Sistem Informasi Pemesanan Furniture Berbahan Baku Aluminium Pada Usaha Dagang Crystal Aluminium Manokwari Berbasis WEB

Sistem informasi pemesanan furniture berbahan baku alumunium  berbasis web pada usaha dagang crystal aluminum” diharapkan akan dapat memberikan gambaran informasi dan transaksi pemesanan furniture berbahan almunium. Adapun Penelitian ini bertujuan untuk : (1) Merancang sebuah sistem informasi pemesanan furniture berbahan baku almunium berbasis web pada usaha dagang crystal aluminium (2) Mengimplementasikan sebuah sistem informasi pemesanan furniture berbahan baku almunium berbasis web pada usaha dagang crystal aluminum. Metode penelitian ini dilakukan dengan menggunakan metode interview, observasi dan dokumentasi. Perancangan sistem dilakukan dengan bahasa pemodelan dengan menggunakan UML(Unified Modelling Language) yang terdiri dari use case diagram, activity diagram dan sequence diagram. Sedangkan pemograman yang dipakai adalah HyperText Markup Language(HTML), Cascading Style Sheets(CSS), Hypertext Preprocessor(PHP), JQUERY, Content Management System(CMS) yang terdiri dari Wordpress dan Woocommerce dengan menggunakan database MySQL. Melakukan pengujian program dengan menggunakan metode blackbox sebagai tahap akhir dalam pembuatan aplikasi ini, dengan hasil pengujian yang bebas dari kesalahan logika. Hasil ini menunjukkan bahwa dengan adanya sistem informasi pemesanan furniture berbahan baku almunium berbasis web pada usaha dagang crystal aluminum dapat mempermudah Pemesanan dalam pemesan furniture almunium dan member untuk melihat  selama ini sehingga Admin (Karyawan) dalam mengolah pemesanan dan melihat laporan transaksi. Adapun fitur yang tampil di website yaitu beranda, kategori, requist, akun saya, kotak saran dan tentan

Plasma Membrane Polarity and Compartmentalization Are Established before Cellularization in the Fly Embryo

SummaryPatterning in the Drosophila embryo requires local activation and dynamics of proteins in the plasma membrane (PM). We used in vivo fluorescence imaging to characterize the organization and diffusional properties of the PM in the early embryonic syncytium. Before cellularization, the PM is polarized into discrete domains having epithelial-like characteristics. One domain resides above individual nuclei and has apical-like characteristics, while the other domain is lateral to nuclei and contains markers associated with basolateral membranes and junctions. Pulse-chase photoconversion experiments show that molecules can diffuse within each domain but do not exchange between PM regions above adjacent nuclei. Drug-induced F-actin depolymerization disrupted both the apicobasal-like polarity and the diffusion barriers within the syncytial PM. These events correlated with perturbations in the spatial pattern of dorsoventral Toll signaling. We propose that epithelial-like properties and an intact F-actin network compartmentalize the PM and shape morphogen gradients in the syncytial embryo

Sistem Diagnosa Penyakit Jantung Dengan Metode Fuzzy Inference Sistem

Sistem Pakar merupakan suatu sistem komputer yang menyamai kemampuan pengambilan keputusan dari seorang pakar. Kata menyamai tersebut memiliki pengertian bahwa Sistem Pakar diharapkan dapat bekerja dalam semua hal seperti halnya seorang pakar. Penelitian ini ditujukan untuk mengimplementasi algoritna Fuzzy Inference System pada Sistem pakar untuk mendiagnosa penyakit jantung dengan tujuan membantu para pakar penyakit jantung dalam mendiagnosa tingkat penyakit pasien, khususnya penderita sakit jantung. Didasari dari gejala-gejala awal yang terjadi pada pasien penderita sakit jantung yang kemudian diproses oleh system dan menghasilkan output diagnosa penyakit pasien tersebut, yang diharapkan akurat dan layak digunakan untuk mendiagnosa medis. Dengan batasan difokuskan pada implementasi Fuzzy Inference System dalam mendiagnosa penyakit jantung dan tidak membahas algoritmanya

Genetic Analysis in Drosophila Reveals a Role for the Mitochondrial Protein P32 in Synaptic Transmission

Mitochondria located within neuronal presynaptic terminals have been shown to play important roles in the release of chemical neurotransmitters. In the present study, a genetic screen for synaptic transmission mutants of Drosophila has identified the first mutation in a Drosophila homolog of the mitochondrial protein P32. Although P32 is highly conserved and has been studied extensively, its physiological role in mitochondria remains unknown and it has not previously been implicated in neural function. The Drosophila P32 mutant, referred to as dp32EC1, exhibited a temperature-sensitive (TS) paralytic behavioral phenotype. Moreover, electrophysiological analysis at adult neuromuscular synapses revealed a TS reduction in the amplitude of excitatory postsynaptic currents (EPSC) and indicated that dP32 functions in neurotransmitter release. These studies are the first to address P32 function in Drosophila and expand our knowledge of mitochondrial proteins contributing to synaptic transmission

Visualizing septins in early Drosophila embryos

International audienceFunctional studies in Drosophila have been key for establishing a role for the septin family of proteins in animal cell division and thus extending for the first time observations from the budding yeast to animal cells. Visualizing the distribution of specific septins in different Drosophila tissues and, in particular, in the Drosophila embryo, together with biochemical and mutant phenotype data, has contributed important advances to our understanding of animal septin biology, suggesting roles in processes other than in cytokinesis. Septin localization using immunofluorescence assays has been possible due to the generation of antibodies against different Drosophila septins. The recent availability of lines expressing fluorescent protein fusions of specific septins further promises to facilitate studies on septin dynamics. Here, we provide protocols for preparing early Drosophila embryos to visualize septins using immunofluorescence assays and live fluorescence microscopy. The genetic tractability of the Drosophila embryo together with its amenability to high-resolution fluorescence microscopy promises to provide novel insights into animal septin structure and function

The cystic fibrosis transmembrane recruiter the alter ego of CFTR as a multi-kinase anchor

This review focuses on a newly discovered interaction between protein kinases involved in cellular energetics, a process that may be disturbed in cystic fibrosis for unknown reasons. I propose a new model where kinase-mediated cellular transmission of energy provides mechanistic insight to a latent role of the cystic fibrosis transmembrane conductance regulator (CFTR). I suggest that CFTR acts as a multi-kinase recruiter to the apical epithelial membrane. My group finds that, in the cytosol, two protein kinases involved in cell energy homeostasis, nucleoside diphosphate kinase (NDPK) and AMP-activated kinase (AMPK), bind one another. Preliminary data suggest that both can also bind CFTR (function unclear). The disrupted role of this CFTR-kinase complex as ‘membrane transmitter to the cell’ is proposed as an alternative paradigm to the conventional ion transport mediated and CFTR/chloride-centric view of cystic fibrosis pathogenesis. Chloride remains important, but instead, chloride-induced control of the phosphohistidine content of one kinase component (NDPK, via a multi-kinase complex that also includes a third kinase, CK2; formerly casein kinase 2). I suggest that this complex provides the necessary near-equilibrium conditions needed for efficient transmission of phosphate energy to proteins controlling cellular energetics. Crucially, a new role for CFTR as a kinase controller is proposed with ionic concentration acting as a signal. The model posits a regulatory control relay for energy sensing involving a cascade of protein kinases bound to CFTR

Syndapin bridges the membrane-cytoskeleton divide during furrow extension

BAR domain proteins can regulate ‘membrane reservoirs’ that provide surface area and buffer membrane tension. Syndapin is an F-BAR and SH3 domain containing protein involved in cytoskeletal remodelling and endocytosis. The Syndapin F-BAR domain is uniquely versatile compared to others in the family and can bend phospholipid membranes into tubules of various diameters and directly bind actin. The Syndapin SH3 domain can also interact with actin remodelling proteins and modulate cytoskeletal contractility. Pseudocleavage furrow extension in the syncytial division cycles of Drosophila embryos requires the homeostatic control of conserved processes that control plasma membrane tension and actin contractility. We find that Syndapin plays an important role in promoting pseudocleavage furrow extension. We propose a model involving roles for Syndapin in membrane dynamics and direct or indirect effect on the cytoskeleton to explain how it affects pseudocleavage furrow growth, independent of its role in endocytosis