Macro and micronutrient accumulation in watermelon

The expansion of crop productivity such as watermelon can be reached by providing adequate nutrition in quantity and when plants need it most. It is known that well-nourished plants better resist biotic and abiotic stresses, being determinants for more sustainable management. In this sense, the objective of the present study was to evaluate the plant growth, the rate of nutrient absorption, the proportion of absorption, and estimate the quantity of exported nutrients by watermelon fruits. The experiment was implemented in randomized block design with eight treatments (phenological phases) - 33 and 40 (growth), 47 and 54 (flowering), 61 and 68 (filling), 75 and 82 (maturation) days after planting (DAP). Each evaluation consisted of plant collection for dry mass, macro, and micronutrient analysis in the shoot (leaves and stems), fruits, and the entire plant. The period between 61 and 68 DAP was the one with the highest plant dry matter increment. The accumulation of macronutrients in the plant shoot showed the following decreasing order: Ca>N>K>Mg>P>S, in the fruits: K>N>P>Ca>Mg>S and in the whole plant: K>N>Ca>P>Mg>S. For micronutrients, the decreasing order of accumulation for the shoot and the entire plant were Fe>Mn>Zn>B>Cu, and for fruits were Fe>B>Zn>Mn>Cu.The expansion of crop productivity such as watermelon can be reached by providing adequate nutrition in quantity and when plants need it most. It is known that well-nourished plants better resist biotic and abiotic stresses, being determinants for more sustainable management. In this sense, the objective of the present study was to evaluate the plant growth, the rate of nutrient absorption, the proportion of absorption, and estimate the quantity of exported nutrients by watermelon fruits. The experiment was implemented in randomized block design with eight treatments (phenological phases) - 33 and 40 (growth), 47 and 54 (flowering), 61 and 68 (filling), 75 and 82 (maturation) days after planting (DAP). Each evaluation consisted of plant collection for dry mass, macro, and micronutrient analysis in the shoot (leaves and stems), fruits, and the entire plant. The period between 61 and 68 DAP was the one with the highest plant dry matter increment. The accumulation of macronutrients in the plant shoot showed the following decreasing order: Ca>N>K>Mg>P>S, in the fruits: K>N>P>Ca>Mg>S and in the whole plant: K>N>Ca>P>Mg>S. For micronutrients, the decreasing order of accumulation for the shoot and the entire plant were Fe>Mn>Zn>B>Cu, and for fruits were Fe>B>Zn>Mn>Cu.The expansion of crop productivity such as watermelon can be reached by providing adequate nutrition in quantity and when plants need it most. It is known that well-nourished plants better resist biotic and abiotic stresses, being determinants for more sustainable management. In this sense, the objective of the present study was to evaluate the plant growth, the rate of nutrient absorption, the proportion of absorption, and estimate the quantity of exported nutrients by watermelon fruits. The experiment was implemented in randomized block design with eight treatments (phenological phases) - 33 and 40 (growth), 47 and 54 (flowering), 61 and 68 (filling), 75 and 82 (maturation) days after planting (DAP). Each evaluation consisted of plant collection for dry mass, macro, and micronutrient analysis in the shoot (leaves and stems), fruits, and the entire plant. The period between 61 and 68 DAP was the one with the highest plant dry matter increment. The accumulation of macronutrients in the plant shoot showed the following decreasing order: Ca>N>K>Mg>P>S, in the fruits: K>N>P>Ca>Mg>S and in the whole plant: K>N>Ca>P>Mg>S. For micronutrients, the decreasing order of accumulation for the shoot and the entire plant were Fe>Mn>Zn>B>Cu, and for fruits were Fe>B>Zn>Mn>Cu

Perceptions of occupational therapy students about experience of teaching-service-community integration

The present article tries understand the perceptions of students of the professional internship in occupational therapy on the experience of teaching-service-community integration experienced in a laboratory of daily life activity. It was a qualitative and exploratory study, conducted through semi-structured interviews. Content analysis was used with the thematic category: teaching-service-community integration. The sample consisted of 14 students who were enrolled in the course. The similarities, distinctions between the contents and the central ideas of the narratives about the thematic category were observed. The speeches generated the units of analysis: expectation of integrated practices, reality of teamwork, organizational process, exchange of experiences and suggestions. The results obtained pointed to the distance between students’ expectations and reality. The perceptions of the interviewees revealed that the work dynamics occurs without collective planning of the care actions and without interlocution with the teaching. The students suggested strategies to improve the teaching-service-community integration and to promote the formative process

Nursing care to woman in immediate puerperium: a narrative description

Objective: To describe the nursing care provided in the immediate postpartum though perceptions of nurses in a public hospital. Method: descriptive study with a qualitative approach. Study participants were nurses who attend the institution postpartum. Qualitative data were analyzed based on thematic content analysis. Results: from the analysis of discourses, categories encompassing the dimensions of the process of nursing work emerged: Managing; Assisting/intervening; Investigating/researching; and the teaching/learning in the nursing actions. Managing nursing actions underlies up in classical management theories; assistance is marked by biologism; there is lack of research in the work process; educational practices are based on the traditional health model and there is a shortage of actions on Continuing Education. Conclusion: the nursing practice must be based on articulated processes to generate practices that make up the entirety of health actions

Isolation, expansion, and characterization of equine adipose-derived stem cells

Devido aos excelentes resultados obtidos em pesquisas com células-tronco e suas aplicabilidades promissoras, a terapia com estas células encontra-se em momento de máxima ascensão, estimulando assim, inúmeras pesquisas. A fim de padronizar o cultivo e avaliar a viabilidade de células-tronco mesenquimais do tecido adiposo de equinos visando a terapia celular nesta espécie, conduziu-se um estudo de isolamento, cultivo e caracterização de tais células. Para tal, foi realizada coleta de tecido adiposo de cinco equinos sem raça definida, com aproximadamente cinco anos. Em seguida, o tecido foi refrigerado e transportado ao laboratório, onde foi realizado o protocolo de cultivo celular durante aproximadamente 14 dias. Após o término deste procedimento, uma alíquota de células foi encaminhada para a realização do teste de quantificação e viabilidade celular com Azul de Tripan 1%, sendo outra porção destinada à confirmação da linhagem de célula tronco mesenquimal por meio de imunofenotipagem com os anticorpos monoclonais anti-CD11b, anti-CD45, anti-CD90 e anti-CD105, e de diferenciação celular em linhagem condrogênica, adipogênica e osteogênica. Houve diferenciação celular e marcação positiva dos antígenos de superfície CD44 e MHCI e negativa dos CD45 e CD11b, confirmando-se a origem mesenquimal e não hematopoiética. A viabilidade celular média obtida das células-tronco mesenquimais foi de 92,83% considerada satisfatória. Portanto, a utilização deste protocolo para obtenção de células-tronco mesenquimais do tecido adiposo se mostrou uma opção confiável e viável para utilização na terapia celular em equinos. _________________________________________________________________________________ ABSTRACTDue to the excellent results of stem cell research and its promising applicability, the therapy with these cells is undergoing a period of maximum visibility and numerous studies. In order to standardize the culture and to evaluate the viability of mesenchymal stem cells from adipose tissue of horses, aiming at cell therapy in this species, a study of isolation, expansion and characterization was conducted. For this, adipose tissue was collected from five crossbred horses aged approximately five years old. Subsequently, the tissue was immediately refrigerated and transported to the laboratory where the isolation and culture protocol, lasting approximately 14 days, was performed. After the end of this procedure, a cell pellet was obtained and resuspended in PBS. A portion of material obtained by centrifugation was collected and the cells were stained with 1% Trypan Blue for quantification and assessment of cell viability. Another sample was collected to confirm the lineage of stem cells by flow cytometry using monoclonal against the surface markers CD11b, CD45, CD90 and CD105; and by cellular differentiation assays to chondrogenic, adipogenic and osteogenic lineages. The isolated cells differentiated in the three lineages and showed positive staining for CD90 and CD105 and negative staining for CD11b and CD45, confirming the isolation of stem cells of mesenchymal origin. The average cell viability obtained was 92.83% and thus satisfactory. Therefore, the use of this protocol to obtain mesenchymal stem cells from adipose tissue was deemed to be a promising option for cell therapy in horses

Association between left ventricular diastolic dysfunction and severity of chronic obstructive pulmonary disease

OBJECTIVES: The prevalence of electrocardiographic and echocardiographic abnormalities in chronic obstructive pulmonary disease according to disease severity has not yet been established. The aim of this study was to assess the prevalence of electrocardiographic and echocardiographic abnormalities in chronic obstructive pulmonary disease patients according to disease severity. METHODS: The study included 25 mild/moderate chronic obstructive pulmonary disease patients and 25 severe/very severe chronic obstructive pulmonary disease patients. All participants underwent clinical evaluation, spirometry and electrocardiography/echocardiography. RESULTS: Electrocardiography and echocardiography showed Q-wave alterations and segmental contractility in five (10%) patients. The most frequent echocardiographic finding was mild left diastolic dysfunction (88%), independent of chronic obstructive pulmonary disease stage. The proportion of right ventricular overload (

Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis

The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively

Escore de risco para tromboembolismo e prevenção em pacientes hospitalizados em uma clínica cirúrgica

Objetivo: Identificar o risco tromboembolítico em pacientes cirúrgicos e o uso das medidas preventivas. Método: Estudo epidemiológico transversal, retrospectivo por meio de entrevistas e abordagem aos prontuários de pacientes cirúrgicos. O risco para tromboembolismo foi avaliado, conforme escala validada e adaptada. As análises foram processadas pelo software estatístico Stata versão 12.1. Resultados: Foram entrevistados e analisados os prontuários de 100 pacientes. Todos os participantes apresentaram pelo menos um fator de risco para tromboembolismo. Dos entrevistados, 41% foram classificados como de alto risco, 7% receberam tromboprofilaxia medicamentosa e 11% recebendo profilaxia não medicamentosa. Conclusão: Apesar de todos os participantes apresentarem pelo menos um fator de risco para tromboembolismo venoso, a implementação de medidas preventivas foi baixa indicando a necessidade de múltiplas intervenções, tais como implementação de protocolos, educação permanente - principalmente da equipe de enfermagem, uso de ferramentas de monitoramento e auditoria de resultados