An event in the iberoamerican cooperation for the conservation and utilization of the zoogenetic resources

Between 1st and 4th of December, 2003; an event was celebrated in the city of Recife (Pernambuco, Brazil) congregating more than 200 researchers of 16 countries, to present results and discuss the last advances in the Iberoamerican Zoogenetics Resources Conservation and Utilization Area, taking into account contents over Genetics, Assisted reproduction, Rural development and Ethnology. In the present editorial a slight introduction is developed to locate the reader in the objectives, the contents and the development of the event, standing out the rule developed by the organizers, the supporters and the contributors.Del uno al cuatro de diciembre de 2003 se celebró en la ciudad de Recife (Pernambuco, Brasil) un evento que congregó a más de 200 investigadores de 16 países, para presentar resultados y discutir los últimos avances en el área de la conservación y utilización de los Recursos Zoogenéticos de Iberoamérica, tratándose contenidos sobre Genética, Reproducción asistida, Desarrollo rural, y Etnología principalmente. En la presente editorial se realiza una somera introducción para ubicar al lector en los objetivos, los contenidos y el desarrollo del evento, resaltando el papel desarrollado por los organizadores, patrocinadores y participantes

Estimation of morphoestructural traits in moxoto breed goats

The raising goat systems in Brazil are characterized as extensive or semi-extensive in the majority of them. In native breeds dominate the extensive system, being common introduction of exotic breed as Anglo Nubian and Boer. In addition, the geographic isolation difficulty change of sires, promote genetic isolation and changes in breed pattern, consequently. The objective of the present work were to evaluate eleven quantitative morphoestructural traits: raised to the withers (RW), raised to the entrance of croup (RC), thoracic perimeter (PT), width of croup (WC), length of croup (LC), width of head (WH), face length (FL), width of face (WF), ear size (ES), shin circumference of the cinnamon (CCi) of 313 Moxoto animal breed of several municipality of Pernambuco and Paraíba States, northeastern, semi arid region of Brazil. According to canonical analyses it is possible to verify diferentiation and sub populations formation.Os sistemas de produção de caprinos no Brasil caracterizam-se na sua maioria como extensivo ou semi-extensivo. Porém, na criação de caprinos nativos predomina o sistema ultra-extensivo, sendo comum a introdução de animais exóticos, principalmente das raças Anglo Nubiana e Boer para cruzamento com o rebanho local, fato que tem contribuído para diluição genética e perda das características raciais.. Além disso, o isolamento geográfico a que estão submetidos, dificulta a troca de reprodutores entre rebanhos, promove isolamento genético e como conseqüência final, mudanças no padrão racial. O presente trabalho teve como objetivo utilizar onze variáveis morfo-estruturais de natureza quantitativas, tais como: longitude da cabeça (LC); longitude do rosto (LR); largura da cabeça (LCa); comprimento do corpo (CC); perímetro torácico (PT); altura da cernelha (AC); altura da região sacral (ARS); largura da garupa (LG); longitude da garupa (LGa); perímetro da canela (PCa) e; tamanho da orelha (TO) de 313 animais da raça caprina Moxotó, de diferentes municípios dos Estados de Pernambuco e Paraíba, região semi-árida do Nordeste brasileiro. Pela análise canônica foi possível verificar diferenciação e formação de sub-populações dentro da raça

Damage localisation in beams using the ritz method and speckle shear interferometry

A novel numerical-experimental technique is developed in order to minimise some of the difficulties exhibited by others damage localisation approaches. The present technique relies on the computation of undamaged rotation fields using the Ritz method and the Timoshenko beam theory, while the measurement of damaged rotation fields is performed by speckle shear interferometry. Two damage localisation indicators are also presented, which, instead of being based on the second derivative of displacement fields, are based on the first spatial derivative of rotation fields. These damage localisation indicators, the modified curvature difference (MCD) and the modified damage index (MDI), were applied successfully in the localisation of damage in two clamped-clamped aluminium beams.The authors greatly appreciate the financial support of FCT through Project FCT PTDC/EME-PME/102095/2008

Efficacy of free and nanoencapsulated Eucalyptus citriodora essential oils on sheep gastrointestinal nematodes and toxicity for mice

AbstractHerbal medicines with anthelmintic effects are alternatives for the sustainable control and prevention of disease caused by gastrointestinal parasites. The nanoencapsulation of essential oils has been proposed to enhance the absorption of their constituents and improve their efficacy. The present study aimed to evaluate the efficacy of free and nanoencapsulated Eucalyptus citriodora essential oil (EcEO) on the control of gastrointestinal nematodes of small ruminants in vitro and in vivo. Chitosan was used as a matrix for the formulation of a nanoemulsion. Chromatographic and physico-chemical analyses of EcEO were performed. Egg hatch (EHT) and larval development (LDT) tests were conducted to evaluate the effectiveness of nanoencapsulated and free EcEO on the eggs and larvae of Haemonchus contortus. Acute toxicity of free and nanoencapsulated EcEO was evaluated using mice. Finally, nanoencapsulated EcEO efficacy on the control of gastrointestinal nematodes was calculated by fecal egg count reduction test (FECRT) treating 30 sheep naturally infected with 250mg/kg of free and nanoencapsulated EcEO. In vitro tests were analyzed by an analysis of variance (ANOVA) followed by comparison with the Tukey test. The efficacy of FECRT was calculated by the BootStreet program through arithmetic average, using the formula 100 (1−XT/XC). To compare the differences between epg, the data were transformed to log(x+1) and subjected to an ANOVA to compare the significant differences between groups by Tukey's. The level of significance was P<0.05. The free (4mg/ml concentration) and nanoencapsulated (2mg/ml concentration) EcEO inhibited larvae hatching by 97.2% and 92.8%, respectively. Free and nanoencapsulated EcEO at 8mg/ml inhibited larval development by 99.8% and 98.1%, respectively. In the acute toxicity test, the LD10 and LD50 of free EcEO was 1999 and 2653mg/kg, respectively, while the LD10 and LD50 of nanoencapsulated EcEO was 1121 and 1681mg/kg, respectively. Nanoencapsulated and free EcEO reduced FEC similarly by 40.5% and 55.9%, respectively at 10 days post-treatment. Nanoencapsulated EcEO did not obtain the expected efficacy in vivo

Superconductivity in MgB_2 doped with Ti and C

Measurements of the superconducting upper critical field, H_{c2}, and critical current density, J_c, have been carried out for MgB_2 doped with Ti and/or C in order to explore the problems encountered if these dopants are used to enhance the superconducting performance. Carbon replaces boron in the MgB_2 lattice and apparently shortens the electronic mean free path thereby raising H_c2. Titanium forms precipitates of either TiB or TiB_2 that enhance the flux pinning and raise J_c. Most of these precipitates are intra-granular in the MgB_2 phase. If approximately 0.5% Ti and approximately 2% C are co-deposited with B to form doped boron fibers and these fibers are in turn reacted in Mg vapor to form MgB_2, the resulting superconductor has H_{c2}(T=0) ~ 25 T and J_c ~ 10,000 A/cm**2 at 5 K and 2.2 T.Comment: 11 pages, 10 figure

Perspetivas de utilização de nanomateriais em nanodiagnóstico

Conventional diagnostic methods are exclusively focused on the detection of the symptoms associated to a given pathology. Any process able to determine with anticipation the occurrence of a disease, inclusively before the first symptoms, or providing the means to predict the possibility of occurrence of that disease, would significantly reinforce the effectiveness of the therapy allowing, at the same time, the establishment of prevention measures to avoid its progression. Much of the worldwide health research aims at the development of expedite devices or strategies capable of carrying out the monitoring of first level signs, or able to early detect and quantify biomarkers, usually at very low concentrations, associated to a given illness. In the search for alternative diagnostic methods able to overcome the limitations of available technology nanomaterials assume a decisive role and anticipate extremely promising developments in healthcare medicine. Os métodos de diagnósticos convencionais estão exclusivamente empenhados na deteção de sintomas associados a uma dada patologia. Qualquer processo que permita determinar o mais antecipadamente possível a ocorrência da doença, inclusive antes de surgirem os primeiros indícios, ou que permita avaliar o risco da doença ocorrer, reforçaria significativamente as hipóteses de sucesso da terapia e tornaria até praticável tomar medidas preventivas para evitar a sua progressão. Muita da investigação em curso na área da medicina passa pelo desenvolvimento de estratégias ou dispositivos capazes de efetuar a monitorização, o mais antecipadamente possível, destes indícios, ou então capazes de detetar e quantificar precocemente marcadores biológicos, usualmente em concentrações muito baixas, associados a uma dada patologia. Na pesquisa por métodos de diagnósticos alternativos, aptos a ultrapassar algumas das limitações dos métodos atuais, os nanomateriais assumem um papel decisivo e abrem perspetivas de desenvolvimento extremamente promissoras para a área do nanodiagnóstico.

É a Murciano-Granadina raça única? Um enfoque genético-molecular

The population structure of the Murciano-Granadina breed was determined using 25 microsatellites from 266 goats of seven populations. The results of the genetic differentiation analysis showed that it is possible to differentiate the Murciana and Granadina populations even though a low FST value (0.0432) had been obtained. Individuals could be assigned to their populations with a success rate of more than 80%. Bayesian-based clustering analysis of allele frequencies and multivariate analysis revealed that Murciana and Granadina populations were grouped in different clusters since K=3. The results demonstrate that Murciana and Granadina are still two different genetic groups included into Murciano-Granadina denomination. There is the opportunity to the genetically manage these populations, under a single herd-book but adding the necessary modifications to respect the conservation of the genetic diversity based on the use of multibreed models of genetic evaluationDeterminou-se a estrutura da raça Murciano-Granadina, usando-se 25 microssatélites e 266 animais de sete populações. Os resultados da diferenciação genética mostram que é possível diferenciar populações de Murciana e Granadina, apesar dos baixos valores de FST obtidos – 0.0432. Os indivíduos foram designados às suas populações com taxa de sucesso superior a 80%. A análise bayesiana de agrupamento das frequências alélicas e a análise multivariada revelaram que as populações Murciana e Granadina foram agrupadas em diferentes clusters, uma vez que o melhor K obtido foi três. Os resultados demonstraram que Murciana e Granadina ainda são dois grupos genéticos distintos incluídos na denominação Murciano-Granadina. É possível manejar geneticamente essas populações dentro de um único livro de registro, porém adotando-se as modificações necessárias em relação à conservação e à diversidade genética, com base no uso de modelos de avaliação multirracia

Accuracy of implant casts generated with conventional and digital Impressions: an in vitro study

Purpose: The aim of this study was to compare the accuracy of digital dental impressions with the accuracy of impressions obtained via conventional techniques. Methods: Two different master models were created, one with parallel implants (model 1) and the other with non-parallel implants (model 2). These reference master models included 4 Klockner KL RP implants (Klockner Implant System SA, Barcelona, Spain), which were juxta-placed and equidistant in the intermentoneal region. In model 1 the implants were placed parallel to each other, whereas in model 2 the implants were placed such that there was a divergence angle of 15° between the more distal implants, and a convergence angle of 15° between the two central implants. A total of four types of impressions were obtained from model 1 (four groups, n = 10 each), including closed tray impressions with replacement abutments; open tray impression groups for dragging copings, without splinting; open tray impressions for ferrules; and impressions obtained using the 3MTM True Definition Scanner system. For model 2 three groups were created (three groups, n = 10 each), including closed tray impressions with replacement abutments; open tray impression for dragging copings, without splinting; and impressions obtained using the 3MTM True Definition Scanner system. The master models and the models obtained using conventional methods were digitalized in order to compare them via an extraoral high-resolution scanner (Imetric IScan D104i, Porretruy, Switzerland). The STL (Stereo Lithography (format for transferring 3 dimensional shape information)) digital values were loaded into reverse-engineering software and superimposed with their respective STL master models in order to evaluate deviations in three dimensions. We then analyzed the squares of the deviations in the three axes and evaluated the median and the sum of the deviation square. Statistical analysis was performed using the IBM Corp. Released 2016. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp. The normality of the distributions was analyzed according to a Kolmogorov-Smirnov test. The median comparison was performed using the differences between the medians, analyzed using non-parametric Kruskal-Wallis and Mann-Whitney tests with a significance level of p < 0.05. Results: For model 1, the deviations of the digital impressions were smaller than those associated with the conventional techniques. The sum value in group D was 1,068,292, which was significantly lower than those of groups A, B, and C, which were shown to be 2,114,342, 2,165,491, and 1,265,918, respectively. This improvement was not observed when using model 2, however, where the conventional techniques yielded similar results. Group F simultaneously presented the lowest total square sum of the three deviations (1,257,835), indicating a significantly higher accuracy for this group in model 2, while the sum values were 1,660,975 and 1,489,328 for groups E and G, respectively. Conclusion: Digital impressions of full-arch models were able to achieve the accuracy of conventional impressions in an in vitro model. Nevertheless, further in vivo studies are needed to validate these in vitro results

Multivariate Study Of Nile Tilapia Byproducts Enriched With Omega-3 And Dried With Different Methods

The present work aimed at studying the effect of different drying methods applied to tilapia byproducts (heads, viscera and carcasses) fed with flaxseed, verifying the contents of omega-3 fatty acids. Two diets were given to the tilapia: a control and a flaxseed formulation, over the course of 60 days. After this period, they were slaughtered and their byproducts (heads, viscera and carcasses) were collected. These fish parts were analyzed in natura, lyophilized and oven dried. Byproducts from tilapia fed with flaxseed presented docosapentaenoic, eicopentaenoic and docosahexanoic fatty acids as a result of the enzymatic metabolism of the fish. The byproducts from the oven drying process had lower levels of polyunsaturated fatty acids. In the multivariate analysis, the byproducts from fish fed with flaxseed had a greater composition of fatty acids. The addition of flaxseed in fish diets, as well as the utilization of their byproducts, may become a good business strategy. Additionally, the byproducts may be dried to facilitate transport and storage. © 2016 Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.361182

Cloning and sequence analysis of the gene encoding Methylophilus methyltrophus cytochrome c: a unique protein with a perpendicular orientation of the histidinyl ligands

Metals in Catalysis, Biomimetics & Inorganic Material