Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection

BACKGROUND: A polymerase chain reaction (PCR)-based method for quantitating CD4 and CD8 mRNA could provide a means of assessing immune status of AIDS patients and other immunologically compromised persons without requiring large blood draws, and could be exquisitely sensitive. Such a method would also be useful in assessing the immune status of patients retrospectively. RESULTS: Quantitative competitive reverse transcription PCR (QC-RT-PCR) assays were developed for measurement of CD4 and CD8 mRNA. Samples were obtained from HIV-positive and negative patients whose CD4 and CD8 counts had been determined via Flow Cytometry. The quantity of CD4 (n = 13) and CD8 (n = 28) mRNA standardized according to GAPDH mRNA quantities, all determined by QC-RT-PCR, were compared to cell number as determined by flow cytometry. There was no correlation between CD4 and CD8 cell counts and mRNA levels of CD4 and CD8 as determined by QC-RT-PCR. There is no correlation between CD4 and CD8 mRNA levels and the number of cells expressing these proteins on their surface. CONCLUSION: QC-RT-PCR, and related methodologies are not useful substitutes for assessment of CD4 and CD8 cell numbers in HIV-infected persons

1972: Abilene Christian College Bible Lectures - Full Text

THE CHURCH AND THE FUTURE Being the Abilene Christian College Annual Bible Lectures 1972 Published by ABILENE CHRISTIAN COLLEGE BOOK STORE ACC Station Abilene, Texas 7960

Commercial clam production : annual report

"Annual report : October 1, 1980 to September 30, 1981" "During the year we continued our studies on the clam resources in Oregon's estuaries. Our objectives were: to collect data for developing a scientifically sound clam management database and to refine techniques for assessing the potential for a commercial clam fishery in several of Oregon's estuaries. In addition we continued to monitor the growth of laboratory reared Manila littleneck clams (Tapes japonica) released in Netarts Bay." (from the Introduction

Commercial clam production

"Completion report : October 1, 1979 to December 31, 1981" "We completed Our assessment of commercial clam harvest potential in Tillamook, Yaquina and Coos bays. All three bays contained commercial quantities of clams. Detailed studies in Yaquina Bay in 1981 revealed a biomass estimate of 2.6 million pounds (1,179.1 mt) of gaper clams (Tresus capax) in the study tract." (from the Abstract