An exploration of the effects of group summative assessment marking on higher education students’ overall marks

Groupwork and group summative assessment (GSA) are important learning, teaching and assessment methods used by many educational institutions, not just universities. The differences between the marks that HEI students were awarded for their own independent individual summative assessment (IISA) work and their GSA marks were explored. The study topic presented itself while the author was contemplating studying for a first degree, when it became apparent that group working and group summative assessment was included in summative assessment methods used in the chosen programme. Three data sources were from UK undergraduates and graduates, and one was from Australian PG students. Module marks data were collected from over 4000 HE students. They were divided into eighteen faculty/year data sets from four HEI sources. A systematic difference was found between the distributions of GSA and IISA marks, supporting Lejk et al. (1999). Lower IISA ability students scored higher in GSA modules than in IISA modules. Higher IISA ability students scored lower in GSA modules. In addition, the mean GSA mark was higher than the mean IISA mark. The standard deviation of the GSA marks was lower than the SD of the IISA marks. Both of these findings support Downie (2001). The relationship was found to vary between the data sets, modules, assessment items and especially between faculties. The results and conclusions from this study will empower stakeholders, enabling them to be better informed in their choice of first-degree study programmes. They will also allow the use and impact of GSA to be more transparent and better understood, leading to further research and improvement in practice

The Hunting Hours: London, British Library, MS Egerton 1146

Late medieval Books of Hours have long been a favoured area of research for scholars and much has also been written about the medieval hunt, particularly its aristocratic aspects. However, there has been no real attempt to integrate these two areas of knowledge in order to produce a clearer understanding of personal prayer books, their content, and the motives and personalities of their patrons. This analysis of MS Egerton 1146, a virtually unpublished Book of Hours in the British Library, is an endeavour to remedy this gap

The Study of Fork Processing and DSB Repair in Bacteriophage T4: Roles of the MR Complex and Endonuclease VII

<p><bold>Abstract</bold></p><p>During DNA replication, organisms often encounter different types of DNA damage or lesions that lead to the stalling or breakage of replication forks. Cells must repair this damage or it can lead to genome instability and the loss of cell viability. It is now accepted that recombination plays a vital role in the rescue and restart of replication forks. The study of replication fork processing by recombination is important for understanding how cells avoid DNA damage which, if unrepaired, can lead to cancer or cell death. </p><p>One of the most detrimental forms of DNA damage experienced by the cell is a double-stranded DNA break (DSB). DSBs can be repaired in an error-free manner by homologous recombination through many different proposed mechanisms. Homologous recombination is of particular interest as defects in the recombination proteins often lead to deadly human syndromes characterized by a predisposition to cancer. For example, mutations in the BRCA2 protein, which is involved in homologous recombination for the repair of DSBs, have been linked to an increased incidence of breast cancer. Therefore, our lab is interested in studying the mechanism of homologous recombination and in particular how it relates to stalled fork processing and DSB repair. </p><p>We studied the different characteristics of DNA recombination using bacteriophage T4 as a model system. Phage T4 is a well-characterized virus that infects Escherichia coli. Phage T4 has a simple genome that encodes for almost all of the proteins involved in its own replication, recombination, and repair. At late times of infection, phage T4 predominantly uses recombination-dependent replication (RDR) to replicate its DNA. This recombination-dependent replication system is very similar to homologous recombination used by higher organisms. One major advantage of using phage T4 as a model system is that unlike other model systems, "lethal" mutations can be examined in T4 using strains that suppress specific mutations. Using this system, we investigated a few of the phage T4 proteins (homologous with higher organism proteins) that are thought to be involved in DSB repair and replication fork processing and whose roles are not yet fully defined.</p><p>One such protein that we studied was gene product 47 (gp47). The gp47 protein is a member of the bacteriophage T4 Mre11/Rad50 complex and is homologous to the human Mre11 protein (gp47 will be referred to as T4 Mre11). We investigated the in vivo functions of T4 Mre11 in double-strand end processing, double-strand break repair, and recombination-dependent replication. Specifically, we tested the importance of the conserved histidine residue in nuclease Motif I (catalytic active site) of the T4 Mre11 protein. Substitution with multiple different amino acids (including serine) failed to support phage growth, completely blocked plasmid recombination-dependent replication, and led to the stabilization of double-strand ends. Our collaborator, Dr. Scott Nelson, also constructed and expressed a T4 Mre11 mutant protein with the same conserved histidine changed to serine. The mutant protein was found to be completely defective for nuclease activities, but retained the ability to bind T4 Rad50 and double-stranded DNA. These results indicate that the nuclease activity of T4 Mre11 is critical for phage growth and recombination-dependent replication during T4 infections.</p><p>Another such protein that we studied was gene product 46 (gp46). The gp46 protein is the other member of the bacteriophage T4 Mre11/Rad50 complex and is homologous to the human Rad50 protein (gp46 will be referred to as T4 Rad50). We investigated the in vivo functions of T4 Rad50 in double-strand end processing, double-strand break repair, and recombination-dependent replication. Specifically, we tested different aspects of the protein such as the Signature motif, which is part of the nucleotide binding domain (ATPase active site). Substitution with different ATPase-deficient mutations completely blocked plasmid recombination-dependent replication and led to the stabilization of double-strand ends. These results indicate that the ATPase activity of T4 Rad50 is critical for recombination-dependent replication during T4 infections.</p><p>Finally, we investigated Endonuclease VII (gp49), which is encoded by gene 49. EndoVII was the first endonuclease shown to cleave Holliday junctions in vivo and was shown to cleave stalled replication forks in vitro. We were interested in investigating the proposed regulation of EndoVII expression along with EndoVII's role in replication fork processing. Specifically, we used the EndoVII hairpin mutation to address the proposed regulation of EndoVII expression. In the presence of WT EndoVII we observed only the full-length EndoVII protein, whereas in the presence of the EndoVII hairpin mutant, we observed no EndoVII protein. This result indicates that EndoVII is not regulated to express different length proteins at different times of infection. </p><p>We also used the EndoVII hairpin mutation to investigate EndoVII's role in replication fork processing. We generated phage strains to monitor EndoVII's effect on recombination using a phage x phage recombination assay. We observed no difference in recombination between WT and HP- strains. Taken with the above conclusions, we propose that the EndoVII hairpin mutation disrupts the late promoter and reduces the amount of active EndoVII available. In turn, this would affect the phage's ability to package its DNA due to unresolved recombination junctions and its ability to survive.</p>Dissertatio

Evasion of cytotoxic T lymphocyte (CTL) responses by nef-dependent induction of Fas ligand (CD95L) expression on simian immunodeficiency virus-infected cells.

Published versio

Aggregation and Representation in the European Parliament Party Groups

While members of the European Parliament are elected in national constituencies, their votes are determined by the aggregation of MEPs in multinational party groups. The uncoordinated aggregation of national party programmes in multinational EP party groups challenges theories of representation based on national parties and parliaments. This article provides a theoretical means of understanding representation by linking the aggregation of dozens of national party programmes in different EP party groups to the aggregation of groups to produce the parliamentary majority needed to enact policies. Drawing on an original data source of national party programmes, the EU Profiler, the article shows that the EP majorities created by aggregating MEP votes in party groups are best explained by cartel theories. These give priority to strengthening the EP’s collective capacity to enact policies rather than voting in accord with the programmes they were nationally elected to represent