DIFABEL PENGAJAR BAHASA INGGRIS DARING DALAM BIDANG PENYUTRADARAAN. AZHAR HIBATULLAH : 156020065

Pengkaryaan ini membahas mengenai seorang difabel pengajar bahasa Inggris daring asal Nyalindung, Kabupaten Sukabumi, Jawa Barat. Sosok itu bernama Engkus,ia menjadi seorang tutor bahasa Inggris daring padalaman jejaring sosial facebookAyo Belajar Bahasa Inggris dari Nol (0) dengan materi pengajaran pengenalantenses. Penelitian ini di latarbelakangi karena kurangnya kepercayaan masyarakat terhadap metode pembelajaran bahasa Inggris yang diberikan oleh Engkus. Tujuan pengkaryaan ini untuk memberikan pengetahuan dan informasi kepada khalayak tentang potensi yang dimiliki seorang difabel yang menjadi pengajar bahasa Inggris daring. Fenomena inidiceritakan dan divisualisasikan dalam sebuah media yangmampu mendeskripsikan keadaan sebenarnya melalui film dokumenter yang bergenre ekspositori, Penelitian ini menggunakan metode pendekatankualitatif dengan studi literatur, wawancara,dan observasi. Pembuatan karya film dokumenter ini melalui beberapa tahapan hingga karya ini utuh yaitu: pra produksi, produksi, hingga pasca produksi hal pertama yang dilakukan pada tahap pra produksi adalah observasi, wawancara, membuat konsep karya yang terdiri dari: penentuan ide, film statment, premis, sinopsis, treatment, dan shooting list. Pada tahap produksi sebagai sutradara harus menjaga konsep ide cerita selama proses syutingberlangsung.Pada tahap pasca produksi melakukan editinghasil syuting pengambilan gambar berdasarkan treatmentdan outline scene.Hasil penelitian ini dapat memvisualkan seorang difabel pengajar bahasa Inggris daring dengan teori penyutradaraan pada film dokumenter, dengan menunjukan bahwa melalui film dokumenter dapat dijadikan sebagai media pembelajaran mengenai kemampuan lebih seorang difabel dan dapat menjadi sumber motivasi baik untuk penyandang difabel maupun manusia dengan keadaan normal pada umumnya.Kata Kunci: Difabel,pengajar bahasa Inggris daring, film dokumenter, ekspositori, sutradara

Numerical Implementation of GMNIA for Steel Frame with Nonsymmetric Sections

Steel members using nonsymmetric sections are often used for their structural efficiency in contemporary construction. However, traditional inelastic analysis methods are mostly derived from regular hot-rolled steel sections with symmetrical shapes; the adoption of those methods may lead to significant differences when analyzing members with nonsymmetric sections. In view of such a need, this research proposed an innovative method for the Geometrically and Materially Nonlinear Analysis with Imperfections (GMNIA) of steel frame with symmetric and nonsymmetric sections. An improved line-element formulation derived based on its principal axis is adopted. The tangent stiffness matrix of the element is formulated based on the nonsymmetric section assumption, explicitly modeling the noncoincidence of the shear center and centroid for considering the Wagner effects. The proposed line-element can capture complex buckling behaviors of the members with nonsymmetric sections. Then, the Concentrated plasticity (CP) model is integrated into the element tangent stiffness matrix to consider the material nonlinearity, where the full-yield criterion using the yield surface is adopted. As part of this, a rigorous cross-section analysis method has been developed to generate the yield surfaces of arbitrary steel sections regardless of shapes. Such yield surfaces will be used to evaluate the full-yield condition, and the gradients to the yield surfaces will be calculated and used to control the plastic flow. The development of this GMINA for steel frame with nonsymmetric sections is presented in detail, as well as the derivation of the mathematical formulations. At last, two groups of verification examples are provided to validate the accuracy of the yield surface generation method and the proposed GMINA.The work described in this paper was partially supported by grants from the Research Grants Council of the Hong Kong Special Administrative Regain, China (Project No. PolyU/21E/15203121), and a grant from the National Natural Science Foundation of China (No. 52008410)

Q-Band Millimeter-Wave Antennas: An Enabling Technology for MultiGigabit Wireless Backhaul

[EN] The bandwidth demands in mobile communication systems are growing exponentially day by day as the number of users has increased drastically over the last five years. This mobile data explosion, together with the fixed service limitations, requires a new approach to support this increase in bandwidth demand. Solutions based on lower-frequency microwave wireless systems may be able to meet the bandwidth demand in a short term. However, with the small-cell mass deployment requiring total capacities of 1 Gb/s/km2, scalable, multigigabit backhaul systems are required. Millimeter-wave technology fits nicely into these new backhaul scenarios as it provides extended bandwidth for high-capacity links and adaptive throughput rate, which allows efficient and flexible deployment. Besides these advantages, millimeter-wave solutions become even more attractive when the cost of backhaul solutions and the cost of spectrum licenses are factored in. Compared to the cost of laying fiber to a cell base station, which is the only other scalable solution, the millimeter-wave solution becomes the most appropriate approach.The research leading to these results received funding from the European Commission's seventh Framework Programme under grant agreement 288267.Vilar Mateo, R.; Czarny, R.; Lee, ML.; Loiseaux, B.; Sypek, M.; Makowski, M.; Martel, C.... (2014). Q-Band Millimeter-Wave Antennas: An Enabling Technology for MultiGigabit Wireless Backhaul. IEEE Microwave Magazine. 15(4):121-130. https://doi.org/10.1109/MMM.2014.2308769S12113015

Social Europe. No 2/87

BACKGROUND: DNA methylation is an important type of epigenetic modification involved in gene regulation. Although strong DNA methylation at promoters is widely recognized to be associated with transcriptional repression, many aspects of DNA methylation remain not fully understood, including the quantitative relationships between DNA methylation and expression levels, and the individual roles of promoter and gene body methylation. RESULTS: Here we present an integrated analysis of whole-genome bisulfite sequencing and RNA sequencing data from human samples and cell lines. We find that while promoter methylation inversely correlates with gene expression as generally observed, the repressive effect is clear only on genes with a very high DNA methylation level. By means of statistical modeling, we find that DNA methylation is indicative of the expression class of a gene in general, but gene body methylation is a better indicator than promoter methylation. These findings are general in that a model constructed from a sample or cell line could accurately fit the unseen data from another. We further find that promoter and gene body methylation have minimal redundancy, and either one is sufficient to signify low expression. Finally, we obtain increased modeling power by integrating histone modification data with the DNA methylation data, showing that neither type of information fully subsumes the other. CONCLUSION: Our results suggest that DNA methylation outside promoters also plays critical roles in gene regulation. Future studies on gene regulatory mechanisms and disease-associated differential methylation should pay more attention to DNA methylation at gene bodies and other non-promoter regions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13059-014-0408-0) contains supplementary material, which is available to authorized users

Eosinophil Morphology Eosinophil granules and degranulation

Endogenous DNA damage is causally associated with the functional decline and transformation of stem cells that characterize aging. DNA lesions that have escaped DNA repair can induce replication stress and genomic breaks that induce senescence and apoptosis. It is not clear how stem and proliferating cells cope with accumulating endogenous DNA lesions and how these ultimately affect the physiology of cells and tissues. Here we have addressed these questions by investigating the hematopoietic system of mice deficient for Rev1, a core factor in DNA translesion synthesis (TLS), the postreplicative bypass of damaged nucleotides. Rev1 hematopoietic stem and progenitor cells displayed compromised proliferation, and replication stress that could be rescued with an antioxidant. The additional disruption of Xpc, essential for global-genome nucleotide excision repair (ggNER) of helix-distorting nucleotide lesions, resulted in the perinatal loss of hematopoietic stem cells, progressive loss of bone marrow, and fatal aplastic anemia between 3 and 4 months of age. This was associated with replication stress, genomic breaks, DNA damage signaling, senescence, and apoptosis in bone marrow. Surprisingly, the collapse of the Rev1Xpc bone marrow was associated with progressive mitochondrial dysfunction and consequent exacerbation of oxidative stress. These data reveal that, to protect its genomic and functional integrity, the hematopoietic system critically depends on the combined activities of repair and replication of helix-distorting oxidative nucleotide lesions by ggNER and Rev1-dependent TLS, respectively. The error-prone nature of TLS may provide mechanistic understanding of the accumulation of mutations in the hematopoietic system upon aging

The Evolution of Gene Expression QTL in Saccharomyces cerevisiae

Understanding the evolutionary forces that influence patterns of gene expression variation will provide insights into the mechanisms of evolutionary change and the molecular basis of phenotypic diversity. To date, studies of gene expression evolution have primarily been made by analyzing how gene expression levels vary within and between species. However, the fundamental unit of heritable variation in transcript abundance is the underlying regulatory allele, and as a result it is necessary to understand gene expression evolution at the level of DNA sequence variation. Here we describe the evolutionary forces shaping patterns of genetic variation for 1206 cis-regulatory QTL identified in a cross between two divergent strains of Saccharomyces cerevisiae. We demonstrate that purifying selection against mildly deleterious alleles is the dominant force governing cis-regulatory evolution in S. cerevisiae and estimate the strength of selection. We also find that essential genes and genes with larger codon bias are subject to slightly stronger cis-regulatory constraint and that positive selection has played a role in the evolution of major trans-acting QTL

Brown-Vialetto-Van Laere and Fazio Londe syndrome is associated with a riboflavin transporter defect mimicking mild MADD: a new inborn error of metabolism with potential treatment

We report on three patients (two siblings and one unrelated) presenting in infancy with progressive muscle weakness and paralysis of the diaphragm. Metabolic studies revealed a profile of plasma acylcarnitines and urine organic acids suggestive of a mild form of the multiple acyl-CoA dehydrogenation defect (MADD, ethylmalonic/adipic acid syndrome). Subsequently, a profound flavin deficiency in spite of a normal dietary riboflavin intake was established in the plasma of all three children, suggesting a riboflavin transporter defect. Genetic analysis of these patients demonstrated mutations in the C20orf54 gene which encodes the human homolog of a rat riboflavin transporter. This gene was recently implicated in the Brown-Vialetto-Van Laere syndrome, a rare neurological disorder which may either present in infancy with neurological deterioration with hypotonia, respiratory insufficiency and early death, or later in life with deafness and progressive ponto-bulbar palsy. Supplementation of riboflavin rapidly improved the clinical symptoms as well as the biochemical abnormalities in our patients, demonstrating that high dose riboflavin is a potential treatment for the Brown-Vialetto-Van Laere syndrome as well as for the Fazio Londe syndrome which is considered to be the same disease entity without the deafnes

Thermotropic Liquid-Crystalline and Light-Emitting Properties of Bis(4-aalkoxyphenyl) Viologen Bis(triflimide) Salts

A series of bis(4-alkoxyphenyl) viologen bis(triflimide) salts with alkoxy chains of different lengths were synthesized by the metathesis reaction of respective bis(4-alkoxyphenyl) viologen dichloride salts, which were in turn prepared from the reaction of Zincke salt with the corresponding 4-n-alkoxyanilines, with lithium triflimide in methanol. Their chemical structures were characterized by 1H and 13C nuclear magnetic resonance spectra and elemental analysis. Their thermotropic liquid-crystalline (LC) properties were examined by differential scanning calorimetry, polarizing optical microscopy, and variable temperature X-ray diffraction. Salts with short length alkoxy chains had crystal-to-liquid transitions. Salts of intermediate length alkoxy chains showed both crystal-to-smectic A (SmA) transitions, Tms, and SmA-to-isotropic transitions, Tis. Those with longer length of alkoxy chains had relatively low Tms at which they formed the SmA phases that persisted up to the decomposition at high temperatures. As expected, all of them had excellent thermal stabilities in the temperature range of 330–370 °C. Their light-emitting properties in methanol were also included

The mosaic of KIR haplotypes in rhesus macaques

To further refine and improve biomedical research in rhesus macaques, it is necessary to increase our knowledge concerning both the degree of allelic variation (polymorphism) and diversity (gene copy number variation) in the killer cell immunoglobulin-like receptor (KIR) gene cluster. Pedigreed animals in particular should be studied, as segregation data will provide clues to the linkage of particular KIR genes/alleles segregating on a haplotype and to its gene content as well. A dual strategy allowed us to screen the presence and absence of genes and the corresponding transcripts, as well as to track differences in transcription levels. On the basis of this approach, 14 diverse KIR haplotypes have been described. These haplotypes consist of multiple inhibitory and activating Mamu-KIR genes, and any gene present on one haplotype may be absent on another. This suggests that the cost of accelerated evolution by recombination may be the loss of certain framework genes on a haplotype

Comparison of Strategies to Detect Epistasis from eQTL Data

Genome-wide association studies have been instrumental in identifying genetic variants associated with complex traits such as human disease or gene expression phenotypes. It has been proposed that extending existing analysis methods by considering interactions between pairs of loci may uncover additional genetic effects. However, the large number of possible two-marker tests presents significant computational and statistical challenges. Although several strategies to detect epistasis effects have been proposed and tested for specific phenotypes, so far there has been no systematic attempt to compare their performance using real data. We made use of thousands of gene expression traits from linkage and eQTL studies, to compare the performance of different strategies. We found that using information from marginal associations between markers and phenotypes to detect epistatic effects yielded a lower false discovery rate (FDR) than a strategy solely using biological annotation in yeast, whereas results from human data were inconclusive. For future studies whose aim is to discover epistatic effects, we recommend incorporating information about marginal associations between SNPs and phenotypes instead of relying solely on biological annotation. Improved methods to discover epistatic effects will result in a more complete understanding of complex genetic effects