A Novel Antigen Sensitive to Calcium Chelation That is Associated with the Tip Links and Kinocilial Links of Sensory Hair Bundles

Tip links are extracellular, cell-surface-associated filaments of unknown molecular composition that are thought to gate the mechanotransducer channel of the sensory hair cell. They disappear from the hair bundle in response to calcium chelation and lanthanum treatment and resist degradation by the protease subtilisin. A monoclonal antibody derived from a hybridoma screen identified a novel antigen associated with tip links, the tip-link antigen. The tip-link antigen is also associated with kinocilial links, subtilisin-resistant filaments that are sensitive to calcium chelation and connect the kinocilium to the tallest stereocilia of the hair bundle. Furthermore, the tip-link antigen is expressed in the retina, where it is associated with the ciliary calyx, a ring of microvilli that surrounds the outer segment of the photoreceptor. The tip-link antigen rapidly disappears from the surface of the hair bundle in response to calcium chelation. It is also subtilisin resistant, relative to the ankle-link antigen, an antigen associated with another type of hair bundle link. The tip-link antigen is lanthanum sensitive and, like tip links, reappears on the surface of the hair bundle after calcium chelation. The monoclonal antibody to the tip-link antigen immunoprecipitates two concanavalin A-reactive polypeptides with apparent molecular masses of 200 and 250 kDa from detergent extracts of the retina. These results provide the first identification of a cell surface antigen associated with tip links, indicate that tip links share properties in common with kinocilial links, and reveal a second epitope that, along with the ankle-link antigen, is common to both sensory hair bundles and the ciliary calyx of photoreceptors

CO J = 3→2 and J = 2→1 mapping and spectroscopy of NGC 7027

We present spectra and mapping for NGC 7072 in the J = 3→2 and J = 2→1 transitions of CO. The central profile at J = 2→1 is shown to be very similar to the J = 1→0 spectrum measured by Thronson (1983), and this implies a source expansion at roughly constant velocity. The J = 3→2 line however appears weaker, with evidence for appreciable quenching of the higher velocity components. Detailed modelling f the source indicates that densities n must vary appreciably with shell radius R(as nα R-a, where α≥2), and this leads to a corresponding steep radial decrease in the radiation temperature TR. In consequence, the source FWHM is found to decrease appreciably iwth increasing transition frequency, a trend which appears also to be confirmed by our central J = 3→2 scans. It is not however possible to constrain gas kinetic tempertures TK, the level of CO thermalisation, or shell mass M with any degree of confidence - both low and high mass models appear capable of replicating our spectra. Finally, the J = 2→1 spatial velocity map displays evidence for a decrease in velocity width towards the outer regions of the nebula; a feature which is expected of most outflow models. The J = 3→2 map also indicates the presence of a nebular extension to the north-west of the peak emission core, although this is not reproduced in the corresponding J = 1→0 map of Mufson et al. (1975)

Asymptotic solution of a model for bilayer organic diodes and solar cells

The current voltage characteristics of an organic semiconductor diode made by placing together two materials with dissimilar electron affinities and ionisation potentials is analysed using asymptotic methods. An intricate boundary layer structure is examined. We find that there are three regimes for the total current passing through the diode. For reverse bias and moderate forward bias the dependency of the voltage on the current is similar to the behaviour of conventional inorganic semiconductor diodes predicted by the Shockley equation and are governed by recombination at the interface of the materials. There is then a narrow range of currents where the behaviour undergoes a transition. Finally for large forward bias the behaviour is different with the current being linear in voltage and is primarily controlled by drift of charges in the organic layers. The size of the interfacial recombination rate is critical in determining the small range of current where there is rapid transition between the two main regimes. The extension of the theory to organic solar cells is discussed and the analogous current voltage curves derived in the regime of interest

Novel Fiber Design for Wideband Conversion and Amplification in Multimode Fibers

We propose an operating principle to achieve broadband and highly tunable mode conversion and amplification exploiting inter-modal four wave mixing in a multimode fiber. A bandwidth of 30 nanometers is demonstrated by properly designing a simple step-index silica fiber.Comment: Ecoc conference 201

Near infrared spectroscopy of W51 IRS-2

Near-infrared spectra at 2.95-3.5 μm and 3.99-10 μm have been obtained towards W51 IRS-2 and its surroundings, in order to investigate the spatial variations in intensity of the 3.28 μm unidentified feature and the 4.05 μm Brackett-α line. The Br-α and 3.28 μm features occupy a broadly similar spatial zone, which is characterised by an unresolved core responsible for most of the emission, and an extended and considerably weaker halo. Grain properties required to excite the 4.28 microns line, the nature of the 3.28 μm emission, and its relation to the source structure are discussed

Community Structure in Time-Dependent, Multiscale, and Multiplex Networks

Network science is an interdisciplinary endeavor, with methods and applications drawn from across the natural, social, and information sciences. A prominent problem in network science is the algorithmic detection of tightly-connected groups of nodes known as communities. We developed a generalized framework of network quality functions that allowed us to study the community structure of arbitrary multislice networks, which are combinations of individual networks coupled through links that connect each node in one network slice to itself in other slices. This framework allows one to study community structure in a very general setting encompassing networks that evolve over time, have multiple types of links (multiplexity), and have multiple scales.Comment: 31 pages, 3 figures, 1 table. Includes main text and supporting material. This is the accepted version of the manuscript (the definitive version appeared in Science), with typographical corrections included her

Tungsten thermal neutron dosimeter

Tungsten-185 activity, which is produced by neutron activation of tungsten-184, determines thermal neutron flux. Radiochemical separation methods and counting techniques for irradiated tungsten provide accurate determination of the radiation exposure

Nonlinear optical thresholding in a 4-Channel OCDMA system via two-photon absorption

We demonstrate the use of a Two-Photon Absorption based detector in an OCDMA system. This detector provides a significant performance improvement over standard linear detection

Integrating multiple representations: fighting asthma

This paper seeks to engage debates about integrating pluralisms regarding multiple forms/representations and how they might function smoothly if they are closely aligned. This paper offers, narrative poetry with an artistic impression aimed at seeing how these might interact with each other. Like poetry, visual images are unique and can evoke particular kinds of emotional and visceral responses. By offering narrative poetry together with an artistic representation it is not meant to de-value the importance of either, but it is aimed at seeing how these arts-based methods and creative analytical practices might unite as a narrative to offer knew ways of ‘knowing’ and ‘seeing