Proof of Grünbaum's conjecture on the stretchability of certain arrangements of pseudolines

AbstractWe prove Grünbaum's conjecture that every arrangement of eight pseudolines in the projective plane is stretchable, i.e., determines a cell complex isomorphic to one determined by an arrangement of lines. The proof uses our previous results on ordered duality in the projective plane and on periodic sequences of permutations of [1,n] associated to arrangements of n lines in the euclidean plane

On the combinatorial classification of nondegenerate configurations in the plane

AbstractWe classify nondegenerate plane configurations by attaching, to each such configuration of n points, a periodic sequence of permutations of {1, 2, …, n} which satisfies some simple conditions; this classification turns out to be appropriate for questions involving convexity. In 1881 Perrin stated that every sequence satisfying these conditions was the image of some plane configuration. We show that this statement is incorrect by exhibiting a counterexample, for n = 5, and prove that for n ⩽ 5 every sequence essentially distinct from this one is realized geometrically by giving a complete classification of configurations in these cases; there is 1 combinatorial equivalence class for n = 3, 2 for n = 4, and 19 for n = 5. We develop some basic notions of the geometry of “allowable sequences” in the course of proving this classification theorem. Finally, we state some results and an open problem on the realizability question in the general case

Piloting Participatory Arts-Based Methods for Exploring Indonesians’ Experiences in a U.S. Biotechnology Training Program

Science communication faculty and professionals often train scientists about conveying and delivering critical and sometimes controversial scientific information to public audiences. This qualitative case study was situated in a U.S.-based biotechnology training program funded by the United States Department of Agriculture for connecting Indonesian science fellows with university biotechnology scientists and science communication experts. The researchers piloted a participatory arts-based approach for instructing and researching Indonesian scientists’, professionals’, and educators’ learning and experiences in the program. Participatory and arts-based research has the potential to uncover and bring to light participants’ perceptions. Participants used iPad multimedia kits to demonstrate their learning of the training’s science communication content, co-constructed and conducted interviews, and captured photos and videos chronicling their experiences throughout the program. Results showed participants’ photos predominantly focused on field site and laboratory visits during the training and participants effectively applied digital storytelling techniques presented in the workshops. Themes from the co-constructed interviews included participants’ definitions of biotechnology, concerns about regulation, labeling, and public understanding, and expressing a hope that biotechnology may improve food security in Indonesia. This pilot study has implications for future international science communication training via intentional instructional design and arts-based research for a culture-centered communication approach

Intragenic alternative splicing coordination is essential for Caenorhabditis elegans slo-1 gene function

Alternative splicing is critical for diversifying eukaryotic proteomes, but the rules governing and coordinating splicing events among multiple alternate splice sites within individual genes are not well understood. We developed a quantitative PCR-based strategy to quantify the expression of the 12 transcripts encoded by the Caenorhabditis elegans slo-1 gene, containing three alternate splice sites. Using conditional probability-based models, we show that splicing events are coordinated across these sites. Further, we identify a point mutation in an intron adjacent to one alternate splice site that disrupts alternative splicing at all three sites. This mutation leads to aberrant synaptic transmission at the neuromuscular junction. In a genomic survey, we found that a UAAAUC element disrupted by this mutation is enriched in introns flanking alternate exons in genes with multiple alternate splice sites. These results establish that proper coordination of intragenic alternative splicing is essential for normal physiology of slo-1 in vivo and identify putative specialized cis-regulatory elements that regulate the coordination of intragenic alternative splicing

Coherent Backscattering of Ultracold Atoms

We report on the direct observation of coherent backscattering (CBS) of ultracold atoms, in a quasi-two-dimensional configuration. Launching atoms with a well-defined momentum in a laser speckle disordered potential, we follow the progressive build up of the momentum scattering pattern, consisting of a ring associated with multiple elastic scattering, and the CBS peak in the backward direction. Monitoring the depletion of the initial momentum component and the formation of the angular ring profile allows us to determine microscopic transport quantities. The time resolved evolution of the CBS peak is studied and is found a fair agreement with predictions, at long times as well as at short times. The observation of CBS can be considered a direct signature of coherence in quantum transport of particles in disordered media. It is responsible for the so called weak localization phenomenon, which is the precursor of Anderson localization.Comment: 5 pages, 4 figure

Challenges in Gluten Analysis: A Comparison of Four Commercial Sandwich ELISA Kits

Gluten is composed of prolamin and glutelin proteins from several related grains. Because these proteins are not present in identical ratios in the various grains and because they have some differences in sequence, the ability to accurately quantify the overall amount of gluten in various food matrices to support gluten-free labeling is difficult. Four sandwich ELISAs (the R-Biopharm AG R5 RIDASCREEN®, the Neogen Veratox® R5, the Romer Labs AgraQuant® G12, and the Morinaga Wheat kits) were evaluated for their performance to quantify gluten concentrations in various foods and ingredients. The Morinaga and AgraQuant® G12 tests yielded results comparable to the two R5 kits for most, but not for certain, foods. The results obtained with the Morinaga kit were lower when compared to the other kits for analyzing powders of buckwheat and several grass-based products. All four kits were capable of detecting multiple gluten-containing grain sources including wheat, rye, barley, semolina, triticale, spelt, emmer, einkorn, Kamut™, and club wheat. Users of the ELISA kits should verify the performance in their hands, with matrices that are typical for their specific uses. The variation in results for some food matrices between test methods could result in trade disputes or regulatory disagreements

Should Aggressive Surgical Local Control Be Attempted in All Patients with Metastatic or Pelvic Ewing's Sarcoma?

In previous reports, patients with Ewing's sarcoma received radiation therapy (XRT) for definitive local control because metastatic disease and pelvic location were thought to preclude aggressive local treatment. We sought to determine if single-site metastatic disease should be treated differently from multicentric-metastatic disease. We also wanted to reinvestigate the impact of XRT, pelvic location, and local recurrence on outcomes. Our results demonstrated a significant difference in overall survival (OS) between patients with either localized disease or a single-metastatic site and patients with multicentric-metastatic disease (P = 0.004). Local control was also found to be an independent predictor of outcomes as demonstrated by a significant difference in OS between those with and without local recurrence (P = 0.001). Axial and pelvic location did not predict a decreased OS. Based on these results, we concluded that pelvic location and the diagnosis of metastatic disease at diagnosis should not preclude aggressive local control, except in cases of multicentric-metastatic disease

Evaluating Potential Risks of Food Allergy and Toxicity of Soy Leghemoglobin Expressed in \u3ci\u3ePichia pastoris\u3c/i\u3e

Scope: The Soybean (Glycine max) leghemoglobin c2 (LegHb) gene was introduced into Pichia pastoris yeast for sustainable production of a heme-carrying protein, for organoleptic use in plant-based meat. The potential allergenicity and toxicity of LegHb and 17 Pichia host-proteins each representing ≥ 1% of total protein in production batches are evaluated by literature review, bioinformatics sequence comparisons to known allergens or toxins, and in vitro pepsin digestion. Methods and results: Literature searches found no evidence of allergenicity or toxicity for these proteins. There are no significant sequence matches of LegHb to known allergens or toxins. Eleven Pichia proteins have modest identity matches to minor environmental allergens and 13 Pichia proteins have significant matches to proteins from toxic sources. Yet the matched allergens and toxins have similar matches to proteins from the commonly consumed yeast Saccharomyces cerevisiae, without evidence of food allergy or toxicity. The demonstrated history of safe use indicates additional tests for allergenicity and toxicity are not needed. The LegHb and Pichia sp. proteins were rapidly digested by pepsin at pH 2. Conclusion: These results demonstrate that foods containing recombinant soy LegHb produced in Pichia sp. are unlikely to present an unacceptable risk of allergenicity or toxicity to consumers

Allergenicity assessment of genetically modified crops—what makes sense?

GM crops have great potential to improve food quality, increase harvest yields and decrease dependency on certain chemical pesticides. Before entering the market their safety needs to be scrutinized. This includes a detailed analysis of allergenic risks, as the safety of allergic consumers has high priority. However, not all tests currently being applied to assessing allergenicity have a sound scientific basis. Recent events with transgenic crops reveal the fallacy of applying such tests to GM crops