of the brown dog tick, <it>Rhipicephalus sanguineus</it>, as a potential vector for this protozoan. Recent laboratory and field investigations have contributed to this hypothesis, but a proof of the vector capacity of <it>R. sanguineus </it>has yet to be provided. Following a recent study suggesting that <it>L. infantum </it>passes transovarially from the female tick to her progeny the current study provides new evidence of the transovarial transmission of <it>L. infantum </it>in <it>R. sanguineus</it>.</p> <p>Methods</p> <p>Engorged females of <it>R. sanguineus </it>were collected from the environment in a dog shelter of southern Italy, where canine leishmaniosis is endemic. In the laboratory, 97 females that successfully laid eggs, their eggs and the originated larvae were subjected to DNA extraction and then tested by a TaqMan-based real time PCR targeting a fragment of the kinetoplast DNA (kDNA) of <it>L. infantum</it>.</p> <p>Results and conclusions</p> <p><it>L. infantum </it>kDNA was detected in engorged females, their eggs and originating larvae, with a parasite load ranging from 1.8 Ă 10<sup>-4 </sup>to 10.0 Ă 10<sup>0</sup>. Certainly, the current study provides further evidence on the passage of <it>L. infantum </it>from <it>R. sanguineus </it>females to their offspring. The observation of promastigote forms in larvae is necessary to definitively confirm this hypothesis, which would raise interesting questions about the possible role of ticks in the maintenance of <it>L. infantum </it>infection among dogs in certain areas.</p
Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil
Detection of Leishmania infantum DNA mainly in Rhipicephalus sanguineus male ticks removed from dogs living in endemic areas of canine leishmaniosis
Background: Sand flies are the only biologically adapted vectors of Leishmania parasites, however, a possible role in the transmission of Leishmania has been proposed for other hematophagous ectoparasites such as ticks. In order to evaluate natural infection by Leishmania infantum in Rhipicephalus sanguineus ticks, taking into account its close association with dogs, 128 adult R. sanguineus ticks removed from 41 dogs living in endemic areas of canine leishmaniosis were studied. Methods: Individual DNA extraction was performed from each tick and whole blood taken from dogs. Dog sera were tested for IgG antibodies to L. infantum antigen by ELISA and L. infantum real-time PCR was performed from canine whole blood samples and ticks. Results: Leishmania infantum PCR was positive in 13 ticks (10.1%) including one female, (2.0%) and 12 males (15.2%), and in only five dogs (12.2%). Male ticks had a significantly higher infection rate when compared to female R. sanguineus. The percentage of L. infantum seroreactive dogs was 19.5%. All but two PCR positive dogs were seroreactive. Leishmania infantum PCR positive ticks were removed from seropositive and seronegative dogs with a variety of PCR results. Conclusions: This study demonstrates high prevalence of L. infantum DNA in R. sanguineus ticks removed from L. infantum seropositive and seronegative dogs. The presence of L. infantum DNA was detected mainly in male ticks possibly due to their ability to move between canine hosts and feed on several canine hosts during the adult life stage. Additional studies are needed to further explore the role of R. sanguineus ticks and in particular, male adults, in both the epidemiology and immunology of L. infantum infection in dogs in endemic areas
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