The silicon stable isotope distribution along the GEOVIDE section (GEOTRACES GA-01) of the North Atlantic Ocean

The stable isotope composition of dissolved silicon in seawater (δ30SiDSi) was examined at 10 stations along the GEOVIDE section (GEOTRACES GA-01), spanning the North Atlantic Ocean (40–60∘ N) and Labrador Sea. Variations in δ30SiDSi below 500 m were closely tied to the distribution of water masses. Higher δ30SiDSi values are associated with intermediate and deep water masses of northern Atlantic or Arctic Ocean origin, whilst lower δ30SiDSi values are associated with DSi-rich waters sourced ultimately from the Southern Ocean. Correspondingly, the lowest δ30SiDSi values were observed in the deep and abyssal eastern North Atlantic, where dense southern-sourced waters dominate. The extent to which the spreading of water masses influences the δ30SiDSi distribution is marked clearly by Labrador Sea Water (LSW), whose high δ30SiDSi signature is visible not only within its region of formation within the Labrador and Irminger seas, but also throughout the mid-depth western and eastern North Atlantic Ocean. Both δ30SiDSi and hydrographic parameters document the circulation of LSW into the eastern North Atlantic, where it overlies southern-sourced Lower Deep Water. The GEOVIDE δ30SiDSi distribution thus provides a clear view of the direct interaction between subpolar/polar water masses of northern and southern origin, and allow examination of the extent to which these far-field signals influence the local δ30SiDSi distribution

Alimentos funcionais : uma área estratégica para a BIOTEMPO

A BIOTEMPO – Consultoria em Biotecnologia, Lda. é uma empresa de base tecnológica que surgiu como resultado de um spin-off da Universidade do Minho. A sua actividade centra-se na prestação de serviços de consultoria e promoção de actividades de investigação e desenvolvimento. Actualmente, a BIOTEMPO participa em vários projectos de I&D, alguns dos quais são executados em parceria com empresas e universidades nacionais. No âmbito da sua Unidade de Biotecnologia Alimentar e Farmacêutica a BIOTEMPO tem-se dedicado ao desenvolvimento de novas tecnologias para a produção de ingredientes para alimentos funcionais, tendo elegido esta área como estratégica para o desenvolvimento da empresa

Design of a biosynthetic pathway for curcumin production in Escherichia coli

Curcumin is the yellow pigment from turmeric, a well known culinary spice produced from the herb Curcuma longa. Research over the last years has shown that curcumin presents a wide range of pharmacological effects, including anti-inflammatory, anti-oxidant and anticarcinogenic activity. Given its potential application in cancer treatment, there is an interest for industrial production of this natural compound. This work consists on a synthetic biology approach for the design of a heterologous pathway for curcumin synthesis in Escherichia coli, a widely used microbe in industrial biotechnology. Using pathway databases and literature research we have selected the best gene candidates for heterologous expression of a curcumin synthesis pathway in E. coli. The DNA sequences for these genes were retrieved from public databases and can be readily synthesized for insertion into the host using molecular biology techniques. The inclusion of this pathway in a recent genome-scale reconstruction of the metabolism of E. coli has enabled the in silico analysis of the production capabilities for this host. We have analysed the theoretical production yields and biomass growth under different experimental conditions. Using this model we have also searched for potential gene knockouts that partially redirect the metabolic flux to the heterologous pathway without compromising cellular growth. In overall, the methods used in this work allow the selection of the most suitable combination of experimental conditions and genetic manipulations for the design of an efficient biosynthetic pathway for curcumin production in E.coli

Looking at b-learning and online experience in higher education during COVID-19

COVID-19 caused changes in teaching practices at various levels of education, namely in higher education. This study aims to present an analysis of an experience with higher education students in a b-learning pedagogical model, before and during the current pandemic and in different moments of the confinement measures. The curricular unit analyzed started with a blended-learning approach since 2018, considering the importance of innovative pedagogical models in student learning and became an essential resource during the pandemic period. This case study was composed of a sample of 269 students from a Portuguese public University who attended the curricular unit Methods and Study Techniques, as optional, in their 1st year of degree, between February 2018 and March 2021.The data were processed using SPSS v.27, seeking to analyze different moments, according to the different confinement measures decreed by the Portuguese Government. Participants are aged between 17 and 64 years old, 62.5% female and 24.5% worker-students. It was found that regardless of the context of confinement, students showed positive satisfaction with the online course, with its contents, with the platform and with the b-learning pedagogical method, especially during total confinement when classes were in distance learning. The most valued aspects in this modality were the flexibility of teaching the curricular unit and time management. The acquired tools were highly valued by the students, first for the adaptability that gave them, but mainly to face the distance learning challenge in their 1st year of degree, giving them planning and organizing skills for autonomous work. Most students did not point out a disadvantage in this modality, although they expressed the importance of face-to-face classes that were suspended in the context of a pandemic.info:eu-repo/semantics/acceptedVersio

Ingredientes para alimentos funcionais: uma área de futuro?

Os prebióticos são ingredientes alimentares não digeríveis, geralmente oligossacarídeos, que exercem um efeito benéfico no indivíduo estimulando selectivamente o crescimento e/ou actividade de espécies bacterianas existentes no cólon, melhorando a saúde do hospedeiro. O mercado mundial deste tipo de produtos tem vindo a crescer a taxas bastante elevadas, apesar de os seus preços de venda serem ainda bastante elevados. Neste sentido, é de acreditar que o desenvolvimento de novos processos de produção biotecnológicos com menores custos associados impulsione ainda mais o mercado. O projecto “BIOLIFE – Ingredientes para alimentos funcionais” visa o desenvolvimento de processos alternativos de produção de prebióticos