Quantitative Storytelling: Science, Narratives, and Uncertainty in Nexus Innovations

Innovations are central instruments of sustainability policies. They project future visions onto technological solutions and enable win-win framings of complex sustainability issues. Yet, they also create new problems by interconnecting different resources such as water, food, and energy, what is known as the â WEF nexus.â In this paper, we apply a new approach called Quantitative Storytelling (QST) to the assessment of four innovations with a strong nexus component in EU policy: biofuels, shale gas, electric vehicles, and alternative water resources. Recognizing irreducible pluralism and uncertainties, QST inspects the relationships between the narratives used to frame sustainability issues and the evidence on those issues. Our experiences outlined two rationales for implementing QST. First, QST can be used to question dominant narratives that promote certain innovations despite evidence against their effectiveness. Second, QST can offer avenues for pluralistic processes of co-creation of alternative narratives and imaginaries. We reflect on the implementation of QST and on the role played by different uncertainties throughout these processes. Our experiences suggest that while the role of nexus assessments using both numbers and narratives may not be instrumental in directly inducing policy change, they are valuable means to open discussions on innovations outside of dominant nexus imaginaries. © The Author(s) 2021.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research has been funded by the European Union’s H2020 project MAGIC: Moving towards Adaptive Governance in Complexity (MAGIC GA No. 689669); European Union’s FP7 project IANEX: Integrated Assessment of the Nexus: The Case of Hydraulic Fracturing, Marie Curie International Outgoing Fellowship GA No. 623593; and Spanish Ministry of Science’s Juan de la Cierva Fellowship (IJC2019-038847-I/AEI/10.13039/501100011033)

Influence of topography on tide propagation and amplification in semi-enclosed basins

An idealized model for tide propagation and amplification in semi-enclosed rectangular basins is presented, accounting for depth differences by a combination of longitudinal and lateral topographic steps. The basin geometry is formed by several adjacent compartments of identical width, each having either a uniform depth or two depths separated by a transverse topographic step. The problem is forced by an incoming Kelvin wave at the open end, while allowing waves to radiate outward. The solution in each compartment is written as the superposition of (semi)-analytical wave solutions in an infinite channel, individually satisfying the depth-averaged linear shallow water equations on the f plane, including bottom friction. A collocation technique is employed to satisfy continuity of elevation and flux across the longitudinal topographic steps between the compartments. The model results show that the tidal wave in shallow parts displays slower propagation, enhanced dissipation and amplified amplitudes. This reveals a resonance mechanism, occurring when\ud the length of the shallow end is roughly an odd multiple of the quarter Kelvin wavelength. Alternatively, for sufficiently wide basins, also Poincaré waves may become resonant. A transverse step implies different wavelengths of the incoming and reflected Kelvin wave, leading to increased amplitudes in shallow regions and a shift of amphidromic points in the direction of the deeper part. Including the shallow parts near the basin’s closed end (thus capturing the Kelvin resonance mechanism) is essential to reproduce semi-diurnal and diurnal\ud tide observations in the Gulf of California, the Adriatic Sea and the Persian Gulf

The Spectrum of Electromagnetic Jets from Kerr Black Holes and Naked Singularities in the Teukolsky Perturbation Theory

We give a new theoretical basis for examination of the presence of the Kerr black hole (KBH) or the Kerr naked singularity (KNS) in the central engine of different astrophysical objects around which astrophysical jets are typically formed: X-ray binary systems, gamma ray bursts (GRBs), active galactic nuclei (AGN), etc. Our method is based on the study of the exact solutions of the Teukolsky master equation for electromagnetic perturbations of the Kerr metric. By imposing original boundary conditions on the solutions so that they describe a collimated electromagnetic outflow, we obtain the spectra of possible {\em primary jets} of radiation, introduced here for the first time. The theoretical spectra of primary electromagnetic jets are calculated numerically. Our main result is a detailed description of the qualitative change of the behavior of primary electromagnetic jet frequencies under the transition from the KBH to the KNS, considered here as a bifurcation of the Kerr metric. We show that quite surprisingly the novel spectra describe linearly stable primary electromagnetic jets from both the KBH and the KNS. Numerical investigation of the dependence of these primary jet spectra on the rotation of the Kerr metric is presented and discussed.Comment: 18 pages, 35 figures, LaTeX file. Final version. Accepted for publication in Astrophysics and Space Science. Amendments. Typos corrected. Novel notion -"primary jet" is introduced. New references and comments adde

Extended Emission from Short Gamma-Ray Bursts Detected with SPI-ACS/INTEGRAL

The short duration (T90 < 2 s) gamma-ray bursts (GRBs) detected in the SPI-ACS experiment onboard the INTEGRAL observatory are investigated. Averaged light curves have been constructed for various groups of events, including short GRBs and unidentified short events. Extended emission has been found in the averaged light curves of both short GRBs and unidentified short events. It is shown that the fraction of the short GRBs in the total number of SPI-ACS GRBs can range from 30 to 45%, which is considerably larger than has been thought previously.Comment: 27 pages, 10 figure

Genomic insertion of a heterologous acetyltransferase generates a new lipopolysaccharide antigenic structure in brucella abortus and brucella melitensis

Brucellosis is a bacterial zoonosis of worldwide distribution caused by bacteria of the genus Brucella. In Brucella abortus and Brucella melitensis, the major species infecting domestic ruminants, the smooth lipopolysaccharide (S-LPS) is a virulence factor. This S-LPS carries a N-formyl-perosamine homopolymer O-polysaccharide that is the major antigen in serodiagnostic tests and is required for virulence. We report that the Brucella O-PS can be structurally and antigenically modified using wbdR, the acetyl-transferase gene involved in N-acetyl-perosamine synthesis in Escherichia coli O157:H7. Brucella constructs carrying plasmidic wbdR expressed a modified O-polysaccharide but were unstable, a problem circumvented by inserting wbdR into a neutral site of chromosome II. As compared to wild-type bacteria, both kinds of wbdR constructs expressed shorter O-polysaccharides and NMR analyses showed that they contained both N-formyl and N-acetyl-perosamine. Moreover, deletion of the Brucella formyltransferase gene wbkC in wbdR constructs generated bacteria producing only N-acetyl-perosamine homopolymers, proving that wbdR can replace for wbkC. Absorption experiments with immune sera revealed that the wbdR constructs triggered antibodies to new immunogenic epitope(s) and the use of monoclonal antibodies proved that B. abortus and B. melitensis wbdR constructs respectively lacked the A or M epitopes, and the absence of the C epitope in both backgrounds. The wbdR constructs showed resistance to polycations similar to that of the wild-type strains but displayed increased sensitivity to normal serum similar to that of a per R mutant. In mice, the wbdR constructs produced chronic infections and triggered antibody responses that can be differentiated from those evoked by the wild-type strain in S-LPS ELISAs. These results open the possibilities of developing brucellosis vaccines that are both antigenically tagged and lack the diagnostic epitopes of virulent field strains, thereby solving the diagnostic interference created by current vaccines against Brucella

Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health.

The short-lived turquoise killifish Nothobranchius furzeri (Nfu) is a valid model for aging studies. Here, we investigated its age-associated cardiac function. We observed oxidative stress accumulation and an engagement of microRNAs (miRNAs) in the aging heart. MiRNA-sequencing of 5 week (young), 12-21 week (adult) and 28-40 week (old) Nfu hearts revealed 23 up-regulated and 18 down-regulated miRNAs with age. MiR-29 family turned out as one of the most up-regulated miRNAs during aging. MiR-29 family increase induces a decrease of known targets like collagens and DNA methyl transferases (DNMTs) paralleled by 5´methyl-cytosine (5mC) level decrease. To further investigate miR-29 family role in the fish heart we generated a transgenic zebrafish model where miR-29 was knocked-down. In this model we found significant morphological and functional cardiac alterations and an impairment of oxygen dependent pathways by transcriptome analysis leading to hypoxic marker up-regulation. To get insights the possible hypoxic regulation of miR-29 family, we exposed human cardiac fibroblasts to 1% O &lt;sub&gt;2&lt;/sub&gt; levels. In hypoxic condition we found miR-29 down-modulation responsible for the accumulation of collagens and 5mC. Overall, our data suggest that miR-29 family up-regulation might represent an endogenous mechanism aimed at ameliorating the age-dependent cardiac damage leading to hypertrophy and fibrosis