Adelante / Endavant

Séptimo desafío por la erradicación de la violencia contra las mujeres del Institut Universitari d’Estudis Feministes i de Gènere "Purificación Escribano" de la Universitat Jaume

Cux1 Enables Interhemispheric Connections of Layer II/III Neurons by Regulating Kv1-Dependent Firing

Neuronal subtype-specific transcription factors (TFs) instruct key features of neuronal function and connectivity. Activity-dependent mechanisms also contribute to wiring and circuit assembly, but whether and how they relate to TF-directed neuronal differentiation is poorly investigated. Here we demonstrate that the TF Cux1 controls the formation of the layer II/III corpus callosum (CC) projections through the developmental transcriptional regulation of Kv1 voltage-dependent potassium channels and the resulting postnatal switch to a Kv1-dependent firing mode. Loss of Cux1 function led to a decrease in the expression of Kv1 transcripts, aberrant firing responses, and selective loss of CC contralateral innervation. Firing and innervation were rescued by re-expression of Kv1 or postnatal reactivation of Cux1. Knocking down Kv1 mimicked Cux1-mediated CC axonal loss. These findings reveal that activity-dependent processes are central bona fide components of neuronal TF-differentiation programs and establish the importance of intrinsic firing modes in circuit assembly within the neocortex.We are grateful to A. Fernández, R. Gutiérrez, A. Morales, and members of J.A.E. lab for excellent technical assistance; J.D. Macklis for invaluable technical help and discussion; C.O. Sorzano for statistical advice; C. Mark for editorial assistance; and G. Perea and P. Bovolenta for critical reading. F.M.R.-T. was supported by a fellowship from La Caixa Foundation. C.G.B. and L.A.W. are funded by the Spanish Ministerio de Ciencia e Innovación (MICINN), FPI-BES-2012-056011 to C.G.B. and JCI-2012-14147 to L.A.W.; S.A. holds a J.A.E.-Doc contract (JAEDOC014, 2010 call) from the CSIC program “Junta para la Ampliación de Estudios” cofunded by the European Social Fund. This work was funded by grant PHYSDEV (FIS2012-32349) to S.A., a grant from BBVA Foundation to M. Navarrete, grants CSD2010-00045 and SAF2011-24730 (from MICINN) to J.A.E., and a grant from Ramón Areces Foundation and grants SAF2011-23735 and BFU2014-55738-REDT (from MICINN) to M.Nieto

Digital reference: concept, technologies, and implementation in information centres

[ES]La referencia digital es el servicio que bibliotecas y centros de información ofrecen a sus usuarios para que realicen consultas a través de internet. Se describe el concepto de referencia digital y se exponen los diferentes tipos existentes a partir de diversos criterios. Se presentan las tecnologías que se emplean en la actualidad para la recepción de consultas y el envío de las respuestas. Se ofrecen las pautas para la puesta en marcha de un servicio de referencia digital partiendo de cuatro criterios: los agentes implicados, las técnicas empleadas, los recursos disponibles y la gestión del servicio. Se concluye destacando el valor de la referencia digital como un servicio que se ofrece en red y que amplía el espectro de usuarios.[EN]Digital reference is the service offered by libraries and information centres so that users can ask questions through the Internet. The concept of digital reference is described, along with the different typologies based on various criteria. The technologies used today for receiving questions and sending answers are presented. Guidelines are suggested for implementing a digital reference service, based on four criteria: the actors involved, the techniques used, the available resources and the management of the service. It concludes by highlighting the value of digital reference as an online service that expands the range of patrons