The cytoplasmic domain of CD4 promotes the development of CD4 lineage T cells.

Thymocytes must bind major histocompatibility complex (MHC) proteins on thymic epithelial cells in order to mature into either CD8+ cytotoxic T cells or CD4+ helper T cells. Thymic precursors express both CD8 and CD4, and it has been suggested that the intracellular signals generated by CD8 or CD4 binding to class I or II MHC, respectively, might influence the fate of uncommitted cells. Here we test the notion that intracellular signaling by CD4 directs the development of thymocytes to a CD4 lineage. A hybrid protein consisting of the CD8 extracellular and transmembrane domains and the cytoplasmic domain of CD4 (CD884) should bind class I MHC but deliver a CD4 intracellular signal. We find that expression of a hybrid CD884 protein in thymocytes of transgenic mice leads to the development of large numbers of class I MHC-specific, CD4 lineage T cells. We discuss these results in terms of current models for CD4 and CD8 lineage commitment

Modified Bell-Plesset Effect with Compressibility: Application to Double-Shell Ignition Target Designs

The effect of spherical convergence on the fluid stability of collapsing and expanding bubbles was originally treated by Bell [Los Alamos Scientific Laboratory Report No. LA-1321 (1951)] and Plesset [J. Appl. Phys. 25, 96 (1954)]. The additional effect of fluid compressibility was also considered by Bell but was limited to the case of nonzero density on only one side of a fluid interface. A more general extension is developed which considers distinct time-dependent uniform densities on both sides of an interface in a spherically converging geometry. A modified form of the velocity potential is used that avoids an unphysical divergence at the origin [Goncharov et al., Phys. Plasmas 7, 5118 (2000); Lin et al., Phys. Fluids 14, 2925 (2002)]. Two consequences of this approach are that an instability proposed by Plesset for an expanding bubble in the limit of large interior density is now absent and application to inertial confinement fusion studies of stability becomes feasible. The model is applied to a proposed ignition double-shell target design [Amendt et al., Phys. Plasmas 9, 2221 (2002)] for the National Ignition Facility [Paisner et al., Laser Focus World 30, 75 (1994)] for studying the stability of the inner surface of an imploding high-Z inner shell. Application of the Haan [Phys. Rev. A 39, 5812 (1989)] saturation criterion suggests that ignition is possible

Dual, orthogonal, backlit pinhole radiography in OMEGA experiments

Backlit pinhole radiography used with ungated film as a detector creates x-ray radiographs with increased resolution and contrast. Current hydrodynamics experiments on the OMEGA Laser use a three-dimensional sinusoidal pattern as a seed perturbation for the study of instabilities. The structure of this perturbation makes it highly desirable to obtain two simultaneous orthogonal backlighting views. We accomplished this using two backlit pinholes each mounted 12 mm12mm from the target. The pinholes, of varying size and shape, were centered on 5 mm5mm square foils of 50 μm50μm thick Ta. The backlighting is by KK-alpha emission from a 500 μm500μm square Ti or Sc foil mounted 500 μm500μm from the Ta on a plastic substrate. Four laser beams overfill the metal foil, so that the expanding plastic provides radial tamping of the expanding metal plasma. The resulting x-rays pass through the target onto (ungated) direct exposure film (DEF). Interference between the two views is reduced by using a nose cone in front of the DEF, typically with a 9 mm9mm Ta aperture and with magnets to deflect electrons. Comparison of varying types of pinholes and film exposures will be presented from recent experiments as well as an analysis of the background noise created using this experimental technique.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87894/2/10E327_1.pd

On Validating an Astrophysical Simulation Code

We present a case study of validating an astrophysical simulation code. Our study focuses on validating FLASH, a parallel, adaptive-mesh hydrodynamics code for studying the compressible, reactive flows found in many astrophysical environments. We describe the astrophysics problems of interest and the challenges associated with simulating these problems. We describe methodology and discuss solutions to difficulties encountered in verification and validation. We describe verification tests regularly administered to the code, present the results of new verification tests, and outline a method for testing general equations of state. We present the results of two validation tests in which we compared simulations to experimental data. The first is of a laser-driven shock propagating through a multi-layer target, a configuration subject to both Rayleigh-Taylor and Richtmyer-Meshkov instabilities. The second test is a classic Rayleigh-Taylor instability, where a heavy fluid is supported against the force of gravity by a light fluid. Our simulations of the multi-layer target experiments showed good agreement with the experimental results, but our simulations of the Rayleigh-Taylor instability did not agree well with the experimental results. We discuss our findings and present results of additional simulations undertaken to further investigate the Rayleigh-Taylor instability.Comment: 76 pages, 26 figures (3 color), Accepted for publication in the ApJ

The molybdenum isotopic composition of the modern ocean

Natural variations in the isotopic composition of molybdenum (Mo) are showing increasing potential as a tool in geochemistry. Although the ocean is an important reservoir of Mo, data on the isotopic composition of Mo in seawater are scarce. We have recently developed a new method for the precise determination of Mo isotope ratios on the basis of preconcentration using a chelating resin and measurement by multiple-collector inductively coupled plasma mass spectrometry (MC-ICP-MS), which allows us to measure every stable Mo isotope. In this study, 172 seawater samples obtained from 9 stations in the Pacific, Atlantic, and Southern Oceans were analyzed, giving global coverage and the first full depth-profiles. The average isotope composition in δA/95Mo (relative to a Johnson Matthey Mo standard solution) was as follows: δ92/95Mo = –2.54 ± 0.16‰ (2SD), δ94/95Mo = –0.73 ± 0.19‰, δ96/95Mo = 0.85 ± 0.07‰, δ97/95Mo = 1.68 ± 0.08‰, δ98/95Mo = 2.48 ± 0.10‰, and δ100/95Mo = 4.07 ± 0.18‰. The δ values showed an excellent linear correlation with atomic mass of AMo (R2 = 0.999). Three-isotope plots for the Mo isotopes were fitted with straight lines whose slopes agreed with theoretical values for mass-dependent isotope fractionation. These results demonstrate that Mo isotopes are both uniformly distributed and follow a mass-dependent fractionation law in the modern oxic ocean. A common Mo standard is urgently required for the precise comparison of Mo isotopic compositions measured in different laboratories. On the other hand, our results strongly support the possibility of seawater as an international reference material for Mo isotopic composition

Transition to turbulence and effect of initial conditions on three-dimensional compressible mixing in planar blast-wave-driven systems

Perturbations on an interface driven by a strong blast wave grow in time due to a combination of Rayleigh–Taylor, Richtmyer–Meshkov, and decompression effects. In this paper, results from three-dimensional (3D) numerical simulations of such a system under drive conditions to be attainable on the National Ignition Facility [E. M. Campbell, Laser Part. Beams 9, 209 (1991)] are presented. Using the multiphysics, adaptive mesh refinement, higher order Godunov Eulerian hydrocode, Raptor [L. H. Howell and J. A. Greenough, J. Comput. Phys. 184, 53 (2003)], the late nonlinear instability evolution, including transition to turbulence, is considered for various multimode perturbation spectra. The 3D post-transition state differs from the 2D result, but the process of transition proceeds similarly in both 2D and 3D. The turbulent mixing transition results in a reduction in the growth rate of the mixing layer relative to its pretransition value and, in the case of the bubble front, relative to the 2D result. The post-transition spike front velocity is approximately the same in 2D and 3D. Implications for hydrodynamic mixing in core-collapse supernovae are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87764/2/056317_1.pd

A study on the performance of reproducible computations

Parallel computations are intrinsically non-reproducible, due to a combined effect of non-deterministic parallel reductions and nonassociative floating point operations. Different strategies have been proposed in literature to alleviate this issue or eliminate it altogether, however at present there is no study on the performance impact of associative floating point operations on large scale applications. In this work, we implement associative operations using binned doubles in MiniFE, and perform various performance tests on Cirrus and Fulhame, two state-ofthe-art HPC systems

TWO-DIMENSIONAL BLAST-WAVE-DRIVEN RAYLEIGH-TAYLOR INSTABILITY: EXPERIMENT AND SIMULATION

This paper shows results from experiments diagnosing the development of the Rayleigh–Taylor instability with two-dimensional initial conditions at an embedded, decelerating interface. Experiments are performed at the Omega Laser and use ~5 kJ of energy to create a planar blast wave in a dense, plastic layer that is followed by a lower density foam layer. The single-mode interface has a wavelength of 50 μm and amplitude of 2.5 μm. Some targets are supplemented with additional modes. The interface is shocked then decelerated by the foam layer. This initially produces the Richtmyer–Meshkov instability followed and then dominated by Rayleigh–Taylor growth that quickly evolves into the nonlinear regime. The experimental conditions are scaled to be hydrodynamically similar to SN1987A in order to study the instabilities that are believed to occur at the He/H interface during the blast-wave-driven explosion phase of the star. Simulations of the experiment were performed using the FLASH hydrodynamics code.United States. Dept. of Energy (Stewardship Science Academic Alliances Program. Grant DE FG03-99DP00284)United States. Dept. of Energy (Stewardship Science Academic Alliances Program. Grant DE-FG03-00SF22021