What did we learn from 'omics' studies in osteoarthritis

Review article[Abstract] Purpose of review: 'Omics' technologies developed for the massive analysis of the major biologically relevant molecules (genes, proteins, metabolites) have been applied to the study of osteoarthritis (OA) for more than a decade. Recent findings: 'Omics' studies have undoubtedly contributed to increase the knowledge on pathogenic processes related with OA and have provided hundreds to thousands of molecules that might have a putative biomarker utility for this disease. Summary: This review describes the most recent 'omics' studies in OA research, their conclusions, and discuss those remaining challenges. Still many validation studies must be performed in large and well-characterized cohorts for the translation of the findings from 'omics' strategies to clinical applications. The development of tools for the intelligent integration of 'omics' data with clinical and imaging information is also mandatory to take full profit of the work that has been already performed.Instituto de Salud Carlos III; PI14/01707Instituto de Salud Carlos III; PI14/01254Instituto de Salud Carlos III; PI16/02124Instituto de Salud Carlos III; CIBERCB06/ 01/0040Instituto de Salud Carlos III; RETIC-RIER-RD12/0009/0018Instituto de Salud Carlos III; PT13/000

Análisis comparativo de células madre mesenquimales procedentes de pacientes artrósicos y sanos en diferenciación condrogénica

Comunicaciones a congreso

Gas migration in a Cenozoic clay: experimental results and numerical modelling

Gas migration through a potential host clay formation for the geological disposal of radioactive waste in Belgium is experimentally investigated in the laboratory, and numerical modelling is performed to help in the interpretation of the results. Selected air injection tests under oedometer conditions on initially saturated Boom Clay samples with oriented bedding planes are presented in the paper. Priority in the experimental programme was given to the study of the deformation response along the injection and dissipation stages, as well as to the analysis of the pore network changes, which detect the opening of fissures that can act as preferential air pathways. The experimental results were simulated using a fully coupled hydro-mechanical finite element code, which incorporates an embedded fracture permeability model to account for the simulation of the gas flow along preferential pathways. Clay intrinsic permeability and its retention curve were assumed to be dependent on strains through fracture aperture changes. The numerical results could reproduce upstream/downstream pressures, outflow volume and soil volume change accurately. The experimental results, combined with the numerical simulation, provide good insight into the role of the volumetric response and of the bedding planes on the air transport properties of Boom Clay samples, confirming that fracture aperture occurs during gas injection, which eventually dominates further injection and pressure release stages.Peer ReviewedPostprint (author's final draft

School and Family Environment is Positively Associated with Extracurricular Physical Activity Practice among 8 to 16 Years Old School Boys and Girls

Extracurricular physical activity in children and adolescents can help achieve compliance with the World Health Organization (WHO) recommendations for physical activity. The purpose of this study was to evaluate factors in school and family environments of children and adolescents in Spain that could be related to the practice of extracurricular physical activity. Multistage random cluster sampling was conducted to include 128 schools with the participation of 10,096 students between the ages of 7 and 16. Participants completed the survey of sports habits designed by the National Sports Council. The results revealed a higher participation in extracurricular sports activities among boys (OR: 1.67 (1.5–1.9)) and students in primary education (up to 12 years old) (OR: 1.8 (1.7–2.0)). Likewise, a statistically significant relationship (p < 0.005) was observed between families where another family member practiced sports and lower number of screen time hours, improved academic performance, and better self-perceived health. Participation of children and adolescents in extracurricular sports activities seems to be associated with their immediate environment. It is therefore essential to emphasize the importance of establishing physical activity habits from an early age in family and school environments

Búsqueda de biomarcadores séricos de artrosis mediante depleción secuencias combinada con DIGE

Comunicaciones a congreso

Reducing high abundance proteins in human serum: depletion versus equalization

Comunicaciones a congreso

Platelet-rich plasma in osteoarthritis treatment: review of current evidence

Review[Abstract] Platelet-rich plasma (PRP) is defined as a volume of plasma with a platelet concentration higher than the average in peripheral blood. Many basic, preclinical and even clinical case studies and trials report PRP’s ability to improve musculoskeletal conditions including osteoarthritis, but paradoxically, just as many conclude it has no effect. The purpose of this narrative review is to discuss the available relevant evidence that supports the clinical use of PRP in osteoarthritis, highlighting those variables we perceive as critical. Here, recent systematic reviews and meta-analyses were used to identify the latest randomized controlled trials (RCTs) testing a PRP product as an intra-articular treatment for knee osteoarthritis, compared with an intra-articular control (mostly hyaluronic acid). Conclusions in the identified RCTs are examined and compared. In total, five recent meta-analyses and systematic reviews were found meeting the above criteria. A total of 19 individual trials were identified in the five reviews but only 9 were level of evidence I RCTs, and many had moderate or high risks of bias. At present, results from these RCTs seem to favor PRP use over other intra-articular treatments to improve pain scales in the short and medium term (6–12 months), but the overall level of evidence is low. As a result, clinical effectiveness of PRP for knee osteoarthritis treatment is still under debate. This is, prominently, the result of a lack of standardization of PRP products, scarceness of high quality RCTs not showing high risks of bias, and poor patient stratification for inclusion in the RCTs.Insituto de Salud Carlos III PI12/00329Insituto de Salud Carlos III; PI16/0212

The chalcolithic “pit field” of Fuente Celada (Burgos): preliminary data and perspectives

Se presentan los resultados de la excavación de un yacimiento del tipo “campo de hoyos”, en su mayoría calcolíticos precampaniformes con una pequeña zona de ocupación anterior durante el Neolítico. Destaca la presencia de varias inhumaciones humanas en alguno de los hoyos y de restos asociados al hábitat (manteados de barro y elementos de molienda) en una zona, donde debió de estar situado un pequeño poblado de cabañas cuyos restos han desaparecido al estar estratigráficamente colocados en superficie. Todo ello apunta a una estructura de aprovechamiento económico de tipo estable, con inversión a largo plazo en los medios de producción, al contrario de las hipótesis que postulan una economía itinerante durante este período de la prehistoria de la submeseta norte españolaThe results are presented of the archaeological excavation of a ‘Pit Field’ site in the Northern Spanish Plateau. While a small part of the site was occupied during the Neolithic, most of the pits belong chronologically and culturally to the “Pre-Bell Beaker” Chalcolitith period. A few human graves were recovered inside the pits, together with indications of a settlement area (mud fragments used in the huts and stone mortars) whose remains have disappeared due to postdepositional disturbance. The evidence seems to support recent hypothesis claiming a sedentary exploitation of the milieu, with long-term economic investment on the nearby landscape, in opposition to traditional interpretations describing the prehistoric economy of the area as fundamentally mobile

Hypoxia conditions differentially modulate normal and osteoarthritic chondrocyte proteomes

Comunicaciones a congreso