Ms. Codex 176: “Pastorcillos” or “Little Shepherds”: Exploring a Miscellany Manuscript from Spain’s Early Modern Period

An investigation and partial transcription of Pastorcillos, a seventeenth century play written in old Castilian Spanish and in Catalan. This thesis explores the possible origins and genre of this play.https://repository.upenn.edu/manuscript_collective_transcription/1002/thumbnail.jp

ANALISIS SEKUENS Gen matK Sansevieria trifasciata var. Laurentii DAN var. Hahnii

ANALISIS SEKUENS Gen matK Sansevieria trifasciata var. Laurentii DAN var. Hahnii   Riano Rembet1), Johanis J. Pelealu1), Beivy J. Kolondam1), Trina E. Tallei1*) 1)Jurusan Biologi FMIPA Universitas Sam Ratulangi * [email protected]   ABSTRACT   Sansevieria has many hybrids and horticultural varieties so that the classification of this plant in the genus is often difficult to do. In the Sansevieria genera, S. trifasciata is the most commercialized species. There are at least 20 cultivars of this species that have been sold around the world. Two varieties among S. trifasciata collected by ornamental plant collectors are var. Laurentii and var. Hahnii. Morphological characters of both plants are very distinct, so they may be considered as different species. Lack of information about the genetic variation in S. trifasciata encouraged this study. This study aimed to assess differences in matK gene sequences between S. trifasciata var. Laurentii and var. Hahnii, as well as to compare them with matK sequence of S. trifasciata obtained from GenBank. Total DNA was extracted from fresh leaves using innuPREP Plant DNA Kit (Analytik Jena) in accordance with the protocol provided. DNA obtained was used for the PCR process to obtain the matK gene fragment. Primer pairs used for amplification and gene sequencing were 3F-r and 1R-f. The results showed that there were no differences among matK sequences of S. trifasciata var. Laurentii, S. trifasciata var. Hahnii, and S. trifasciata obtained from GenBank. In conclusion, those plants are the same species. Keywords: matK gene, hybrid, Sansevieria trifasciata var. Laurentii, Sansevieria trifasciata var. Hahnii, genetic variation, variety ABSTRAK Sansevieria memiliki banyak sekali hibrida dan varietas hortikultur sehingga klasifikasi tumbuhan ini di dalam genusnya seringkali sulit sekali dilakukan. Di dalam genus Sansevieria, S. trifasciata merupakan spesies yang paling dikomersilkan. Paling tidak terdapat 20 kultivar dari spesies ini yang telah dijual di seluruh dunia. Dua di antara varietas S. trifasciata yang banyak dikoleksi oleh para kolektor Sansevieria yaitu S. trifasciata var. Laurentii dan var. Hahnii. Karakter morfologi kedua tumbuhan ini sangat berbeda sehingga terdapat kemungkinan dianggap sebagai spesies yang berbeda. Kurangnya informasi mengenai variasi genetik pada S. triafsciata mendorong dilakukannya penelitian ini. Penelitian ini bertujuan untuk menganalisis sekuens gen matK S. trifasciata var. Laurentii dan S. trifasciata var. Hahnii, serta membandingkannya dengan S. trifasciata yang diperoleh dari GenBank. DNA total diekstraksi dari daun segar menggunakan innuPREP Plant DNA Kit (Analytik Jena) sesuai dengan protokol yang telah disediakan. DNA yang diperoleh digunakan untuk proses PCR untuk mendapatkan fragmen gen matK. Pasangan primer matK yang digunakan untuk amplifikasi dan sekuensing yaitu 3F-r dan 1R-f. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan sekuens matK antara S. trifasciata var. Laurentii dan S. trifasciata var. Hahnii, maupun dengan S. trifasciata yang ada di GenBank. Kesimpulan dari penelitian ini yaitu tumbuhan-tumbuhan ini merupakan spesies yang sama.   Kata kunci: gen matK, hibrida, Sansevieria trifasciata var. Laurentii, Sansevieria trifasciata var. Hahnii, variasi genetik, varieta

Political Tolerance, Culture, and the Individual

https://digitalcommons.cedarville.edu/alum_books/1340/thumbnail.jp

Political Tolerance, Culture, and the Individual

https://digitalcommons.cedarville.edu/faculty_books/1107/thumbnail.jp

Is it possible that the Goldbach's and Twins primes conjectures are true with an algebraic approach?

In this paper, using an algebraic approach, it is intended to show that the Goldbach's and Twin primes conjectures are true, building, for each $m>2$, an isomorphism between posets. One of the posets is the set of coprimes less than $m$, while the other is endowed with an operation that grants it an abelian group structure. Special features of this operation are demonstrated in the document, which allow characterizing the even numbers, as if they were their fingerprint; furthermore, such an operation locates, in a natural way, the pairs that satisfy the conjecture. Moreover, an algorithm that generates pairs of numbers that satisfy the conjecture is presented, and examples of some of the beautiful symmetries of the orbits of cyclic subgroups are shown, for the proposed abelian group

An optimal convergent Collatz algorithm

In this research an optimal algorithm of the Collatz conjecture is proposed, and the convergence of the application is demonstrated. In addition, the result is related to the classical Collatz conjecture, presenting a possible important proof and an equation that relates them

PENGARUH PEMAHAMAN PAJAK, KUALITAS PELAYANAN DAN SANKSI PAJAK TERHADAP KEPATUHAN PAJAK (Studi Empiris Pada WP OP yang Melakukan Kegiatan Usaha di KPP Pratama Semarang Candisari)

This study as a purpose to analyze the influence of the understanding of taxpayers, quality of service, and tax penalties on tax compliance. Attribution theory explains that the internal effect caused by an individual's personal conduct which is the understanding of taxpayers on tax compliance, while external influences are thought to result from an outside party that is in terms of quality of service tax authorities and tax penalties provided by the government. Based on social learning theory proposed by Albert Bandura (1977), this theory should conduct direct observation and experience to participate in terms of tax compliance. Respondents in this study is an individual taxpayer who carries on business in Semarang Candisari STO. The analysis technique used is the technique of multiple regression analysis with the data obtained using quantitative methods are surveys using a questionnaire to the media which is convenience sampling technique. Total number of questionnaires were analyzed by 114 questionnaires. This study shows that the understanding of tax, service quality and the tax authorities, and the sanctions applicable taxes and significant positive effect on tax compliance