The Aggression Questionnaire: A validation study in student samples.

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Encapsulación de la piperine presente en la especie Piper tuberculatum utilizando vesículas multilamelares y determinación de su poder antioxidante

The piperine is an alkaloid present in the vegetal species pipilongo (Piper tuberculatum). Antioxidant properties are associated to this plant. It was encapsulated in multilamellar vesicles (MLV). For that, the validation of the extraction methodology through calibration curves of standard piperine by high resolution liquid chromatography (HPLC) was performed, using a column of C18, mobile phase MetOH: H2O (70:30) and detected by UV to 340nm with a fluency rate of 0.7 mL/ min. The lineal and non-lineal ranges were found between 1 and 300 ppm, with R2 of 0.995. During the extraction of the pipilongo fruit, the effect of two factors, solvents and ultrasound power, was studied, for the optimization of conditions and higher extraction of piperine in pipilongo. The maximum concentration of piperine (246.50 ppm) was found, using ethanol as extraction solvent. The piperine was isolated from its extract, using a solution of KOH with 10 % ethanol. It was purified and characterized by melting point, IR, GC-MS y RMN 1H. Once the extract was obtained, it was proceeded to the formation of liposomes. The phosphatidylcholine and cholesterol vesicles were prepared with extract and with piperine at different concentrations and hydrated with buffer pH 7.40. The average size of the particles formed, measured by dynamic light scattering (DLS), was in a range of 299.7–531.9 nm. For the study of antioxidant activity, a solution of DPPH 0.2 mM was prepared and a reaction was carried out with the extract and piperine liposomes and their corresponding free forms. The IC50 calculated for the extract and piperine were 2.8±0.2 and 21333±1499 ppm, respectively. These values of IC50 were compared against a standard of reference Quercetin, prepared under the same conditions. The test with DPPH showed that the released form possesses greater antioxidant activity while in encapsulation form, it attenuates and stabilizes this capacity.La piperina, alcaloide presente en la especie vegetal pipilongo (Piper tuberculatum) a la cual se le asocian propiedades antioxidantes, fue encapsulada en vesículas multilamelares (MLV). Para ello se realizó la validación de la metodología de extracción a través de curvas de calibración de piperina estándar por cromatografía líquida de alta resolución (HPLC) utilizando una columna C18, fase móvil MetOH:H2O (70:30) y detectada por UV a 340 nm con una tasa de flujo de 0.7 mL/min. El rango lineal y linealidad fueron halladas entre 1 a 300 ppm, con R2 de 0.995. En la extracción del fruto de pipilongo se estudió el efecto de dos factores, disolventes y potencia de ultrasonido, para la optimización de condiciones y mayor extracción de piperina en pipilongo. La máxima concentración de piperina (246.50 ppm) fue encontrada en las condiciones de ultrasonido (US 20 KHz a 175W, 40 min) utilizando etanol como disolvente de extracción. Del extracto, se aisló la piperina utilizando una solución de KOH 10% etanólico. Fue purificada y caracterizada mediante punto de fusión, IR, GC-MS y RMN 1H. Una vez obtenido el extracto, se procedió a la formación de liposomas. Las vesículas de fosfatidilcolina y colesterol fueron preparadas con extracto y con piperina a diferentes concentraciones e hidratadas con buffer PBS a pH 7.40. El tamaño promedio de las partículas formadas, medido por dispersión de luz dinámica (DLS), estuvo en un rango de 299.7–531.9 nm. Para el estudio de la actividad antioxidante, se preparó una solución de DPPH 0.2 mM y se llevó a reacción con los liposomas de extracto y de piperina y sus correspondientes formas libres durante 60 min, medidos a 520 nm. El IC50 calculado para el extracto y la piperina fueron 2.8±0.2 y 21333±1499 ppm, respectivamente. Estos valores de IC50 fueron comparados frente a un estándar de referencia preparado bajo las mismas condiciones, quercetina (IC50 2.5±0.1 ppm). El ensayo con DPPH mostró que la forma liberada posee mayor actividad antioxidante mientras que en la encapsulación atenúa y estabiliza esta capacidad

Development and psychometric properties of the stressors in Breast Cancer Scale.

Background: A diagnosis of breast cancer generates psychological stress, due not only to treatment and its side effects but also to the impact on different areas of the patient’s daily life. Although there are instruments for measuring psychological stress in the cancer context, there is currently no tool for assessing stressors specific to breast cancer. Aims: The aim of this study was to develop the Stressors in Breast Cancer Scale (SBCS). Method: A panel of experts evaluated the clarity and relevance of scale items, providing validity evidence based on test content. Psychometric properties of the scale were then analyzed. Results: Validity evidence based on the internal structure of the SBCS was obtained through exploratory factor analysis (EFA) and confirmatory factor analysis (CFA), following a cross-validation strategy. The CFA supported a second-order factor model with five dimensions: physical appearance and sex strains, health and daily difficulties, interpersonal relationship strains, healthcare strains, and worries and concerns about the future. This structure was invariant across two groups distinguished by time from cancer diagnosis (less than 3 and 3 years or more from diagnosis). Reliability, based on McDonald’s omega and Cronbach’s alpha coefficients, ranged from 0.83 to 0.89 for factor scores, and reached 0.95 for total scores. Validity evidence was also provided by correlations with depression, anxiety, perceived stress, and perceived health and quality of life. Discussion: The results support the use of the SBCS for measuring stress as a stimulus in the breast cancer context. Implications for clinical practice and research are discussed.This study was supported by Grupo de investigación consolidado CTS-110, Junta de Andalucía y I Plan Propio y Transferencia de la Universidad de Málaga (ayuda de Publicación en Abierto, C.1

Movilización de conciencia comunitaria: Una experiencia de Investigación Acción Participativa

17 p.La investigación que se presenta a continuación tuvo dos fases, con sus respectivos objetivos: primero, los objetivos de diagnóstico, orientados a conocer las necesidades, problemáticas y potencialidades de la comunidad de la vereda El Charquito, para realizar junto con ellos el diagnóstico psicosocial; y, segundo, los objetivos de intervención, orientados a fomentar la participación y el compromiso de los actores internos de la vereda El Charquito para transformar de forma positiva las problemáticas identificadas en el diagnóstico de necesidades.Introducción Método Resultados y discusión Construcción de categorías inductivas o emergentes Alcance de la intervención en la comunidad Construcción de conocimiento con la comunidad Ámbitos de transformación en la vereda El Charquito Conclusiones y aportes Referencia

Development and implementation of a web-enabled 3D consultation tool for breast augmentation surgery based on 3D-image reconstruction of 2D pictures

Producing a rich, personalized Web-based consultation tool for plastic surgeons and patients is challenging

Catalytic Decomposition of n-C-7 Asphaltenes Using Tungsten Oxides-Functionalized SiO2 Nanoparticles in Steam/Air Atmospheres

A wide range of technologies are being developed to increase oil recovery, reserves, and perform in situ upgrading of heavy crude oils. In this study, supported tungsten oxide nanoparticles were synthesized, characterized, and evaluated for adsorption and catalytic performance during wet in situ combustion (6% of steam in the air, in volumetric fraction) of n-C-7 asphaltenes. Silica nanoparticles of 30 nm in diameter were synthesized using a sol-gel methodology and functionalized with tungsten oxides, using three different concentrations and calcination temperatures: 1%, 3%, 5% (mass fraction), and 350 degrees C, 450 degrees C, and 650 degrees C, respectively. Equilibrium batch adsorption experiments were carried out at 25 celcius with model solutions of n-C-7 asphaltenes diluted in toluene at concentrations from 100 mg center dot L-1 to 2000 mg center dot L-1, and catalytic wet in situ combustion of adsorbed heavy fractions was carried out by thermogravimetric analysis coupled to FT-IR. The results showed improvements of asphaltenes decomposition by the action of the tungsten oxide nanoparticles due to the reduction in the decomposition temperature of the asphaltenes up to 120 degrees C in comparison with the system in the absence of WOX nanoparticles. Those synthesis parameters, such as temperature and impregnation dosage, play an important role in the adsorptive and catalytic activity of the materials, due to the different WOX-support interactions as were found through XPS. The mixture released during the catalyzed asphaltene decomposition in the wet air atmosphere reveals an increase in light hydrocarbons, methane, and hydrogen content. Hydrogen production was prioritized between 300 and 400 degrees C where, similarly, the reduction of CO, CH4, and the increase in CO2 content, associated with water-gas shift, and methane reforming reactions occur, respectively. The results show that these catalysts can be used either for in situ upgrading of crude oil, or any application where heavy fractions must be transformed

Enzymatic Cross-Linking of Alkali Extracted Arabinoxylans: Gel Rheological and Structural Characteristics

Ferulated arabinoxylans were alkali-extracted from wheat bran at different incubation times (0.0, 0.5, 1.0, 1.5 and 2.0 h). Wheat bran ferulated arabinoxylans (WBAX) arabinose-to-xylose ratio, ferulic acid content, intrinsic viscosity and viscosimetric molecular weight values decreased as the incubation time of extraction increased. WBAX enzymatic cross-linking capability was affected by incubation time while an increase in WBAX concentration from 5 to 6% (w/v) favored gelation. The WBAX gels formed presented a macroporous structure with mesh size ranging from 40 to 119 nm and hardness values varying from 1.7 to 5 N

GenSeed-HMM: A tool for progressive assembly using profile HMMs as seeds and its application in Alpavirinae viral discovery from metagenomic data

This work reports the development of GenSeed-HMM, a program that implements seed-driven progressive assembly, an approach to reconstruct specific sequences from unassembled data, starting from short nucleotide or protein seed sequences or profile Hidden Markov Models (HMM). The program can use any one of a number of sequence assemblers. Assembly is performed in multiple steps and relatively few reads are used in each cycle, consequently the program demands low computational resources. As a proof-of-concept and to demonstrate the power of HMM-driven progressive assemblies, GenSeed-HMM was applied to metagenomic datasets in the search for diverse ssDNA bacteriophages from the recently described Alpavirinae subfamily. Profile HMMs were built using Alpavirinae-specific regions from multiple sequence alignments using either the viral protein 1 (VP1) (major capsid protein) or VP4 (genome replication initiation protein). These profile HMMs were used by GenSeed-HMM (running Newbler assembler) as seeds to reconstruct viral genomes from sequencing datasets of human fecal samples. All contigs obtained were annotated and taxonomically classified using similarity searches and phylogenetic analyses. The most specific profile HMM seed enabled the reconstruction of 45 partial or complete Alpavirinae genomic sequences. A comparison with conventional (global) assembly of the same original dataset, using Newbler in a standalone execution, revealed that GenSeed-HMM outperformed global genomic assembly in several metrics employed. This approach is capable of detecting organisms that have not been used in the construction of the profile HMM, which opens up the possibility of diagnosing novel viruses, without previous specific information, constituting a de novo diagnosis. Additional applications include, but are not limited to, the specific assembly of extrachromosomal elements such as plastid and mitochondrial genomes from metagenomic data. Profile HMM seeds can also be used to reconstruct specific protein coding genes for gene diversity studies, and to determine all possible gene variants present in a metagenomic sample. Such surveys could be useful to detect the emergence of drug-resistance variants in sensitive environments such as hospitals and animal production facilities, where antibiotics are regularly used. Finally, GenSeed-HMM can be used as an adjunct for gap closure on assembly finishing projects, by using multiple contig ends as anchored seeds

First Records for Spawning of Caribbean Acropora Species in Colombian MPAs

Estimates of Colombian Caribbean coral percent cover in the Southern Caribbean are consistent with those throughout the Caribbean Sea, which has declined to about 10% of historical levels in the last few decades. Human activities like destructive fishing techniques in the marine parks have degraded the reefs over the last few decades. Colombia’s Marine Protected Areas (MPAs) have thousands of square kilometers to map and patrol and few resources to devote to scientific and restoration efforts. Efforts to implement sexual reproduction techniques for restoration are starting to successfully propagate and settle corals on ceramic plates for reef deployment in the area but require more natural history information for large-scale implementation in restoration. Past observations of captive endangered coral Acropora cervicornis in the nursery of the Oceanario Islas del Rosario indicate spawning 6 days after the August full moon for the previous 3 years. Coral spawn collection from the wild reef was completed each night from 2 to 7 days after the full moon in August 2019, and resulted in the first observation of A. cervicornis spawning on natural reefs in Parque Nacional Natural Los Corales del Rosario y de San Bernardo, a 1,200 km2 underwater national park and MPA established in 1977. Additionally, coral spawn collection from the nursery reefs in August 2019 provide the first reported observations of spawning for endangered coral Acropora palmata in Colombia