Towards the characterisation of automotive specific woven composite laminates at intermediate strain rates

This thesis examines the sensitivity of an automotive specific T700 12K 2 x 2 twill carbon fibre epoxy composite to intermediate strain rate loads. Literature to date has focused on characterising uni-directional laminates at intermediate strain rates with few individual studies on aerospace 1K and 3K woven fabrics, however, there is a dearth of information on the strain rate effect of large tow woven fabrics at intermediate strain rates. Preliminary ASTM standard quasi-static experiments highlighted the heterogeneity of the surface strains in both tension and in-plane shear through the application of 3D digital image correlation full field measurements. Experimental studies in literature conventionally use small specimens to induce elevated strain rates however, this thesis demonstrates that due to the heterogeneous strain field, small specimens induce erroneous failure mechanisms in a material with such a large reciprocating tow structure. To overcome this, a new specimen sizing methodology was employed based on determining the representative surface element of the specimen through quadrangular windowing of the measured surface strains. Experimental correlation showed no statistical difference in comparison to the ASTM standard results. Since the specimens induced failure loads of ~ 50 kN, a new high capacity slack adaptor was designed and commissioned as composite materials with this failure load had previously not been explored at intermediate strain rates. The experimental response of the representative tensile and shear specimens was investigated at discrete intervals between nominal longitudinal strain rates of 2.2 x 10-4 s-1 and 1.0 x 102 s-1. Surface strain analysis at all strain rates using 3D DIC enabled review of the damage mechanisms occurring over the specimen. Woven tensile specimens were shown to be more sensitive to strain rate than UD laminates with T700 carbon fibres that have previously investigated in literature with large increases in strength and modulus observed. This is thought to be a result of the large resin rich regions created by the interstitial sites of the 400 gsm - 12K fabric and the large elliptical tow boundaries. Ultimate tensile strain was also shown to increase significantly at low strain rates prior to stabilising, this was hypothesised to be the due to the rate effect of damage coalescence whilst the fibres remain insensitive to strain rate. Shear specimens showed statistically significant increases in modulus, yield shear strain, yield shear strength and ultimate shear strength whilst ultimate shear strain was shown to be truncated with increasing strain rate. It was shown that the currently available finite element material cards for modelling strain rate sensitivity within LS-DYNA lack accuracy to model the strain rate effect of longitudinal tensile, shear and quasi-isotropic specimens. The modelled tensile response was shown to be more ductile than the experiment at fracture. This inaccuracy was compounded when attempting to model the tensile strain rate sensitivity due to the inability replicate the stiffness increase with rate. In comparison shear modelling was capable of predicting the bi-linear response with rate, however, it was unable to terminate the element, inducing high inaccurate virtual strain energies. This thesis highlights the critical importance of strain rate modelling of automotive specific woven composite materials for CAE vehicle development through extensive experimental studies, and it recommends that the current material cards and appropriate phenomenological models require further research and development

On the static and dynamic properties of flax and Cordenka epoxy composites

Fibre reinforced composites have excellent specific properties and are widely sought after by engineers seeking to reduce mass. However, end of life disposal is a significant problem and so research into more sustainable natural fibre composites is extremely topical. This paper examines the applicability of natural fibre composites for high performance structural applications. Woven flax and regenerated cellulose (Cordenka) textiles were pre-impregnated with commercially available epoxy resins and consolidated into test laminates in an autoclave to determine their static (compressive, tensile, flexural) and dynamic (energy absorption) properties. The range of compressive strengths was 77.5–299.6 MPa. Tensile strengths ranged from 63 to 92.6 MPa and interlaminar shear strength (ILSS) from 10.7 to 23.3 MPa. Specific energy absorption (SEA) varied between 21.2–34.2 kJ/kg. Biotex flax combined with MTM49 resin matched the SEA of T300 carbon fibre using the same resin system and layup. This work has demonstrated that natural fibre composites have significant scope for use in structural applications but additional work is required on fibre to matrix bonding in order to maximise their properties whilst remaining an environmentally credible option

A Performance Versus Cost Analysis of Prepreg Carbon Fibre Epoxy Energy Absorption Structures

Carbon fibre epoxy composites are sought after for their excellent specific energy absorption (SEA) but are costly. A range of prepreg carbon fibre epoxy layups were subjected to a 10 m/s impact with 4 kJ of energy. Fibre volume fraction and voidage were determined for each sample and the fracture analysed in detail. SEA ranged from 35.27 J/g to 60.25 J/g with the highest performance from 8 plies of 200gsm 2x2 twill all laid at 0 degrees. Vacuum assisted oven cure resulted in higher voidage than autoclave cure (2.52% versus 0.17%) but did not affect SEA. According to a ratio of performance to cost the highest rated samples were an 8 ply oven cure and a 3 ply autoclave cure specimen and there was little difference between them. This work has highlighted that there is enormous potential for cost reduction of prepreg carbon fibre epoxy energy absorption structures through the use of heavier areal weight fabrics and fewer plies as well as through the use of oven cured prepreg

Blood pressure self-monitoring in pregnancy: examining feasibility in a prospective cohort study

Background: Raised blood pressure (BP) affects approximately 10% of pregnancies worldwide, and a high proportion of affected women develop pre-eclampsia. This study aimed to evaluate the feasibility of self-monitoring of BP in pregnancy in women at higher risk of pre-eclampsia. Methods: This prospective cohort study of self-monitoring BP in pregnancy was carried out in two hospital trusts in Birmingham and Oxford and thirteen primary care practices in Oxfordshire. Eligible women were those defined by the UK National Institute for Health and Care Excellence (NICE) guidelines as at higher risk of pre-eclampsia. A total of 201 participants were recruited between 12 and 16 weeks of pregnancy and were asked to take two BP readings twice daily three times a week through their pregnancy. Primary outcomes were recruitment, retention and persistence of self-monitoring. Study recruitment and retention were analysed with descriptive statistics. Survival analysis was used to evaluate the persistence of self-monitoring and the performance of self-monitoring in the early detection of gestational hypertension, compared to clinic BP monitoring. Secondary outcomes were the mean clinic and self-monitored BP readings and the performance of self-monitoring in the detection of gestational hypertension and pre-eclampsia compared to clinic BP. Results: Of 201 women recruited, 161 (80%) remained in the study at 36 weeks or to the end of their pregnancy, 162 (81%) provided any home readings suitable for analysis, 148 (74%) continued to self-monitor at 20 weeks and 107 (66%) at 36 weeks. Self-monitored readings were similar in value to contemporaneous matched clinic readings for both systolic and diastolic BP. Of the 23 who developed gestational hypertension or pre-eclampsia and self-monitored, 9(39%) had a raised home BP prior to a raised clinic BP. Conclusions: Self-monitoring of BP in pregnancy is feasible and has potential to be useful in the early detection of gestational hypertensive disorders but maintaining self-monitoring throughout pregnancy requires support and probably enhanced training

KI67 and DLX2 predict increased risk of metastasis formation in prostate cancer - a targeted molecular approach

Background: There remains a need to identify and validate biomarkers for predicting prostate cancer (CaP) outcomes using robust and routinely available pathology techniques to identify men at most risk of premature death due to prostate cancer. Previous immunohistochemical studies suggest the proliferation marker Ki67 might be a predictor of survival, independently of PSA and Gleason score. We performed a validation study of Ki67 as a marker of survival and disease progression and compared its performance against another candidate biomarker, DLX2, selected using artificial neural network analysis. Methods: A tissue microarray (TMA) was constructed from transurethral resected prostatectomy histology samples (n=192). Artificial neural network analysis was used to identify candidate markers conferring increased risk of death and metastasis in a public cDNA array. Immunohistochemical analysis of the TMA was carried out and univariate and multivariate tests performed to explore the association of tumour protein levels of Ki67 and DLX2 with time to death and metastasis. Results: Univariate analysis demonstrated Ki67 as predictive of CaP-specific survival (DSS; P=0.022), and both Ki67 (P=0.025) and DLX2 (P=0.001) as predictive of future metastases. Multivariate analysis demonstrated Ki67 as independent of PSA, Gleason score and D’Amico risk category for DSS (HR=2.436, P=0.029) and both Ki67 (HR=3.296, P=0.023) and DLX2 (HR=3.051, P=0.003) as independent for future metastases. Conclusions: High Ki67 expression is only present in 6.8% of CaP patients and is predictive of reduced survival and increased risk of metastasis, independent of PSA, Gleason score and D’Amico risk category. DLX2 is a novel marker of increased metastasis risk found in 73% patients and 8.2% showed co-expression with a high Ki67 score. Two cancer cell proliferation markers, Ki67 and DLX2, may be able to inform clinical decision-making when identifying patients for active surveillance

Synthesis and characterization of high-affinity 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled fluorescent ligands for human β-adrenoceptors

The growing practice of exploiting noninvasive fluorescence-based techniques to study G protein-coupled receptor pharmacology at the single cell and single molecule level demands the availability of high-quality fluorescent ligands. To this end, this study evaluated a new series of red-emitting ligands for the human β-adrenoceptor family. Upon the basis of the orthosteric ligands propranolol, alprenolol, and pindolol, the synthesized linker-modified congeners were coupled to the commercially available fluorophore BODIPY 630/650-X. This yielded high-affinity β-adrenoceptor fluorescent ligands for both the propranolol and alprenolol derivatives; however, the pindolol-based products displayed lower affinity. A fluorescent diethylene glycol linked propranolol derivative (18a) had the highest affinity (log KD of -9.53 and -8.46 as an antagonist of functional β2- and β1-mediated responses, respectively). Imaging studies with this compound further confirmed that it can be employed to selectively label the human β2-adrenoceptor in single living cells, with receptor-associated binding prevented by preincubation with the nonfluorescent β2-selective antagonist 3-(isopropylamino)-1-[(7-methyl-4-indanyl)oxy]-butan-2-ol (ICI 118551) (J. Cardiovasc. Pharmacol. 1983, 5, 430-437.

The mediation of social influences on smoking cessation and awareness of the early signs of lung cancer

Background Whilst there has been no clear consensus on the potential for earlier diagnosis of lung cancer, recent research has suggested that the time between symptom onset and consultation can be long enough to plausibly affect prognosis. In this article, we present findings from a qualitative study involving in-depth interviews with patients who had been diagnosed with lung cancer (n = 11), and people who were at heightened risk of developing the disease (n = 14). Methods A grounded theory methodology was drawn upon to conduct thematic and narrative based approaches to analysis. Results The paper focuses on three main themes which emerged from the study: i) fatalism and resignation in pathways to help-seeking and the process of diagnosis; ii) Awareness of smoking risk and response to cessation information and advice. iii) The role of social and other networks on help-seeking. Key findings included: poor awareness among participants of the symptoms of lung cancer; ambivalence about the dangers of smoking; the perception of lung cancer as part of a homogenisation of multiple illnesses; close social networks as a key trigger in help-seeking. Conclusions We suggest that future smoking cessation and lung cancer awareness campaigns could usefully capitalise on the influence of close social networks, and would benefit from taking a ‘softer’ approach