Perfil glicêmico e lipídico em meninos de diferentes classes sociais

Estudos sugerem que o nível socioeconômico (NSE) exerce influência no distúrbio metabólico, desta forma o presente estudo visou verificar as diferenças do perfil glicêmico e lipídico em meninos de diferentes classes sociais da cidade de Curitiba, Paraná. A amostra constituiu-se de 123 meninos (14,0±2,2 anos) sendo avaliado o perfil antropométrico, NSE, perfil glicêmico e lipídico. A análise estatística foi descritiva e para verificar as diferenças entre os níveis foi utilizado a ANOVA one-way com post hoc de Bonferroni com p< 0,05. Meninos de médio e alto NSE apresentaram maiores valores médios de glicemia em jejum, triglicerídeos (TG) e LDL-c do que indivíduos de baixo NSE (

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure &lt;= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Aspectos relacionados à qualidade de sementes de coentro Aspects related to coriander seed quality

O coentro é uma hortaliça amplamente consumida no Brasil como condimento, de grande importância socio-econômica. Problemas relacionados ao baixo vigor de sementes e ao estabelecimento da cultura são uma constante nesta espécie. No presente trabalho 11 lotes comerciais de sementes de coentro das cultivares Verdão, Americano Gigante, Tapacurá, Tabocas, Palmeira e Asteca foram analisados quanto a pureza, germinação, vigor e qualidade sanitária. Em outro estudo, avaliaram-se os efeitos das temperaturas de 15; 20; 25; 30 e 35ºC sobre a germinação das sementes das mesmas cultivares do experimento anterior e mais a cultivar Verdão HV. Houve variação relativamente pequena da germinação entre os lotes e com valores superiores ao padrão nacional para comercialização de sementes. Apenas o lote 1 apresentou pureza abaixo do referido padrão. A qualidade das sementes de coentro comercializadas pelas empresas de sementes no país é, geralmente, satisfatória. Quanto à sanidade, foi detectada a presença de alguns fungos de importância para a cultura do coentro, como Alternaria alternata e Alternaria radicina, com índices de associação elevados. Em um lote, foi detectada a presença de Alternaria dauci. Verificou-se efeito significativo da temperatura, de cultivar e da interação entre a cultivar e temperatura na germinação das sementes. Temperaturas entre 15 e 25ºC proporcionaram maior germinação, enquanto as temperaturas mais elevadas foram prejudiciais. As cultivares 'Verdão HV' e 'Palmeira' germinaram satisfatoriamente a 30ºC. Praticamente não ocorreu germinação a 35ºC para todas as cultivares avaliadas.<br>Coriander is a vegetable crop with large consumption in Brazil, of high social and economic importance. Problems related to low seed vigor and cultivation are reported in this crop. In the present study, eleven seed lots of coriander cultivars 'Verdão', 'Americano Gigante', 'Tapacurá', 'Tabocas', 'Palmeira' and 'Asteca' were used to determine the purity, germination, vigor and pathological seed quality. In another study, effects on seed germination of the same commercial cultivars and 'Verdão HV' were evaluated at temperatures of 15; 20; 25; 30 and 35ºC. Little variation in the germination was observed among seed lots. All lots exhibited germination above the national standard, and lot 1 presented purity below the standard for seed trading. Purity and germination of coriander seeds in Brazil is generally, satisfactory. Regarding to pathological seed quality, some important fungi of coriander crop were detected, as Alternaria alternata and Alternaria radicina. In one lot was detected Alternaria dauci. Effects of temperature, cultivar and an interaction effect between cultivar and temperature on seed germination was observed. Temperatures between 15 and 25ºC were favorable to germination whereas higher temperatures reduced the germination. The cultivars 'Verdão HV' and 'Palmeira' germinated at 30ºC. At 35ºC no germination was observed in all tested cultivars, possibly due to thermoinhibition, since the seeds germinated when transferred to 20ºC

Familial Analysis of Seropositivity to Trypanosoma cruzi and of Clinical Forms of Chagas Disease

A cross-sectional study was carried out in Água Comprida, MG, Brazil, a region previously endemic to Chagas disease whose vectorial transmission was interrupted around 20 year ago. A total of 998 individuals were examined for anti-Trypanosoma cruzi antibodies. Seropositivity was observed in 255 subjects (25.5%), and 743 subjects were negative. Forty-one families with 5–80 individuals with similar environmental conditions were selected for familial analysis. In 15 families, seropositivity to T. cruzi was observed in > 50% of individuals. The segregation analysis confirmed family aggregation for the seropositivity to the T. cruzi. Heart commitment was the major clinical form observed, and in six families, > 50% of the individuals display cardiopathy that may be attributed to T. cruzi infection. Our results support the hypothesis that there is a family aggregation for the seropositivity but without the effect of one major gene

Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime

Federal University of Pará. Postgraduate Program in Biology of Infectious & Parasitic Agents. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório der Microscopia Eletrônica. Ananindeua, PA, Brasil.Escola Superior da Amazônia. Department of Biomedicine. Belém, PA, Brazil.Federal University of Pará,. Department of Mechanical Engineering. Belém, PA, Brazil.Federal University of Pará. Faculty of Chemical Engineering. Belém, PA, Brazil.Federal University of Pará. Physico-Chemical Drug Quality Control Laboratory. Belém, PA, Brazil.Federal University of Pará,. Department of Mechanical Engineering. Belém, PA, Brazil.Federal University of Pará. Physico-Chemical Drug Quality Control Laboratory. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório der Microscopia Eletrônica. Ananindeua, PA, Brasil.Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis. Materials & methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated. Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite1, nanocomposite2 and nanocomposite3, respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite2 and nanocomposite3, respectively. Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo