Electron energy-loss spectrometry on lithiated graphite

Transmission electron energy-loss spectrometry was used to investigate the electronic states of metallic Li and LiC6, which is the Li-intercalated graphite used in Li-ion batteries. The Li K edges of metallic Li and LiC6 were nearly identical, and the C K edges were only weakly affected by the presence of Li. These results suggest only a small charge transfer from Li to C in LiC6, contrary to prior results from surface spectra obtained by x-ray photoelectron spectroscopy. Effects of radiation damage and sample oxidation in the transmission electron microscopy are also reported

Dose, exposure time, and resolution in Serial X-ray Crystallography

The resolution of X-ray diffraction microscopy is limited by the maximum dose that can be delivered prior to sample damage. In the proposed Serial Crystallography method, the damage problem is addressed by distributing the total dose over many identical hydrated macromolecules running continuously in a single-file train across a continuous X-ray beam, and resolution is then limited only by the available molecular and X-ray fluxes and molecular alignment. Orientation of the diffracting molecules is achieved by laser alignment. We evaluate the incident X-ray fluence (energy/area) required to obtain a given resolution from (1) an analytical model, giving the count rate at the maximum scattering angle for a model protein, (2) explicit simulation of diffraction patterns for a GroEL-GroES protein complex, and (3) the frequency cut off of the transfer function following iterative solution of the phase problem, and reconstruction of an electron density map in the projection approximation. These calculations include counting shot noise and multiple starts of the phasing algorithm. The results indicate counting time and the number of proteins needed within the beam at any instant for a given resolution and X-ray flux. We confirm an inverse fourth power dependence of exposure time on resolution, with important implications for all coherent X-ray imaging. We find that multiple single-file protein beams will be needed for sub-nanometer resolution on current third generation synchrotrons, but not on fourth generation designs, where reconstruction of secondary protein structure at a resolution of 0.7 nm should be possible with short exposures.Comment: 19 pages, 7 figures, 1 tabl

Constraining the neutrino magnetic moment with anti-neutrinos from the Sun

We discuss the impact of different solar neutrino data on the spin-flavor-precession (SFP) mechanism of neutrino conversion. We find that, although detailed solar rates and spectra allow the SFP solution as a sub-leading effect, the recent KamLAND constraint on the solar antineutrino flux places stronger constraints to this mechanism. Moreover, we show that for the case of random magnetic fields inside the Sun, one obtains a more stringent constraint on the neutrino magnetic moment down to the level of \mu_\nu \lsim few \times 10^{-12}\mu_B, similar to bounds obtained from star cooling.Comment: 4 pages, 3 figures. Final version to appear in Phys. Rev. Let

Leukocyte Associated Immunoglobulin Like Receptor 1 Regulation and Function on Monocytes and Dendritic Cells During Inflammation

Inhibitory receptors are crucial immune regulators and are essential to prevent exacerbated responses, thus contributing to immune homeostasis. Leukocyte associated immunoglobulin like receptor 1 (LAIR-1) is an immune inhibitory receptor which has collagen and collagen domain containing proteins as ligands. LAIR-1 is broadly expressed on immune cells and has a large availability of ligands in both circulation and tissues, implicating a need for tight regulation of this interaction. In the current study, we sought to examine the regulation and function of LAIR-1 on monocyte, dendritic cell (DC) and macrophage subtypes, using different in vitro models. We found that LAIR-1 is highly expressed on intermediate monocytes as well as on plasmacytoid DCs. LAIR-1 is also expressed on skin immune cells, mainly on tissue CD14(+) cells, macrophages and CD1c(+) DCs. In vitro, monocyte and type-2 conventional DC stimulation leads to LAIR-1 upregulation, which may reflect the importance of LAIR-1 as negative regulator under inflammatory conditions. Indeed, we demonstrate that LAIR-1 ligation on monocytes inhibits toll like receptor (TLR)4 and Interferon (IFN)-alpha- induced signals. Furthermore, LAIR-1 is downregulated on GM-CSF and IFN-gamma monocyte-derived macrophages and monocyte-derived DCs. In addition, LAIR-1 triggering during monocyte derived-DC differentiation results in significant phenotypic changes, as well as a different response to TLR4 and IFN-alpha stimulation. This indicates a role for LAIR-1 in skewing DC function, which impacts the cytokine expression profile of these cells. In conclusion, we demonstrate that LAIR-1 is consistently upregulated on monocytes and DC during the inflammatory phase of the immune response and tends to restore its expression during the resolution phase. Under inflammatory conditions, LAIR-1 has an inhibitory function, pointing toward to a potential intervention opportunity targeting LAIR-1 in inflammatory conditions

Semaphorin4A-Plexin D1 Axis Induces Th2 and Th17 While Represses Th1 Skewing in an Autocrine Manner

Semaphorin (Sema)4A is a transmembrane glycoprotein that is elevated in several autoimmune diseases such as systemic sclerosis, rheumatoid arthritis and multiple sclerosis. Sema4A has a key role in the regulation of Thelper Th1 and Th2 differentiation and we recently demonstrated that CD4(+) T cell activation induces the expression of Sema4A. However, the autocrine role of Sema4A on Th cell differentiation remains unknown. Naive Th cells from healthy controls were cell sorted and differentiated into Th1, Th2 and Th17 in the presence or absence of a neutralizing antibody against the Sema4A receptor PlexinD1. Gene expression was determined by quantitative PCR and protein expression by ELISA and flow cytometry. We found that the expression of Sema4A is induced during Th1, Th2 and Th17 differentiation. PlexinD1 neutralization induced the differentiation of Th1 cells, while reduced the Th2 and Th17 skewing. These effects were associated with an upregulation of the transcription factor T-bet by Th1 cells, and to downregulation of GATA3 and RORgammat in Th2 cells and Th17 cells, respectively. Finally, PlexinD1 neutralization regulates the systemic sclerosis patients serum-induced cytokine production by CD4(+) T cells. Therefore, the autocrine Sema4A-PlexinD1 signaling acts as a negative regulator of Th1 skewing but is a key mediator on Th2 and Th17 differentiation, suggesting that dysregulation of this axis might be implicated in the pathogenesis of CD4(+) T cell-mediated diseases

Enhanced solar anti-neutrino flux in random magnetic fields

We discuss the impact of the recent KamLAND constraint on the solar anti-neutrino flux on the analysis of solar neutrino data in the presence of Majorana neutrino transition magnetic moments and solar magnetic fields. We consider different stationary solar magnetic field models, both regular and random, highlighting the strong enhancement in the anti-neutrino production rates that characterize turbulent solar magnetic field models. Moreover, we show that for such magnetic fields inside the Sun, one can constrain the intrinsic neutrino magnetic moment down to the level of mu_nu lessthan few times 10^-12 x mu_B irrespective of details of the underlying turbulence model. This limit is more stringent than all current experimental sensitivities, and similar to the most stringent bounds obtained from stellar cooling. We also comment on the robustness of this limit and show that at most it might be weakened by one order of magnitude, under very unlikely circumstances.Comment: 25 pages, 5 figures; final version to appear in Phys. Rev.

Neutrino conversions in random magnetic fields and ν~e\tilde{\nu}_e from the Sun

The magnetic field in the convective zone of the Sun has a random small-scale component with the r.m.s. value substantially exceeding the strength of a regular large-scale field. For two Majorana neutrino flavors $\times$ two helicities in the presence of a neutrino transition magnetic moment and nonzero neutrino mixing we analyze the displacement of the allowed ($\Delta m^2- \sin^22\theta$)-parameter region reconciled for the SuperKamiokande(SK) and radiochemical (GALLEX, SAGE, Homestake) experiments in dependence on the r.m.s. magnetic field value $b$, or more precisely, on a value $\mu b$ assuming the transition magnetic moment $\mu = 10^{-11}\mu_B$. In contrast to RSFP in regular magnetic fields we find an effective production of electron antineutrinos in the Sun even for small neutrino mixing through cascade conversions $\nu_{eL}\to \nu_{\mu L}\to \tilde{\nu}_{eR}$, $\nu_{eL}\to \nu_{\mu R}\to \tilde{\nu}_{eR}$ in a random magnetic field that would be a signature of the Majorana nature of neutrino if $\tilde{\nu}_{eR}$ will be registered. Basing on the present SK bound on electron antineutrinos we have also found an excluded area in the same $\Delta m^2,~\sin^22\theta$-plane and revealed a strong sensitivity to the random magnetic field correlation length $L_0$.Comment: LaTex 36 pages including 14 PostScript figure

The Nature of the Near-IR Core Source in 3C 433

We report the analysis of near-infrared imaging, polarimetric and spectroscopic observations of the powerful radio galaxy 3C433, obtained with the HST and UKIRT telescopes. The high spatial resolution of HST allows us to study the near-nuclear regions of the galaxy (<1 kpc). In line with previous observations, we find that 3C433 has an unresolved core source that is detected in all near-IR bands, but dominates over the host galaxy emission at 2.05 um. Our analysis reveals: (1) the presence of a dust lane aligned close to perpendicular (PA=70\pm5\degr) to the inner radio jet axis (PA=-12\pm2\degr); (2) a steep slope to the near-IR SED ($\alpha=5.8\pm0.1$; F$_{\nu}\propto\nu^{-\alpha}$); (3) an apparent lack of broad permitted emission lines at near-IR wavelengths, in particular the absence of a broad Pa$\alpha$ emission line; and (4) high intrinsic polarization for the unresolved core nuclear source ($8.6\pm1$ per cent), with an E-vector perpendicular (PA=83.0\pm 2.3\degr) to the inner radio jet. Using five independent techniques we determine an extinction to the compact core source in the range 3<A_V<67 mag. An analysis of the long wavelength SED rules out a synchrotron origin for the high near-IR polarization of the compact core source. Therefore, scattering and dichroic extinction are plausible polarizing mechanisms, although in both of these cases the broad permitted lines from the AGN are required to have a width >10^4 km/s (FWHM) to escape detection in our near-IR spectrum. Dichroic extinction is the most likely polarization mechanism because it is consistent with the various available extinction estimates. In this case, a highly ordered, coherent toroidal magnetic field must be present in the obscuring structure close to the nucleus.Comment: 12 pages, 5 figures, accepted for publication in MNRA