Microfaunal soil food webs in Mediterranean semi-arid agroecosystems. Does organic management improve soil health?

Soil food webs, which are responsible for relevant ecological functions in agroecosystems such as nutrient cycling and pest and disease suppression, represent a crucial aspect of agricultural sustainability. We studied soil properties and microfaunal food web diversity and functioning in six paired organic and conventional fields located in Central Spain to assess the effects of organic farming on soil diversity and functioning in semi-arid conditions. We hypothesized that organic farming may enhance functioning of soil food webs. Our results showed larger differences between crop types, namely olive groves and vineyards, than between farming scheme, i.e. organic and conventional fields, and few benefits of organic farming in terms of soil fertility. Soil properties (total N, C, and P, available P and K, electrical conductivity, NH4+, NO3−, soil moisture, pH) tended to present higher values in vineyards than in olive groves and in conventional than in organic fields. Some plant-parasitic nematodes were associated to organic fields, especially in vineyards, and all soils fell within a degraded soil food web condition, with low Structure and Enrichment Index values. Nematode metabolic footprints showed relevant seasonal dynamics, with the more intensive herbivore activity in spring. We conclude that the lack of conventional pesticides and mineral fertilizers is probably not enough to improve soil conservation in semi-arid Mediterranean agroecosystems, and thus active soil conservation practices, as reduced tillage or cover cropping, are required to increase agroecosystem sustainability.Fundación Internacional para la Restauración de EcosistemasMinisterio de Economía y Competitivida

Type 2 diabetes mellitus and COVID-19: a narrative review

Type-2 diabetes mellitus (T2DM) is a chronic metabolic disorder. The incidence and prevalence of patients with T2DM are increasing worldwide, even reaching epidemic values in most high- and middle-income countries. T2DM could be a risk factor of developing complications in other diseases. Indeed, some studies suggest a bidirectional interaction between T2DM and COVID-19. A growing body of evidence shows that COVID-19 prognosis in individuals with T2DM is worse compared with those without. Moreover, various studies have reported the emergence of newly diagnosed patients with T2DM after SARS-CoV-2 infection. The most common treatments for T2DM may influence SARS-CoV-2 and their implication in infection is briefly discussed in this review. A better understanding of the link between TD2M and COVID-19 could proactively identify risk factors and, as a result, develop strategies to improve the prognosis for these patients

Protein degradation by E3 ubiquitin ligases in cancer stem cells

Review[Abstract] Cancer stem cells are a small subpopulation within the tumor with high capacity for self-renewal, differentiation and reconstitution of tumor heterogeneity. Cancer stem cells are major contributors of tumor initiation, metastasis and therapy resistance in cancer. Emerging evidence indicates that ubiquitination-mediated post-translational modification plays a fundamental role in the maintenance of cancer stem cell characteristics. In this review, we will discuss how protein degradation controlled by the E3 ubiquitin ligases plays a fundamental role in the self-renewal, maintenance and differentiation of cancer stem cells, highlighting the possibility to develop novel therapeutic strategies against E3 ubiquitin ligases targeting CSCs to fight cancer.Instituto de Salud Carlos III; PI18/00121Instituto de Salud Carlos III; PI21/00238Xunta de Galicia; IN607B2020/1

Fundamental Properties of O-Type Stars

We present a comprehensive analysis of high-resolution, far-UV HST/STIS, FUSE, and optical spectra of 17 O stars in the SMC. Our analysis is based on NLTE metal line-blanketed model atmospheres calculated with our NLTE code TLUSTY. We systematically explore the sensitivity of various UV and optical lines to different stellar parameters. We have obtained consistent fits of the UV and the optical spectrum to derive the effective temperature, surface gravity, surface composition, and microturbulent velocity of each star. Stellar radii, masses, luminosities and ages then follow. Similarly to more limited recent studies, we derive cooler temperatures than the standard Teff calibration of O stars. We propose a new calibration between the spectral type and effective temperature based on our results from UV metal lines as well as optical hydrogen and helium lines. For stars of the same spectral subtype, we find a general good agreement between Teff determinations obtained with TLUSTY, CMFGEN, and FASTWIND models. We derive ionizing luminosities that are smaller by a factor of 3 compared to luminosities inferred from previous standard calibrations. The chemical composition analysis reveals that the surface of about 3/4 of the program stars is moderately to strongly enriched in nitrogen, while showing the original helium, carbon, and oxygen abundances. Our results support the new stellar evolution models that predict that the surface of fast rotating stars becomes N-rich during the main sequence phase because of rotationally-induced mixing. Most stars exhibit the ``mass discrepancy'' problem. This discrepancy too is a result of fast rotation which lowers the measured effective gravity. Our study thus emphasizes the importance of rotation in our understanding of the properties of massive stars. (abridged)Comment: Submitted to Astrophysical Journal; 69 page

CLIMB-COVID: continuous integration supporting decentralised sequencing for SARS-CoV-2 genomic surveillance.

Funder: Wellcome TrustIn response to the ongoing SARS-CoV-2 pandemic in the UK, the COVID-19 Genomics UK (COG-UK) consortium was formed to rapidly sequence SARS-CoV-2 genomes as part of a national-scale genomic surveillance strategy. The network consists of universities, academic institutes, regional sequencing centres and the four UK Public Health Agencies. We describe the development and deployment of CLIMB-COVID, an encompassing digital infrastructure to address the challenge of collecting and integrating both genomic sequencing data and sample-associated metadata produced across the COG-UK network

Evaluating the effects of SARS-CoV-2 Spike mutation D614G on transmissibility and pathogenicity

SummaryGlobal dispersal and increasing frequency of the SARS-CoV-2 Spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of Spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large data set, well represented by both Spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the Spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant.</jats:p

Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large dataset, well represented by both spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant

Chemical characterization of some substituted hydroxyapatites

Synthetic multi-substituted hydroxyapatite nano powders containing silicon and or carbonate prepared by a wet chemical method. The process parameters are set up to allow the simultaneous substitution of carbonate and silicon ions in the place of phosphorus. The chemical and structural characterizations of the prepared powders are determined with the aid of; XRF, ICP, XRD and FTIR. The results show that, the ion substitution in the crystal lattice of HA caused a change in the unit cell dimensions and affected the degree of crystallization of the produced powders. The apatite formation abilityy of the prepared discs from the synthesized powders is determined by immersing in SBF solution for different periods. The degree of ion release was determined in the obtained solutions. The examined surface of the immersed discs under SEM and analyzed by CDS showed a more dense HA layer than those of un-substituted ones. The HA with the substituted silicon and carbonate ions, showed the highest solubility with greater rate of ion release, compared with carbonate-free powder. All prepared powders took sodium ion from the SBF solution during immersion, which was not recorded before

Epidemiological analysis of the first 1000 cases of SARS-CoV-2 lineage BA.1 (B.1.1.529, Omicron) compared with co-circulating Delta in Wales, UK

Background The Omicron (lineage B.1.1.529) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first reported in Wales, UK, on 3 December 2021. The aim of the study was to describe the first 1000 cases of the Omicron variant by demographic, vaccination status, travel and severe outcome status and compare this to contemporaneous cases of the Delta variant. Methods Testing, typing and contact tracing data were collected by Public Health Wales and analysis undertaken by the Communicable Disease Surveillance Centre (CDSC). Risk ratios for demographic factors and symptoms were calculated comparing Omicron cases to Delta cases identified over the same time period. Results By 14 December 2021, 1000 cases of the Omicron variant had been identified in Wales. Of the first 1000, just 3% of cases had a prior history of travel revealing rapid community transmission. A higher proportion of Omicron cases were identified in individuals aged 20–39, and most cases were double vaccinated (65.9%) or boosted (15.7%). Age-adjusted analysis also revealed that Omicron cases were less likely to be hospitalised (0.4%) or report symptoms (60.8%). Specifically a significant reduction was observed in the proportion of Omicron cases reporting anosmia (8.9%). Conclusion Key findings include a lower risk of anosmia and a reduced risk of hospitalisation in the first 1000 Omicron cases compared with co-circulating Delta cases. We also identify that existing measures for travel restrictions to control importations of new variants identified outside the United Kingdom did not prevent the rapid ingress of Omicron within Wales