Description and molecular phylogeny of a new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal

A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central-Western Portugal), respectively. The new needle nematode P. lusitanicus n. sp. is characterised by a very large body size (8072–12,022 μm), an expanded and rounded lip region, ca 30 μm wide, with a clear constriction followed by a depression posterior to the amphidial aperture, amphidial fovea very large (11.0–19.0 μm), stirrup-shaped, with conspicuous slit-like aperture as shown in scanning electron microscopy studies, a very long and flexible odontostyle (180.0–223.0 μm), guiding ring located at 28.0–41.5 μm from anterior end, vulva anterior to the mid-body (34–41%), a dorsally convex-conoid tail with rounded terminus (29–42 μm long), bearing two or three pairs of caudal pores and males common (ratio 1:1.6 females) with spicules ca 80 μm long. Morphological and morphometric traits for P. plesioepimikis fit well with the original description, and is reported for the first time in Portugal. Integrative diagnosis of both species was completed with molecular data obtained using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA and partial 18S–rDNA. The phylogenetic relationships of these species with other Paralongidorus spp. using these three molecular markers indicated that P. lusitanicus n. sp. clustered together with other Paralongidorus spp. forming a sister clade with P. plesioepimikis, both of them sharing a large body, long odontostyle, an anteriorly located vulva and an expanded and rounded lip region with a clear constriction followed by a depression posterior to the amphidial aperture

Distributed Optical Fiber Sensing bonding techniques performance for embedment inside reinforced concrete structures

Distributed optical fiber sensors (DOFS) are modern-day cutting-edge monitoring tools that are quickly acquiring relevance in structural health monitoring engineering. Their most ambitious use is embedded inside plain or reinforced concrete (RC) structures with the scope of comprehending their inner-workings and the functioning of the concrete-reinforcement interaction. Yet, multiple studies have shown that the bonding technique with which the DOFS are bonded to the reinforcement bars has a significant role on the quality of the extracted strain data. Whilst this influence has been studied for externally bonded DOFS, it has not been done for embedded ones. The present article is set on performing such study by monitoring the strain measurement quality as sampled by DOFS bonded to multiple rebars with different techniques and adhesives. These instrumented rebars are used to produce differently sized RC ties later tested in tension. The discussion of the test outputs highlights the quasi-optimal performance of a DOFS/rebar bonding technique consisting of incising a groove in the rebar, positioning the DOFS inside it, bonding it with cyanoacrylate and later adding a protective layer of silicone. The resulting data is mostly noise-free and anomalies-free, yet still presents a newly diagnosed hitch that needs addressing in future research.The study was performed within project No 09.3.3-LMT-K-712-01-0145 that has received funding from European Social Fund under grant agreement with the Research Council of Lithuania (LMTLT). Furthermore, this work was supported by the Swedish Transportation Administration (Trafikverket) under the grant TRV/BBT 2017-028. The authors are also indebted to the Secretaria d’ Universitats i Recerca de la Generalitat de Catalunya for the funding provided through Agaur (2017 SGR 1481).Peer ReviewedPostprint (published version

Manifestaciones orales y periodontales de la enfermedad inflamatoria intestinal

La Enfermedad Inflamatoria Intestinal (EII) incluye la enfermedad de Crohn y la colitis ulcerosa. Numerosos estudios relacionan dichas afectaciones con la manifestación de determinadas lesiones orales. Respecto a la enfermedad periodontal algunos autores la correlacionan con la EII tanto en su prevalencia como en su grado de actividad. Se ha apuntado la alteración de los PMN como posible factor común en la patogénesis de ambas entidades. El propósito de nuestro artículo es revisar las manifestaciones orales que presentan los pacientes afectos de una EII, así como dilucidar su implicación con la enfermedad periodontal. Además se repasa el manejo del paciente odontológico con EII sometido a corticoterapia

Cavités circulaires enterrées (SICC) : étude de la flexibilité

National audienceLe développement actuel des communications induit de nombreuses contraintes sur les émetteurs/récepteurs et notamment sur les premiers éléments de la chaîne de réception. Ainsi les antennes, les LNAs et les filtres voient leurs spécifications se durcir. Les filtres par exemple doivent être fortement sélectifs, présenter peu de pertes et être bon marché tout en restant compacts. Les filtres en guide d'onde satisfont certaines de ces contraintes car ils présentent d'excellentes performances électriques, cependant, ils sont coûteux, lourds et encombrants. De plus, leur association à des circuits planaires est difficile. Les filtres planaires présentent une alternative bon marché, ils sont légers et faciles à réaliser. Malheureusement, les performances électriques de tels filtres sont limitées. La technique SIW (Substrate Integrated Waveguide) est une combinaison de ces deux technologies, elle a été introduite dans l'idée de tirer le meilleur de chacune d'elles : la compacité, le coût, la compatibilité et la facilité de réalisation pour les solutions planaires et les performances électriques pour les solutions volumiques. Cette technique consiste à délimiter une cavité remplie de diélectrique dans un substrat planaire. Pour ce faire, les murs électriques supérieurs et inférieurs sont obtenus grâce aux procédés classiques de métallisation. Les faces latérales sont quant à elles réalisées grâce à une ou plusieurs rangées de trous métallisés [1]-[3]. Les cavités résultantes sont habituellement rectangulaires, mais de nouvelles topologies circulaires ont été récemment décrites [4-5]. Elles sont appelées SICCs (Substrate Integrated Circular Cavities) et offrent une flexibilité plus importante que celle des cavités rectangulaires. Cette flexibilité permet non seulement de créer des topologies originales, mais aussi de raccourcir les lignes de transmission entre les divers composants de la chaîne de réception, et ce, afin de faciliter la conception du système et d'augmenter sa compacité. Le masque de l'une d'entre elles ainsi que les dimensions associées sont présentés sur la figure 1. Les réponses électriques simulées et mesurées sont présentées sur la figure 2. Nous proposons ici d'illustrer la flexibilité introduite par ces SICCs au travers de la conception de filtres d'ordre 3

BMS for Permit Vehicle Routing in Spain

The papers shows the main works developed in Spain for the construction of a Bridge Management System (BMS) to manage the vehicle permits over the bridges of the socalled Strategic Spanish Highway Network, i.e., the most important National highways. This network is owned by the National Government. The different construction times and particularities of the bridge sites results in a large number of different bridge types both in longitudinal (simple supported beam, continuous beam, frame, arch, etc.) and cross-sectional (precast I beams or spread box beams, concrete slabs, box-girder, etc.) configurations. Most of the bridges are quite new and very well documented, but also a huge number of older bridges are present whose data (design drawings, material strength, etc.) is not available. The work is summarized in a Bridge Management System (BMS) installed on a PC computer that in a quick and automatic way performs the structural analysis of the bridges crossed by a defined special permit and compares the results with the maximum allowable actions over the bridge. The final result is the authorization or no authorization. In the paper are discussed the theoretical background used, the design philosophy and the implementation of such a system in Spain. The criteria and methodology may be extrapolated to similar cases in other countries. After some time in operation, the software developed has been shown as a useful tool to assist in the decision-making process for the bridge engineers of the Road Directorate in Spain.Peer Reviewe

The atlas of unburnable oil for supply-side climate policies

To limit the increase in global mean temperature to 1.5 °C, CO2 emissions must be drastically reduced. Accordingly, approximately 97%, 81%, and 71% of existing coal and conventional gas and oil resources, respectively, need to remain unburned. This article develops an integrated spatial assessment model based on estimates and locations of conventional oil resources and socio-environmental criteria to construct a global atlas of unburnable oil. The results show that biodiversity hotspots, richness centres of endemic species, natural protected areas, urban areas, and the territories of Indigenous Peoples in voluntary isolation coincide with 609 gigabarrels (Gbbl) of conventional oil resources. Since 1524 Gbbl of conventional oil resources are required to be left untapped in order to keep global warming under 1.5 °C, all of the above-mentioned socio-environmentally sensitive areas can be kept entirely off-limits to oil extraction. The model provides spatial guidelines to select unburnable fossil fuels resources while enhancing collateral socio-environmental benefits

First report of root-knot nematode Meloidogyne hispanica infecting grapevines in southern Spain

Artículo open access.Some commercial vineyards producing the ‘Condado de Huelva’ wine denomination of origin in Almonte, Bonares, and Rociana (Huelva Province), southern Spain, showed general decline in sandy soils in 2009. Disease surveys revealed severe infections of grapevine rootstock Richter 110 feeder roots and heavy soil infestations by a root-knot nematode (Meloidogyne sp.).Peer Reviewe

Description and molecular phylogeny of one new and one known needle nematode of the genus Paralongidorus (Nematoda: Longidoridae) from grapevine in Portugal using integrative approach.

A new and a known longidorid nematode, Paralongidorus lusitanicus n. sp. and Paralongidorus plesioepimikis, are described and illustrated from populations extracted from soil associated with grapevine (Vitis vinifera L.) from Escaroupim and Pó (central-Western Portugal), respectively. The new needle nematode P. lusitanicus n. sp. is characterised by a very large body size (8072–12,022 μm), an expanded and rounded lip region, ca 30 μm wide, with a clear constriction followed by a depression posterior to the amphidial aperture, amphidial fovea very large (11.0–19.0 μm), stirrupshaped, with conspicuous slit-like aperture as shown in scanning electron microscopy studies, a very long and flexible odontostyle (180.0–223.0 μm), guiding ring located at 28.0–41.5 μm from anterior end, vulva anterior to the mid-body (34–41%), a dorsally convex-conoid tail with rounded terminus (29–42 μm long), bearing two or three pairs of caudal pores and males common (ratio 1:1.6 females) with spicules ca 80 μm long. Morphological and morphometric traits for P. plesioepimikis fit well with the original description, and is reported for the first time in Portugal. Integrative diagnosis of both species was completed with molecular data obtained using D2-D3 expansion segments of 28S rDNA, ITS1-rDNA and partial 18S–rDNA. The phylogenetic relationships of these species with other Paralongidorus spp. using these three molecular markers indicated that P. lusitanicus n. sp. clustered together with other Paralongidorus spp. forming a sister clade with P. plesioepimikis, both of them sharing a large body, long odontostyle, an anteriorly located vulva and an expanded and rounded lip region with a clear constriction followed by a depression posterior to the amphidial apertur

Polyethylene glycol pulsed electrodeposition for the development of antifouling coatings on titanium

Titanium dental implants are widely used for the replacement of damaged teeth. However, bacterial infections at the interface between soft tissues and the implant can impair the functionality of the device and lead to failure. In this work, the preparation of an antifouling coating of polyethylene glycol (PEG) on titanium by pulsed electrodeposition was investigated in order to reduce Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) adhesion while maintaining human fibroblast adhesion. Different pulsed conditions were prepared and characterized by contact angle, Focused Ion Beam (FIB), Fourier Transformed Infrared Spectroscopy in the Attenuated Total Reflectance mode (ATR-FTIR), and X-ray photoelectron spectroscopy (XPS). XPS tested fibronectin adsorption. S. aureus, E. coli and human fibroblast adhesion was tested in vitro in both mono and co-culture settings. Physicochemical characterization proved useful for confirming the presence of PEG and evaluating the efficiency of the coating methods. Fibronectin adsorption decreased for all of the conditions, but an adsorption of 20% when compared to titanium was maintained, which supported fibroblast adhesion on the surfaces. In contrast, S. aureus and E. coli attachment on coated surfaces decreased up to 90% vs. control titanium. Co-culture studies with the two bacterial strains and human fibroblasts showed the efficacy of the coatings to allow for eukaryotic cell adhesion, even in the presence of pre-adhered bacteria.Peer ReviewedPostprint (published version