A PIV-Guided Large-Eddy Simulation of In-Cylinder Flows

A combination of Large-Eddy Simulation (LES) and Particle Image Velocimetry (PIV) was utilized to investigate the three-dimensional in-cylinder flow within an optically accessible Direct Injection Spark Ignition (DISI) engine at motored engine operation. The PIV measurements were used to guide the meshing procedure by identifying the regions were refinements and improvements were needed. From the iteratively optimized meshes LES results are shown from two selected meshes, an intermediate coarse mesh and the final optimized mesh, and compared to PIV measurements. The evolution of the intake flow and the tumble in the central tumble plane during compression are presented and discussed. Exploitation of the LES results allowed showing the influence of out-of-plane velocities along the cylinder liner impacting the formation of the tumble flow. The optimized mesh was then used to investigate the influence of the spark plug on the in-cylinder flow. For the studied engine the spark plug had a significant impact on the evolution of the tumble flow during compression. Finally 35 engine cycles were simulated using the optimized mesh with the spark plug in place. Velocity distributions in a region below the spark plug are shown and compared with PIV results. The two-sample Kolmogorov-Smirnov test revealed a strong similarity between the velocity distributions obtained by PIV and LES, thus validating the potential of LES for investigating cycle-to-cycle variability

Le logiciel libre dans la gestion de projet IT

L’objectif de ce travail de diplôme est d’explorer le monde du logiciel libre, de tester des logiciels libres de gestion de projet et d’avoir l’avis d’entreprises ayant fait la démarche d’utiliser des logiciels libres. Ce mémoire permettra à tous les chefs de projet, qui recherchent un logiciel libre de gestion de projet, d’avoir une description et une étude détaillée de sept logiciels libres de gestion de projet. Ils choisiront le logiciel qui sied le mieux à leurs besoins. De plus, l’analyse facilite au maximum l’utilisation du logiciel choisi. Vous trouverez aussi le compte rendu des entreprises cibles : Hortis, le CTI et LynuxSolutions qui m’ont permis de les interviewer. Les thèmes concernant l’historique, le business et les logiciels libres de l’entreprise, ainsi que la mise en place, l’attente et la sécurité des logiciels libres seront abordés. Il est essentiel que le lecteur utilise cet ouvrage s’il désire se familiariser avec un des sept logiciels libres de gestion de projet qui a passé le test de l’analyse multicritère* avec pondération par matrice de préférence*

Biodistribution rapide d'OMV (Outer-Membrane Vesicles) de l'intestin vers des organes via le sang chez la souris

International audienceA cell’s ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type of EVs called outer membrane vesicles (OMVs). We previously observed the presence of OMVs in human blood, which could represent a means of communication from the microbiota to the host. Here, in order to investigate the possible translocation of OMVs from the intestine to other organs, the mouse was used as an animal model after OMVs administration. To achieve this, we first optimized the signal of OMVs containing the fluorescent protein miRFP713 associated with the outer membrane anchoring peptide OmpA by adding biliverdin, a fluorescence cofactor, to the cultures. The miRFP713-expressing OMVs produced in E. coli REL606 strain were then characterized according to their diameter and protein composition. Native- and miRFP713-expressing OMVs were found to produce homogenous populations of vesicles. Finally, in vivo and ex vivo fluorescence imaging was used to monitor the distribution of miRFP713-OMVs in mice in various organs whether by intravenous injection or oral gavage. The relative stability of the fluorescence signals up to 3 days post-injection/gavage paves the way to future studies investigating the OMV-based communication established between the different microbiotas and their host

Genetic dissection of sodium and potassium transport along the aldosterone-sensitive distal nephron: Importance in the control of blood pressure and hypertension.

In this review, we discuss genetic evidence supporting Guyton's hypothesis stating that blood pressure control is critically depending on fluid handling by the kidney. The review is focused on the genetic dissection of sodium and potassium transport in the distal nephron and the collecting duct that are the most important sites for the control of sodium and potassium balance by aldosterone and angiotensin II. Thanks to the study of Mendelian forms of hypertension and their corresponding transgenic mouse models, three main classes of diuretic receptors (furosemide, thiazide, amiloride) and the main components of the aldosterone- and angiotensin-dependent signaling pathways were molecularly identified over the past 20years. This will allow to design rational strategies for the treatment of hypertension and for the development of the next generation of diuretics

Low incidence of SARS-CoV-2, risk factors of mortality and the course of illness in the French national cohort of dialysis patients

International audienceThe aim of this study was to estimate the incidence of COVID-19 disease in the French national population of dialysis patients, their course of illness and to identify the risk factors associated with mortality. Our study included all patients on dialysis recorded in the French REIN Registry in April 2020. Clinical characteristics at last follow-up and the evolution of COVID-19 illness severity over time were recorded for diagnosed cases (either suspicious clinical symptoms, characteristic signs on the chest scan or a positive reverse transcription polymerase chain reaction) for SARS-CoV-2. A total of 1,621 infected patients were reported on the REIN registry from March 16th, 2020 to May 4th, 2020. Of these, 344 died. The prevalence of COVID-19 patients varied from less than 1% to 10% between regions. The probability of being a case was higher in males, patients with diabetes, those in need of assistance for transfer or treated at a self-care unit. Dialysis at home was associated with a lower probability of being infected as was being a smoker, a former smoker, having an active malignancy, or peripheral vascular disease. Mortality in diagnosed cases (21%) was associated with the same causes as in the general population. Higher age, hypoalbuminemia and the presence of an ischemic heart disease were statistically independently associated with a higher risk of death. Being treated at a selfcare unit was associated with a lower risk. Thus, our study showed a relatively low frequency of COVID-19 among dialysis patients contrary to what might have been assumed