Radio Spectral Index Analysis and Classes of Ejection in LS I +61 303

LS I +61303 is a gamma-ray binary with periodic radio outbursts coincident with the orbital period of P=26.5 d. The origin of the radio emission is unclear,it could be due either to a jet, as in microquasars, or to the shock boundary between the Be star and a possible pulsar wind. We here analyze the radio spectral index over 6.7 yr from Green Bank Interferometer data at 2.2 GHz and 8.3 GHz. We find two new characteristics in the radio emission. The first characteristic is that the periodic outbursts indeed consist of two consecutive outbursts; the first outburst is optically thick, whereas the second outburst is optically thin. The spectrum of LS I +61 303 is well reproduced by the shock-in-jet model commonly used in the context of microquasars and AGNs: the optically thin spectrum is due to shocks caused by relativistic plasma ("transient jet") traveling through a pre-existing much slower steady flow ("steady jet"). This steady flow is responsible for the preceding optically thick spectrum. The second characteristic we find is that the observed spectral evolution, from optically thick to optically thin emission, occurs twice during the orbital period. We observed this occurrence at the orbital phase of the main 26.5 d outburst and also at an earlier phase, shifted by $\Delta \Phi \sim$ 0.3 (i.e almost 8 days before). We show that this result qualitatively and quantitatively agrees with the two-peak accretion/ejection model proposed in the past for LS I +61303. We conclude that the radio emission in LS I +61303 originates from a jet and suggest that the variable TeV emission comes from the usual Compton losses expected as an important by-product in the shock-in-jet theory.Comment: 27 pages, 7 figures, accepted for publication in Ap

The dust-enshrouded microquasar candidate AX J1639.0-4642 = IGR J16393-4643

We present a multiwavelength study of the field containing the unidentified X-ray source AX J1639.0-4642, discovered with the ASCA observatory and recently detected with the IBIS telescope, onboard the INTEGRAL satellite, dubbed IGR J16393-4643. The huge hydrogen column density towards the source, the hard spectral index in the 0.7-10 keV band and its flux variability suggest that the source is a High Mass X-ray Binary (HMXB) enshrouded by dust. Our search reveals the presence of a non-thermal radio counterpart within the X-ray error box. After a study of the broadband emission from X-rays to the radio domain, we propose that AX J1639.0-4642 is a dust-enshrouded Microquasar (MQ) candidate. In addition, the X-ray source is well within the 95% location contour of the unidentified gamma-ray source 3EG J1639-4702. The main properties of AX J1639.0-4642/3EG J1639-4702 are consistent with those of two other MQs previously proposed to display high-energy gamma-ray emission.Comment: 7 pages, 5 figures. Accepted for publication in A&A. Title and discussion on the possible NIR counterpart have been modifie

Enteric fever: a slow response to an old plague

Man is irremediably embedded in nature with complex interactions with all living organisms. Historically, the establishment of contemporary human societies has been influenced by our coexistence with other microorganisms living in highly interconnected habitats and ecologies. As a result, with the progression from unicellular to multicellular life, bacteria have coexisted with humans. In this biological journey, while there are important benefits provided by bacterial guests to the human host living in complex relationships and becoming part of their microbiome, some organisms are able to cause a wide spectrum of diseases. Among the large Enterobacteriaceae family, the genus Salmonella, a pathotype of Escherichia coli, is one example. Salmonella is further classified into S. enterica and S. bongori serotypes based on its lipopolysaccharide cell wall (somatic O antigen), its flagellar (H antigen), and its surface Vi antigen (present only in S. typhi, S. Paratyphi C, Citrobacter freundii, and S. Dublin) [1]. S. enterica subspecies I, one of the six subspecies of S. enterica, is a major contributor to human disease (Fig 1) [2]. This group of pathogens includes those frequently causing gastroenteritis, such as S. Typhimurium, those causing invasive disease in the forms of bacteremia, such as S. Choleraesius, or the typhoidal Salmonella species causing enteric fever, including S. typhi (typhoid fever) and S. Paratyphi A, B, and C (paratyphoid fever) [1,2]

Characterization of Exosporium Layer Variability of Clostridioides difficile Spores in the Epidemically Relevant Strain R20291.

Indexación: Scopus.Clostridioides difficile is a Gram-positive anaerobic intestinal pathogenic bacterium and the causative agent of antibiotic-associated diarrhea. C. difficile spore is a dormant state which acts as a vehicle of transmission and infection. In C. difficile spores, the outermost exosporium layer is the first barrier of interaction with the host and should carry spore ligands involved in spore-host interactions. C. difficile forms two types of spores (i.e., thin and thick exosporium layers). In this communication, we contribute to understand several biological aspects of these two exosporium morphotypes. By transmission electron microscopy, we demonstrate that both exosporium morphotypes appear simultaneously during sporulation and that spore-coat laminations are formed under anaerobic conditions. Nycodenz density-gradient allows enrichment of spores with a thick-exosporium layer morphotype and presence of polar appendage. Using translational fluorescent fusions with exosporium proteins BclA3, CdeA, CdeC, and CdeM as well as with several spore coat proteins, we observed that expression intensity and distribution of SNAP-translational fusions in R20291 strain is highly heterogeneous. Electron micrographs demonstrate that multicopy expression of CdeC, but not CdeM, SNAP translational fusion, increases the abundance of the thick exosporium morphotype. Collectively, these results raise further questions on how these distinctive exosporium morphotypes are made during spore formation. © Copyright © 2020 Pizarro-Guajardo, Calderón-Romero, Romero-Rodríguez and Paredes-Sabja.https://www.frontiersin.org/articles/10.3389/fmicb.2020.01345/ful

Primary herpes simplex virus type 1 infection with acute liver failure in solid organ transplantation: Report of three cases and review

Herpes virus infections is not uncommon in solid organ transplantation patients. We report 3 cases with primary Herpes simplex virus type-1 (HSV1) infection with acute liver failure (ALF). This is a rare and potentially fatal entity that could be a donor-derived infection. Although the initial clinical presentation is non-specific, it should be considered as a differential diagnosis in HSV-negative serology patients with liver failure and empirical treatment must be started in combination with a drastic reduction of immunosuppression. A strategy of HSV prophylaxis for pre-transplant HSV seronegative patients must be stablished in order to reduce the risk of clinical disease.© 2022 Published by Elsevier Ltd

Image informatics strategies for deciphering neuronal network connectivity

Brain function relies on an intricate network of highly dynamic neuronal connections that rewires dramatically under the impulse of various external cues and pathological conditions. Among the neuronal structures that show morphologi- cal plasticity are neurites, synapses, dendritic spines and even nuclei. This structural remodelling is directly connected with functional changes such as intercellular com- munication and the associated calcium-bursting behaviour. In vitro cultured neu- ronal networks are valuable models for studying these morpho-functional changes. Owing to the automation and standardisation of both image acquisition and image analysis, it has become possible to extract statistically relevant readout from such networks. Here, we focus on the current state-of-the-art in image informatics that enables quantitative microscopic interrogation of neuronal networks. We describe the major correlates of neuronal connectivity and present workflows for analysing them. Finally, we provide an outlook on the challenges that remain to be addressed, and discuss how imaging algorithms can be extended beyond in vitro imaging studies

Active wetting of epithelial tissues

Development, regeneration and cancer involve drastic transitions in tissue morphology. In analogy with the behavior of inert fluids, some of these transitions have been interpreted as wetting transitions. The validity and scope of this analogy are unclear, however, because the active cellular forces that drive tissue wetting have been neither measured nor theoretically accounted for. Here we show that the transition between 2D epithelial monolayers and 3D spheroidal aggregates can be understood as an active wetting transition whose physics differs fundamentally from that of passive wetting phenomena. By combining an active polar fluid model with measurements of physical forces as a function of tissue size, contractility, cell-cell and cell-substrate adhesion, and substrate stiffness, we show that the wetting transition results from the competition between traction forces and contractile intercellular stresses. This competition defines a new intrinsic lengthscale that gives rise to a critical size for the wetting transition in tissues, a striking feature that has no counterpart in classical wetting. Finally, we show that active shape fluctuations are dynamically amplified during tissue dewetting. Overall, we conclude that tissue spreading constitutes a prominent example of active wetting --- a novel physical scenario that may explain morphological transitions during tissue morphogenesis and tumor progression