The hydrolysis of proteins

Temperature effects on hydrolysis reaction in protein amino acid

Gas-liquid chromatography of amino acids in biological substances

Developing gas-liquid chromatographic method for quantitative analysis of amino acids in blood plasma and urin

Using the local gyrokinetic code, GS2, to investigate global ITG modes in tokamaks. (I) s-α{\alpha} model with profile and flow shear effects

This paper combines results from a local gyrokinetic code with analytical theory to reconstruct the global eigenmode structure of the linearly unstable ion-temperature-gradient (ITG) mode with adiabatic electrons. The simulations presented here employ the s-${\alpha}$ tokamak equilibrium model. Local gyrokinetic calculations, using GS2 have been performed over a range of radial surfaces, x, and for ballooning phase angle, p, in the range -${\pi} {\leq} p {\leq\pi}$, to map out the complex local mode frequency, ${\Omega_0(x, p) = \omega_0(x, p) + i\gamma_0(x, p)}$. Assuming a quadratic radial profile for the drive, namely ${\eta_i = L_n/L_T}$, (holding constant all other equilibrium profiles such as safety factor, magnetic shear etc.), ${\Omega_0(x, p)}$ has a stationary point. The reconstructed global mode then sits on the outboard mid plane of the tokamak plasma, and is known as a conventional or isolated mode, with global growth rate, ${\gamma}$ ~ Max[${\gamma_0(x, p)}$], where ${\gamma_0(x, p)}$ is the local growth rate. Taking the radial variation in other equilibrium profiles (e.g safety factor q(x)) into account, removes the stationary point in ${\Omega_0(x, p)}$ and results in a mode that peaks slightly away from the outboard mid-plane with a reduced global growth rate. Finally, the influence of flow shear has also been investigated through a Doppler shift, ${\omega_0 \rightarrow \omega_0 + n\Omega^{\prime}x}$, where n is the toroidal mode number and ${\Omega^{\prime}}$ incorporates the effect of flow shear. The equilibrium profile variation introduces an asymmetry to the growth rate spectrum with respect to the sign of ${\Omega^{\prime}}$, consistent with recent global gyrokinetic calculations.Comment: 10 pages, 8 figures and 1 tabl

gamma-Aminobutyric acid inhibits synergistic interleukin-6 release but not transcriptional activation in astrocytoma cells

A decline in the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) may enhance cytokine release in Alzheimer\u27s disease (AD) resulting in neuroinflammation. We investigated the GABA-mediated suppression of the synergistic release of interleukin-6 (IL-6) due to interleukin 1-beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) synergy. Our results indicate that p38 and nuclear factor (NF)-kappaB are essential for the synergistic release of IL-6 by IL-1beta and TNFalpha. Both IL-1beta and TNF-alpha stimulate p38 phosphorylation and each are able to enhance the degradation of the NF-kappaB inhibitor, IkappaB-alpha, with no change in IkappaB-beta. Interestingly, co-treatments IL-1beta and TNF-alpha induce a greater increase in IkappaB-alpha degradation compared to either cytokine alone. In addition, co-treatment increased IL-6 mRNA indicating synergy on a transcriptional level. In addition, Western blot analysis revealed that co-treatments of IL-1beta and TNF-alpha resulted in an increase of IL-6 protein that can be reversed by GABA. Furthermore, co-treatments of IL-1beta and TNF-alpha resulted in activation of p38 and degradation of IkappaB-alpha at earlier time points compared to either cytokine alone. These data suggests that IL-1beta and TNF-alpha synergizes by accelerating p38 and NF-kappaB activation, increasing IL-6 transcription. Although GABA suppressed the IL-1beta and TNF-alpha synergistic release of IL-6, it did not affect IkappaB-alpha or IkappaB-beta degradation, p38 phosphorylation or IL-6 transcription. Instead it appears that GABA may inhibit IL-6 translation. We have demonstrated that this synergistic release of IL-6 can be inhibited by GABA and its receptor agonists

Structure of Micro-instabilities in Tokamak Plasmas: Stiff Transport or Plasma Eruptions?

Solutions to a model 2D eigenmode equation describing micro-instabilities in tokamak plasmas are presented that demonstrate a sensitivity of the mode structure and stability to plasma profiles. In narrow regions of parameter space, with special plasma profiles, a maximally unstable mode is found that balloons on the outboard side of the tokamak. This corresponds to the conventional picture of a ballooning mode. However, for most profiles this mode cannot exist and instead a more stable mode is found that balloons closer to the top or bottom of the plasma. Good quantitative agreement with a 1D ballooning analysis is found provided the constraints associated with higher order profile effects, often neglected, are taken into account. A sudden transition from this general mode to the more unstable ballooning mode can occur for a critical flow shear, providing a candidate model for why some experiments observe small plasma eruptions (Edge Localised Modes, or ELMs) in place of large Type I ELMs.Comment: 11 pages, 3 figure

A study of ASRS reports involving general aviation and weather encounters

Consideration is given to the nature and characteristics of problems involving dissemination of weather information, use of this information by pilots, its adequacy for the purpose intended, the ability of the air traffic control system to cope with weather related incidents, and the various aspects of pilot behavior, aircraft equipment, and NAVAIDS affecting flights in which weather figures. It is concluded from the study that skill and training deficiencies of general aviation pilots are not major factors in weather related occurrences, nor is lack of aircraft equipment. Major problem causes are identified with timely and easily interpreted weather information, judgement and attitude factors of pilots, and the functioning of the air traffic control system

Ultrasound increases the aqueous extraction of phenolic compounds with high antioxidant activity from olive pomace

Olive pomace is a waste produced by the olive oil industry in massive quantities each year. Disposal of olive pomace is difficult due to high concentrations of phenolic compounds, which is an environmental concern. However, phenolic compounds have applications in the health industry. Therefore, extraction of phenolic compounds from olive pomace has the potential to remove an environmentally hazardous portion of pomace while creating an additional source of income for farmers and producers. Using advanced technologies including Ultrasound Assisted Extraction (UAE), combined with water as an extraction solvent, has recently gained popularity. The present study outlines the optimal UAE conditions for the extraction of phenolic compounds with high antioxidant activity from olive pomace. Optimal conditions were developed using RSM for parameters power, time and sample-to-solvent ratio. Total phenolic compounds determined by Folin Ciocalteu method and total major bioactive compounds determined by HPLC as well as antioxidant capacity (DPPH and CUPRAC) were investigated. The optimal conditions for the extraction of phenolic compounds with high antioxidant activity were 2 g of dried pomace/100 mL of water at 250 W power for 75 min. UAE improved the extraction efficiency of water and yielded extracts with high levels of phenolic compounds and strong antioxidant activity

A Comparative Study of International and American Study Abroad Students’ Expectations and Experiences with Host Countries in Selected Institutions of Higher Education

This was a comparative study of international and American study abroad students’ experiences and expectations with the host countries. The rationale for this study was to acquire a deeper understanding of different experiences of students who study abroad and to understand whether their expectations of the host country have an impact on their experiences. An opportunity sample of American study abroad and international students was selected from the United States student population and their expectations and experiences of the host country compared. The study addressed 6 research questions, using a mixed-method approach. The principal instrument for the investigation was the Cross-Cultural Participant Questionnaire conducted online. Associated hypotheses with the research questions were analyzed using Independent sample t-tests and Paired samples t-tests at an alpha level of .05 and the results were described using descriptive statistics. The open-ended questions were analyzed according to established qualitative techniques. The survey was completed by 421 respondents comprised of 155 international students, 252 American study abroad students, and 14 unknown labeled as others. The results of this study identified language fluency, building relationships with the host nationals, learning about a new culture, and personal change as significant expectations of the students. Overall, the students reported being satisfied with the services provided. International students were slightly more satisfied with access to support services than the American study abroad students. American study abroad students had experiences that closer matched their expectations of study abroad than was the case for international students