Geological record of tsunami inundations in Pantano Morghella (south-eastern Sicily) both from near and far-field sources

Abstract. Analysis of tsunami deposits from the Pantano Morghella area provided geological evidence for two inundations occurred along the south-eastern Ionian coast of Sicily. Pantano Morghella is a large pond characterised by a fine-grained sedimentation indicating a low-energy depositional environment. Two anomalous yellow sandy layers found at different depths indicate the occurrence of high-energy marine inundations. We studied sedimentological and paleontological features of the anomalous deposits as well as their spatial distribution observing the following properties: different facies with respect to the local stratigraphic sequence; erosive bases, rip-up clasts and broken elements testifying violent deposition mechanisms; macro and micro fauna of marine environment; relatively constant thickness throughout most of the depositional zone with thinning at the distal end; large sand sheets that extend inland. These observations, jointly with their infrequency in the sedimentary record and the age indicating a fast deposition, provided strong evidence for tsunami inundations. Correlations between anomalous layers and historical tsunamis are supported by radiocarbon and OSL dating results. The younger deposit is likely due to the 1908 near-source tsunami, whereas the flooding of the oldest event is most likely associated with a far and large source, the Crete 365 AD earthquake

Geological record of tsunami inundations in Pantano Morghella (south-eastern Sicily) both from near and far-field sources

Analysis of tsunami deposits from the Pantano Morghella area provided geological evidence for two inundations occurred along the south-eastern Ionian coast of Sicily. Pantano Morghella is a large pond characterised by a finegrained sedimentation indicating a low-energy depositional environment. Two anomalous yellow sandy layers found at different depths indicate the occurrence of high-energy marine inundations. We studied sedimentological and paleontological features of the anomalous deposits as well as their spatial distribution observing the following properties: different facies with respect to the local stratigraphic sequence; erosive bases, rip-up clasts and broken elements testifying violent deposition mechanisms; macro and micro fauna of marine environment; relatively constant thickness throughout most of the depositional zone with thinning at the distal end; large sand sheets that extend inland. These observations, jointly with their infrequency in the sedimentary record and the age indicating a fast deposition, provided strong evidence for tsunami inundations. Correlations between anomalous layers and historical tsunamis are supported by radiocarbon and OSL dating results. The younger deposit is likely due to the 1908 near-source tsunami, whereas the flooding of the oldest event is most likely associated with a far and large source, the Crete 365AD earthquake

Non-invasive Vagus Nerve Stimulation in Healthy Humans Reduces Sympathetic Nerve Activity

Background: Vagus nerve stimulation (VNS) is currently used to treat refractory epilepsy and is being investigated as a potential therapy for a range of conditions, including heart failure, tinnitus, obesity and Alzheimer's disease. However, the invasive nature and expense limits the use of VNS in patient populations and hinders the exploration of the mechanisms involved. Objective: We investigated a non-invasive method of VNS through electrical stimulation of the auricular branch of the vagus nerve distributed to the skin of the ear – transcutaneous VNS (tVNS) and measured the autonomic effects. Methods: The effects of tVNS parameters on autonomic function in 48 healthy participants were investigated using heart rate variability (HRV) and microneurography. tVNS was performed using a transcutaneous electrical nerve stimulation (TENS) machine and modified surface electrodes. Participants visited the laboratory once and received either active (200 μs, 30 Hz; n = 34) or sham (n = 14) stimulation. Results: Active tVNS significantly increased HRV in healthy participants (P = 0.026) indicating a shift in cardiac autonomic function toward parasympathetic predominance. Microneurographic recordings revealed a significant decrease in frequency (P = 0.0001) and incidence (P = 0.0002) of muscle sympathetic nerve activity during tVNS. Conclusion: tVNS can increase HRV and reduce sympathetic nerve outflow, which is desirable in conditions characterized by enhanced sympathetic nerve activity, such as heart failure. tVNS can therefore influence human physiology and provide a simple and inexpensive alternative to invasive VNS

Efficient Gene Replacements in Toxoplasma gondii Strains Deficient for Nonhomologous End Joining▿ †

A high frequency of nonhomologous recombination has hampered gene targeting approaches in the model apicomplexan parasite Toxoplasma gondii. To address whether the nonhomologous end-joining (NHEJ) DNA repair pathway could be disrupted in this obligate intracellular parasite, putative KU proteins were identified and a predicted KU80 gene was deleted. The efficiency of gene targeting via double-crossover homologous recombination at several genetic loci was found to be greater than 97% of the total transformants in KU80 knockouts. Gene replacement efficiency was markedly increased (300- to 400-fold) in KU80 knockouts compared to wild-type strains. Target DNA flanks of only ∼500 bp were found to be sufficient for efficient gene replacements in KU80 knockouts. KU80 knockouts stably retained a normal growth rate in vitro and the high virulence phenotype of type I strains but exhibited an increased sensitivity to double-strand DNA breaks induced by treatment with phleomycin or γ-irradiation. Collectively, these results revealed that a significant KU-dependent NHEJ DNA repair pathway is present in Toxoplasma gondii. Integration essentially occurs only at the homologous targeted sites in the KU80 knockout background, making this genetic background an efficient host for gene targeting to speed postgenome functional analysis and genetic dissection of parasite biology