ALC1/eIF4A1-mediated regulation of CtIP mRNA stability controls DNA end resection

During repair of DNA double-strand breaks, resection of DNA ends influences how these lesions will be repaired. If resection is activated, the break will be channeled through homologous recombination; if not, it will be simply ligated using the non-homologous end-joining machinery. Regulation of resection relies greatly on modulating CtIP, which can be done by modifying: i) its interaction partners, ii) its post-translational modifications, or iii) its cellular levels, by regulating transcription, splicing and/or protein stability/degradation. Here, we have analyzed the role of ALC1, a chromatin remodeler previously described as an integral part of the DNA damage response, in resection. Strikingly, we found that ALC1 affects resection independently of chromatin remodeling activity or its ability to bind damaged chromatin. In fact, it cooperates with the RNA-helicase eIF4A1 to help stabilize the most abundant splicing form of CtIP mRNA. This function relies on the presence of a specific RNA sequence in the 5' UTR of CtIP. Therefore, we describe an additional layer of regulation of CtIP-at the level of mRNA stability through ALC1 and eIF4A1

IEOOS, the Spanish Institute of Oceanography integrated ocean Observing System

Since its foundation, 100 years ago, the Spanish Institute of Oceanography (IEO) has been observing and measuring the ocean characteristics. Some systems like the tide gauges network has been working for more than 60 years. The IEO standard sections began at different moments depending on the local projects, and nowadays there are XXX coastal stations and XXX deep ones that are systematically sampled, taking physical as well as biochemical measurements. At this moment, the IEO Observing System (IEOOS) includes 4 permanent moorings equipped with currentmeters, an open-sea ocean-meteorological buoy offshore Santander and an SST satellital image reception station. It also supports the Spanish contribution to the ARGO international program with 47 deployed profilers, and continuous monitoring thermosalinometers, meteorological stations and ADCP installed on the IEO research vessels. All these networks are linked to international iniciatives like SeaDataNet, Emodnet, IbiROOS and MONGOOS. The system is completed with the IEO contribution to the RAIA and Gibraltar observatories, and the development of regional prediction models. All these systematic measurements allow IEO to give responses to ocean research activities, official agencies requirements and industrial and main society demands

Crítica de Libros.

V. APARICIO BASAURI (coord.), Evaluación de servicios de salud mental, Madrid, A.E.N., 1993. Ramón Esteban Arnáiz. Josefina MASS HESSE y Amelia TESORO ARRARTE (coord.), Mujer y salud mental. Mitos y realidades, Madrid, A.E.N., 1993. M. López Peña G. LANTERI-LAURA, Les hallucinations, París, Masson, 1991. José María Álvarez Zvi LOTHANE, In defense of Schreber, Nueva Jersey, The Analytic Press, 1991. Martín Lorenzo Vargas Aragón y Jesús María Rodríguez Huidobro. Carmen SÁEZ BUENAVENTURA, ¿La liberación era esto? Mujeres, vidas y crisis, Madrid, Temas de Hoy, 1993. Velia Chavarría Daniela APARICIO Y otros, Salud Mental, Barcelona, Doyma, 1992. José Leal Rubio Jorge ALEMÁN, Cuestiones antifilosóficas de Jacques Lacan, Buenos Aires, Atuel, 1993. Fernando Colina Gilles DELEUZE, Critique et clinique, París, Minuit, 1993. Mauricio Jaló

Crítica de Libros.

V. APARICIO BASAURI (coord.), Evaluación de servicios de salud mental, Madrid, A.E.N., 1993. Ramón Esteban Arnáiz. Josefina MASS HESSE y Amelia TESORO ARRARTE (coord.), Mujer y salud mental. Mitos y realidades, Madrid, A.E.N., 1993. M. López Peña G. LANTERI-LAURA, Les hallucinations, París, Masson, 1991. José María Álvarez Zvi LOTHANE, In defense of Schreber, Nueva Jersey, The Analytic Press, 1991. Martín Lorenzo Vargas Aragón y Jesús María Rodríguez Huidobro. Carmen SÁEZ BUENAVENTURA, ¿La liberación era esto? Mujeres, vidas y crisis, Madrid, Temas de Hoy, 1993. Velia Chavarría Daniela APARICIO Y otros, Salud Mental, Barcelona, Doyma, 1992. José Leal Rubio Jorge ALEMÁN, Cuestiones antifilosóficas de Jacques Lacan, Buenos Aires, Atuel, 1993. Fernando Colina Gilles DELEUZE, Critique et clinique, París, Minuit, 1993. Mauricio Jaló

IEOOS: the Spanish Institute of Oceanography Observing System

En prens

Marco activo de recursos de innovación docente: Madrid

Una guía de espacios e instituciones para actividades educativas complementarias en enseñanza secundaria y Formación Profesional

Towards precision medicine: defining and characterizing adipose tissue dysfunction to identify early immunometabolic risk in symptom-free adults from the GEMM family study

Interactions between macrophages and adipocytes are early molecular factors influencing adipose tissue (AT) dysfunction, resulting in high leptin, low adiponectin circulating levels and low-grade metaflammation, leading to insulin resistance (IR) with increased cardiovascular risk. We report the characterization of AT dysfunction through measurements of the adiponectin/leptin ratio (ALR), the adipo-insulin resistance index (Adipo-IRi), fasting/postprandial (F/P) immunometabolic phenotyping and direct F/P differential gene expression in AT biopsies obtained from symptom-free adults from the GEMM family study. AT dysfunction was evaluated through associations of the ALR with F/P insulin-glucose axis, lipid-lipoprotein metabolism, and inflammatory markers. A relevant pattern of negative associations between decreased ALR and markers of systemic low-grade metaflammation, HOMA, and postprandial cardiovascular risk hyperinsulinemic, triglyceride and GLP-1 curves was found. We also analysed their plasma non-coding microRNAs and shotgun lipidomics profiles finding trends that may reflect a pattern of adipose tissue dysfunction in the fed and fasted state. Direct gene differential expression data showed initial patterns of AT molecular signatures of key immunometabolic genes involved in AT expansion, angiogenic remodelling and immune cell migration. These data reinforce the central, early role of AT dysfunction at the molecular and systemic level in the pathogenesis of IR and immunometabolic disorders

Multiancestry analysis of the HLA locus in Alzheimer’s and Parkinson’s diseases uncovers a shared adaptive immune response mediated by HLA-DRB1*04 subtypes

Across multiancestry groups, we analyzed Human Leukocyte Antigen (HLA) associations in over 176,000 individuals with Parkinson’s disease (PD) and Alzheimer’s disease (AD) versus controls. We demonstrate that the two diseases share the same protective association at the HLA locus. HLA-specific fine-mapping showed that hierarchical protective effects of HLA-DRB1*04 subtypes best accounted for the association, strongest with HLA-DRB1*04:04 and HLA-DRB1*04:07, and intermediary with HLA-DRB1*04:01 and HLA-DRB1*04:03. The same signal was associated with decreased neurofibrillary tangles in postmortem brains and was associated with reduced tau levels in cerebrospinal fluid and to a lower extent with increased Aβ42. Protective HLA-DRB1*04 subtypes strongly bound the aggregation-prone tau PHF6 sequence, however only when acetylated at a lysine (K311), a common posttranslational modification central to tau aggregation. An HLA-DRB1*04-mediated adaptive immune response decreases PD and AD risks, potentially by acting against tau, offering the possibility of therapeutic avenues

Efecto de diferentes combinaciones de temperatura y salinidad sobre el consumo específico de oxígeno en el camarón blanco Litopenaeus vannamei

The combined effect of different temperatures (20, 25 and 30°C) and salinities (15, 25, 35 and 45 psu) on the specific oxygen consumption (CEO) in Litopenaeus vannamei was determined. For this, CEO of 248 organisms of different body sizes (0.31 to 15.85 g) was measured. The CEO decreased when shrimp weight increased in all treatments without presenting a definite pattern when the salinity increased; even though all the combinations of temperature and salinity did not statistically show slopes significantly different in the regression models for CEO and organism weight relationships. The slope values suggest that at 20°C there is high energy expenditure at 15 psu, and at 25°C the highest energy consumption occurs at 45 psu. Whereas at 30°C, the lowest energy consumption occurred combined with the lowest salinity (15 psu). This may indicate that the isosmotic condition (point of lowest energy expenditure) tends to change with temperature, which may imply that the shrimp can be cultivated at high temperature (30°C) and at low salinities (15 psu) without increasing energy expenditure.Se analizó el efecto combinado de diferentes temperaturas (20, 25 y 30°C) y salinidades (15, 25, 35 y 45 ups) sobre el consumo específico de oxígeno (CEO) en Litopenaeus vannamei. Se midió el CEO en 248 organismos de distintas tamaños corporales (0,31 a 15,85 g). El CEO disminuyó con el aumento de peso corporal de los organismos en todos los tratamientos, sin observarse un patrón general definido de aumento del CEO conforme se incrementó la salinidad. Aunque no hubo diferencias estadísticas significativas en todas la combinaciones de temperatura-salinidad entre las pendientes de regresión que relacionan el CEO con el peso de los organismos, los valores de pendientes en particular sugieren que en 20°C se da un mayor gasto energético en la menor salinidad (15 ups) y en 25°C en la salinidad más alta (45 ups). Mientras que en 30°C, el menor gasto energético se presentó en la menor salinidad (15 ups). Lo cual puede indicar que la condición isosmótica (punto de menor gasto energético) tiende a modificarse con la temperatura, favoreciendo con ello que los camarones se puedan cultivar en alta temperatura (30°C) y baja salinidad (15 ups) sin que se presente un mayor gasto energético

El "Clima": una herramienta para la gestión de la información climatológica en Andalucía

Existe una versión de este artículo (solo disponible en la versión caché de Google) donde varían los coautoresEl Subsistema de Información de Climatología Ambiental (CLIMA) está formado por un conjunto de redes de observación meteorológica relacionadas mediante una aplicación informática que permite su uso conjunto por diversos organismos. Los datos, procedentes de estaciones manuales y automáticas, son convertidos a un formato único y, tras superar una serie de métodos de validación y de interpolación de lagunas pasan a formar parte de un banco de datos climático totalmente homogéneo. Con este Subsistema, una de las herramientas fundamentales dentro de la Estrategia Autonómica ante el Cambio Climático, se pretende dar respuesta a la creciente necesidad de datos climáticos de calidad, que permitan afrontar estudios sobre la evolución del clima en Andalucía y prever las posibles consecuencias de fenómenos como el cambio climático. Este subsistema de información se integra en la Red de Información Ambiental de Andalucía