Sustainable Drying Technologies for the Development of Functional Foods and Preservation of Bioactive Compounds

Nowadays, the sustainability of a product, a process or a system is assessed according to three dimensions: environmental, social and economic. Sustainability challenges occur at all stages in the food system from production through processing, distribution and retailing to consumption and waste disposal. The promotion of organic and local food is not the only way to reach the sustainability. There is other possibility that implies to continue the production hegemony. Increasing research is being focused on the development of healthy, quality and safety food products adapted to consumer’s needs and more environment-friendly processes, that is, processes consuming energy more efficiently, generating less waste and emitting less greenhouse effect gases. Drying technology is applied in the food industry not only for preservation but also to manufacture foods with certain characteristics. Drying technology operations need to be precisely controlled and optimized in order to produce a good-quality product with the highest level of nutrient retention and flavor together with microbial safety. This chapter contains detailed information about some measurements taken by the food industry to ensure the supply of bioactive nutrients to as many individuals as possible, assuring the global sustainability. More specifically, the contribution of some drying techniques employed in the development of functional foods to increase the sustainability of the feeding process is discussed

Effects of pulsed electric field-assisted osmotic dehydration and edible coating on the recovery of anthocyanins from in vitro digested berries

Berry fruits, such as strawberries and blueberries, are rich sources of anthocyanins. Several studies have been made on the impact of non-thermal treatments on safety, shelf-life and nutritional characteristics of such products, but the effects of these processes on anthocyanin stability during digestion in the gastrointestinal tract are still not completely clear. The aim of this study was to assess the recovery of anthocyanins after simulated gastrointestinal digestion of (1) strawberry samples, pre-treated with pulsed electric field (PEF) at 100 or 200 V\ub7cm−1, prior to osmotic dehydration (OD), and (2) blueberry samples coated with chitosan and procyanidin. After digestion, a significantly higher content of cyanidin-3-O-glucoside and malvidin-3-O-glucoside was quantified by LC-MS/MS in processed strawberry and blueberry samples, compared with the controls. The highest recovery of cyanidin-3-O-glucoside was detected in digested strawberry samples osmotically dehydrated with trehalose. The recovery of malvidin-3-O-glucoside was highest in digested blueberries coated with chitosan and stored for 14 days, compared with untreated samples or samples coated with chitosan and procyanidin. Our study shows the potential of mild PEF treatments combined with OD, or the use of edible coating, to obtain shelf-stable products without substantially affecting the composition or the stability of anthocyanins during digestion in the upper gastrointestinal tract

Food science and technology students self-evaluate soft and technical skills

Food Scientists and Technologists (FS&T) need diverse skills in the globalized food and drink sector: Food-specific or scientific / technical skills and generic or intuitive soft skills. This study determined how satisfied FS&T students were with overall improvement, and in key technical and soft skills, based on their university work; and if satisfaction was linked to geography, degree in progress, anticipated degree, anticipated work place or anticipated job responsibility. An on-line survey was completed by 267 students in over 20 countries using a 5-point Likert scale to evaluate satisfaction. Responses were analyzed by the Friedman or Kruskal Wallis tests for more than two groups, otherwise by the Wilcoxon Signed Rank or Mann-Whitney tests. There were no differences in Overall Satisfaction with technical and soft skills training. Among soft skills, training in Working with Others and Being Responsible were more often rated “Excellent” and students were more satisfied with their training than with Solving Problems, Communication and Positive Attitude. Students anticipating a job with high responsibility were more satisfied with overall soft skill training and with 3 of the 5 specific soft skills. Among technical skills, students were more satisfied with improvement in basic sciences (Microbiology, Chemistry, Processing, Safety), and those in Northern Europe were more satisfied with overall technical training. These data show variations in perception and/or efficacy of technical and soft skill training in Food Science programmes and underline the need for separate attention to the incorporation of soft skill training into the design of FS&T coursesinfo:eu-repo/semantics/publishedVersio

Selected chemical and physico-chemical properties of ultrasound-assisted osmodehydrated kiwifruit.

Ultrasounds are air vibrations and they generate a rapid series of compression and expansion, having an increasing effect on mass diffusion when combined with osmotic dehydration process (OD). The aim of this work was to investigate the effect of ultrasound (US) pretreatment on the texture, color, chlorophyll content, water activity and thermal properties in terms of freezable water content of osmotic dehydrated kiwifruit. Kiwifruits slices (10 mm thick) were subjected to ultrasonic waves in the bath at a frequency of 35 kHz for 10, 20 and 30 minutes. The osmotic dehydration was carried out by immersion of the samples in 61.5% sucrose solution equilibrated at 25°C for pre-established contact period of 0, 10, 20 and 30 min. The results showed that ultrasound pre-treatment by itself caused a lowering of the kiwifruit firmness in comparison with untreated sample. However 120 minutes after OD treatment the increase of the firmness parameter occurred, which was associated with a greater loss of water during the osmotic dehydration. Moreover there was noticed almost unchanged chlorophyll content in kiwifruit treated with ultrasound compared to fresh sample. During all OD processes the loss of chlorophyll content was observed, however lower for the US pre-treated samples. These data were in agreement with the colour changes observed in kiwifruit samples. Similarly, with increasing time of OD process a decrease of water activity and freezable water content in kiwifruit tissue was observed. In conclusion it can be said that the application of US pre-treatment has a positive effect on different kiwifruit characteristics, particularly in colour and chlorophyll retention

Simple and efficient approach for shelf-life test on frozen spinach and parsley

open6noA simple test for shelf-life assessment of frozen spinach and parsley is presented. A specific shelf-life test that considers three storage temperatures is proposed to accelerate the rate of quality decay in frozen spinach and parsley. The scope was to provide a reliable and rapid way (one month vs years) to predict shelf-life by using a simple experimental approach and mathematical models based on some physical quality product attributes. Physical properties were evaluated at three storage temperatures: -5 degrees C, -10 degrees C and -26 degrees C, to simulate a possible thermal abuse. Mechanical and thermal indexes were defined measuring maximum compression force (N) and latent heat involved in ice melting (J/g). A zeroorder kinetic model was used to properly fit experimental data and thus to obtain related reaction rates. The determination coefficient indicates that there is a strong linear relation between kinetic parameters at -10 degrees C or -5 degrees C and -26 degrees C. This suggests a reliable procedure for shelf-life estimation, carrying out a test at -10 degrees C or -5 degrees C for one month and extending values to data acquired at 26 degrees C for the same period of time. The relations obtained from this research have led to a simple practical approach: one day at -10 degrees C could be considered roughly equivalent to 30 days at -26 degrees C. Accordingly, it could be possible to obtain a shelf-life estimation in short time, also considering other similar products.openIaccheri, Eleonora; Cevoli, Chiara; Romani, Santina; Dalla Rosa, Marco; Molari, Giovanni; Fabbri, AngeloIaccheri, Eleonora; Cevoli, Chiara; Romani, Santina; Dalla Rosa, Marco; Molari, Giovanni; Fabbri, Angel

Valutazione delle caratteristiche fisiche e strutturali di un prodotto da forno durante la conservazione (Spianata di Ozieri)

The aim of this research was the characterization by physical and structural properties, a typical bakery product (Spianata di Ozieri). The samples of two differents kind of spianata were submitted to dynamometric analysis (texture profile analysis - TPA, cut and tension test), color measurement and microbiological tests over 14 days of storage time at 22°C. Three samplings were conducted in different periods of the year to test the seasonality of the production. A collaborative test was carried out at the DISAABA (Sassari University) and in the laboratories of Food Science & Technology (Bologna University). The most important results obtained in this research confirm effectivement that this kind of product is really typical and so the data variability obtained is quite normal. The microciological tests showed a good quality of the product during the normal storage time. Lo scopo di questa ricerca è stato quello di effettuare una prima caratterizzazione fisica e strutturale di un prodotto da forno tipico sardo, la spianata di Ozieri. In particolare, sono state studiate le variazioni di alcune caratteristiche strutturali dinamometriche, fisiche e chimico-fisiche e l'evoluzione della carica microbica durante la conservazione, a temperatura controllata di 22°C circa, di due diversi campioni commerciali di spianata sarda, realizzati artigianalmente e confezionati con metodologie diverse: uno in atmosfera ordinaria e destinato al mercato locale e l'altro confezionato in atmosfera protettiva (40% anidride carbonica, 60% azoto) e destinato alla grande distribuziooe. Sono stati effettuati tre campionamenti nei mesi di luglio, novembre e gennaio, per valutare eventuali differenze dovute alia stagionalità di produzione. Le prove sono state condotte in collaborazione tra i laboratori del Disaaba dell'Università di Sassari e del Corso di Laurea in Scienze e Tecnologie Alimentari di Cesena, Università di Bologna. Sono stati analizzati i campioni delle due differenti produzioni e dei tre diversi campionamenti stagionali, valutando le modificazioni delle caratteristiche fisiche in conservazione, mentre l'evoluzione della carica microbica è stata valutata sulla campionatura di gennaio. Si riporteranno di seguito, i risultati ottenuti durante il periodo di osservazione, della durata di 14 giorni per campioni confezionati in atmosfera ordinaria e di 27 giomi per quelli condizionati in atmosfera protettiva

Modification of Transverse NMR Relaxation Times and Water Diffusion Coefficients of Kiwifruit Pericarp Tissue Subjected to Osmotic Dehydration

The objective of the present study was to evaluate cellular compartment modifications of kiwifruit (Actinidia deliciosa) outer pericarp tissue caused by osmotic treatment in a 61. 5 % sucrose solution, through the quantification of transverse relaxation time (T2) and water self-diffusion coefficient (Dw) obtained by low field nuclear magnetic resonance means. Raw material ripening stage was taken into account as an osmotic dehydration (OD) process variable by analyzing two different kiwifruit groups, low (LB) and high (HB) °Brix. Three T2 values were obtained of about 20, 310, and 1,250 ms, which could be ascribed to the proton populations, located in the cell walls, in the cytoplasm/extracellular space, and in the vacuoles, respectively. According to T2 intensity values, vacuole protons represented between 47 and 60 % of the total kiwifruit protons, for LB and HB kiwifruits, respectively. The leakage of water leading to vacuole shrinkage seemed to cause concentration of solutes, retained by the tonoplast, making the vacuole T2 value decrease along the OD. As expected, the Dw values of raw kiwifruits were lower than the value of the free pure water. The water mobility (and hence Dw), depending on the kiwifruit distinctive cellular structures and solutes, decreased even more during OD due to water loss and sugar gain phenomena. Dw represents an average value of the diffusion coefficient of the whole kiwifruit tissue protons. In order to obtain Dw values specific for each cellular compartment, a multiple component model fitting was also used. According to these results, the vacuole water self-diffusion coefficient (Dw,v) was much higher than Dw.Fil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Università di Bologna; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laghi, Luca. Università di Bologna; ItaliaFil: Panarese, Valentina. Università di Bologna; ItaliaFil: Tylewicz, Urszula. Università di Bologna; ItaliaFil: Rocculi, Pietro. Università di Bologna; ItaliaFil: Dalla Rosa, Marco. Università di Bologna; Itali

Effect of drying process, encapsulation, and storage on the survival rates and gastrointestinal resistance of L. Salivarius spp. salivarius included into a fruit matrix

[EN] In a new probiotic food, besides adequate physicochemical properties, it is necessary to ensure a minimum probiotic content after processing, storage, and throughout gastrointestinal (GI) digestion. The aim of this work was to study the effect of hot air drying/freeze drying processes, encapsulation, and storage on the probiotic survival and in vitro digestion resistance of Lactobacillus salivarius spp. salivarius included into an apple matrix. The physicochemical properties of the food products developed were also evaluated. Although freeze drying processing provided samples with better texture and color, the probiotic content and its resistance to gastrointestinal digestion and storage were higher in hot air dried samples. Non-encapsulated microorganisms in hot air dried apples showed a 79.7% of survival rate versus 40% of the other samples after 28 days of storage. The resistance of encapsulated microorganisms to in vitro digestion was significantly higher (p <= 0.05) in hot air dried samples, showing survival rates of 50-89% at the last stage of digestion depending on storage time. In freeze dried samples, encapsulated microorganisms showed a survival rate of 16-47% at the end of digestion. The different characteristics of the food matrix after both processes had a significant effect on the probiotic survival after the GI digestion. Documented physiological and molecular mechanisms involved in the stress response of probiotic cells would explain these results.Authors thank the postdoctoral grant Juan de la Cierva Incorporacion (IJCI-2016-29679).Betoret, E.; Betoret Valls, N.; Calabuig-Jiménez, L.; Barrera Puigdollers, C.; Dalla Rosa, M. (2020). Effect of drying process, encapsulation, and storage on the survival rates and gastrointestinal resistance of L. Salivarius spp. salivarius included into a fruit matrix. 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In vitro evaluation of gastrointestinal survival of Lactobacillus amylovorus DSM 16698 alone and combined with galactooligosaccharides, milk and/or Bifidobacterium animalis subsp. lactis Bb-12. International Journal of Food Microbiology, 149(2), 152-158. doi:10.1016/j.ijfoodmicro.2011.06.010Ester, B., Noelia, B., Laura, C.-J., Francesca, P., Cristina, B., Rosalba, L., & Marco, D. R. (2019). Probiotic survival and in vitro digestion of L. salivarius spp. salivarius encapsulated by high homogenization pressures and incorporated into a fruit matrix. LWT, 111, 883-888. doi:10.1016/j.lwt.2019.05.088Burgain, J., Gaiani, C., Linder, M., & Scher, J. (2011). Encapsulation of probiotic living cells: From laboratory scale to industrial applications. Journal of Food Engineering, 104(4), 467-483. doi:10.1016/j.jfoodeng.2010.12.031Capela, P., Hay, T. K. C., & Shah, N. P. (2006). 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Uticaj osmotske dehidracije na kivi: rezultati multianalitičkog pristupa proučavanja strukture

This paper presents the results of the comparison of different analytical techniques (Differential Scanning Calorimetry - DSC, Low Field Nuclear Magnetic Resonance - LF-NMR, Light Microscopy - LM and Transmission Electron Microscopy – TEM) in order to evaluate the mass transfer, water status and cellular compartment modifications of the kiwifruit outer pericarp tissue during osmotic dehydration treatment (OD). Two kiwifruit species, A. deliciosa and A. chinensis were submitted to OD. OD was performed in a 61.5 % w/v sucrose solution at three different temperatures (25, 35 and 45 °C), with treatment time from 0 to 300 min. Peleg’s model highlighted that the main response differences between the two kiwifruit species occurred during the initial phase of the osmotic treatment. DSC parameters appeared to be sensitive to water and solid exchange between fruits and osmotic solution. LF-NMR proton T2 revealed the consequences of the water-solid exchange on the cell compartments, namely vacuole, cytoplasm plus extracellular space and cell wall. During OD, the reduction of the vacuole proton pool, detected by LF-NMR, suggested a shrinkage of such compartment, confirmed by LM. Cell walls of outer pericarp showed considerable changes in size, structure and stain uptake during OD observed at TEM. The proposed multianalytical approaches should enable better design of combined processing technologies permitting the evaluation of their effects on tissue response.U ovom radu su prikazani rezultati poređenja različitih analitičkih tehnika (Diferencijalna skeniranja Kalorimetrija – DSC, Niska Polje nuklearna magnetna rezonanca - LF-NMR, svetlost mikroskopije - LM i Prenos elektronsku mikroskopiju - TEM), u cilju određivanja prenosa mase, statusa vode i modifikacije ćelija tkiva perikarpa kivija tokom osmotske dehidratacije tretmana (OD). Dve sorte kivija su bile izložene osmotskom tretmanu, A. deliciosa i A. chinensis. Osmotsko sušenje obavljeno je u 61,5% rastvoru saharoze na tri različite temperature (25, 35 i 45°C), sa vremenom trajanja sušenja od 0 do 300 minuta. Pelegovim modelom naglašene su glavne razlike između dve sorte kivija koje se događaju tokom početne faze osmotskog tretmana. DSC parametri osetljivi su na izmenu vode i čvrste materije između voća i osmotskog rastvora. LF-NMR-om otkrivene su posledice razmene vode i čvrste materije na ćeliju, odnosno vakuole, citoplazmu sa vanćelijskim prostorom i ćelijski zid. Tokom OD, smanjenje vakuole protona bazena, otkrivena je LF-NMR, predložio skupljanja takvog odeljka, potvrđeno LM. Ćelijske zidove spoljne perikarpa su pokazali značajne promene u veličini, strukturi i mrlja uzimanja u toku OD posmatrano na sistem. Predloženi multianalitical pristupi treba da omogući bolji dizajn u kombinaciji obrade tehnologija dozvoljava procenu njihovog uticaja na tkivo odgovor.Fil: Dalla Rosa, Marco. Università di Bologna; ItaliaFil: Tylewicz, Urszula. Università di Bologna; ItaliaFil: Panarese, Valentina. Università di Bologna; ItaliaFil: Laghi, Luca. Università di Bologna; ItaliaFil: Pisi, Annamaria. Università di Bologna; ItaliaFil: Santagapita, Patricio Roman. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rocculi, Pietro. Università di Bologna; Itali