Sister chromatid cohesion establishment occurs in concert with lagging strand synthesis

Cohesion establishment is central to sister chromatid tethering reactions and requires Ctf7/Eco1-dependent acetylation of the cohesin subunit Smc3. Ctf7/Eco1 is essential during S phase, and a number of replication proteins (RFC complexes, PCNA and the DNA helicase Chl1) all play individual roles in sister chromatid cohesion. While the mechanism of cohesion establishment is largely unknown, a popular model is that Ctf7/Eco1 acetylates cohesins encountered by and located in front of the fork. In turn, acetylation is posited both to allow fork passage past cohesin barriers and convert cohesins to a state competent to capture subsequent production of sister chromatids. Here, we report evidence that challenges this pre-replicative cohesion establishment model. Our genetic and biochemical studies link Ctf7/Eco1 to the Okazaki fragment flap endonuclease, Fen1. We further report genetic and biochemical interactions between Fen1 and the cohesion-associated DNA helicase, Chl1. These results raise a new model wherein cohesin deposition and establishment occur in concert with lagging strand-processing events and in the presence of both sister chromatids

Pennsylvania\u27s Family Caregiver Support Program: A Demonstration Project

The physical, emotional, and economic burdens of family caregiving can present a serious threat to the stability and continuity of a caregiving situation. Public policymakers, aware of the high costs of replacing such voluntary efforts with publicly funded institutional care, are becoming more and more concerned about the needs of caregivers and possible intervention strategies to meet those needs. This article begins with a description of Pennsylvania\u27s new policy initiative for caregivers, the Family Caregiver Support Program (FCSP). Following is a discussion of the evaluation of the program\u27s demonstration phase by the Human Organization Science Institute of Villanova University. The evaluation concluded that the FCSP has a significant positive impact on the lives and abilities of caregivers, including the reduction of caregiver stress and burden. The concluding summary of program results seeks to sharpen the reader\u27s interest in the potential benefits of an intervention strategy such as this and suggests a need for additional research for the benefit of those concerned about health care cost containment

Making the great transformation, November 13, 14, and 15, 2003

This repository item contains a single issue of the Pardee Conference Series, a publication series that began publishing in 2006 by the Boston University Frederick S. Pardee Center for the Study of the Longer-Range Future. This Conference took place during November 13, 14, and 15, 2003. Co-organized by Cutler Cleveland and Adil Najam.The conference discussants and participants analyze why transitions happen, and why they matter. Transitions are those wide-ranging changes in human organization and well being that can be convincingly attributed to a concerted set of choices that make the world that was significantly and recognizably different from the world that becomes. Transition scholars argue that that history does not just stumble along a pre-determined path, but that human ingenuity and entrepreneurship have the ability to fundamentally alter its direction. However, our ability to ‘will’ such transitions remains in doubt. These doubts cannot be removed until we have a better understanding of how transitions work

Economic development, human development, and the pursuit of happiness, April 1, 2, and 3, 2004

This repository item contains a single issue of the Pardee Conference Series, a publication series that began publishing in 2006 by the Boston University Frederick S. Pardee Center for the Study of the Longer-Range Future. This was the Center's spring conference, which took place during April 1, 2, and 3, 2004.The conference asks the questions, how can we make sure that the benefits of economic growth flow into health, education, welfare, and other aspects of human development; and what is the relationship between human development and economic development? Speakers and participants discuss the role that culture, legal and political institutions, the UN Developmental Goals, the level of decision-making, and ethics, play in development

The ‘T-Shaped Buyer’: a transactional perspective on supply chain relationships

This paper challenges the normative view of interdependent buyer-seller relationships and provides a more holistic perspective of the contextual reality that shapes buyer behaviour. By proposing an innovative qualitative methodology, which focusses on boundary-spanning, pre-sales interactions, the research penetrates complex and commercially sensitive buyer-seller relationships. The longitudinal research design uses web-based diaries and follow-up interviews to explore conditions of power based interdependence between buyers and sellers. The ensuing data is mapped using qualitative content analysis and the results are aggregated graphically for assessment. Using this approach the study develops a nuanced view of the dominant patterns of buyer behaviour, and challenges the opinion that a search for competitive advantage will strengthen cooperative relationships in conditions of power based interdependence. The paper introduces the metaphor of the 'T-Shaped Buyer' to explain the empirical findings and, while acknowledging the contextual limits of the study, suggests that this metaphor may cause both academics and practitioners to reflect on normative thinking

Concepts and Measurement in Multimethod Research

This article argues that concept misformation and conceptual stretching undermine efforts to combine qualitative and quantitative methods in multimethod research (MMR). Two related problems result from the mismatch of qualitatively and quantitatively construed concepts. Mechanism muddling occurs when differences in the connotation of qualitatively and quantitatively construed concepts embed different causal properties into conceptual definitions. Conceptual slippage occurs when qualitatively and quantitatively construed concepts use incompatible nominal, ordinal, or radial scales. Instead of gaining leverage from the synthesis of large- and small-N analysis, these problems can push MMR in two diametrically opposed directions, emphasizing one methodological facet at the cost of the other.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

"Who's ready for the cave?" Thailand's Tham Luang Rescue Museum as teaching case study

This article demonstrates how a teaching case study approach can benefit museum studies courses. It takes as its focus the widely reported rescue of a boys’ football team from a flooded cave in Thailand (2018). The success of this multinational effort led to immediate calls for the establishment of a “living museum” dedicated to remembering what became known as the “Tham Luang cave rescue.” Discussion of the potential form and function of this envisaged museum plus its wider ramifications formed a key component of a newly validated international MA program taught in the UK and China by local academics and practitioners. Issues addressed include collections management, interpretation and display as well as ethical considerations relating to funding and forward planning. These were debated through groupwork, student presentations and report writing. The article reflects on this experience and the lessons it has for studying museums. It advocates the use of other topical events as the basis for similar teaching case studies on the grounds that this best equips students with the knowledge and skills required in the workplace

Enforcement and Public Corruption: Evidence from US States

We use high-quality panel data on corruption convictions, new panels of assistant U.S. attorneys and relative public sector wages, and careful attention to the consequences of modeling endogeneity to estimate the impact of prosecutorial resources on criminal convictions of those who undertake corrupt acts. Consistent with system capacity arguments, we find that greater prosecutor resources result in more convictions for corruption, other things equal. We find more limited, recent evidence for the deterrent effect of increased prosecutions. We control for and confirm in a panel context the effects of many previously identified correlates and causes of corruption. By explicitly determining the allocation of prosecutorial resources endogenously from past corruption convictions and political considerations, we show that this specification leads to larger estimates of the effect of resources on convictions. The results are robust to various ways of measuring the number of convictions as well as to various estimators

Political and Judicial Checks on Corruption: Evidence from American State Governments

The paper investigates the effects of checks and balances on corruption. Within a presidential system, effective separation of powers is achieved under divided government, with the executive and legislative branches being controlled by different political parties. When government is unified, no effective separation exists even within a presidential system, but, we argue, can be partially restored by having an accountable judiciary. Our empirical findings show that divided government and elected, rather than appointed, state supreme court judges are associated with lower corruption and, furthermore, that the effect of an accountable judiciary is stronger under unified government, where government cannot control itself. The effect of an accountable judiciary seems to be driven primarily by judges chosen through direct elections, rather than those exposed to a retention vote following appointment