Resolving SNR 0540-6944 from LMC X-1 with Chandra

We examine the supernova remnant (SNR) 0540-697 in the Large Magellanic Cloud (LMC) using data from the Chandra ACIS. The X-ray emission from this SNR had previously been hidden in the bright emission of nearby X-ray binary LMC X-1; however, new observations with Chandra can finally reveal the SNR's structure and spectrum. We find the SNR to be a thick-shelled structure about 19 pc in diameter, with a brightened northeast region. Spectral results suggest a temperature of 0.31 keV and an X-ray luminosity (0.3-3.0 keV) of 8.4 x 10^33 erg/s. We estimate an age of 12,000-20,000 yr for this SNR, but note that this estimate does not take into account the possibility of cavity expansion or other environmental effects.Comment: 8 pages, 2 GIF figures. Submitted to ApJL. Replaced with minor revisions from referee comment

A search for passive protoplanetary disks in the Taurus-Auriga star-forming region

We conducted a 12-month monitoring campaign of 33 T Tauri stars (TTS) in Taurus. Our goal was to monitor objects that possess a disk but have a weak Halpha line, a common accretion tracer for young stars, to determine whether they host a passive circumstellar disk. We used medium-resolution optical spectroscopy to assess the objects' accretion status and to measure the Halpha line. We found no convincing example of passive disks; only transition disk and debris disk systems in our sample are non-accreting. Among accretors, we find no example of flickering accretion, leading to an upper limit of 2.2% on the duty cycle of accretion gaps assuming that all accreting TTS experience such events. Combining literature results with our observations, we find that the reliability of traditional Halpha-based criteria to test for accretion is high but imperfect, particularly for low-mass TTS. We find a significant correlation between stellar mass and the full width at 10 per cent of the peak (W10%) of the Halpha line that does not seem to be related to variations in free-fall velocity. Finally, our data reveal a positive correlation between the Halpha equivalent width and its W10%, indicative of a systematic modulation in the line profile whereby the high-velocity wings of the line are proportionally more enhanced than its core when the line luminosity increases. We argue that this supports the hypothesis that the mass accretion rate on the central star is correlated with the Halpha W10% through a common physical mechanism.Comment: accepted for publication in MNRAS; 26 pages, 9 figures, 3 table

An Optical Study of Stellar and Interstellar Environments of Seven Luminous and Ultraluminous X-Ray Sources

We have studied the stellar and interstellar environments of two luminous X-ray sources and five ultraluminous X-ray sources (ULXs) in order to gain insight into their nature. Archival Hubble Space Telescope images were used to identify the optical counterparts of the ULXs Ho IX X-1 and NGC 1313 X-2, and to make photometric measurements of the local stellar populations of these and the luminous source IC 10 X-1. We obtained high-dispersion spectroscopic observations of the nebulae around these seven sources to search for He II lambda-4686 emission and to estimate the expansion velocities and kinetic energies of these nebulae. Our observations did not detect nebular He II emission from any source, with the exception of LMC X-1; this is either because we missed the He III regions or because the nebulae are too diffuse to produce He II surface brightnesses that lie within our detection limit. We compare the observed ionization and kinematics of the supershells around the ULXs Ho IX X-1 and NGC 1313 X-2 with the energy feedback expected from the underlying stellar population to assess whether additional energy contributions from the ULXs are needed. In both cases, we find insufficient UV fluxes or mechanical energies from the stellar population; thus these ULXs may be partially responsible for the ionization and energetics of their supershells. All seven sources we studied are in young stellar environments and six of them have optical counterparts with masses >~7 M_sun; thus, these sources are most likely high-mass X-ray binaries.Comment: 30 pages, 9 figures. Numerous minor revisions, primarily to more accurately cite earlier work by Pakull and Mirioni, and to correct typographical errors. Removed a misleading sentence in the Introduction (re: X-ray photoionization by ULXs). Accepted for publication in The Astrophysical Journal. Figures have been reduced in resolution for space requirements; full-resolution figures may be requested by email to [email protected]

An Optical Study of Stellar and Interstellar Environments of Seven Luminous and Ultraluminous X-Ray Sources

We have studied the stellar and interstellar environments of two luminous X-ray sources and five ultraluminous X-ray sources (ULXs) in order to gain insight into their nature. Archival Hubble Space Telescope images were used to identify the optical counterparts of the ULXs Ho IX X-1 and NGC 1313 X-2, and to make photometric measurements of the local stellar populations of these and the luminous source IC 10 X-1. We obtained high-dispersion spectroscopic observations of the nebulae around these seven sources to search for He II lambda-4686 emission and to estimate the expansion velocities and kinetic energies of these nebulae. Our observations did not detect nebular He II emission from any source, with the exception of LMC X-1; this is either because we missed the He III regions or because the nebulae are too diffuse to produce He II surface brightnesses that lie within our detection limit. We compare the observed ionization and kinematics of the supershells around the ULXs Ho IX X-1 and NGC 1313 X-2 with the energy feedback expected from the underlying stellar population to assess whether additional energy contributions from the ULXs are needed. In both cases, we find insufficient UV fluxes or mechanical energies from the stellar population; thus these ULXs may be partially responsible for the ionization and energetics of their supershells. All seven sources we studied are in young stellar environments and six of them have optical counterparts with masses >~7 M_sun; thus, these sources are most likely high-mass X-ray binaries.Comment: 30 pages, 9 figures. Numerous minor revisions, primarily to more accurately cite earlier work by Pakull and Mirioni, and to correct typographical errors. Removed a misleading sentence in the Introduction (re: X-ray photoionization by ULXs). Accepted for publication in The Astrophysical Journal. Figures have been reduced in resolution for space requirements; full-resolution figures may be requested by email to [email protected]

Does the Debris Disk around HD 32297 Contain Cometary Grains?

We present an adaptive optics imaging detection of the HD 32297 debris disk at L' (3.8 \microns) obtained with the LBTI/LMIRcam infrared instrument at the LBT. The disk is detected at signal-to-noise per resolution element ~ 3-7.5 from ~ 0.3-1.1" (30-120 AU). The disk at L' is bowed, as was seen at shorter wavelengths. This likely indicates the disk is not perfectly edge-on and contains highly forward scattering grains. Interior to ~ 50 AU, the surface brightness at L' rises sharply on both sides of the disk, which was also previously seen at Ks band. This evidence together points to the disk containing a second inner component located at $\lesssim$ 50 AU. Comparing the color of the outer (50 $< r$/AU $< 120$) portion of the disk at L' with archival HST/NICMOS images of the disk at 1-2 \microns allows us to test the recently proposed cometary grains model of Donaldson et al. 2013. We find that the model fails to match the disk's surface brightness and spectrum simultaneously (reduced chi-square = 17.9). When we modify the density distribution of the model disk, we obtain a better overall fit (reduced chi-square = 2.9). The best fit to all of the data is a pure water ice model (reduced chi-square = 1.06), but additional resolved imaging at 3.1 \microns is necessary to constrain how much (if any) water ice exists in the disk, which can then help refine the originally proposed cometary grains model.Comment: Accepted to ApJ January 13, 2014. 12 pages (emulateapj style), 9 figures, 1 tabl

Requisite Roles of A 2A Receptors, Nitric Oxide, and K ATP Channels in Retinal Arteriolar Dilation in Response to Adenosine

PURPOSE. Adenosine is a potent vasodilator of retinal microvessels and is implicated to be a major regulator of retinal blood flow during metabolic stress. However, the receptor subtypes and the underlying signaling mechanism responsible for the dilation of retinal microvessels in response to adenosine remain unclear. In the present study, the roles of specific adenosine receptor subtypes, nitric oxide (NO), and adenosine triphosphate (ATP)-sensitive K ϩ (K ATP ) channels in adenosineinduced dilation of retinal arterioles in vitro were examined. METHODS. Porcine second-order retinal arterioles (40 -70 m in internal diameter) were isolated, cannulated, and pressurized to 55 cmH 2 O luminal pressure without flow. Diameter changes in response to agonists were recorded by using videomicroscopic techniques. RESULTS. All vessels exhibited basal tone and dilated dose dependently in reaction to adenosine, N 6 -cyclopentyladenosine (an adenosine A 1 receptor agonist), and 2-[p-(2-carboxyethyl)]phenylethyl-amino-5Ј-N-ethylcarboxamidoadenosine (CGS21680; an adenosine A 2A receptor agonist). These responses were not altered by the selective adenosine A 1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine, but were significantly attenuated by the selective adenosine A 2A receptor antagonist 4-(2-{7-amino-2-(2-furyl)[1,2,4]-triazolo[2,3-a] [1,3,5]triazin-5-ylamino}ethyl)phenol. Blockade of NO synthase, but not of cyclooxygenase or cytochrome P-450 epoxygenase, significantly attenuated the vasodilations in response to adenosine and CGS21680. The residual vasodilative reactions to both agonists was nearly abolished by the K ATP channel inhibitor glibenclamide. CONCLUSIONS. These data suggest that adenosine evokes retinal arteriolar dilation via activation of A 2A receptors and subsequent production of NO and opening of K ATP channels. A better understanding of the fundamental signaling pathways responsible for adenosine-induced dilation of retinal arterioles may help shed light on the possible mechanisms contributing to impaired retinal blood flow regulation in patients after retinal ischemia. (Invest Ophthalmol Vis Sci. 2005;46:2113-211

Bringing "The Moth" to Light: A Planet-Sculpting Scenario for the HD 61005 Debris Disk

The HD 61005 debris disk ("The Moth") stands out from the growing collection of spatially resolved circumstellar disks by virtue of its unusual swept-back morphology, brightness asymmetries, and dust ring offset. Despite several suggestions for the physical mechanisms creating these features, no definitive answer has been found. In this work, we demonstrate the plausibility of a scenario in which the disk material is shaped dynamically by an eccentric, inclined planet. We present new Keck NIRC2 scattered-light angular differential imaging of the disk at 1.2-2.3 microns that further constrains its outer morphology (projected separations of 27-135 AU). We also present complementary Gemini Planet Imager 1.6 micron total intensity and polarized light detections that probe down to projected separations less than 10 AU. To test our planet-sculpting hypothesis, we employed secular perturbation theory to construct parent body and dust distributions that informed scattered-light models. We found that this method produced models with morphological and photometric features similar to those seen in the data, supporting the premise of a planet-perturbed disk. Briefly, our results indicate a disk parent body population with a semimajor axis of 40-52 AU and an interior planet with an eccentricity of at least 0.2. Many permutations of planet mass and semimajor axis are allowed, ranging from an Earth mass at 35 AU to a Jupiter mass at 5 AU.Comment: Accepted to AJ; added Figure 5 and minor text edit

Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum

https://link.springer.com/article/10.1007/s00253-022-12182-9[EN] The fungus Trichoderma arundinaceum exhibits biological control activity against crop diseases caused by other fungi. Two mechanisms that likely contribute to this activity are upregulation of plant defenses and production of two types of antifungal secondary metabolites: the sesquiterpenoid harzianum A (HA) and the polyketide-derived aspinolides. The goal of the current study was to identify aspinolide biosynthetic genes as part of an effort to understand how these metabolites contribute to the biological control activity of T. arundinaceum. Comparative genomics identified two polyketide synthase genes (asp1 and asp2) that occur in T. arundinaceum and Aspergillus ochraceus, which also produces aspinolides. Gene deletion and biochemical analyses in T. arundinaceum indicated that both genes are required for aspinolide production: asp2 for formation of a 10-member lactone ring and asp1 for formation of a butenoyl subsituent at position 8 of the lactone ring. Gene expression and comparative genomics analyses indicated that asp1 and asp2 are located within a gene cluster that occurs in both T. arundinaceum and A. ochraceus. A survey of genome sequences representing 35 phylogenetically diverse Trichoderma species revealed that intact homologs of the cluster occurred in only two other species, which also produced aspinolides. An asp2 mutant inhibited fungal growth more than the wild type, but an asp1 mutant did not, and the greater inhibition by the asp2 mutant coincided with increased HA production. These findings indicate that asp1 and asp2 are aspinolide biosynthetic genes and that loss of either aspinolide or HA production in T. arundinaceum can be accompanied by increased production of the other metabolite(s).SIPublicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCL

Advection-Dominated Accretion Model of Sagittarius A*: Evidence for a Black Hole at the Galactic Center

Sgr A* at the Galactic Center is a puzzling source. It has a mass M=(2.5+/-0.4) x 10^6 solar masses which makes it an excellent black hole candidate. Observations of stellar winds and other gas flows in its vicinity suggest a mass accretion rate approximately few x 10^{-6} solar masses per year. However, such an accretion rate would imply a luminosity > 10^{40} erg/s if the radiative efficiency is the usual 10 percent, whereas observations indicate a bolometric luminosity <10^{37} erg/s. The spectrum of Sgr A* is unusual, with emission extending over many decades of wavelength. We present a model of Sgr A* which is based on a two-temperature optically-thin advection-dominated accretion flow. The model is consistent with the estimated mass and accretion rate, and fits the observed fluxes in the cm/mm and X-ray bands as well as upper limits in the sub-mm and infrared bands; the fit is less good in the radio below 86 GHz and in gamma-rays above 100 MeV. The very low luminosity of Sgr A* is explained naturally in the model by means of advection. Most of the viscously dissipated energy is advected into the central mass by the accreting gas, and therefore the radiative efficiency is extremely low, approximately 5 x 10^{-6}. A critical element of the model is the presence of an event horizon at the center which swallows the advected energy. The success of the model could thus be viewed as confirmation that Sgr A* is a black hole.Comment: 41 pages (Latex) including 6 Figures and 2 Tables. Final Revised Version changes to text, tables and figures. ApJ, 492, in pres

T-cell subpopulations αβ and γδ in cord blood of very preterm infants : The influence of intrauterine infection

Open Access: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are creditedPreterm infants are very susceptible to infections. Immune response mechanisms in this group of patients and factors that influence cord blood mononuclear cell populations remain poorly understood and are considered insufficient. However, competent immune functions of the cord blood mononuclear cells are also described. The aim of this work was to evaluate the T-cell population (CD3+) with its subpopulations bearing T-cell receptor (TCR) αβ or TCR γδ in the cord blood of preterm infants born before 32 weeks of gestation by mothers with or without an intrauterine infection. Being a pilot study, it also aimed at feasibility check and assessment of an expected effect size. The cord blood samples of 46 infants age were subjected to direct immunofluorescent staining with monoclonal antibodies and then analyzed by flow cytometry. The percentage of CD3+ cells in neonates born by mothers with diagnosis of intrauterine infection was significantly lower than in neonates born by mothers without infection (p = 0.005; Mann-Whitney U test). The number of cells did not differ between groups. Infection present in the mother did not have an influence on the TCR αβ or TCR γδ subpopulations. Our study contributes to a better understanding of preterm infants' immune mechanisms, and sets the stage for further investigations.Peer reviewedFinal Published versio