Expanding Glycomic Investigations Through Thiol-derivatized Glycans

N-(2-thioethyl)-2-aminobenzamide (TEAB), a novel glycan auxiliary, was synthesized and its utility was evaluated. The auxiliary was conjugated to glycans by reductive amination with the water-stable reagent 2-picoline borane complex. Glycan products, which ranged from 1 to 7 linked hexoses, were all isolated in yields ranging from 60% to 90% after purification by reverse-phase chromatography. The novel conjugate introduces a convenient, shelf-stable thiol directly onto the desired free glycans with purification advantages and direct modification with efficient reactions through alkenes, halides, epoxides, disulfides, and carboxylates in yields of 49% to 93%. Subsequently, a thiol-selective modification of the BSA protein was used to generate a neoglycoprotein with a bifunctional PEG–maleimide linker. To further illustrate the utility of a thiol motif, 2-thiopyridine activation of a thiol-containing support facilitated the covalent chromatographic purification of labeled glycans in yields up to 63%. Additionally, initial proof of concept of implementation in a light printed microarray was explored and validated through FITC-labeled concanavalin A binding. The thiol-functionalized glycans produced greatly expand the diversity of bioconjugation tools that can be developed with glycans and enable a variety of biological investigations. Finally, some non-reductive amination techniques were preliminarily investigated which achieved labeling of a xylose species in non-anomeric positions in poor yields

Information in vertical blanking interval of video sync signal

In a television sync signal one or more of the post-equalizing pulses of the vertical blanking interval is/are altered in duration to provide information to a receiving circuit

Mapping Old St. Joseph, Its Railroads, and Environs

Many people have heard the story of the old city of St. Joseph, which existed between 1836 and 1844 in the area of the present city of Port St. Joe, on the Gulf of Mexico, a score of miles from the mouth of the Apalachicola River. The story of St. Joseph has been told in many places and in conflicting forms. Novels and scholarly articles have been written about the city and the story of the destruction by fever and flood of “the wickedest city in the South” has been told in many sermons. Much of this legend is false, belonging to folklore rather than history

Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions

Citation: Hurst, M. N., & DeLong, R. K. (2016). Two-Dimensional Fluorescence Difference Spectroscopy to Characterize Nanoparticles and their Interactions. Scientific Reports, 6, 9. https://doi.org/10.1038/srep33287Two dimensional fluorescence difference spectroscopy (2D FDS) detects nanoparticle interactions following surface functionalization and biomolecule loading by generating a spectral signature of the fluorescent intensity per excitation and emission wavelengths. Comparing metal oxide nanoparticles revealed a unique spectral signature per material composition. 2D FDS showed to be sensitive to changes in surface properties between ZnO NPs synthesized by different methods. ZnO NP loaded with glycol chitosan, polyacrylic acid (PAA), or methoxy polyethylene glycol (mPEG) exhibited a distinct spectral signature shift. ZnO NP loaded with Torula Yeast RNA (TYRNA)(640 nm), polyinosinic: polycytidylic acid (pIC)(680 nm), or splice switching oligonucleotide (SSO)(650 nm) each revealed a shift in emission. Ras-Binding domain (RBD) at three concentrations (25, 37.5, 50 mu g/mL) showed that fluorescent intensity was inversely related to the concentration of protein loaded. These data support 2D FDS as a novel technique in identifying nanoparticles and their surface interactions as a quality assurance tool

Making progress in genetic kin recognition among vertebrates

A recent study in BMC Evolutionary Biology has shown that genetically similar individual ring-tailed lemurs are also more similar in their scent composition, suggesting a possible mechanism of kin recognition. Theoretical and experimental studies reveal challenges ahead in achieving a true systems-level understanding of this process and its outcomes

The contribution of small punch testing towards the development of materials for aero-engine applications

This paper, invited for presentation at the 33rd Meeting of the Spanish Group on Fracture and Structural Integrity, March 2016 in San Sebastian, Spain, reviews the recent work carried out in the authors’ laboratory, addressing the elucidation of tensile and creep characteristics of materials for aero engine components. Two specific applications of the Small Punch (SP) test assessment technology were identified, the first of these takes on board the unique potential of the SP test for testing small quantities of materials which are either in development or through their directional structure cannot easily be produced in quantities which would allow conventional mechanical testing. This goal also required the development and procurement of new SP test facilities capable of operation up to 1150 °C. The examples given in this paper are TiAl intermetallic alloys and nickel based single crystals, all studied utilising the Code of Practice for SP Creep Testing. The second application illustrates the use of SP testing to assess both the tensile and creep properties of additive layer manufactured (ALM) alloys such as IN718 and Ti-6Al-4V using the Code of Practice for SP Tensile and Fracture Testing. Due to the unavailability of sufficient material to facilitate conventional testing for comparison of materials property data, SP testing is unable to provide absolute data for all of these applications, nevertheless the ranking capabilities of SP testing are demonstrably proven

Metabolic trade-offs and the maintenance of the fittest and the flattest.

This is the post print version of the article, deposited in accordance with SHERPA RoMEO guidelines. The final definitive version is available from: http://www.nature.com/nature/journal/v472/n7343/full/nature09905.htmlHow is diversity maintained? Environmental heterogeneity is considered to be important, yet diversity in seemingly homogeneous environments is nonetheless observed. This, it is assumed, must either be owing to weak selection, mutational input or a fitness advantage to genotypes when rare. Here we demonstrate the possibility of a new general mechanism of stable diversity maintenance, one that stems from metabolic and physiological trade-offs. The model requires that such trade-offs translate into a fitness landscape in which the most fit has unfit near-mutational neighbours, and a lower fitness peak also exists that is more mutationally robust. The 'survival of the fittest' applies at low mutation rates, giving way to 'survival of the flattest' at high mutation rates. However, as a consequence of quasispecies-level negative frequency-dependent selection and differences in mutational robustness we observe a transition zone in which both fittest and flattest coexist. Although diversity maintenance is possible for simple organisms in simple environments, the more trade-offs there are, the wider the maintenance zone becomes. The principle may be applied to lineages within a species or species within a community, potentially explaining why competitive exclusion need not be observed in homogeneous environments. This principle predicts the enigmatic richness of metabolic strategies in clonal bacteria and questions the safety of lethal mutagenesis as an antimicrobial treatment

An Analysis of Method Books for the Bass Trombone

This paper provides a survey and analysis of eight method books for the bass trombone. Robert G. Hurst describes each of these titles with detail and explores the methods discussed in the texts, as well as the history and construction of the bass trombone